UniProtKB - P23776 (EXG1_YEAST)
Protein
Glucan 1,3-beta-glucosidase I/II
Gene
EXG1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Glucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme hydrolyzes both 1,3-beta- and 1,6-beta-linkages and even has beta-glucosidase activity. It could also function biosynthetically as a transglycosylase.
Miscellaneous
Present with 4280 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose. EC:3.2.1.58
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 232 | Proton donorBy similarity | 1 | |
Active sitei | 334 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- beta-glucosidase activity Source: GO_Central
- glucan exo-1,3-beta-glucosidase activity Source: SGD
GO - Biological processi
- cellular glucan metabolic process Source: SGD
- fungal-type cell wall organization Source: SGD
- glucan catabolic process Source: GO_Central
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Cell wall biogenesis/degradation |
Protein family/group databases
CAZyi | GH5, Glycoside Hydrolase Family 5 |
CLAEi | EXG5A_YEAST |
Names & Taxonomyi
Protein namesi | Recommended name: Glucan 1,3-beta-glucosidase I/II (EC:3.2.1.58)Alternative name(s): Exo-1,3-beta-glucanase I/II Soluble cell wall protein 6 |
Gene namesi | Name:EXG1 Synonyms:BGL1, SCW6 Ordered Locus Names:YLR300W ORF Names:L8003.3 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YLR300W |
SGDi | S000004291, EXG1 |
Subcellular locationi
Cell Wall
- fungal-type cell wall Source: SGD
Extracellular region or secreted
- extracellular region Source: SGD
Vacuole
- fungal-type vacuole Source: SGD
Other locations
- cell surface Source: GO_Central
Keywords - Cellular componenti
Cell wall, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
PropeptideiPRO_0000007892 | 20 – 40 | 4 PublicationsAdd BLAST | 21 | |
ChainiPRO_0000007893 | 41 – 448 | Glucan 1,3-beta-glucosidase I/IIAdd BLAST | 408 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 165 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 325 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
Cleavage on pair of basic residues, GlycoproteinProteomic databases
MaxQBi | P23776 |
PaxDbi | P23776 |
PRIDEi | P23776 |
2D gel databases
COMPLUYEAST-2DPAGEi | P23776 |
PTM databases
iPTMneti | P23776 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 31564, 109 interactors |
DIPi | DIP-6384N |
IntActi | P23776, 3 interactors |
STRINGi | 4932.YLR300W |
Miscellaneous databases
RNActi | P23776, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23776 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23776 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 5 (cellulase A) family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QPYU, Eukaryota |
GeneTreei | ENSGT00940000176313 |
HOGENOMi | CLU_004624_0_1_1 |
InParanoidi | P23776 |
OMAi | HRRTWIT |
Family and domain databases
InterProi | View protein in InterPro IPR001547, Glyco_hydro_5 IPR018087, Glyco_hydro_5_CS IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00150, Cellulase, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00659, GLYCOSYL_HYDROL_F5, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P23776-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSLKTLLCT LLTVSSVLAT PVPARDPSSI QFVHEENKKR YYDYDHGSLG
60 70 80 90 100
EPIRGVNIGG WLLLEPYITP SLFEAFRTND DNDEGIPVDE YHFCQYLGKD
110 120 130 140 150
LAKSRLQSHW STFYQEQDFA NIASQGFNLV RIPIGYWAFQ TLDDDPYVSG
160 170 180 190 200
LQESYLDQAI GWARNNSLKV WVDLHGAAGS QNGFDNSGLR DSYKFLEDSN
210 220 230 240 250
LAVTTNVLNY ILKKYSAEEY LDTVIGIELI NEPLGPVLDM DKMKNDYLAP
260 270 280 290 300
AYEYLRNNIK SDQVIIIHDA FQPYNYWDDF MTENDGYWGV TIDHHHYQVF
310 320 330 340 350
ASDQLERSID EHIKVACEWG TGVLNESHWT VCGEFAAALT DCTKWLNSVG
360 370 380 390 400
FGARYDGSWV NGDQTSSYIG SCANNDDIAY WSDERKENTR RYVEAQLDAF
410 420 430 440
EMRGGWIIWC YKTESSLEWD AQRLMFNGLF PQPLTDRKYP NQCGTISN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 | Y → Q AA sequence (PubMed:9748433).Curated | 1 | |
Sequence conflicti | 46 | H → G AA sequence (PubMed:9748433).Curated | 1 | |
Sequence conflicti | 77 | R → H AA sequence (PubMed:2125957).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34341 Genomic DNA Translation: AAA34599.1 U17243 Genomic DNA Translation: AAB67345.1 AY693069 Genomic DNA Translation: AAT93088.1 BK006945 Genomic DNA Translation: DAA09610.1 |
PIRi | JN0118 |
RefSeqi | NP_013403.1, NM_001182188.1 |
Genome annotation databases
EnsemblFungii | YLR300W_mRNA; YLR300W; YLR300W |
GeneIDi | 851007 |
KEGGi | sce:YLR300W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34341 Genomic DNA Translation: AAA34599.1 U17243 Genomic DNA Translation: AAB67345.1 AY693069 Genomic DNA Translation: AAT93088.1 BK006945 Genomic DNA Translation: DAA09610.1 |
PIRi | JN0118 |
RefSeqi | NP_013403.1, NM_001182188.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H4P | X-ray | 1.75 | A/B | 41-448 | [»] | |
SMRi | P23776 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31564, 109 interactors |
DIPi | DIP-6384N |
IntActi | P23776, 3 interactors |
STRINGi | 4932.YLR300W |
Protein family/group databases
CAZyi | GH5, Glycoside Hydrolase Family 5 |
CLAEi | EXG5A_YEAST |
PTM databases
iPTMneti | P23776 |
2D gel databases
COMPLUYEAST-2DPAGEi | P23776 |
Proteomic databases
MaxQBi | P23776 |
PaxDbi | P23776 |
PRIDEi | P23776 |
Genome annotation databases
EnsemblFungii | YLR300W_mRNA; YLR300W; YLR300W |
GeneIDi | 851007 |
KEGGi | sce:YLR300W |
Organism-specific databases
EuPathDBi | FungiDB:YLR300W |
SGDi | S000004291, EXG1 |
Phylogenomic databases
eggNOGi | ENOG502QPYU, Eukaryota |
GeneTreei | ENSGT00940000176313 |
HOGENOMi | CLU_004624_0_1_1 |
InParanoidi | P23776 |
OMAi | HRRTWIT |
Miscellaneous databases
EvolutionaryTracei | P23776 |
PROi | PR:P23776 |
RNActi | P23776, protein |
Family and domain databases
InterProi | View protein in InterPro IPR001547, Glyco_hydro_5 IPR018087, Glyco_hydro_5_CS IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00150, Cellulase, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00659, GLYCOSYL_HYDROL_F5, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EXG1_YEAST | |
Accessioni | P23776Primary (citable) accession number: P23776 Secondary accession number(s): D6VYU4, Q9UR92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries