UniProtKB - P23869 (PPIB_ECOLI)
Protein
Peptidyl-prolyl cis-trans isomerase B
Gene
ppiB
Organism
Escherichia coli (strain K12)
Status
Functioni
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Catalytic activityi
- EC:5.2.1.8
Activity regulationi
Inhibition by cyclosporin A with a Ki of 25 to 50 mu-mol, a concentration 100-fold higher than that required for eukaryotic PPIases.
GO - Molecular functioni
- peptidyl-prolyl cis-trans isomerase activity Source: EcoCyc
GO - Biological processi
- protein folding Source: InterPro
Keywordsi
Molecular function | Isomerase, Rotamase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10758-MONOMER MetaCyc:EG10758-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Peptidyl-prolyl cis-trans isomerase B (EC:5.2.1.8)Short name: PPIase B Alternative name(s): Rotamase B |
Gene namesi | Name:ppiB Ordered Locus Names:b0525, JW0514 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064202 | 1 – 164 | Peptidyl-prolyl cis-trans isomerase BAdd BLAST | 164 |
Proteomic databases
jPOSTi | P23869 |
PaxDbi | P23869 |
PRIDEi | P23869 |
2D gel databases
SWISS-2DPAGEi | P23869 |
Interactioni
Binary interactionsi
P23869
With | #Exp. | IntAct |
---|---|---|
rhsC [P16918] | 6 | EBI-555935,EBI-546818 |
Protein-protein interaction databases
BioGRIDi | 4262014, 40 interactors 853282, 1 interactor |
DIPi | DIP-10546N |
IntActi | P23869, 23 interactors |
STRINGi | 511145.b0525 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P23869 |
SMRi | P23869 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23869 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 162 | PPIase cyclophilin-typePROSITE-ProRule annotationAdd BLAST | 162 |
Sequence similaritiesi
Belongs to the cyclophilin-type PPIase family.Curated
Phylogenomic databases
eggNOGi | COG0652, Bacteria |
HOGENOMi | CLU_012062_16_9_6 |
InParanoidi | P23869 |
PhylomeDBi | P23869 |
Family and domain databases
Gene3Di | 2.40.100.10, 1 hit |
InterProi | View protein in InterPro IPR029000, Cyclophilin-like_dom_sf IPR024936, Cyclophilin-type_PPIase IPR020892, Cyclophilin-type_PPIase_CS IPR002130, Cyclophilin-type_PPIase_dom |
Pfami | View protein in Pfam PF00160, Pro_isomerase, 1 hit |
PIRSFi | PIRSF001467, Peptidylpro_ismrse, 1 hit |
PRINTSi | PR00153, CSAPPISMRASE |
SUPFAMi | SSF50891, SSF50891, 1 hit |
PROSITEi | View protein in PROSITE PS00170, CSA_PPIASE_1, 1 hit PS50072, CSA_PPIASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P23869-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVTFHTNHGD IVIKTFDDKA PETVKNFLDY CREGFYNNTI FHRVINGFMI
60 70 80 90 100
QGGGFEPGMK QKATKEPIKN EANNGLKNTR GTLAMARTQA PHSATAQFFI
110 120 130 140 150
NVVDNDFLNF SGESLQGWGY CVFAEVVDGM DVVDKIKGVA TGRSGMHQDV
160
PKEDVIIESV TVSE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 132 | V → E in AAA23453 (PubMed:2007139).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55430 Genomic DNA Translation: AAA23453.1 U00096 Genomic DNA Translation: AAC73627.1 AP009048 Genomic DNA Translation: BAE76302.1 X59293 Genomic DNA Translation: CAA41982.1 |
PIRi | D64784, CSECB |
RefSeqi | NP_415058.1, NC_000913.3 WP_000255997.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73627; AAC73627; b0525 BAE76302; BAE76302; BAE76302 |
GeneIDi | 57731374 949038 |
KEGGi | ecj:JW0514 eco:b0525 |
PATRICi | fig|1411691.4.peg.1753 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55430 Genomic DNA Translation: AAA23453.1 U00096 Genomic DNA Translation: AAC73627.1 AP009048 Genomic DNA Translation: BAE76302.1 X59293 Genomic DNA Translation: CAA41982.1 |
PIRi | D64784, CSECB |
RefSeqi | NP_415058.1, NC_000913.3 WP_000255997.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1LOP | X-ray | 1.80 | A | 1-164 | [»] | |
2NUL | X-ray | 2.10 | A | 1-164 | [»] | |
2RS4 | NMR | - | A | 1-164 | [»] | |
BMRBi | P23869 | |||||
SMRi | P23869 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262014, 40 interactors 853282, 1 interactor |
DIPi | DIP-10546N |
IntActi | P23869, 23 interactors |
STRINGi | 511145.b0525 |
2D gel databases
SWISS-2DPAGEi | P23869 |
Proteomic databases
jPOSTi | P23869 |
PaxDbi | P23869 |
PRIDEi | P23869 |
Genome annotation databases
EnsemblBacteriai | AAC73627; AAC73627; b0525 BAE76302; BAE76302; BAE76302 |
GeneIDi | 57731374 949038 |
KEGGi | ecj:JW0514 eco:b0525 |
PATRICi | fig|1411691.4.peg.1753 |
Organism-specific databases
EchoBASEi | EB0751 |
Phylogenomic databases
eggNOGi | COG0652, Bacteria |
HOGENOMi | CLU_012062_16_9_6 |
InParanoidi | P23869 |
PhylomeDBi | P23869 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10758-MONOMER MetaCyc:EG10758-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P23869 |
PROi | PR:P23869 |
Family and domain databases
Gene3Di | 2.40.100.10, 1 hit |
InterProi | View protein in InterPro IPR029000, Cyclophilin-like_dom_sf IPR024936, Cyclophilin-type_PPIase IPR020892, Cyclophilin-type_PPIase_CS IPR002130, Cyclophilin-type_PPIase_dom |
Pfami | View protein in Pfam PF00160, Pro_isomerase, 1 hit |
PIRSFi | PIRSF001467, Peptidylpro_ismrse, 1 hit |
PRINTSi | PR00153, CSAPPISMRASE |
SUPFAMi | SSF50891, SSF50891, 1 hit |
PROSITEi | View protein in PROSITE PS00170, CSA_PPIASE_1, 1 hit PS50072, CSA_PPIASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPIB_ECOLI | |
Accessioni | P23869Primary (citable) accession number: P23869 Secondary accession number(s): P78052, Q2MBQ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families