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UniProtKB - P23893 (GSA_ECOLI)
Protein
Glutamate-1-semialdehyde 2,1-aminomutase
Gene
hemL
Organism
Escherichia coli (strain K12)
Status
Functioni
Aminomutase that catalyzes the transfer of the amine group on carbon 2 of glutamate 1-semialdehyde to the adjacent carbon 1 to form 5-aminolevulinate.
2 PublicationsCatalytic activityi
Cofactori
pyridoxal 5'-phosphate2 Publications
: protoporphyrin-IX biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu). This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.
GO - Molecular functioni
- glutamate-1-semialdehyde 2,1-aminomutase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
- pyridoxal phosphate binding Source: InterPro
- transaminase activity Source: InterPro
GO - Biological processi
- porphyrin-containing compound biosynthetic process Source: GO_Central
- protoporphyrinogen IX biosynthetic process Source: EcoCyc
- tetrapyrrole biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Biological process | Porphyrin biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:GSAAMINOTRANS-MONOMER |
UniPathwayi | UPA00251;UER00317 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate-1-semialdehyde 2,1-aminomutase (EC:5.4.3.82 Publications)Short name: GSA Alternative name(s): Glutamate-1-semialdehyde aminotransferase Short name: GSA-AT |
Gene namesi | Name:hemL Synonyms:gsa, popC Ordered Locus Names:b0154, JW0150 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Cytosol
- cytosol Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 265 | K → R: 2% of wild-type activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120408 | 1 – 426 | Glutamate-1-semialdehyde 2,1-aminomutaseAdd BLAST | 426 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 265 | N6-(pyridoxal phosphate)lysineCurated | 1 |
Proteomic databases
jPOSTi | P23893 |
PaxDbi | P23893 |
PRIDEi | P23893 |
Expressioni
Inductioni
Induced by low extracellular levels of magnesium via the PhoQ/PhoP two-component regulatory system.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
P23893
With | #Exp. | IntAct |
---|---|---|
glcC [P0ACL5] | 4 | EBI-909193,EBI-1115389 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260833, 31 interactors 851232, 2 interactors |
DIPi | DIP-9886N |
IntActi | P23893, 2 interactors |
STRINGi | 511145.b0154 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.Curated
Phylogenomic databases
eggNOGi | COG0001, Bacteria |
HOGENOMi | CLU_016922_1_5_6 |
InParanoidi | P23893 |
PhylomeDBi | P23893 |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00375, HemL_aminotrans_3, 1 hit |
InterProi | View protein in InterPro IPR004639, 4pyrrol_synth_GluAld_NH2Trfase IPR005814, Aminotrans_3 IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00713, hemL, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P23893-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKSENLYSA ARELIPGGVN SPVRAFTGVG GTPLFIEKAD GAYLYDVDGK
60 70 80 90 100
AYIDYVGSWG PMVLGHNHPA IRNAVIEAAE RGLSFGAPTE MEVKMAQLVT
110 120 130 140 150
ELVPTMDMVR MVNSGTEATM SAIRLARGFT GRDKIIKFEG CYHGHADCLL
160 170 180 190 200
VKAGSGALTL GQPNSPGVPA DFAKYTLTCT YNDLASVRAA FEQYPQEIAC
210 220 230 240 250
IIVEPVAGNM NCVPPLPEFL PGLRALCDEF GALLIIDEVM TGFRVALAGA
260 270 280 290 300
QDYYGVVPDL TCLGKIIGGG MPVGAFGGRR DVMDALAPTG PVYQAGTLSG
310 320 330 340 350
NPIAMAAGFA CLNEVAQPGV HETLDELTTR LAEGLLEAAE EAGIPLVVNH
360 370 380 390 400
VGGMFGIFFT DAESVTCYQD VMACDVERFK RFFHMMLDEG VYLAPSAFEA
410 420
GFMSVAHSME DINNTIDAAR RVFAKL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | S → R (PubMed:1900346).Curated | 1 | |
Sequence conflicti | 2 | S → R (PubMed:8202364).Curated | 1 | |
Sequence conflicti | 9 | S → Q (PubMed:1900346).Curated | 1 | |
Sequence conflicti | 9 | S → Q (PubMed:8202364).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53696 Genomic DNA Translation: CAA37734.1 U00096 Genomic DNA Translation: AAC73265.1 AP009048 Genomic DNA Translation: BAB96731.2 U70214 Genomic DNA Translation: AAB08584.1 |
PIRi | B64739 |
RefSeqi | NP_414696.1, NC_000913.3 WP_000045315.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73265; AAC73265; b0154 BAB96731; BAB96731; BAB96731 |
GeneIDi | 946892 |
KEGGi | ecj:JW0150 eco:b0154 |
PATRICi | fig|1411691.4.peg.2126 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53696 Genomic DNA Translation: CAA37734.1 U00096 Genomic DNA Translation: AAC73265.1 AP009048 Genomic DNA Translation: BAB96731.2 U70214 Genomic DNA Translation: AAB08584.1 |
PIRi | B64739 |
RefSeqi | NP_414696.1, NC_000913.3 WP_000045315.1, NZ_LN832404.1 |
3D structure databases
SMRi | P23893 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260833, 31 interactors 851232, 2 interactors |
DIPi | DIP-9886N |
IntActi | P23893, 2 interactors |
STRINGi | 511145.b0154 |
Proteomic databases
jPOSTi | P23893 |
PaxDbi | P23893 |
PRIDEi | P23893 |
Genome annotation databases
EnsemblBacteriai | AAC73265; AAC73265; b0154 BAB96731; BAB96731; BAB96731 |
GeneIDi | 946892 |
KEGGi | ecj:JW0150 eco:b0154 |
PATRICi | fig|1411691.4.peg.2126 |
Organism-specific databases
EchoBASEi | EB0427 |
Phylogenomic databases
eggNOGi | COG0001, Bacteria |
HOGENOMi | CLU_016922_1_5_6 |
InParanoidi | P23893 |
PhylomeDBi | P23893 |
Enzyme and pathway databases
UniPathwayi | UPA00251;UER00317 |
BioCyci | EcoCyc:GSAAMINOTRANS-MONOMER |
Miscellaneous databases
PROi | PR:P23893 |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00375, HemL_aminotrans_3, 1 hit |
InterProi | View protein in InterPro IPR004639, 4pyrrol_synth_GluAld_NH2Trfase IPR005814, Aminotrans_3 IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00713, hemL, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GSA_ECOLI | |
Accessioni | P23893Primary (citable) accession number: P23893 Secondary accession number(s): P78277 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families