UniProtKB - P23890 (CADC_ECOLI)
Protein
Transcriptional activator CadC
Gene
cadC
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for Pcad induction, a promoter upstream of cadBA that is responsible for the pH-regulated expression of CadA and CadB. Probably acts as an activating transcription factor.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 3 – 102 | OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST | 100 |
GO - Molecular functioni
- DNA-binding transcription activator activity Source: GO_Central
- DNA-binding transcription factor activity Source: GO_Central
- phosphorelay response regulator activity Source: GO_Central
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- positive regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation, Two-component regulatory system |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00436 |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional activator CadC |
Gene namesi | Name:cadC Ordered Locus Names:b4133, JW4094 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell membrane Curated; Single-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 155 – 180 | HelicalSequence analysisAdd BLAST | 26 |
GO - Cellular componenti
- cytosol Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: EcoCyc
- protein-DNA complex Source: GO_Central
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Loss of Pcad activity.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 120 – 124 | PPPIP → AAAIS: Significantly reduced dependence on EF-P for translation, increased levels of protein in an efp-strain. 1 Publication | 5 | |
Mutagenesisi | 121 | P → A: Reduced dependence on EF-P for translation, increased levels of protein in an efp- strain. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081345 | 1 – 512 | Transcriptional activator CadCAdd BLAST | 512 |
Proteomic databases
PaxDbi | P23890 |
PRIDEi | P23890 |
Expressioni
Inductioni
Induction requires low pH plus exogenous lysine as sensed by LysP as well as EF-P for translation. This requirement is reduced as the number of consecutive Pro residues in the protein is decreased.1 Publication
Interactioni
Binary interactionsi
P23890
With | #Exp. | IntAct |
---|---|---|
lysP [P25737] | 2 | EBI-6401662,EBI-6401655 |
Protein-protein interaction databases
BioGRIDi | 4260781, 7 interactors 852945, 1 interactor |
DIPi | DIP-9239N |
IntActi | P23890, 1 interactor |
STRINGi | 511145.b4133 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23890 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23890 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 120 – 124 | Poly-Pro | 5 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3710, Bacteria |
HOGENOMi | CLU_040424_0_0_6 |
Family and domain databases
CDDi | cd00383, trans_reg_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR040970, CadC_C1 IPR001867, OmpR/PhoB-type_DNA-bd IPR016032, Sig_transdc_resp-reg_C-effctor IPR011990, TPR-like_helical_dom_sf IPR039420, WalR-like IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR26402, PTHR26402, 1 hit |
Pfami | View protein in Pfam PF18500, CadC_C1, 1 hit PF00486, Trans_reg_C, 1 hit |
SMARTi | View protein in SMART SM00862, Trans_reg_C, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit |
PROSITEi | View protein in PROSITE PS51755, OMPR_PHOB, 1 hit |
i Sequence
Sequence statusi: Complete.
P23890-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQQPVVRVGE WLVTPSINQI SRNGRQLTLE PRLIDLLVFF AQHSGEVLSR
60 70 80 90 100
DELIDNVWKR SIVTNHVVTQ SISELRKSLK DNDEDSPVYI ATVPKRGYKL
110 120 130 140 150
MVPVIWYSEE EGEEIMLSSP PPIPEAVPAT DSPSHSLNIQ NTATPPEQSP
160 170 180 190 200
VKSKRFTTFW VWFFFLLSLG ICVALVAFSS LDTRLPMSKS RILLNPRDID
210 220 230 240 250
INMVNKSCNS WSSPYQLSYA IGVGDLVATS LNTFSTFMVH DKINYNIDEP
260 270 280 290 300
SSSGKTLSIA FVNQRQYRAQ QCFMSIKLVD NADGSTMLDK RYVITNGNQL
310 320 330 340 350
AIQNDLLESL SKALNQPWPQ RMQETLQKIL PHRGALLTNF YQAHDYLLHG
360 370 380 390 400
DDKSLNRASE LLGEIVQSSP EFTYARAEKA LVDIVRHSQH PLDEKQLAAL
410 420 430 440 450
NTEIDNIVTL PELNNLSIIY QIKAVSALVK GKTDESYQAI NTGIDLEMSW
460 470 480 490 500
LNYVLLGKVY EMKGMNREAA DAYLTAFNLR PGANTLYWIE NGIFQTSVPY
510
VVPYLDKFLA SE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M67452 Genomic DNA Translation: AAA23531.1 U14003 Genomic DNA Translation: AAA97033.1 U00096 Genomic DNA Translation: AAC77094.1 AP009048 Genomic DNA Translation: BAE78136.1 |
PIRi | C41968 |
RefSeqi | NP_418557.1, NC_000913.3 WP_001187173.1, NZ_SSZK01000018.1 |
Genome annotation databases
EnsemblBacteriai | AAC77094; AAC77094; b4133 BAE78136; BAE78136; BAE78136 |
GeneIDi | 948653 |
KEGGi | ecj:JW4094 eco:b4133 |
PATRICi | fig|1411691.4.peg.2566 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M67452 Genomic DNA Translation: AAA23531.1 U14003 Genomic DNA Translation: AAA97033.1 U00096 Genomic DNA Translation: AAC77094.1 AP009048 Genomic DNA Translation: BAE78136.1 |
PIRi | C41968 |
RefSeqi | NP_418557.1, NC_000913.3 WP_001187173.1, NZ_SSZK01000018.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3LY7 | X-ray | 1.80 | A | 188-512 | [»] | |
3LY8 | X-ray | 1.90 | A | 188-512 | [»] | |
3LY9 | X-ray | 2.20 | A | 188-512 | [»] | |
3LYA | X-ray | 2.30 | A | 188-512 | [»] | |
5JU7 | X-ray | 2.05 | A | 2-107 | [»] | |
SMRi | P23890 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260781, 7 interactors 852945, 1 interactor |
DIPi | DIP-9239N |
IntActi | P23890, 1 interactor |
STRINGi | 511145.b4133 |
Proteomic databases
PaxDbi | P23890 |
PRIDEi | P23890 |
Protocols and materials databases
DNASUi | 948653 |
Genome annotation databases
EnsemblBacteriai | AAC77094; AAC77094; b4133 BAE78136; BAE78136; BAE78136 |
GeneIDi | 948653 |
KEGGi | ecj:JW4094 eco:b4133 |
PATRICi | fig|1411691.4.peg.2566 |
Organism-specific databases
EchoBASEi | EB0131 |
Phylogenomic databases
eggNOGi | COG3710, Bacteria |
HOGENOMi | CLU_040424_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00436 |
Miscellaneous databases
EvolutionaryTracei | P23890 |
PROi | PR:P23890 |
Family and domain databases
CDDi | cd00383, trans_reg_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR040970, CadC_C1 IPR001867, OmpR/PhoB-type_DNA-bd IPR016032, Sig_transdc_resp-reg_C-effctor IPR011990, TPR-like_helical_dom_sf IPR039420, WalR-like IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR26402, PTHR26402, 1 hit |
Pfami | View protein in Pfam PF18500, CadC_C1, 1 hit PF00486, Trans_reg_C, 1 hit |
SMARTi | View protein in SMART SM00862, Trans_reg_C, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit |
PROSITEi | View protein in PROSITE PS51755, OMPR_PHOB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CADC_ECOLI | |
Accessioni | P23890Primary (citable) accession number: P23890 Secondary accession number(s): Q2M6H0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references