Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Trans-acting T-cell-specific transcription factor GATA-3

Gene

Gata3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri263 – 287GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri317 – 341GATA-type 2PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processImmunity, Innate immunity, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-9018519 Estrogen-dependent gene expression
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-acting T-cell-specific transcription factor GATA-3
Alternative name(s):
GATA-binding factor 3
Gene namesi
Name:Gata3
Synonyms:Gata-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95663 Gata3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi344Y → A: Dramatically decreased Th2 cell differentiation. 1 Publication1
Mutagenesisi346K → A: Moderately decreased Th2 cell differentiation. 1 Publication1
Mutagenesisi348H → A: Dramatically decreased Th2 cell differentiation. 1 Publication1
Mutagenesisi352R → A: Fails to induce Th2 cytokine production. 1 Publication1
Mutagenesisi353P → A or K: Fails to induce Th2 cytokine production. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834091 – 443Trans-acting T-cell-specific transcription factor GATA-3Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei114PhosphoserineBy similarity1
Modified residuei161PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP23772
PRIDEiP23772

PTM databases

iPTMnetiP23772
PhosphoSitePlusiP23772

Expressioni

Tissue specificityi

T-cell specific.

Gene expression databases

BgeeiENSMUSG00000015619 Expressed in 265 organ(s), highest expression level in embryo
CleanExiMM_GATA3
ExpressionAtlasiP23772 baseline and differential
GenevisibleiP23772 MM

Interactioni

Subunit structurei

Interacts with TBX21 ('Tyr-525' phosphorylated form).3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199840, 4 interactors
DIPiDIP-29712N
IntActiP23772, 4 interactors
STRINGi10090.ENSMUSP00000100041

Structurei

Secondary structure

1443
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP23772
SMRiP23772
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23772

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 257Interaction with TBX211 PublicationAdd BLAST257
Regioni288 – 316Flexible linkerBy similarityAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi344 – 353YxKxHxxxRP10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi136 – 139Poly-Ser4
Compositional biasi322 – 326Poly-Thr5

Domaini

Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation (By similarity).By similarity
The YxKxHxxxRP motif is critical for DNA-binding and function.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri263 – 287GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri317 – 341GATA-type 2PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601 Eukaryota
COG5641 LUCA
GeneTreeiENSGT00760000119221
HOGENOMiHOG000047701
HOVERGENiHBG051705
InParanoidiP23772
KOiK17895
OMAiRWMSHHP
OrthoDBiEOG091G0AUR
PhylomeDBiP23772
TreeFamiTF315391

Family and domain databases

CDDicd00202 ZnF_GATA, 2 hits
Gene3Di3.30.50.10, 2 hits
InterProiView protein in InterPro
IPR029521 GATA-3
IPR016374 TF_GATA-2/3
IPR039355 Transcription_factor_GATA
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PANTHERiPTHR10071 PTHR10071, 1 hit
PTHR10071:SF106 PTHR10071:SF106, 1 hit
PfamiView protein in Pfam
PF00320 GATA, 2 hits
PIRSFiPIRSF003027 TF_GATA-1/2/3, 1 hit
PRINTSiPR00619 GATAZNFINGER
SMARTiView protein in SMART
SM00401 ZnF_GATA, 2 hits
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 2 hits
PS50114 GATA_ZN_FINGER_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P23772-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LGHSYMEAQY PLTEEVDVLF
60 70 80 90 100
NIDGQGNHVP SYYGNSVRAT VQRYPPTHHG SQVCRPPLLH GSLPWLDGGK
110 120 130 140 150
ALSSHHTASP WNLSPFSKTS IHHGSPGPLS VYPPASSSSL AAGHSSPHLF
160 170 180 190 200
TFPPTPPKDV SPDPSLSTPG SAGSARQDEK ECLKYQVQLP DSMKLETSHS
210 220 230 240 250
RGSMTTLGGA SSSAHHPITT YPPYVPEYSS GLFPPSSLLG GSPTGFGCKS
260 270 280 290 300
RPKARSSTEG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL
310 320 330 340 350
IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI
360 370 380 390 400
NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD ALEDFPKSSS FNPAALSRHM
410 420 430 440
SSLSHISPFS HSSHMLTTPT PMHPPSGLSF GPHHPSSMVT AMG
Length:443
Mass (Da):47,968
Last modified:November 1, 1991 - v1
Checksum:i980E58FB9872E560
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6QTY0F6QTY0_MOUSE
Trans-acting T-cell-specific transc...
Gata3
119Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55123 mRNA Translation: CAA38917.1
BC062915 mRNA Translation: AAH62915.1
CCDSiCCDS15674.1
PIRiB39794
RefSeqiNP_032117.1, NM_008091.3
XP_006497417.1, XM_006497354.1
UniGeneiMm.313866

Genome annotation databases

EnsembliENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619
GeneIDi14462
KEGGimmu:14462
UCSCiuc008ihf.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55123 mRNA Translation: CAA38917.1
BC062915 mRNA Translation: AAH62915.1
CCDSiCCDS15674.1
PIRiB39794
RefSeqiNP_032117.1, NM_008091.3
XP_006497417.1, XM_006497354.1
UniGeneiMm.313866

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DFVX-ray3.10C/D308-370[»]
3DFXX-ray2.70A/B308-370[»]
ProteinModelPortaliP23772
SMRiP23772
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199840, 4 interactors
DIPiDIP-29712N
IntActiP23772, 4 interactors
STRINGi10090.ENSMUSP00000100041

PTM databases

iPTMnetiP23772
PhosphoSitePlusiP23772

Proteomic databases

PaxDbiP23772
PRIDEiP23772

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619
GeneIDi14462
KEGGimmu:14462
UCSCiuc008ihf.1 mouse

Organism-specific databases

CTDi2625
MGIiMGI:95663 Gata3

Phylogenomic databases

eggNOGiKOG1601 Eukaryota
COG5641 LUCA
GeneTreeiENSGT00760000119221
HOGENOMiHOG000047701
HOVERGENiHBG051705
InParanoidiP23772
KOiK17895
OMAiRWMSHHP
OrthoDBiEOG091G0AUR
PhylomeDBiP23772
TreeFamiTF315391

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-9018519 Estrogen-dependent gene expression
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

EvolutionaryTraceiP23772
PROiPR:P23772
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015619 Expressed in 265 organ(s), highest expression level in embryo
CleanExiMM_GATA3
ExpressionAtlasiP23772 baseline and differential
GenevisibleiP23772 MM

Family and domain databases

CDDicd00202 ZnF_GATA, 2 hits
Gene3Di3.30.50.10, 2 hits
InterProiView protein in InterPro
IPR029521 GATA-3
IPR016374 TF_GATA-2/3
IPR039355 Transcription_factor_GATA
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PANTHERiPTHR10071 PTHR10071, 1 hit
PTHR10071:SF106 PTHR10071:SF106, 1 hit
PfamiView protein in Pfam
PF00320 GATA, 2 hits
PIRSFiPIRSF003027 TF_GATA-1/2/3, 1 hit
PRINTSiPR00619 GATAZNFINGER
SMARTiView protein in SMART
SM00401 ZnF_GATA, 2 hits
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 2 hits
PS50114 GATA_ZN_FINGER_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGATA3_MOUSE
AccessioniPrimary (citable) accession number: P23772
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 7, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again