UniProtKB - P23836 (PHOP_ECOLI)
Protein
Transcriptional regulatory protein PhoP
Gene
phoP
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of the two-component regulatory system PhoP/PhoQ involved in adaptation to low Mg2+ environments and the control of acid resistance genes. In low periplasmic Mg2+, PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg2+, PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG (By similarity). Mediates magnesium influx to the cytosol by activation of MgtA. Promotes expression of the two-component regulatory system rstA/rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes.By similarity1 Publication
Miscellaneous
There is a close linkage between the Rcs and PhoQ/P signaling systems, and both signaling systems respond to certain external divalent cations (zinc and magnesium).
Two-component regulatory system EvgA/EvgS interacts with PhoP/PhoQ via signal transduction mediated by phospho-EvgA.
Activity regulationi
Feedback inhibited by MgrB, which seems to bind PhoQ, altering its activity and that of downstream effector PhoP. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, or treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 124 – 222 | OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST | 99 |
GO - Molecular functioni
- DNA-binding transcription activator activity Source: CollecTF
- identical protein binding Source: IntAct
- phosphorelay response regulator activity Source: GO_Central
- transcription regulatory region sequence-specific DNA binding Source: CollecTF
GO - Biological processi
- positive regulation of transcription, DNA-templated Source: CollecTF
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation, Two-component regulatory system |
Enzyme and pathway databases
BioCyci | EcoCyc:PHOP-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional regulatory protein PhoP |
Gene namesi | Name:phoP Ordered Locus Names:b1130, JW1116 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Cytosol
- cytosol Source: EcoCyc
Other locations
- protein-DNA complex Source: CollecTF
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Altered expression of 33 genes; 9 are down-regulated, the rest up-regulated.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081195 | 1 – 223 | Transcriptional regulatory protein PhoPAdd BLAST | 223 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 51 | 4-aspartylphosphatePROSITE-ProRule annotation | 1 |
Post-translational modificationi
Phosphorylated by PhoQ.Curated
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P23836 |
PaxDbi | P23836 |
PRIDEi | P23836 |
PTM databases
iPTMneti | P23836 |
Expressioni
Inductioni
The phoP/phoQ operon is positively autoregulated by both PhoP and PhoQ in a Mg2+-dependent manner, inhibited at high Mg2+ concentrations (PubMed:10464230). Induced by dsbA disruption and dithiothreitol (PubMed:22267510).2 Publications
Gene expression databases
CollecTFi | EXPREG_00000950 |
Interactioni
Subunit structurei
Monomer in the inactive, unphosphorylated state and dimer in the active, phosphorylated state.
1 PublicationBinary interactionsi
P23836
With | #Exp. | IntAct |
---|---|---|
itself | 5 | EBI-1121453,EBI-1121453 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4260094, 19 interactors 850069, 1 interactor |
DIPi | DIP-10500N |
IntActi | P23836, 6 interactors |
STRINGi | 511145.b1130 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23836 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23836 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 116 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 115 |
Phylogenomic databases
eggNOGi | COG0745, Bacteria |
HOGENOMi | CLU_000445_30_1_6 |
InParanoidi | P23836 |
PhylomeDBi | P23836 |
Family and domain databases
CDDi | cd00383, trans_reg_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR001867, OmpR/PhoB-type_DNA-bd IPR001789, Sig_transdc_resp-reg_receiver IPR039420, WalR-like IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR48111, PTHR48111, 1 hit |
Pfami | View protein in Pfam PF00072, Response_reg, 1 hit PF00486, Trans_reg_C, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit SM00862, Trans_reg_C, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS51755, OMPR_PHOB, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
P23836-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRVLVVEDNA LLRHHLKVQI QDAGHQVDDA EDAKEADYYL NEHIPDIAIV
60 70 80 90 100
DLGLPDEDGL SLIRRWRSND VSLPILVLTA RESWQDKVEV LSAGADDYVT
110 120 130 140 150
KPFHIEEVMA RMQALMRRNS GLASQVISLP PFQVDLSRRE LSINDEVIKL
160 170 180 190 200
TAFEYTIMET LIRNNGKVVS KDSLMLQLYP DAELRESHTI DVLMGRLRKK
210 220
IQAQYPQEVI TTVRGQGYLF ELR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D90393 Genomic DNA Translation: BAA14390.1 M81433 Genomic DNA Translation: AAA24377.1 U00096 Genomic DNA Translation: AAC74214.1 AP009048 Genomic DNA Translation: BAA35952.1 M74924 Genomic DNA Translation: AAA92732.1 X59307 Genomic DNA Translation: CAA41997.1 |
PIRi | A41965 |
RefSeqi | NP_415648.1, NC_000913.3 WP_001265471.1, NZ_STEB01000016.1 |
Genome annotation databases
EnsemblBacteriai | AAC74214; AAC74214; b1130 BAA35952; BAA35952; BAA35952 |
GeneIDi | 57731916 945697 |
KEGGi | ecj:JW1116 eco:b1130 |
PATRICi | fig|1411691.4.peg.1136 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D90393 Genomic DNA Translation: BAA14390.1 M81433 Genomic DNA Translation: AAA24377.1 U00096 Genomic DNA Translation: AAC74214.1 AP009048 Genomic DNA Translation: BAA35952.1 M74924 Genomic DNA Translation: AAA92732.1 X59307 Genomic DNA Translation: CAA41997.1 |
PIRi | A41965 |
RefSeqi | NP_415648.1, NC_000913.3 WP_001265471.1, NZ_STEB01000016.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2PKX | X-ray | 2.54 | A/B | 1-121 | [»] | |
2PL1 | X-ray | 1.90 | A | 1-121 | [»] | |
SMRi | P23836 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260094, 19 interactors 850069, 1 interactor |
DIPi | DIP-10500N |
IntActi | P23836, 6 interactors |
STRINGi | 511145.b1130 |
PTM databases
iPTMneti | P23836 |
Proteomic databases
jPOSTi | P23836 |
PaxDbi | P23836 |
PRIDEi | P23836 |
Genome annotation databases
EnsemblBacteriai | AAC74214; AAC74214; b1130 BAA35952; BAA35952; BAA35952 |
GeneIDi | 57731916 945697 |
KEGGi | ecj:JW1116 eco:b1130 |
PATRICi | fig|1411691.4.peg.1136 |
Organism-specific databases
EchoBASEi | EB0724 |
Phylogenomic databases
eggNOGi | COG0745, Bacteria |
HOGENOMi | CLU_000445_30_1_6 |
InParanoidi | P23836 |
PhylomeDBi | P23836 |
Enzyme and pathway databases
BioCyci | EcoCyc:PHOP-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P23836 |
PROi | PR:P23836 |
Gene expression databases
CollecTFi | EXPREG_00000950 |
Family and domain databases
CDDi | cd00383, trans_reg_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR001867, OmpR/PhoB-type_DNA-bd IPR001789, Sig_transdc_resp-reg_receiver IPR039420, WalR-like IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR48111, PTHR48111, 1 hit |
Pfami | View protein in Pfam PF00072, Response_reg, 1 hit PF00486, Trans_reg_C, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit SM00862, Trans_reg_C, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS51755, OMPR_PHOB, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHOP_ECOLI | |
Accessioni | P23836Primary (citable) accession number: P23836 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | April 7, 2021 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references