UniProtKB - P23882 (FMT_ECOLI)
Protein
Methionyl-tRNA formyltransferase
Gene
fmt
Organism
Escherichia coli (strain K12)
Status
Functioni
Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.UniRule annotation3 Publications
Catalytic activityi
- (6S)-10-formyltetrahydrofolate + L-methionyl-tRNAfMet = (6S)-5,6,7,8-tetrahydrofolate + H+ + N-formyl-L-methionyl-tRNAfMetUniRule annotation2 PublicationsEC:2.1.2.9UniRule annotation2 Publications
Activity regulationi
Activity is optimum in the presence of Mg2+ and K+.1 Publication
Kineticsi
- KM=13.5 µM for 10-formyltetrahydrofolate1 Publication
- KM=0.35 µM for L-methionyl-tRNA(fMet)1 Publication
GO - Molecular functioni
- methionyl-tRNA formyltransferase activity Source: EcoCyc
GO - Biological processi
- charged-tRNA amino acid modification Source: EcoCyc
- conversion of methionyl-tRNA to N-formyl-methionyl-tRNA Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Protein biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11268-MONOMER MetaCyc:EG11268-MONOMER |
BRENDAi | 2.1.2.9, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Methionyl-tRNA formyltransferaseUniRule annotationCurated (EC:2.1.2.9UniRule annotation2 Publications)Alternative name(s): Met-tRNA(fMet) formyltransferase1 Publication |
Gene namesi | Name:fmt1 PublicationUniRule annotation Synonyms:yhdD Ordered Locus Names:b3288, JW3249 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000082959 | 2 – 315 | Methionyl-tRNA formyltransferaseAdd BLAST | 314 |
Proteomic databases
jPOSTi | P23882 |
PaxDbi | P23882 |
PRIDEi | P23882 |
2D gel databases
SWISS-2DPAGEi | P23882 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4263429, 45 interactors 852091, 1 interactor |
DIPi | DIP-9668N |
IntActi | P23882, 12 interactors |
STRINGi | 511145.b3288 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23882 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23882 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 189 | N-terminal domain2 PublicationsAdd BLAST | 188 | |
Regioni | 113 – 116 | Tetrahydrofolate (THF) bindingUniRule annotation | 4 | |
Regioni | 210 – 315 | C-terminal domain2 PublicationsAdd BLAST | 106 |
Domaini
Composed of an N- and a C-terminal domain. The N-terminal domain carries the tetrahydrofolate (THF)-binding site and the C-terminal domain is presumably involved in positioning the Met-tRNA substrate for the formylation reaction.2 Publications
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0223, Bacteria |
HOGENOMi | CLU_033347_1_2_6 |
InParanoidi | P23882 |
PhylomeDBi | P23882 |
Family and domain databases
CDDi | cd08646, FMT_core_Met-tRNA-FMT_N, 1 hit cd08704, Met_tRNA_FMT_C, 1 hit |
Gene3Di | 3.10.25.10, 1 hit |
HAMAPi | MF_00182, Formyl_trans, 1 hit |
InterProi | View protein in InterPro IPR005794, Fmt IPR005793, Formyl_trans_C IPR037022, Formyl_trans_C_sf IPR002376, Formyl_transf_N IPR036477, Formyl_transf_N_sf IPR011034, Formyl_transferase-like_C_sf IPR001555, GART_AS IPR044135, Met-tRNA-FMT_C IPR041711, Met-tRNA-FMT_N |
Pfami | View protein in Pfam PF02911, Formyl_trans_C, 1 hit PF00551, Formyl_trans_N, 1 hit |
SUPFAMi | SSF50486, SSF50486, 1 hit SSF53328, SSF53328, 1 hit |
TIGRFAMsi | TIGR00460, fmt, 1 hit |
PROSITEi | View protein in PROSITE PS00373, GART, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P23882-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSESLRIIFA GTPDFAARHL DALLSSGHNV VGVFTQPDRP AGRGKKLMPS
60 70 80 90 100
PVKVLAEEKG LPVFQPVSLR PQENQQLVAE LQADVMVVVA YGLILPKAVL
110 120 130 140 150
EMPRLGCINV HGSLLPRWRG AAPIQRSLWA GDAETGVTIM QMDVGLDTGD
160 170 180 190 200
MLYKLSCPIT AEDTSGTLYD KLAELGPQGL ITTLKQLADG TAKPEVQDET
210 220 230 240 250
LVTYAEKLSK EEARIDWSLS AAQLERCIRA FNPWPMSWLE IEGQPVKVWK
260 270 280 290 300
ASVIDTATNA APGTILEANK QGIQVATGDG ILNLLSLQPA GKKAMSAQDL
310
LNSRREWFVP GNRLV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X63666 Genomic DNA Translation: CAA45207.1 X77091 Genomic DNA Translation: CAA54368.1 U18997 Genomic DNA Translation: AAA58085.1 U00096 Genomic DNA Translation: AAC76313.1 AP009048 Genomic DNA Translation: BAE78004.1 X00767 Genomic DNA Translation: CAA25339.1 Sequence problems. Y10307 Genomic DNA Translation: CAA71358.1 |
PIRi | S23108 |
RefSeqi | NP_417746.1, NC_000913.3 WP_000004473.1, NZ_SSZK01000040.1 |
Genome annotation databases
EnsemblBacteriai | AAC76313; AAC76313; b3288 BAE78004; BAE78004; BAE78004 |
GeneIDi | 947779 |
KEGGi | ecj:JW3249 eco:b3288 |
PATRICi | fig|1411691.4.peg.3444 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X63666 Genomic DNA Translation: CAA45207.1 X77091 Genomic DNA Translation: CAA54368.1 U18997 Genomic DNA Translation: AAA58085.1 U00096 Genomic DNA Translation: AAC76313.1 AP009048 Genomic DNA Translation: BAE78004.1 X00767 Genomic DNA Translation: CAA25339.1 Sequence problems. Y10307 Genomic DNA Translation: CAA71358.1 |
PIRi | S23108 |
RefSeqi | NP_417746.1, NC_000913.3 WP_000004473.1, NZ_SSZK01000040.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FMT | X-ray | 2.00 | A/B | 2-315 | [»] | |
2FMT | X-ray | 2.80 | A/B | 2-315 | [»] | |
SMRi | P23882 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263429, 45 interactors 852091, 1 interactor |
DIPi | DIP-9668N |
IntActi | P23882, 12 interactors |
STRINGi | 511145.b3288 |
Chemistry databases
DrugBanki | DB04464, N-Formylmethionine |
2D gel databases
SWISS-2DPAGEi | P23882 |
Proteomic databases
jPOSTi | P23882 |
PaxDbi | P23882 |
PRIDEi | P23882 |
Genome annotation databases
EnsemblBacteriai | AAC76313; AAC76313; b3288 BAE78004; BAE78004; BAE78004 |
GeneIDi | 947779 |
KEGGi | ecj:JW3249 eco:b3288 |
PATRICi | fig|1411691.4.peg.3444 |
Organism-specific databases
EchoBASEi | EB1247 |
Phylogenomic databases
eggNOGi | COG0223, Bacteria |
HOGENOMi | CLU_033347_1_2_6 |
InParanoidi | P23882 |
PhylomeDBi | P23882 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11268-MONOMER MetaCyc:EG11268-MONOMER |
BRENDAi | 2.1.2.9, 2026 |
Miscellaneous databases
EvolutionaryTracei | P23882 |
PROi | PR:P23882 |
Family and domain databases
CDDi | cd08646, FMT_core_Met-tRNA-FMT_N, 1 hit cd08704, Met_tRNA_FMT_C, 1 hit |
Gene3Di | 3.10.25.10, 1 hit |
HAMAPi | MF_00182, Formyl_trans, 1 hit |
InterProi | View protein in InterPro IPR005794, Fmt IPR005793, Formyl_trans_C IPR037022, Formyl_trans_C_sf IPR002376, Formyl_transf_N IPR036477, Formyl_transf_N_sf IPR011034, Formyl_transferase-like_C_sf IPR001555, GART_AS IPR044135, Met-tRNA-FMT_C IPR041711, Met-tRNA-FMT_N |
Pfami | View protein in Pfam PF02911, Formyl_trans_C, 1 hit PF00551, Formyl_trans_N, 1 hit |
SUPFAMi | SSF50486, SSF50486, 1 hit SSF53328, SSF53328, 1 hit |
TIGRFAMsi | TIGR00460, fmt, 1 hit |
PROSITEi | View protein in PROSITE PS00373, GART, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FMT_ECOLI | |
Accessioni | P23882Primary (citable) accession number: P23882 Secondary accession number(s): P77040, Q2M6V2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 183 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families