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UniProtKB - P23871 (HEMH_ECOLI)
Protein
Ferrochelatase
Gene
hemH
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the ferrous insertion into protoporphyrin IX.
UniRule annotationCatalytic activityi
- EC:4.99.1.1UniRule annotation
: protoheme biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes protoheme from protoporphyrin-IX.UniRule annotation This subpathway is part of the pathway protoheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoheme from protoporphyrin-IX, the pathway protoheme biosynthesis and in Porphyrin-containing compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 194 | IronUniRule annotation | 1 | |
Metal bindingi | 275 | IronUniRule annotation | 1 |
GO - Molecular functioni
- ferrochelatase activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- heme biosynthetic process Source: EcoliWiki
- protoporphyrinogen IX metabolic process Source: EcoliWiki
- response to light stimulus Source: EcoliWiki
Keywordsi
Molecular function | Lyase |
Biological process | Heme biosynthesis, Porphyrin biosynthesis |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PROTOHEME-FERROCHELAT-MONOMER |
UniPathwayi | UPA00252;UER00325 |
Names & Taxonomyi
Protein namesi | Recommended name: FerrochelataseUniRule annotation (EC:4.99.1.1UniRule annotation)Alternative name(s): Heme synthaseUniRule annotation Protoheme ferro-lyaseUniRule annotation |
Gene namesi | Name:hemHUniRule annotation Synonyms:popA, visA Ordered Locus Names:b0475, JW0464 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells are sensitive to visible light (which is lethal).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000175138 | 1 – 320 | FerrochelataseAdd BLAST | 320 |
Proteomic databases
jPOSTi | P23871 |
PaxDbi | P23871 |
PRIDEi | P23871 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 4259849, 7 interactors 851849, 1 interactor |
IntActi | P23871, 9 interactors |
STRINGi | 511145.b0475 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0276, Bacteria |
HOGENOMi | CLU_018884_0_0_6 |
InParanoidi | P23871 |
PhylomeDBi | P23871 |
Family and domain databases
CDDi | cd00419, Ferrochelatase_C, 1 hit cd03411, Ferrochelatase_N, 1 hit |
HAMAPi | MF_00323, Ferrochelatase, 1 hit |
InterProi | View protein in InterPro IPR001015, Ferrochelatase IPR019772, Ferrochelatase_AS IPR033644, Ferrochelatase_C IPR033659, Ferrochelatase_N |
PANTHERi | PTHR11108, PTHR11108, 1 hit |
Pfami | View protein in Pfam PF00762, Ferrochelatase, 1 hit |
TIGRFAMsi | TIGR00109, hemH, 1 hit |
PROSITEi | View protein in PROSITE PS00534, FERROCHELATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P23871-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRQTKTGILL ANLGTPDAPT PEAVKRYLKQ FLSDRRVVDT SRLLWWPLLR
60 70 80 90 100
GVILPLRSPR VAKLYASVWM EGGSPLMVYS RQQQQALAQR LPEMPVALGM
110 120 130 140 150
SYGSPSLESA VDELLAEHVD HIVVLPLYPQ FSCSTVGAVW DELARILARK
160 170 180 190 200
RSIPGISFIR DYADNHDYIN ALANSVRASF AKHGEPDLLL LSYHGIPQRY
210 220 230 240 250
ADEGDDYPQR CRTTTRELAS ALGMAPEKVM MTFQSRFGRE PWLMPYTDET
260 270 280 290 300
LKMLGEKGVG HIQVMCPGFA ADCLETLEEI AEQNREVFLG AGGKKYEYIP
310 320
ALNATPEHIE MMANLVAAYR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 54 | L → F in BAA14304 (PubMed:2051480).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D90259 Genomic DNA Translation: BAA14304.1 U82664 Genomic DNA Translation: AAB40229.1 U00096 Genomic DNA Translation: AAC73577.1 AP009048 Genomic DNA Translation: BAE76254.1 |
PIRi | B64778 |
RefSeqi | NP_415008.1, NC_000913.3 WP_001250103.1, NZ_SSZK01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC73577; AAC73577; b0475 BAE76254; BAE76254; BAE76254 |
GeneIDi | 947532 |
KEGGi | ecj:JW0464 eco:b0475 |
PATRICi | fig|1411691.4.peg.1801 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D90259 Genomic DNA Translation: BAA14304.1 U82664 Genomic DNA Translation: AAB40229.1 U00096 Genomic DNA Translation: AAC73577.1 AP009048 Genomic DNA Translation: BAE76254.1 |
PIRi | B64778 |
RefSeqi | NP_415008.1, NC_000913.3 WP_001250103.1, NZ_SSZK01000009.1 |
3D structure databases
SMRi | P23871 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259849, 7 interactors 851849, 1 interactor |
IntActi | P23871, 9 interactors |
STRINGi | 511145.b0475 |
Proteomic databases
jPOSTi | P23871 |
PaxDbi | P23871 |
PRIDEi | P23871 |
Genome annotation databases
EnsemblBacteriai | AAC73577; AAC73577; b0475 BAE76254; BAE76254; BAE76254 |
GeneIDi | 947532 |
KEGGi | ecj:JW0464 eco:b0475 |
PATRICi | fig|1411691.4.peg.1801 |
Organism-specific databases
EchoBASEi | EB0426 |
Phylogenomic databases
eggNOGi | COG0276, Bacteria |
HOGENOMi | CLU_018884_0_0_6 |
InParanoidi | P23871 |
PhylomeDBi | P23871 |
Enzyme and pathway databases
UniPathwayi | UPA00252;UER00325 |
BioCyci | EcoCyc:PROTOHEME-FERROCHELAT-MONOMER |
Miscellaneous databases
PROi | PR:P23871 |
Family and domain databases
CDDi | cd00419, Ferrochelatase_C, 1 hit cd03411, Ferrochelatase_N, 1 hit |
HAMAPi | MF_00323, Ferrochelatase, 1 hit |
InterProi | View protein in InterPro IPR001015, Ferrochelatase IPR019772, Ferrochelatase_AS IPR033644, Ferrochelatase_C IPR033659, Ferrochelatase_N |
PANTHERi | PTHR11108, PTHR11108, 1 hit |
Pfami | View protein in Pfam PF00762, Ferrochelatase, 1 hit |
TIGRFAMsi | TIGR00109, hemH, 1 hit |
PROSITEi | View protein in PROSITE PS00534, FERROCHELATASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HEMH_ECOLI | |
Accessioni | P23871Primary (citable) accession number: P23871 Secondary accession number(s): P78232, Q2MBV2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 158 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families