UniProtKB - P23845 (CYSN_ECOLI)
Protein
Sulfate adenylyltransferase subunit 1
Gene
cysN
Organism
Escherichia coli (strain K12)
Status
Functioni
May be the GTPase, regulating ATP sulfurylase activity.
Catalytic activityi
- EC:2.7.7.4
Activity regulationi
GTPase activity is coupled to stimulation of the rate of APS formation.
: hydrogen sulfide biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD), Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
- Adenylyl-sulfate kinase (cysC), Adenylyl-sulfate kinase (cysC)
- Phosphoadenosine phosphosulfate reductase (cysH), Phosphoadenosine phosphosulfate reductase (cysH)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 34 – 41 | GTPBy similarity | 8 | |
Nucleotide bindingi | 113 – 117 | GTPBy similarity | 5 | |
Nucleotide bindingi | 168 – 171 | GTPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- GTPase activity Source: EcoliWiki
- GTP binding Source: EcoliWiki
- sulfate adenylyltransferase (ATP) activity Source: EcoCyc
GO - Biological processi
- hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
- sulfate assimilation Source: UniProtKB-UniRule
- sulfur compound metabolic process Source: EcoliWiki
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Ligand | ATP-binding, GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:CYSN-MONOMER MetaCyc:CYSN-MONOMER |
SABIO-RKi | P23845 |
UniPathwayi | UPA00140;UER00204 |
Names & Taxonomyi
Protein namesi | Recommended name: Sulfate adenylyltransferase subunit 1 (EC:2.7.7.4)Alternative name(s): ATP-sulfurylase large subunit Sulfate adenylate transferase Short name: SAT |
Gene namesi | Name:cysN Ordered Locus Names:b2751, JW2721 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091522 | 1 – 475 | Sulfate adenylyltransferase subunit 1Add BLAST | 475 |
Proteomic databases
jPOSTi | P23845 |
PaxDbi | P23845 |
PRIDEi | P23845 |
Interactioni
Subunit structurei
Heterodimer composed of CysD, the smaller subunit, and CysN.
Binary interactionsi
P23845
With | #Exp. | IntAct |
---|---|---|
cysD [P21156] | 2 | EBI-559728,EBI-1130200 |
Protein-protein interaction databases
BioGRIDi | 4262932, 28 interactors 851551, 1 interactor |
ComplexPortali | CPX-4471, Sulfate adenylyltransferase complex |
DIPi | DIP-513N |
IntActi | P23845, 3 interactors |
STRINGi | 511145.b2751 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 239 | tr-type GAdd BLAST | 215 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 41 | G1By similarity | 8 | |
Regioni | 92 – 96 | G2By similarity | 5 | |
Regioni | 113 – 116 | G3By similarity | 4 | |
Regioni | 168 – 171 | G4By similarity | 4 | |
Regioni | 206 – 208 | G5By similarity | 3 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.Curated
Phylogenomic databases
eggNOGi | COG2895, Bacteria |
HOGENOMi | CLU_007265_5_2_6 |
InParanoidi | P23845 |
PhylomeDBi | P23845 |
Family and domain databases
CDDi | cd04166, CysN_ATPS, 1 hit cd03695, CysN_NodQ_II, 1 hit cd04095, CysN_NoDQ_III, 1 hit |
HAMAPi | MF_00062, Sulf_adenylyltr_sub1, 1 hit |
InterProi | View protein in InterPro IPR041757, CysN_GTP-bd IPR044138, CysN_II IPR044139, CysN_NoDQ_III IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR011779, SO4_adenylTrfase_lsu IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR02034, CysN, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P23845-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNTALAQQIA NEGGVEAWMI AQQHKSLLRF LTCGSVDDGK STLIGRLLHD
60 70 80 90 100
TRQIYEDQLS SLHNDSKRHG TQGEKLDLAL LVDGLQAERE QGITIDVAYR
110 120 130 140 150
YFSTEKRKFI IADTPGHEQY TRNMATGAST CELAILLIDA RKGVLDQTRR
160 170 180 190 200
HSFISTLLGI KHLVVAINKM DLVDYSEETF TRIREDYLTF AGQLPGNLDI
210 220 230 240 250
RFVPLSALEG DNVASQSESM PWYSGPTLLE VLETVEIQRV VDAQPMRFPV
260 270 280 290 300
QYVNRPNLDF RGYAGTLASG RVEVGQRVKV LPSGVESNVA RIVTFDGDRE
310 320 330 340 350
EAFAGEAITL VLTDEIDISR GDLLLAADEA LPAVQSASVD VVWMAEQPLS
360 370 380 390 400
PGQSYDIKIA GKKTRARVDG IRYQVDINNL TQREVENLPL NGIGLVDLTF
410 420 430 440 450
DEPLVLDRYQ QNPVTGGLIF IDRLSNVTVG AGMVHEPVSQ ATAAPSEFSA
460 470
FELELNALVR RHFPHWGARD LLGDK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74586 Genomic DNA Translation: AAA23646.1 U29579 Genomic DNA Translation: AAA69261.1 U00096 Genomic DNA Translation: AAC75793.1 AP009048 Genomic DNA Translation: BAE76828.1 |
PIRi | JN0327 |
RefSeqi | NP_417231.1, NC_000913.3 WP_001090361.1, NZ_SSZK01000017.1 |
Genome annotation databases
EnsemblBacteriai | AAC75793; AAC75793; b2751 BAE76828; BAE76828; BAE76828 |
GeneIDi | 947219 |
KEGGi | ecj:JW2721 eco:b2751 |
PATRICi | fig|1411691.4.peg.3989 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74586 Genomic DNA Translation: AAA23646.1 U29579 Genomic DNA Translation: AAA69261.1 U00096 Genomic DNA Translation: AAC75793.1 AP009048 Genomic DNA Translation: BAE76828.1 |
PIRi | JN0327 |
RefSeqi | NP_417231.1, NC_000913.3 WP_001090361.1, NZ_SSZK01000017.1 |
3D structure databases
SMRi | P23845 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262932, 28 interactors 851551, 1 interactor |
ComplexPortali | CPX-4471, Sulfate adenylyltransferase complex |
DIPi | DIP-513N |
IntActi | P23845, 3 interactors |
STRINGi | 511145.b2751 |
Proteomic databases
jPOSTi | P23845 |
PaxDbi | P23845 |
PRIDEi | P23845 |
Genome annotation databases
EnsemblBacteriai | AAC75793; AAC75793; b2751 BAE76828; BAE76828; BAE76828 |
GeneIDi | 947219 |
KEGGi | ecj:JW2721 eco:b2751 |
PATRICi | fig|1411691.4.peg.3989 |
Organism-specific databases
EchoBASEi | EB0191 |
Phylogenomic databases
eggNOGi | COG2895, Bacteria |
HOGENOMi | CLU_007265_5_2_6 |
InParanoidi | P23845 |
PhylomeDBi | P23845 |
Enzyme and pathway databases
UniPathwayi | UPA00140;UER00204 |
BioCyci | EcoCyc:CYSN-MONOMER MetaCyc:CYSN-MONOMER |
SABIO-RKi | P23845 |
Miscellaneous databases
PROi | PR:P23845 |
Family and domain databases
CDDi | cd04166, CysN_ATPS, 1 hit cd03695, CysN_NodQ_II, 1 hit cd04095, CysN_NoDQ_III, 1 hit |
HAMAPi | MF_00062, Sulf_adenylyltr_sub1, 1 hit |
InterProi | View protein in InterPro IPR041757, CysN_GTP-bd IPR044138, CysN_II IPR044139, CysN_NoDQ_III IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR011779, SO4_adenylTrfase_lsu IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR02034, CysN, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYSN_ECOLI | |
Accessioni | P23845Primary (citable) accession number: P23845 Secondary accession number(s): Q2MA78 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | February 10, 2021 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families