UniProtKB - P23906 (IRF2_MOUSE)
Protein
Interferon regulatory factor 2
Gene
Irf2
Organism
Mus musculus (Mouse)
Status
Functioni
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 5 – 113 | IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST | 109 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cell population proliferation Source: InterPro
- defense response to virus Source: MGI
- immune system process Source: GO_Central
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Interferon regulatory factor 2Short name: IRF-2 |
Gene namesi | Name:Irf2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96591, Irf2 |
Subcellular locationi
Nucleus
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- focal adhesion Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154550 | 1 – 349 | Interferon regulatory factor 2Add BLAST | 349 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 75 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 78 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 137 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 137 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 166 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 225 | PhosphoserineBy similarity | 1 | |
Cross-linki | 260 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 293 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity |
Post-translational modificationi
Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity (By similarity).By similarity
The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter (By similarity).By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P23906 |
PaxDbi | P23906 |
PeptideAtlasi | P23906 |
PRIDEi | P23906 |
ProteomicsDBi | 267006 |
PTM databases
iPTMneti | P23906 |
PhosphoSitePlusi | P23906 |
Expressioni
Inductioni
By viruses and IFN.
Gene expression databases
Bgeei | ENSMUSG00000031627, Expressed in blood and 243 other tissues |
ExpressionAtlasi | P23906, baseline and differential |
Genevisiblei | P23906, MM |
Interactioni
Subunit structurei
Interacts with BRD7, IRF2BP1 and IRF2BP2.
Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 200785, 4 interactors |
IntActi | P23906, 1 interactor |
MINTi | P23906 |
STRINGi | 10090.ENSMUSP00000034041 |
Miscellaneous databases
RNActi | P23906, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P23906 |
SMRi | P23906 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23906 |
Family & Domainsi
Sequence similaritiesi
Belongs to the IRF family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | ENOG502QW7C, Eukaryota |
GeneTreei | ENSGT00940000159063 |
HOGENOMi | CLU_056386_0_0_1 |
InParanoidi | P23906 |
OMAi | SNLGMRN |
OrthoDBi | 734108at2759 |
PhylomeDBi | P23906 |
TreeFami | TF328512 |
Family and domain databases
CDDi | cd00103, IRF, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR019817, Interferon_reg_fac_CS IPR001346, Interferon_reg_fact_DNA-bd_dom IPR017431, IRF1/IRF2 IPR031218, IRF2 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11949, PTHR11949, 1 hit PTHR11949:SF22, PTHR11949:SF22, 1 hit |
Pfami | View protein in Pfam PF00605, IRF, 1 hit |
PIRSFi | PIRSF038196, IFN_RF1/2, 1 hit |
PRINTSi | PR00267, INTFRNREGFCT |
SMARTi | View protein in SMART SM00348, IRF, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS00601, IRF_1, 1 hit PS51507, IRF_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
P23906-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPVERMRMRP WLEEQINSNT IPGLKWLNKE KKIFQIPWMH AARHGWDVEK
60 70 80 90 100
DAPLFRNWAI HTGKHQPGID KPDPKTWKAN FRCAMNSLPD IEEVKDRSIK
110 120 130 140 150
KGNNAFRVYR MLPLSERPSK KGKKPKTEKE ERVKHIKQEP VESSLGLSNG
160 170 180 190 200
VSGFSPEYAV LTSAIKNEVD STVNIIVVGQ SHLDSNIEDQ EIVTNPPDIC
210 220 230 240 250
QVVEVTTESD DQPVSMSELY PLQISPVSSY AESETTDSVA SDEENAEGRP
260 270 280 290 300
HWRKRSIEGK QYLSNMGTRN TYLLPSMATF VTSNKPDLQV TIKEDSCPMP
310 320 330 340
YNSSWPPFTD LPLPAPVTPT PSSSRPDRET RASVIKKTSD ITQARVKSC
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GQW5 | A0A1B0GQW5_MOUSE | Interferon regulatory factor 2 | Irf2 | 138 | Annotation score: | ||
A0A140LHP8 | A0A140LHP8_MOUSE | Interferon regulatory factor 2 | Irf2 | 88 | Annotation score: | ||
A0A140LIX2 | A0A140LIX2_MOUSE | Interferon regulatory factor 2 | Irf2 | 118 | Annotation score: | ||
A0A140LJA4 | A0A140LJA4_MOUSE | Interferon regulatory factor 2 | Irf2 | 113 | Annotation score: | ||
A0A140LI82 | A0A140LI82_MOUSE | Interferon regulatory factor 2 | Irf2 | 180 | Annotation score: | ||
A0A1B0GQZ7 | A0A1B0GQZ7_MOUSE | Interferon regulatory factor 2 | Irf2 | 41 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03168 mRNA Translation: AAA39333.1 |
CCDSi | CCDS22295.1 |
RefSeqi | NP_032417.3, NM_008391.4 XP_006509351.1, XM_006509288.3 XP_006509352.1, XM_006509289.3 |
Genome annotation databases
Ensembli | ENSMUST00000034041; ENSMUSP00000034041; ENSMUSG00000031627 |
GeneIDi | 16363 |
KEGGi | mmu:16363 |
UCSCi | uc009lqo.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03168 mRNA Translation: AAA39333.1 |
CCDSi | CCDS22295.1 |
RefSeqi | NP_032417.3, NM_008391.4 XP_006509351.1, XM_006509288.3 XP_006509352.1, XM_006509289.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IRF | NMR | - | A | 2-113 | [»] | |
1IRG | NMR | - | A | 2-113 | [»] | |
2IRF | X-ray | 2.20 | G/H/I/J/K/L | 1-113 | [»] | |
BMRBi | P23906 | |||||
SMRi | P23906 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200785, 4 interactors |
IntActi | P23906, 1 interactor |
MINTi | P23906 |
STRINGi | 10090.ENSMUSP00000034041 |
PTM databases
iPTMneti | P23906 |
PhosphoSitePlusi | P23906 |
Proteomic databases
jPOSTi | P23906 |
PaxDbi | P23906 |
PeptideAtlasi | P23906 |
PRIDEi | P23906 |
ProteomicsDBi | 267006 |
Protocols and materials databases
Antibodypediai | 17374, 514 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034041; ENSMUSP00000034041; ENSMUSG00000031627 |
GeneIDi | 16363 |
KEGGi | mmu:16363 |
UCSCi | uc009lqo.2, mouse |
Organism-specific databases
CTDi | 3660 |
MGIi | MGI:96591, Irf2 |
Phylogenomic databases
eggNOGi | ENOG502QW7C, Eukaryota |
GeneTreei | ENSGT00940000159063 |
HOGENOMi | CLU_056386_0_0_1 |
InParanoidi | P23906 |
OMAi | SNLGMRN |
OrthoDBi | 734108at2759 |
PhylomeDBi | P23906 |
TreeFami | TF328512 |
Miscellaneous databases
BioGRID-ORCSi | 16363, 9 hits in 53 CRISPR screens |
ChiTaRSi | Irf2, mouse |
EvolutionaryTracei | P23906 |
PROi | PR:P23906 |
RNActi | P23906, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031627, Expressed in blood and 243 other tissues |
ExpressionAtlasi | P23906, baseline and differential |
Genevisiblei | P23906, MM |
Family and domain databases
CDDi | cd00103, IRF, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR019817, Interferon_reg_fac_CS IPR001346, Interferon_reg_fact_DNA-bd_dom IPR017431, IRF1/IRF2 IPR031218, IRF2 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11949, PTHR11949, 1 hit PTHR11949:SF22, PTHR11949:SF22, 1 hit |
Pfami | View protein in Pfam PF00605, IRF, 1 hit |
PIRSFi | PIRSF038196, IFN_RF1/2, 1 hit |
PRINTSi | PR00267, INTFRNREGFCT |
SMARTi | View protein in SMART SM00348, IRF, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS00601, IRF_1, 1 hit PS51507, IRF_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IRF2_MOUSE | |
Accessioni | P23906Primary (citable) accession number: P23906 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | April 7, 2021 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families