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Protein

Acetylcholinesterase

Gene

ACHE

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

Catalytic activityi

Acetylcholine + H2O = choline + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei233Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei364Charge relay systemBy similarity1
Active sitei477Charge relay systemBy similarity1

GO - Molecular functioni

  • acetylcholinesterase activity Source: UniProtKB-EC
  • amyloid-beta binding Source: HGNC

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase
Biological processNeurotransmitter degradation

Enzyme and pathway databases

BRENDAi3.1.1.7 908

Protein family/group databases

ESTHERibovin-ACHE AChE
MEROPSiS09.979

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholinesterase (EC:3.1.1.7)
Short name:
AChE
Gene namesi
Name:ACHE
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Secreted, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4768

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Add BLAST30
ChainiPRO_000000858531 – 613AcetylcholinesteraseAdd BLAST583

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi91N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi99 ↔ 126By similarity
Disulfide bondi287 ↔ 302By similarity
Glycosylationi295N-linked (GlcNAc...) asparagineCurated1
Glycosylationi380N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi439 ↔ 559By similarity
Glycosylationi494N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi610InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP23795
PRIDEiP23795

PTM databases

GlyConnecti8
UniCarbKBiP23795

Interactioni

Subunit structurei

Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers (By similarity). Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001517

Chemistry databases

BindingDBiP23795

Structurei

3D structure databases

ProteinModelPortaliP23795
SMRiP23795
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4389 Eukaryota
COG2272 LUCA
HOGENOMiHOG000091866
HOVERGENiHBG008839
InParanoidiP23795
KOiK01049

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR014788 AChE_tetra
IPR002018 CarbesteraseB
IPR019826 Carboxylesterase_B_AS
IPR019819 Carboxylesterase_B_CS
IPR000997 Cholinesterase
PfamiView protein in Pfam
PF08674 AChE_tetra, 1 hit
PF00135 COesterase, 1 hit
PRINTSiPR00878 CHOLNESTRASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD415333 AChE_tetra, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00122 CARBOXYLESTERASE_B_1, 1 hit
PS00941 CARBOXYLESTERASE_B_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform T (identifier: P23795-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRPPWCPLHT PSLTPPLLLL LFLIGGGAEA EGPEDPELLV MVRGGRLRGL
60 70 80 90 100
RLMAPRGPVS AFLGIPFAEP PVGPRRFLPP EPKRPWPGVL NATAFQSVCY
110 120 130 140 150
QYVDTLYPGF EGTEMWNPNR ELSEDCLYLN VWTPYPRPSS PTPVLVWIYG
160 170 180 190 200
GGFYSGASSL DVYDGRFLTQ AEGTVLVSMN YRVGAFGFLA LPGSREAPGN
210 220 230 240 250
VGLLDQRLAL QWVQENVAAF GGDPTSVTLF GESAGAASVG MHLLSPPSRG
260 270 280 290 300
LFHRAVLQSG APNGPWATVG VGEARRRATL LARLVGCPPG GAGGNDTELV
310 320 330 340 350
ACLRARPAQD LVDHEWRVLP QESVFRFSFV PVVDGDFLSD TPEALINAGD
360 370 380 390 400
FHGLQVLVGV VKDEGSYFLV YGAPGFSKDN ESLISRAQFL AGVRVGVPQA
410 420 430 440 450
SDLAAEAVVL HYTDWLHPED PARLREALSD VVGDHNVVCP VAQLAGRLAA
460 470 480 490 500
QGARVYAYIF EHRASTLSWP LWMGVPHGYE IEFIFGLPLE PSLNYTIEER
510 520 530 540 550
TFAQRLMRYW ANFARTGDPN DPRDPKAPQW PPYTAGAQQY VSLNLRPLEV
560 570 580 590 600
RRGLRAQACA FWNRFLPKLL SATDTLDEAE RQWKAEFHRW SSYMVHWKNQ
610
FDHYSKQDRC SDL
Length:613
Mass (Da):67,664
Last modified:July 11, 2001 - v2
Checksum:i698D4F0DF8624B12
GO
Isoform H (identifier: P23795-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     574-613: DTLDEAERQW...YSKQDRCSDL → ASEAPCTCSG...LFLLSRLLRL

Show »
Length:614
Mass (Da):66,908
Checksum:i444F4D6D0EDFDAE3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24I → L in AAI23899 (Ref. 2) Curated1
Sequence conflicti46R → E AA sequence (PubMed:2365060).Curated1
Sequence conflicti169T → V AA sequence (PubMed:2365060).Curated1
Sequence conflicti212W → S AA sequence (PubMed:2365060).Curated1
Sequence conflicti323S → H AA sequence (PubMed:2365060).Curated1
Sequence conflicti352H → V AA sequence (PubMed:2365060).Curated1
Sequence conflicti424L → W AA sequence (PubMed:2365060).Curated1
Sequence conflicti524D → A AA sequence (PubMed:2365060).Curated1
Sequence conflicti549 – 554EVRRGL → GVPQAS AA sequence (PubMed:2365060).Curated6
Sequence conflicti571S → N AA sequence (PubMed:2365060).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001455574 – 613DTLDE…RCSDL → ASEAPCTCSGPAHGEAAPRP RPGLPLPLLLLLFLLSRLLR L in isoform H. CuratedAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061815, AF061813, AF061814 Genomic DNA Translation: AAC64270.1
BC123898 mRNA Translation: AAI23899.1
RefSeqiNP_001069688.1, NM_001076220.1
UniGeneiBt.1299

Genome annotation databases

GeneIDi540446
KEGGibta:540446

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061815, AF061813, AF061814 Genomic DNA Translation: AAC64270.1
BC123898 mRNA Translation: AAI23899.1
RefSeqiNP_001069688.1, NM_001076220.1
UniGeneiBt.1299

3D structure databases

ProteinModelPortaliP23795
SMRiP23795
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001517

Chemistry databases

BindingDBiP23795
ChEMBLiCHEMBL4768

Protein family/group databases

ESTHERibovin-ACHE AChE
MEROPSiS09.979

PTM databases

GlyConnecti8
UniCarbKBiP23795

Proteomic databases

PaxDbiP23795
PRIDEiP23795

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi540446
KEGGibta:540446

Organism-specific databases

CTDi43

Phylogenomic databases

eggNOGiKOG4389 Eukaryota
COG2272 LUCA
HOGENOMiHOG000091866
HOVERGENiHBG008839
InParanoidiP23795
KOiK01049

Enzyme and pathway databases

BRENDAi3.1.1.7 908

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR014788 AChE_tetra
IPR002018 CarbesteraseB
IPR019826 Carboxylesterase_B_AS
IPR019819 Carboxylesterase_B_CS
IPR000997 Cholinesterase
PfamiView protein in Pfam
PF08674 AChE_tetra, 1 hit
PF00135 COesterase, 1 hit
PRINTSiPR00878 CHOLNESTRASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD415333 AChE_tetra, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00122 CARBOXYLESTERASE_B_1, 1 hit
PS00941 CARBOXYLESTERASE_B_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACES_BOVIN
AccessioniPrimary (citable) accession number: P23795
Secondary accession number(s): O97579, Q08D79
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: July 11, 2001
Last modified: November 7, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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