Tail-specific protease

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityⁱ

• The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala. EC:3.4.21.102

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• endopeptidase activity Source: EcoliWiki <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "C-terminal specific protein degradation: activity and substrate specificity of the Tsp protease."
    Keiler K.C., Silber K.R., Downard K.M., Papayannopoulos I.A., Biemann K., Sauer R.T.
    Protein Sci. 4:1507-1515(1995) [PubMed] [Europe PMC] [Abstract]
• serine-type peptidase activity Source: UniProtKB-KW

GO - Biological processⁱ

• protein catabolic process Source: EcoliWikiInferred from direct assayⁱ
  • "C-terminal specific protein degradation: activity and substrate specificity of the Tsp protease."
    Keiler K.C., Silber K.R., Downard K.M., Papayannopoulos I.A., Biemann K., Sauer R.T.
    Protein Sci. 4:1507-1515(1995) [PubMed] [Europe PMC] [Abstract]
• proteolysis Source: EcoliWikiInferred from direct assayⁱ
  • "C-terminal specific protein degradation: activity and substrate specificity of the Tsp protease."
    Keiler K.C., Silber K.R., Downard K.M., Papayannopoulos I.A., Biemann K., Sauer R.T.
    Protein Sci. 4:1507-1515(1995) [PubMed] [Europe PMC] [Abstract]
• response to antibiotic Source: EcoCyc <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • Ref.6

    "Multiple antibiotic susceptibility associated with inactivation of the prc gene."
    Seoane A., Sabbaj A., McMurry L.M., Levy S.B.
    J. Bacteriol. 174:7844-7847(1992) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 330-403.
• signal transduction Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief. Bioinformatics 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

GO - Cellular componentⁱ

• outer membrane-bounded periplasmic space Source: EcoCycInferred from direct assayⁱ
  • Ref.1

    "Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3."
    Hara H., Yamamoto Y., Higashitani A., Suzuki H., Nishimura Y.
    J. Bacteriol. 173:4799-4813(1991) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
• plasma membrane Source: UniProtKB-SubCell

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingⁱ: The displayed sequence is further processed into a mature form.

        10         20         30         40         50
MNMFFRLTAL AGLLAIAGQT FAVEDITRAD QIPVLKEETQ HATVSERVTS 
        60         70         80         90        100
RFTRSHYRQF DLDQAFSAKI FDRYLNLLDY SHNVLLASDV EQFAKKKTEL 
       110        120        130        140        150
GDELRSGKLD VFYDLYNLAQ KRRFERYQYA LSVLEKPMDF TGNDTYNLDR 
       160        170        180        190        200
SKAPWPKNEA ELNALWDSKV KFDELSLKLT GKTDKEIRET LTRRYKFAIR 
       210        220        230        240        250
RLAQTNSEDV FSLAMTAFAR EIDPHTNYLS PRNTEQFNTE MSLSLEGIGA 
       260        270        280        290        300
VLQMDDDYTV INSMVAGGPA AKSKAISVGD KIVGVGQTGK PMVDVIGWRL 
       310        320        330        340        350
DDVVALIKGP KGSKVRLEIL PAGKGTKTRT VTLTRERIRL EDRAVKMSVK 
       360        370        380        390        400
TVGKEKVGVL DIPGFYVGLT DDVKVQLQKL EKQNVSSVII DLRSNGGGAL 
       410        420        430        440        450
TEAVSLSGLF IPAGPIVQVR DNNGKVREDS DTDGQVFYKG PLVVLVDRFS 
       460        470        480        490        500
ASASEIFAAA MQDYGRALVV GEPTFGKGTV QQYRSLNRIY DQMLRPEWPA 
       510        520        530        540        550
LGSVQYTIQK FYRVNGGSTQ RKGVTPDIIM PTGNEETETG EKFEDNALPW 
       560        570        580        590        600
DSIDAATYVK SGDLTAFEPE LLKEHNARIA KDPEFQNIMK DIARFNAMKD 
       610        620        630        640        650
KRNIVSLNYA VREKENNEDD ATRLARLNER FKREGKPELK KLDDLPKDYQ 
       660        670        680 
EPDPYLDETV NIALDLAKLE KARPAEQPAP VK                    

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautionⁱ

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Peptidase families
   Classification of peptidase families and list of entries
2. Escherichia coli
   Escherichia coli (strain K12): entries and cross-references to EcoGene
3. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
4. SIMILARITY comments
   Index of protein domains and families