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Protein

M-phase inducer phosphatase

Gene

MIH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Terminates the cell cycle delay. Reverses the CDC28 phosphorylation catalyzed by SWE1.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei320By similarity1

GO - Molecular functioni

  • protein tyrosine phosphatase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32741-MONOMER
ReactomeiR-SCE-156711 Polo-like kinase mediated events
R-SCE-176187 Activation of ATR in response to replication stress
R-SCE-5625740 RHO GTPases activate PKNs
R-SCE-5689880 Ub-specific processing proteases
R-SCE-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain
R-SCE-69202 Cyclin E associated events during G1/S transition
R-SCE-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-69656 Cyclin A:Cdk2-associated events at S phase entry
R-SCE-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase (EC:3.1.3.48)
Alternative name(s):
Mitosis initiation protein MIH1
Mitotic inducer homolog
Gene namesi
Name:MIH1
Ordered Locus Names:YMR036C
ORF Names:YM9532.01C, YM9973.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR036C
SGDiS000004639 MIH1

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001986621 – 554M-phase inducer phosphataseAdd BLAST554

Proteomic databases

PaxDbiP23748
PRIDEiP23748

PTM databases

iPTMnetiP23748

Interactioni

Protein-protein interaction databases

BioGridi35208, 157 interactors
DIPiDIP-2713N
IntActiP23748, 21 interactors
MINTiP23748
STRINGi4932.YMR036C

Structurei

3D structure databases

ProteinModelPortaliP23748
SMRiP23748
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini261 – 373RhodanesePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000018747
InParanoidiP23748
KOiK02555
OrthoDBiEOG092C2GWG

Family and domain databases

CDDicd01530 Cdc25, 1 hit
Gene3Di3.40.250.10, 1 hit
InterProiView protein in InterPro
IPR000751 MPI_Phosphatase
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
PfamiView protein in Pfam
PF00581 Rhodanese, 1 hit
PRINTSiPR00716 MPIPHPHTASE
SMARTiView protein in SMART
SM00450 RHOD, 1 hit
SUPFAMiSSF52821 SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit

Sequencei

Sequence statusi: Complete.

P23748-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNNIFHGTED ECANEDVLSF QKISLKSPFG KKKNIFRNVQ TFFKSKSKHS
60 70 80 90 100
NVDDDLINKE NLAFDKSPLL TNHRSKEIDG PSPNIKQLGH RDELDENENE
110 120 130 140 150
NDDIVLSMHF ASQTLQSPTR NSSRRSLTNN RDNDLLSRIK YPGSPQRSSS
160 170 180 190 200
FSRSRSLSRK PSMNSSSNSS RRVQRQDGKI PRSSRKSSQK FSNITQNTLN
210 220 230 240 250
FTSASSSPLA PNSVGVKCFE SCLAKTQIPY YYDDRNSNDF FPRISPETLK
260 270 280 290 300
NILQNNMCES FYNSCRIIDC RFEYEYTGGH IINSVNIHSR DELEYEFIHK
310 320 330 340 350
VLHSDTSNNN TLPTLLIIHC EFSSHRGPSL ASHLRNCDRI INQDHYPKLF
360 370 380 390 400
YPDILILDGG YKAVFDNFPE LCYPRQYVGM NSQENLLNCE QEMDKFRRES
410 420 430 440 450
KRFATKNNSF RKLASPSNPN FFYRDSHQSS TTMASSALSF RFEPPPKLSL
460 470 480 490 500
NHRRVSSGSS LNSSESTGDE NFFPILSKSS MSSNSNLSTS HMLLMDGLDT
510 520 530 540 550
PSYFSFEDER GNHQQVSGDE EQDGDFTFVG SDREDLPRPA RRSLFPSLET

EDKK
Length:554
Mass (Da):63,358
Last modified:November 1, 1995 - v2
Checksum:iDBD85D02CF48CA6F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti238 – 239ND → MT in AAA57223 (PubMed:2649252).Curated2
Sequence conflicti241 – 248FPRISPET → SLEFLQKR in AAA57223 (PubMed:2649252).Curated8
Sequence conflicti365 – 367FDN → LT in AAA57223 (PubMed:2649252).Curated3
Sequence conflicti475 – 554ILSKS…TEDKK → S in AAA57223 (PubMed:2649252).CuratedAdd BLAST80

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04846 Genomic DNA Translation: AAA57223.1
Z48502 Genomic DNA Translation: CAA88402.1
Z49213 Genomic DNA Translation: CAA89152.1
BK006946 Genomic DNA Translation: DAA09935.1
PIRiA32386
RefSeqiNP_013750.1, NM_001182533.1

Genome annotation databases

EnsemblFungiiYMR036C; YMR036C; YMR036C
GeneIDi855052
KEGGisce:YMR036C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04846 Genomic DNA Translation: AAA57223.1
Z48502 Genomic DNA Translation: CAA88402.1
Z49213 Genomic DNA Translation: CAA89152.1
BK006946 Genomic DNA Translation: DAA09935.1
PIRiA32386
RefSeqiNP_013750.1, NM_001182533.1

3D structure databases

ProteinModelPortaliP23748
SMRiP23748
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35208, 157 interactors
DIPiDIP-2713N
IntActiP23748, 21 interactors
MINTiP23748
STRINGi4932.YMR036C

PTM databases

iPTMnetiP23748

Proteomic databases

PaxDbiP23748
PRIDEiP23748

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR036C; YMR036C; YMR036C
GeneIDi855052
KEGGisce:YMR036C

Organism-specific databases

EuPathDBiFungiDB:YMR036C
SGDiS000004639 MIH1

Phylogenomic databases

GeneTreeiENSGT00390000018747
InParanoidiP23748
KOiK02555
OrthoDBiEOG092C2GWG

Enzyme and pathway databases

BioCyciYEAST:G3O-32741-MONOMER
ReactomeiR-SCE-156711 Polo-like kinase mediated events
R-SCE-176187 Activation of ATR in response to replication stress
R-SCE-5625740 RHO GTPases activate PKNs
R-SCE-5689880 Ub-specific processing proteases
R-SCE-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain
R-SCE-69202 Cyclin E associated events during G1/S transition
R-SCE-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-69656 Cyclin A:Cdk2-associated events at S phase entry
R-SCE-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Miscellaneous databases

PROiPR:P23748

Family and domain databases

CDDicd01530 Cdc25, 1 hit
Gene3Di3.40.250.10, 1 hit
InterProiView protein in InterPro
IPR000751 MPI_Phosphatase
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
PfamiView protein in Pfam
PF00581 Rhodanese, 1 hit
PRINTSiPR00716 MPIPHPHTASE
SMARTiView protein in SMART
SM00450 RHOD, 1 hit
SUPFAMiSSF52821 SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMPIP_YEAST
AccessioniPrimary (citable) accession number: P23748
Secondary accession number(s): D6VZL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1995
Last modified: September 12, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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