UniProtKB - P23865 (PRC_ECOLI)
Protein
Tail-specific protease
Gene
prc
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the cleavage of a C-terminal peptide of 11 residues from the precursor form of penicillin-binding protein 3 (PBP3). May be involved in protection of the bacterium from thermal and osmotic stresses.
Catalytic activityi
- The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala. EC:3.4.21.102
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 452 | Charge relay system | 1 | |
Active sitei | 463 | Charge relay systemCurated | 1 | |
Active sitei | 477 | Charge relay system | 1 |
GO - Molecular functioni
- endopeptidase activity Source: EcoliWiki
- serine-type endopeptidase activity Source: UniProtKB-EC
GO - Biological processi
- protein catabolic process Source: EcoliWiki
- proteolysis Source: EcoliWiki
- response to antibiotic Source: EcoCyc
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10760-MONOMER MetaCyc:EG10760-MONOMER |
BRENDAi | 3.4.21.102, 2026 |
Protein family/group databases
MEROPSi | S41.001 |
TCDBi | 9.B.174.1.2, the two tunnel gated c-terminal processing protease (ctp) family |
Names & Taxonomyi
Protein namesi | Recommended name: Tail-specific protease (EC:3.4.21.102)Alternative name(s): C-terminal-processing peptidase PRC protein Protease Re |
Gene namesi | Name:prc Synonyms:tsp Ordered Locus Names:b1830, JW1819 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 397 | G → A: Loss of activity. Perturbs protein structure. 1 Publication | 1 | |
Mutagenesisi | 398 | G → A: Loss of activity. Perturbs protein structure. 1 Publication | 1 | |
Mutagenesisi | 452 | S → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 452 | S → C: Reduces activity by over 90%. 1 Publication | 1 | |
Mutagenesisi | 455 | E → A: Loss of activity. Perturbs protein structure. 1 Publication | 1 | |
Mutagenesisi | 463 | D → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 463 | D → N: Reduces activity by 90%. 1 Publication | 1 | |
Mutagenesisi | 474 | T → A: Loss of activity. Perturbs protein structure. 1 Publication | 1 | |
Mutagenesisi | 477 | K → A or H: Loss of activity. No apparent effect on protein structure. 1 Publication | 1 | |
Mutagenesisi | 477 | K → R: Loss of activity. Perturbs protein structure. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000027331 | 23 – 682 | Tail-specific proteaseAdd BLAST | 660 |
Proteomic databases
jPOSTi | P23865 |
PaxDbi | P23865 |
PRIDEi | P23865 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263463, 512 interactors |
DIPi | DIP-10557N |
IntActi | P23865, 3 interactors |
STRINGi | 511145.b1830 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23865 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 238 – 322 | PDZPROSITE-ProRule annotationAdd BLAST | 85 |
Sequence similaritiesi
Belongs to the peptidase S41A family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0793, Bacteria |
HOGENOMi | CLU_016199_1_0_6 |
InParanoidi | P23865 |
PhylomeDBi | P23865 |
Family and domain databases
CDDi | cd07560, Peptidase_S41_CPP, 1 hit |
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR001478, PDZ IPR036034, PDZ_sf IPR004447, Peptidase_S41A IPR005151, Tail-specific_protease IPR020992, Tail_Prtase_C IPR040573, TSP_N |
Pfami | View protein in Pfam PF11818, DUF3340, 1 hit PF00595, PDZ, 1 hit PF03572, Peptidase_S41, 1 hit PF17804, TSP_NTD, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit SM00245, TSPc, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR00225, prc, 1 hit |
PROSITEi | View protein in PROSITE PS50106, PDZ, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P23865-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNMFFRLTAL AGLLAIAGQT FAVEDITRAD QIPVLKEETQ HATVSERVTS
60 70 80 90 100
RFTRSHYRQF DLDQAFSAKI FDRYLNLLDY SHNVLLASDV EQFAKKKTEL
110 120 130 140 150
GDELRSGKLD VFYDLYNLAQ KRRFERYQYA LSVLEKPMDF TGNDTYNLDR
160 170 180 190 200
SKAPWPKNEA ELNALWDSKV KFDELSLKLT GKTDKEIRET LTRRYKFAIR
210 220 230 240 250
RLAQTNSEDV FSLAMTAFAR EIDPHTNYLS PRNTEQFNTE MSLSLEGIGA
260 270 280 290 300
VLQMDDDYTV INSMVAGGPA AKSKAISVGD KIVGVGQTGK PMVDVIGWRL
310 320 330 340 350
DDVVALIKGP KGSKVRLEIL PAGKGTKTRT VTLTRERIRL EDRAVKMSVK
360 370 380 390 400
TVGKEKVGVL DIPGFYVGLT DDVKVQLQKL EKQNVSSVII DLRSNGGGAL
410 420 430 440 450
TEAVSLSGLF IPAGPIVQVR DNNGKVREDS DTDGQVFYKG PLVVLVDRFS
460 470 480 490 500
ASASEIFAAA MQDYGRALVV GEPTFGKGTV QQYRSLNRIY DQMLRPEWPA
510 520 530 540 550
LGSVQYTIQK FYRVNGGSTQ RKGVTPDIIM PTGNEETETG EKFEDNALPW
560 570 580 590 600
DSIDAATYVK SGDLTAFEPE LLKEHNARIA KDPEFQNIMK DIARFNAMKD
610 620 630 640 650
KRNIVSLNYA VREKENNEDD ATRLARLNER FKREGKPELK KLDDLPKDYQ
660 670 680
EPDPYLDETV NIALDLAKLE KARPAEQPAP VK
Sequence cautioni
The sequence BAA00578 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 317 | L → Q in BAA00577 (PubMed:1856173).Curated | 1 | |
Sequence conflicti | 317 | L → Q in BAA00578 (PubMed:1856173).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00674 Genomic DNA Translation: BAA00577.1 D00674 Genomic DNA Translation: BAA00578.1 Different initiation. M75634 Genomic DNA Translation: AAA24699.1 U00096 Genomic DNA Translation: AAC74900.1 AP009048 Genomic DNA Translation: BAA15638.1 S49803 Genomic DNA Translation: AAB24313.1 L48409 Genomic DNA Translation: AAD41528.1 |
PIRi | A41798 |
RefSeqi | NP_416344.1, NC_000913.3 WP_001055791.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74900; AAC74900; b1830 BAA15638; BAA15638; BAA15638 |
GeneIDi | 58462260 946096 |
KEGGi | ecj:JW1819 eco:b1830 |
PATRICi | fig|511145.12.peg.1908 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00674 Genomic DNA Translation: BAA00577.1 D00674 Genomic DNA Translation: BAA00578.1 Different initiation. M75634 Genomic DNA Translation: AAA24699.1 U00096 Genomic DNA Translation: AAC74900.1 AP009048 Genomic DNA Translation: BAA15638.1 S49803 Genomic DNA Translation: AAB24313.1 L48409 Genomic DNA Translation: AAD41528.1 |
PIRi | A41798 |
RefSeqi | NP_416344.1, NC_000913.3 WP_001055791.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5WQL | X-ray | 2.30 | C/D | 1-682 | [»] | |
6IQQ | X-ray | 2.80 | C/D | 1-682 | [»] | |
6IQR | X-ray | 3.42 | A/B | 1-682 | [»] | |
6IQS | X-ray | 2.69 | C/D | 1-682 | [»] | |
6IQU | X-ray | 2.90 | B | 1-246 | [»] | |
B | 340-682 | [»] | ||||
SMRi | P23865 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263463, 512 interactors |
DIPi | DIP-10557N |
IntActi | P23865, 3 interactors |
STRINGi | 511145.b1830 |
Protein family/group databases
MEROPSi | S41.001 |
TCDBi | 9.B.174.1.2, the two tunnel gated c-terminal processing protease (ctp) family |
Proteomic databases
jPOSTi | P23865 |
PaxDbi | P23865 |
PRIDEi | P23865 |
Genome annotation databases
EnsemblBacteriai | AAC74900; AAC74900; b1830 BAA15638; BAA15638; BAA15638 |
GeneIDi | 58462260 946096 |
KEGGi | ecj:JW1819 eco:b1830 |
PATRICi | fig|511145.12.peg.1908 |
Organism-specific databases
EchoBASEi | EB0753 |
Phylogenomic databases
eggNOGi | COG0793, Bacteria |
HOGENOMi | CLU_016199_1_0_6 |
InParanoidi | P23865 |
PhylomeDBi | P23865 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10760-MONOMER MetaCyc:EG10760-MONOMER |
BRENDAi | 3.4.21.102, 2026 |
Miscellaneous databases
PROi | PR:P23865 |
Family and domain databases
CDDi | cd07560, Peptidase_S41_CPP, 1 hit |
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR001478, PDZ IPR036034, PDZ_sf IPR004447, Peptidase_S41A IPR005151, Tail-specific_protease IPR020992, Tail_Prtase_C IPR040573, TSP_N |
Pfami | View protein in Pfam PF11818, DUF3340, 1 hit PF00595, PDZ, 1 hit PF03572, Peptidase_S41, 1 hit PF17804, TSP_NTD, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit SM00245, TSPc, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR00225, prc, 1 hit |
PROSITEi | View protein in PROSITE PS50106, PDZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRC_ECOLI | |
Accessioni | P23865Primary (citable) accession number: P23865 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families