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Protein

Vesicle-associated membrane protein 1

Gene

VAMP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei60 – 61(Microbial infection) Cleavage; by C.botulinum neurotoxin type F (BoNT/F, botF)1 Publication2
Sitei61 – 62(Microbial infection) Cleavage; by C.botulinum neurotoxin type D (BoNT/D, botD)1 Publication2
Sitei68 – 69(Microbial infection) Cleavage; by C.botulinum neurotoxin type X (BoNT/X)1 Publication2
Sitei78 – 79(Microbial infection) Cleavage; by C.botulinum neurotoxin type B (BoNT/B, botB)1 Publication2

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-5250955 Toxicity of botulinum toxin type D (BoNT/D)
R-HSA-5250981 Toxicity of botulinum toxin type F (BoNT/F)
R-HSA-5250989 Toxicity of botulinum toxin type G (BoNT/G)

Protein family/group databases

TCDBi1.F.1.1.1 the synaptosomal vesicle fusion pore (svf-pore) family

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 1
Short name:
VAMP-1
Alternative name(s):
Synaptobrevin-1
Gene namesi
Name:VAMP1
Synonyms:SYB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000139190.16
HGNCiHGNC:12642 VAMP1
MIMi185880 gene
neXtProtiNX_P23763

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 96CytoplasmicSequence analysisAdd BLAST96
Transmembranei97 – 116Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST20
Topological domaini117 – 118VesicularSequence analysis2

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Mitochondrion, Mitochondrion outer membrane, Synapse, Synaptosome

Pathology & Biotechi

Involvement in diseasei

Spastic ataxia 1, autosomal dominant (SPAX1)1 Publication
The disease is caused by mutations affecting the gene represented in this entry. A mutation affecting a critical donor site for the splicing of VAMP1 isoforms leads to the loss of neuron-specific isoform 1 and subsequently results in haploinsufficiency (PubMed:22958904). Therefore, there would be less neurotransmitter exocytosis in specific regions of the brain, causing the symptoms of SPAX1.1 Publication
Disease descriptionAn autosomal dominant form of spastic ataxia, a progressive neurodegenerative disorder characterized by lower-limb spasticity and generalized ataxia with dysarthria, impaired ocular movements, and gait disturbance.
See also OMIM:108600

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42E → D: No change in susceptibility to C.botulinum BoNT/B, BoNT/D or BoNT/F. 1 Publication1
Mutagenesisi48I → M: 1000-fold increase in susceptibility to C.botulinum BoNT/D, no change in susceptibility to BoNT/B or BoNT/F. 1 Publication1
Mutagenesisi81S → T: No change in susceptibility to C.botulinum BoNT/B, BoNT/D or BoNT/F. 1 Publication1

Keywords - Diseasei

Neurodegeneration

Organism-specific databases

DisGeNETi6843
MalaCardsiVAMP1
MIMi108600 phenotype
OpenTargetsiENSG00000139190
Orphaneti251282 Autosomal dominant spastic ataxia type 1
PharmGKBiPA37266

Chemistry databases

DrugBankiDB00042 Botulinum Toxin Type B

Polymorphism and mutation databases

BioMutaiVAMP1
DMDMi135093

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067191 – 118Vesicle-associated membrane protein 1Add BLAST118

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63PhosphoserineBy similarity1

Post-translational modificationi

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which probably hydrolyzes the 78-Gln-|-Phe-79 bond and inhibits neurotransmitter release (PubMed:22289120).1 Publication1 Publication
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 61-Arg-|-Leu-62 bond and inhibits neurotransmitter release (PubMed:22289120). BoNT/D has low catalytic activity on this protein due to its sequence (PubMed:22289120). Note that humans are not known to be infected by C.botulinum type D.Curated1 Publication
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which probably hydrolyzes the 60-Gln-|-Lys-61 bond and inhibits neurotransmitter release (PubMed:22289120).1 Publication1 Publication
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which probably hydrolyzes the 68-Arg-|-Ala-69 bond and inhibits neurotransmitter release (PubMed:29540745). It remains unknown whether BoNT/X is ever produced, or what organisms it targets.1 Publication1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP23763
MaxQBiP23763
PaxDbiP23763
PeptideAtlasiP23763
PRIDEiP23763
ProteomicsDBi54157
54158 [P23763-2]
54159 [P23763-3]

PTM databases

iPTMnetiP23763
PhosphoSitePlusiP23763
SwissPalmiP23763

Miscellaneous databases

PMAP-CutDBiP23763

Expressioni

Tissue specificityi

Nervous system, skeletal muscle and adipose tissue.

Gene expression databases

BgeeiENSG00000139190
CleanExiHS_VAMP1
ExpressionAtlasiP23763 baseline and differential
GenevisibleiP23763 HS

Organism-specific databases

HPAiCAB002779
CAB016119
CAB079000

Interactioni

Subunit structurei

Interacts with VAPA and VAPB.

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112710, 10 interactors
CORUMiP23763
IntActiP23763, 22 interactors
STRINGi9606.ENSP00000379602

Structurei

3D structure databases

ProteinModelPortaliP23763
SMRiP23763
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 93v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOVERGENiHBG006675
InParanoidiP23763
KOiK08510
OMAiMIMMGVI
OrthoDBiEOG091G0MPE
PhylomeDBiP23763
TreeFamiTF313666

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23763-1) [UniParc]FASTAAdd to basket
Also known as: VAMP-1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAPAQPPAE GTEGTAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIIRV
60 70 80 90 100
NVDKVLERDQ KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM
110
LGAICAIIVV VIVIYFFT
Length:118
Mass (Da):12,902
Last modified:November 1, 1991 - v1
Checksum:i64CE9615447B686B
GO
Isoform 2 (identifier: P23763-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYFFT → SKYR

Show »
Length:117
Mass (Da):12,765
Checksum:iF3449D319B686B1A
GO
Isoform 3 (identifier: P23763-2) [UniParc]FASTAAdd to basket
Also known as: VAMP-1B

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYFFT → RRD

Show »
Length:116
Mass (Da):12,658
Checksum:i957A002B686B1AE6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103A → T in CAA88760 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029185114 – 118IYFFT → SKYR in isoform 2. 2 Publications5
Alternative sequenceiVSP_006325114 – 118IYFFT → RRD in isoform 3. 2 Publications5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36200
, M36196, M36197, M36198, M36199 Genomic DNA Translation: AAA60603.1
AF060538 mRNA Translation: AAC28336.1
Z48924 mRNA Translation: CAA88760.1
CR542214 mRNA Translation: CAG47010.1
CR542231 mRNA Translation: CAG47027.1
CR749485 mRNA Translation: CAH18312.1
AC005840 Genomic DNA No translation available.
CH471116 Genomic DNA Translation: EAW88792.1
CH471116 Genomic DNA Translation: EAW88795.1
CH471116 Genomic DNA Translation: EAW88796.1
BC023286 mRNA Translation: AAH23286.1
CCDSiCCDS31731.1 [P23763-3]
CCDS41740.1 [P23763-1]
CCDS44809.1 [P23763-2]
PIRiA38315
S52747
RefSeqiNP_001284367.1, NM_001297438.1
NP_055046.1, NM_014231.4 [P23763-1]
NP_058439.1, NM_016830.3 [P23763-2]
NP_954740.1, NM_199245.2 [P23763-3]
UniGeneiHs.20021

Genome annotation databases

EnsembliENST00000361716; ENSP00000355122; ENSG00000139190 [P23763-3]
ENST00000396308; ENSP00000379602; ENSG00000139190 [P23763-1]
ENST00000400911; ENSP00000383702; ENSG00000139190 [P23763-2]
GeneIDi6843
KEGGihsa:6843
UCSCiuc001qoj.4 human [P23763-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVAMP1_HUMAN
AccessioniPrimary (citable) accession number: P23763
Secondary accession number(s): A8MVP3
, D3DUR3, O75468, Q15857, Q6FG94, Q8IVC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 18, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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