UniProtKB - P23818 (GRIA1_MOUSE)
Glutamate receptor 1
Gria1
Functioni
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
Miscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 464 | GlutamateBy similarity | 1 | |
Binding sitei | 499 | GlutamateBy similarity | 1 | |
Binding sitei | 719 | GlutamateBy similarity | 1 |
GO - Molecular functioni
- adenylate cyclase binding Source: MGI
- AMPA glutamate receptor activity Source: MGI
- amyloid-beta binding Source: MGI
- beta-2 adrenergic receptor binding Source: MGI
- glutamate receptor binding Source: MGI
- G-protein alpha-subunit binding Source: MGI
- G-protein beta-subunit binding Source: MGI
- identical protein binding Source: MGI
- immunoglobulin binding Source: MGI
- ionotropic glutamate receptor activity Source: MGI
- ligand-gated ion channel activity Source: GO_Central
- myosin V binding Source: MGI
- neurotransmitter receptor activity involved in regulation of postsynaptic cytosolic calcium ion concentration Source: SynGO
- PDZ domain binding Source: MGI
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein kinase A binding Source: MGI
- protein kinase binding Source: MGI
- scaffold protein binding Source: MGI
- signaling receptor activity Source: GO_Central
- small GTPase binding Source: MGI
- transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential Source: SynGO
GO - Biological processi
- cellular response to ammonium ion Source: MGI
- chemical synaptic transmission Source: MGI
- long-term memory Source: UniProtKB
- long-term synaptic depression Source: MGI
- modulation of chemical synaptic transmission Source: MGI
- neuronal action potential Source: MGI
- positive regulation of membrane potential Source: MGI
- positive regulation of synaptic transmission Source: MGI
- receptor internalization Source: UniProtKB
- regulation of postsynaptic membrane potential Source: SynGO
- regulation of receptor recycling Source: MGI
- regulation of synaptic plasticity Source: MGI
- response to toxic substance Source: MGI
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-204005, COPII-mediated vesicle transport R-MMU-399710, Activation of AMPA receptors R-MMU-416993, Trafficking of GluR2-containing AMPA receptors R-MMU-438066, Unblocking of NMDA receptors, glutamate binding and activation R-MMU-5694530, Cargo concentration in the ER R-MMU-8849932, Synaptic adhesion-like molecules |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate receptor 1Short name: GluR-1 Alternative name(s): AMPA-selective glutamate receptor 1 GluR-A GluR-K1 Glutamate receptor ionotropic, AMPA 1 Short name: GluA1 |
Gene namesi | Name:Gria1 Synonyms:Glur1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95808, Gria1 |
VEuPathDBi | HostDB:ENSMUSG00000020524 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
- postsynaptic cell membrane 3 Publications; Multi-pass membrane protein 1 Publication
- postsynaptic density membrane 1 Publication; Multi-pass membrane protein Curated
Endosome
- Early endosome membrane By similarity; Multi-pass membrane protein By similarity
- Recycling endosome membrane By similarity; Multi-pass membrane protein By similarity
Other locations
- dendrite 1 Publication
- dendritic spine 1 Publication
- presynapse 1 Publication
- synapse 1 Publication
Note: Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. Colocalizes with PDLIM4 in early endosomes. Displays a somatodendritic localization and is excluded from axons in neurons (PubMed:18341993). Localized to cone photoreceptor pedicles (PubMed:28334377).By similarity2 Publications
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: UniProtKB
Endosome
- early endosome Source: MGI
- early endosome membrane Source: UniProtKB
- recycling endosome Source: MGI
- recycling endosome membrane Source: UniProtKB
Extracellular region or secreted
- perisynaptic space Source: MGI
Plasma Membrane
- AMPA glutamate receptor complex Source: MGI
- dendrite membrane Source: BHF-UCL
- dendritic spine membrane Source: MGI
- integral component of postsynaptic density membrane Source: SynGO
- integral component of postsynaptic membrane Source: MGI
- integral component of presynaptic membrane Source: MGI
- ionotropic glutamate receptor complex Source: BHF-UCL
- plasma membrane Source: BHF-UCL
- postsynaptic density membrane Source: SynGO
- postsynaptic membrane Source: BHF-UCL
- synaptic membrane Source: UniProtKB
Other locations
- asymmetric synapse Source: MGI
- axonal spine Source: MGI
- cell body Source: MGI
- cell surface Source: BHF-UCL
- cell-cell junction Source: MGI
- dendrite Source: UniProtKB
- dendritic shaft Source: MGI
- dendritic spine Source: UniProtKB
- excitatory synapse Source: MGI
- glutamatergic synapse Source: MGI
- membrane Source: MGI
- neuromuscular junction Source: MGI
- neuron projection Source: BHF-UCL
- neuron spine Source: BHF-UCL
- neuronal cell body Source: BHF-UCL
- postsynapse Source: UniProtKB
- postsynaptic density Source: UniProtKB
- presynapse Source: UniProtKB
- protein-containing complex Source: MGI
- somatodendritic compartment Source: UniProtKB
- synapse Source: UniProtKB
- synaptic vesicle Source: MGI
- synaptic vesicle membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 19 – 536 | ExtracellularBy similarityAdd BLAST | 518 | |
Transmembranei | 537 – 557 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 558 – 584 | CytoplasmicBy similarityAdd BLAST | 27 | |
Intramembranei | 585 – 600 | Helical; Pore-formingBy similarityAdd BLAST | 16 | |
Intramembranei | 601 – 603 | By similarity | 3 | |
Topological domaini | 604 – 609 | CytoplasmicBy similarity | 6 | |
Transmembranei | 610 – 630 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 631 – 805 | ExtracellularBy similarityAdd BLAST | 175 | |
Transmembranei | 806 – 826 | Helical; Name=M4By similarityAdd BLAST | 21 | |
Topological domaini | 827 – 907 | CytoplasmicBy similarityAdd BLAST | 81 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Endoplasmic reticulum, Endosome, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 905 – 907 | TGL → AGA: Almost abolishes interaction with SHANK3. 1 Publication | 3 |
Chemistry databases
ChEMBLi | CHEMBL3502 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000011530 | 19 – 907 | Glutamate receptor 1Add BLAST | 889 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 63 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 75 ↔ 323 | By similarity | ||
Glycosylationi | 249 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 257 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 363 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 401 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 406 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 603 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 645 | PhosphoserineBy similarity | 1 | |
Modified residuei | 710 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 732 ↔ 787 | By similarity | ||
Lipidationi | 829 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 849 | PhosphoserineBy similarity | 1 | |
Modified residuei | 863 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
MaxQBi | P23818 |
PaxDbi | P23818 |
PRIDEi | P23818 |
ProteomicsDBi | 271017 |
PTM databases
GlyConnecti | 2341, 4 N-Linked glycans (3 sites) |
GlyGeni | P23818, 6 sites, 4 N-linked glycans (3 sites) |
iPTMneti | P23818 |
PhosphoSitePlusi | P23818 |
SwissPalmi | P23818 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000020524, Expressed in dentate gyrus of hippocampal formation and 203 other tissues |
ExpressionAtlasi | P23818, baseline and differential |
Genevisiblei | P23818, MM |
Interactioni
Subunit structurei
Homotetramer or heterotetramer of pore-forming glutamate receptor subunits (By similarity). Tetramers may be formed by the dimerization of dimers (By similarity).
Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8 (By similarity).
Interacts with HIP1 and RASGRF2 (PubMed:12839988, PubMed:16407208).
Interacts with SYNDIG1 and GRIA2 (PubMed:20152115).
Interacts with DLG1 (via C-terminus).
Interacts with LRFN1.
Interacts with PRKG2.
Interacts with CNIH2 and CACNG2.
Interacts with CACNG5.
Interacts (via C-terminus) with PDLIM4 (via LIM domain); this interaction as well as the interaction of PDLIM4 with alpha-actinin is required for their colocalization in early endosomes.
Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8 (By similarity).
Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes (PubMed:23524343).
Interacts (via PDZ-binding motif) with SHANK3 (via PDZ domain) (PubMed:16606358).
Interacts with CACNG3; associates GRIA1 with the adapter protein complex 4 (AP-4) to target GRIA1 to the somatodendritic compartment of neurons (PubMed:18341993).
By similarity6 PublicationsBinary interactionsi
GO - Molecular functioni
- adenylate cyclase binding Source: MGI
- beta-2 adrenergic receptor binding Source: MGI
- glutamate receptor binding Source: MGI
- G-protein alpha-subunit binding Source: MGI
- G-protein beta-subunit binding Source: MGI
- identical protein binding Source: MGI
- myosin V binding Source: MGI
- PDZ domain binding Source: MGI
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein kinase A binding Source: MGI
- protein kinase binding Source: MGI
- scaffold protein binding Source: MGI
- small GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 200058, 21 interactors |
DIPi | DIP-31970N |
IntActi | P23818, 11 interactors |
MINTi | P23818 |
STRINGi | 10090.ENSMUSP00000044494 |
Chemistry databases
BindingDBi | P23818 |
Miscellaneous databases
RNActi | P23818, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 492 – 494 | Glutamate bindingBy similarity | 3 | |
Regioni | 668 – 669 | Glutamate bindingBy similarity | 2 | |
Regioni | 857 – 881 | DisorderedSequence analysisAdd BLAST | 25 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 904 – 907 | PDZ-binding | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1054, Eukaryota |
GeneTreei | ENSGT00940000157342 |
InParanoidi | P23818 |
PhylomeDBi | P23818 |
TreeFami | TF315232 |
Family and domain databases
InterProi | View protein in InterPro IPR001828, ANF_lig-bd_rcpt IPR019594, Glu/Gly-bd IPR001508, Iono_rcpt_met IPR001320, Iontro_rcpt IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF01094, ANF_receptor, 1 hit PF00060, Lig_chan, 1 hit PF10613, Lig_chan-Glu_bd, 1 hit |
PRINTSi | PR00177, NMDARECEPTOR |
SMARTi | View protein in SMART SM00918, Lig_chan-Glu_bd, 1 hit SM00079, PBPe, 1 hit |
SUPFAMi | SSF53822, SSF53822, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MPYIFAFFCT GFLGAVVGAN FPNNIQIGGL FPNQQSQEHA AFRFALSQLT
60 70 80 90 100
EPPKLLPQID IVNISDSFEM TYRFCSQFSK GVYAIFGFYE RRTVNMLTSF
110 120 130 140 150
CGALHVCFIT PSFPVDTSNQ FVLQLRPELQ EALISIIDHY KWQTFVYIYD
160 170 180 190 200
ADRGLSVLQR VLDTAAEKNW QVTAVNILTT TEEGYRMLFQ DLEKKKERLV
210 220 230 240 250
VVDCESERLN AILGQIVKLE KNGIGYHYIL ANLGFMDIDL NKFKESGANV
260 270 280 290 300
TGFQLVNYTD TIPARIMQQW RTSDARDHTR VDWKRPKYTS ALTYDGVKVM
310 320 330 340 350
AEAFQSLRRQ RIDISRRGNA GDCLANPAVP WGQGIDIQRA LQQVRFEGLT
360 370 380 390 400
GNVQFNEKGR RTNYTLHVIE MKHDGIRKIG YWNEDDKFVP AATDAQAGGD
410 420 430 440 450
NSSVQNRTYI VTTILEDPYV MLKKNANQFE GNDRYEGYCV ELAAEIAKHV
460 470 480 490 500
GYSYRLEIVS DGKYGARDPD TKAWNGMVGE LVYGRADVAV APLTITLVRE
510 520 530 540 550
EVIDFSKPFM SLGISIMIKK PQKSKPGVFS FLDPLAYEIW MCIVFAYIGV
560 570 580 590 600
SVVLFLVSRF SPYEWHSEEF EEGRDQTTSD QSNEFGIFNS LWFSLGAFMQ
610 620 630 640 650
QGCDISPRSL SGRIVGGVWW FFTLIIISSY TANLAAFLTV ERMVSPIESA
660 670 680 690 700
EDLAKQTEIA YGTLEAGSTK EFFRRSKIAV FEKMWTYMKS AEPSVFVRTT
710 720 730 740 750
EEGMIRVRKS KGKYAYLLES TMNEYIEQRK PCDTMKVGGN LDSKGYGIAT
760 770 780 790 800
PKGSALRGPV NLAVLKLSEQ GVLDKLKSKW WYDKGECGSK DSGSKDKTSA
810 820 830 840 850
LSLSNVAGVF YILIGGLGLA MLVALIEFCY KSRSESKRMK GFCLIPQQSI
860 870 880 890 900
NEAIRTSTLP RNSGAGASGG SGSGENGRVV SQDFPKSMQS IPCMSHSSGM
PLGATGL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ7TNB5 | Q7TNB5_MOUSE | Glutamate receptor | Gria1 | 907 | Annotation score: | ||
F6YNQ1 | F6YNQ1_MOUSE | Glutamate receptor | Gria1 | 838 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57497 mRNA Translation: CAA40734.1 |
CCDSi | CCDS48800.1 |
PIRi | S12874 |
RefSeqi | NP_001106796.1, NM_001113325.2 |
Genome annotation databases
Ensembli | ENSMUST00000036315; ENSMUSP00000044494; ENSMUSG00000020524 |
GeneIDi | 14799 |
KEGGi | mmu:14799 |
UCSCi | uc007izs.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57497 mRNA Translation: CAA40734.1 |
CCDSi | CCDS48800.1 |
PIRi | S12874 |
RefSeqi | NP_001106796.1, NM_001113325.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7LDD | electron microscopy | 3.40 | A/C | 1-907 | [»] | |
7LDE | electron microscopy | 3.90 | A/C | 1-907 | [»] | |
SMRi | P23818 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200058, 21 interactors |
DIPi | DIP-31970N |
IntActi | P23818, 11 interactors |
MINTi | P23818 |
STRINGi | 10090.ENSMUSP00000044494 |
Chemistry databases
BindingDBi | P23818 |
ChEMBLi | CHEMBL3502 |
PTM databases
GlyConnecti | 2341, 4 N-Linked glycans (3 sites) |
GlyGeni | P23818, 6 sites, 4 N-linked glycans (3 sites) |
iPTMneti | P23818 |
PhosphoSitePlusi | P23818 |
SwissPalmi | P23818 |
Proteomic databases
MaxQBi | P23818 |
PaxDbi | P23818 |
PRIDEi | P23818 |
ProteomicsDBi | 271017 |
Protocols and materials databases
ABCDi | P23818, 1 sequenced antibody |
Antibodypediai | 28276, 1030 antibodies from 46 providers |
DNASUi | 14799 |
Genome annotation databases
Ensembli | ENSMUST00000036315; ENSMUSP00000044494; ENSMUSG00000020524 |
GeneIDi | 14799 |
KEGGi | mmu:14799 |
UCSCi | uc007izs.3, mouse |
Organism-specific databases
CTDi | 2890 |
MGIi | MGI:95808, Gria1 |
VEuPathDBi | HostDB:ENSMUSG00000020524 |
Phylogenomic databases
eggNOGi | KOG1054, Eukaryota |
GeneTreei | ENSGT00940000157342 |
InParanoidi | P23818 |
PhylomeDBi | P23818 |
TreeFami | TF315232 |
Enzyme and pathway databases
Reactomei | R-MMU-204005, COPII-mediated vesicle transport R-MMU-399710, Activation of AMPA receptors R-MMU-416993, Trafficking of GluR2-containing AMPA receptors R-MMU-438066, Unblocking of NMDA receptors, glutamate binding and activation R-MMU-5694530, Cargo concentration in the ER R-MMU-8849932, Synaptic adhesion-like molecules |
Miscellaneous databases
BioGRID-ORCSi | 14799, 0 hits in 65 CRISPR screens |
ChiTaRSi | Gria1, mouse |
PROi | PR:P23818 |
RNActi | P23818, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020524, Expressed in dentate gyrus of hippocampal formation and 203 other tissues |
ExpressionAtlasi | P23818, baseline and differential |
Genevisiblei | P23818, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001828, ANF_lig-bd_rcpt IPR019594, Glu/Gly-bd IPR001508, Iono_rcpt_met IPR001320, Iontro_rcpt IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF01094, ANF_receptor, 1 hit PF00060, Lig_chan, 1 hit PF10613, Lig_chan-Glu_bd, 1 hit |
PRINTSi | PR00177, NMDARECEPTOR |
SMARTi | View protein in SMART SM00918, Lig_chan-Glu_bd, 1 hit SM00079, PBPe, 1 hit |
SUPFAMi | SSF53822, SSF53822, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GRIA1_MOUSE | |
Accessioni | P23818Primary (citable) accession number: P23818 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | February 23, 2022 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families