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Protein

Trans-acting T-cell-specific transcription factor GATA-3

Gene

GATA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri263 – 287GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri317 – 341GATA-type 2PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processImmunity, Innate immunity, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-5689880 Ub-specific processing proteases
R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-9018519 Estrogen-dependent gene expression
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SignaLinkiP23771
SIGNORiP23771

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-acting T-cell-specific transcription factor GATA-3
Alternative name(s):
GATA-binding factor 3
Gene namesi
Name:GATA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000107485.15
HGNCiHGNC:4172 GATA3
MIMi131320 gene
neXtProtiNX_P23771

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Hypoparathyroidism, sensorineural deafness, and renal disease (HDR)3 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disease characterized by steroid-resistant nephrosis with progressive renal failure, hypoparathyroidism, sensorineural deafness, and renal dysplasia.
See also OMIM:146255
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017818274W → R in HDR. 1 PublicationCorresponds to variant dbSNP:rs104894163Ensembl.1
Natural variantiVAR_075427298R → Q in HDR; loss of enhancer activity on PTH gene promoter and on GATA responsive element. 1 Publication1

Keywords - Diseasei

Deafness, Disease mutation

Organism-specific databases

DisGeNETi2625
MalaCardsiGATA3
MIMi146255 phenotype
OpenTargetsiENSG00000107485
Orphaneti2237 Hypoparathyroidism-sensorineural deafness-renal disease syndrome
99860 Precursor B-cell acute lymphoblastic leukemia
PharmGKBiPA28586

Polymorphism and mutation databases

BioMutaiGATA3
DMDMi120962

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834081 – 443Trans-acting T-cell-specific transcription factor GATA-3Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei115PhosphoserineCombined sources1
Modified residuei162PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP23771
MaxQBiP23771
PaxDbiP23771
PeptideAtlasiP23771
PRIDEiP23771
ProteomicsDBi54162
54163 [P23771-2]

PTM databases

iPTMnetiP23771
PhosphoSitePlusiP23771

Expressioni

Tissue specificityi

T-cells and endothelial cells.

Gene expression databases

BgeeiENSG00000107485 Expressed in 166 organ(s), highest expression level in endometrium epithelium
CleanExiHS_GATA3
GenevisibleiP23771 HS

Organism-specific databases

HPAiCAB016217
HPA029730

Interactioni

Subunit structurei

Interacts with TBX21 ('Tyr-530' phosphorylated form).1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108895, 23 interactors
DIPiDIP-61302N
IntActiP23771, 40 interactors
MINTiP23771
STRINGi9606.ENSP00000368632

Structurei

Secondary structure

1443
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP23771
SMRiP23771
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 258Interaction with TBX21By similarityAdd BLAST258
Regioni288 – 316Flexible linkerAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi344 – 353YxKxHxxxRPBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi137 – 142Poly-Ser6
Compositional biasi322 – 326Poly-Thr5

Domaini

Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation.
The YxKxHxxxRP motif is critical for DNA-binding and function.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri263 – 287GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri317 – 341GATA-type 2PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601 Eukaryota
COG5641 LUCA
GeneTreeiENSGT00760000119221
HOGENOMiHOG000047701
HOVERGENiHBG051705
InParanoidiP23771
KOiK17895
OMAiRWMSHHP
OrthoDBiEOG091G0AUR
PhylomeDBiP23771
TreeFamiTF315391

Family and domain databases

CDDicd00202 ZnF_GATA, 2 hits
Gene3Di3.30.50.10, 2 hits
InterProiView protein in InterPro
IPR029521 GATA-3
IPR016374 TF_GATA-2/3
IPR039355 Transcription_factor_GATA
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PANTHERiPTHR10071 PTHR10071, 1 hit
PTHR10071:SF106 PTHR10071:SF106, 1 hit
PfamiView protein in Pfam
PF00320 GATA, 2 hits
PIRSFiPIRSF003027 TF_GATA-1/2/3, 1 hit
PRINTSiPR00619 GATAZNFINGER
SMARTiView protein in SMART
SM00401 ZnF_GATA, 2 hits
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 2 hits
PS50114 GATA_ZN_FINGER_2, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P23771-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LSHSYMDAAQ YPLPEEVDVL
60 70 80 90 100
FNIDGQGNHV PPYYGNSVRA TVQRYPPTHH GSQVCRPPLL HGSLPWLDGG
110 120 130 140 150
KALGSHHTAS PWNLSPFSKT SIHHGSPGPL SVYPPASSSS LSGGHASPHL
160 170 180 190 200
FTFPPTPPKD VSPDPSLSTP GSAGSARQDE KECLKYQVPL PDSMKLESSH
210 220 230 240 250
SRGSMTALGG ASSSTHHPIT TYPPYVPEYS SGLFPPSSLL GGSPTGFGCK
260 270 280 290 300
SRPKARSSTG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL
310 320 330 340 350
IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI
360 370 380 390 400
NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD SLEDFPKNSS FNPAALSRHM
410 420 430 440
SSLSHISPFS HSSHMLTTPT PMHPPSSLSF GPHHPSSMVT AMG
Length:443
Mass (Da):47,916
Last modified:November 1, 1991 - v1
Checksum:iF24C58681E1D02A5
GO
Isoform 2 (identifier: P23771-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-259: T → TE

Show »
Length:444
Mass (Da):48,045
Checksum:i506051438132F1D0
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2R8Y2A9A0A2R8Y2A9_HUMAN
Trans-acting T-cell-specific transc...
GATA3
143Annotation score:
A0A2R8Y4T2A0A2R8Y4T2_HUMAN
Trans-acting T-cell-specific transc...
GATA3
80Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11V → L in AAA35870 (PubMed:1871134).Curated1
Sequence conflicti102A → V in CAA38877 (PubMed:1827068).Curated1
Sequence conflicti173A → G in CAA38877 (PubMed:1827068).Curated1
Sequence conflicti219I → Y in AAA35870 (PubMed:1871134).Curated1
Sequence conflicti225 – 226YV → TC in AAA35870 (PubMed:1871134).Curated2
Sequence conflicti285 – 286NA → RR in AAA35870 (PubMed:1871134).Curated2
Sequence conflicti425P → A in AAA35870 (PubMed:1871134).Curated1
Sequence conflicti441A → G in AAA35870 (PubMed:1871134).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_019202242G → S1 PublicationCorresponds to variant dbSNP:rs11567901Ensembl.1
Natural variantiVAR_017818274W → R in HDR. 1 PublicationCorresponds to variant dbSNP:rs104894163Ensembl.1
Natural variantiVAR_075427298R → Q in HDR; loss of enhancer activity on PTH gene promoter and on GATA responsive element. 1 Publication1
Natural variantiVAR_033025366R → L in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001598259T → TE in isoform 2. 3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58072 mRNA Translation: CAA41102.1
X55037 mRNA Translation: CAA38877.1
X55122 mRNA Translation: CAA38916.1
M69106 mRNA Translation: AAA35870.1
AY497006 Genomic DNA Translation: AAR32096.1
AL390294 Genomic DNA No translation available.
CH471072 Genomic DNA Translation: EAW86367.1
CH471072 Genomic DNA Translation: EAW86368.1
BC003070 mRNA Translation: AAH03070.1
BC006793 mRNA Translation: AAH06793.1
CCDSiCCDS31143.1 [P23771-2]
CCDS7083.1 [P23771-1]
PIRiA39794
RefSeqiNP_001002295.1, NM_001002295.1 [P23771-2]
NP_002042.1, NM_002051.2 [P23771-1]
XP_005252499.1, XM_005252442.2 [P23771-2]
XP_005252500.1, XM_005252443.4 [P23771-2]
UniGeneiHs.524134

Genome annotation databases

EnsembliENST00000346208; ENSP00000341619; ENSG00000107485 [P23771-1]
ENST00000379328; ENSP00000368632; ENSG00000107485 [P23771-2]
ENST00000645492; ENSP00000494926; ENSG00000107485 [P23771-2]
GeneIDi2625
KEGGihsa:2625
UCSCiuc001ijz.4 human [P23771-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

SeattleSNPs
Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58072 mRNA Translation: CAA41102.1
X55037 mRNA Translation: CAA38877.1
X55122 mRNA Translation: CAA38916.1
M69106 mRNA Translation: AAA35870.1
AY497006 Genomic DNA Translation: AAR32096.1
AL390294 Genomic DNA No translation available.
CH471072 Genomic DNA Translation: EAW86367.1
CH471072 Genomic DNA Translation: EAW86368.1
BC003070 mRNA Translation: AAH03070.1
BC006793 mRNA Translation: AAH06793.1
CCDSiCCDS31143.1 [P23771-2]
CCDS7083.1 [P23771-1]
PIRiA39794
RefSeqiNP_001002295.1, NM_001002295.1 [P23771-2]
NP_002042.1, NM_002051.2 [P23771-1]
XP_005252499.1, XM_005252442.2 [P23771-2]
XP_005252500.1, XM_005252443.4 [P23771-2]
UniGeneiHs.524134

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HC7X-ray2.65A/B260-370[»]
4HC9X-ray1.60A260-370[»]
4HCAX-ray2.80A260-370[»]
ProteinModelPortaliP23771
SMRiP23771
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108895, 23 interactors
DIPiDIP-61302N
IntActiP23771, 40 interactors
MINTiP23771
STRINGi9606.ENSP00000368632

PTM databases

iPTMnetiP23771
PhosphoSitePlusiP23771

Polymorphism and mutation databases

BioMutaiGATA3
DMDMi120962

Proteomic databases

EPDiP23771
MaxQBiP23771
PaxDbiP23771
PeptideAtlasiP23771
PRIDEiP23771
ProteomicsDBi54162
54163 [P23771-2]

Protocols and materials databases

DNASUi2625
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000346208; ENSP00000341619; ENSG00000107485 [P23771-1]
ENST00000379328; ENSP00000368632; ENSG00000107485 [P23771-2]
ENST00000645492; ENSP00000494926; ENSG00000107485 [P23771-2]
GeneIDi2625
KEGGihsa:2625
UCSCiuc001ijz.4 human [P23771-1]

Organism-specific databases

CTDi2625
DisGeNETi2625
EuPathDBiHostDB:ENSG00000107485.15
GeneCardsiGATA3
HGNCiHGNC:4172 GATA3
HPAiCAB016217
HPA029730
MalaCardsiGATA3
MIMi131320 gene
146255 phenotype
neXtProtiNX_P23771
OpenTargetsiENSG00000107485
Orphaneti2237 Hypoparathyroidism-sensorineural deafness-renal disease syndrome
99860 Precursor B-cell acute lymphoblastic leukemia
PharmGKBiPA28586
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1601 Eukaryota
COG5641 LUCA
GeneTreeiENSGT00760000119221
HOGENOMiHOG000047701
HOVERGENiHBG051705
InParanoidiP23771
KOiK17895
OMAiRWMSHHP
OrthoDBiEOG091G0AUR
PhylomeDBiP23771
TreeFamiTF315391

Enzyme and pathway databases

ReactomeiR-HSA-5689880 Ub-specific processing proteases
R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-9018519 Estrogen-dependent gene expression
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SignaLinkiP23771
SIGNORiP23771

Miscellaneous databases

GeneWikiiGATA3
GenomeRNAii2625
PROiPR:P23771
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107485 Expressed in 166 organ(s), highest expression level in endometrium epithelium
CleanExiHS_GATA3
GenevisibleiP23771 HS

Family and domain databases

CDDicd00202 ZnF_GATA, 2 hits
Gene3Di3.30.50.10, 2 hits
InterProiView protein in InterPro
IPR029521 GATA-3
IPR016374 TF_GATA-2/3
IPR039355 Transcription_factor_GATA
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PANTHERiPTHR10071 PTHR10071, 1 hit
PTHR10071:SF106 PTHR10071:SF106, 1 hit
PfamiView protein in Pfam
PF00320 GATA, 2 hits
PIRSFiPIRSF003027 TF_GATA-1/2/3, 1 hit
PRINTSiPR00619 GATAZNFINGER
SMARTiView protein in SMART
SM00401 ZnF_GATA, 2 hits
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 2 hits
PS50114 GATA_ZN_FINGER_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGATA3_HUMAN
AccessioniPrimary (citable) accession number: P23771
Secondary accession number(s): Q5VWG7, Q5VWG8, Q96J16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 7, 2018
This is version 194 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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