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UniProtKB - P23743 (DGKA_HUMAN)

Entry version 211 (23 Feb 2022)
Sequence version 3 (15 Dec 2009)
Previous versions | rss
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Protein

Diacylglycerol kinase alpha

Gene

DGKA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:2175712, PubMed:15544348).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:2175712, PubMed:15544348).

Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group (PubMed:15544348).

Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol (PubMed:22627129).

Curated3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Stimulated by calcium and phosphatidylserine.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycerolipid metabolism

This protein is involved in the pathway glycerolipid metabolism, which is part of Lipid metabolism.3 Publications
View all proteins of this organism that are known to be involved in the pathway glycerolipid metabolism and in Lipid metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) of the calcium-binding region(s) within the protein. One common calcium-binding motif is the EF-hand, but other calcium-binding motifs also exist.<p><a href='/help/ca_bind' target='_top'>More...</a></p>Calcium bindingi123 – 1341PROSITE-ProRule annotation1 PublicationAdd BLAST12
Calcium bindingi168 – 1792PROSITE-ProRule annotation1 PublicationAdd BLAST12
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri205 – 253Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri269 – 319Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Transferase
Biological processLipid metabolism
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.7.1.107, 2681

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		P23743

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-114508, Effects of PIP2 hydrolysis

SABIO-RK: Biochemical Reaction Kinetics Database

More...SABIO-RKi		P23743

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		P23743

SIGNOR Signaling Network Open Resource

More...SIGNORi		P23743

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00230

Chemistry databases

SwissLipids knowledge resource for lipid biology

More...SwissLipidsi		SLP:000000555
SLP:000000739

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Diacylglycerol kinase alpha (EC:2.7.1.1072 Publications)
Short name:
DAG kinase alpha
Alternative name(s):
80 kDa diacylglycerol kinase
Diglyceride kinase alpha
Short name:
DGK-alpha
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:DGKA
Synonyms:DAGK, DAGK1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 12

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:2849, DGKA

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		125855, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P23743

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000065357

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi134E → Q: Binds calcium but with decreased affinity. 1 Publication1
Mutagenesisi179E → Q: Loss of calcium-binding. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		1606

Open Targets

More...OpenTargetsi		ENSG00000065357

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA27310

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P23743, Tbio

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL4105787

Drug and drug target database

More...DrugBanki		DB14001, alpha-Tocopherol succinate
DB00163, Vitamin E

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		DGKA

Domain mapping of disease mutations (DMDM)

More...DMDMi		281185505

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002184531 – 735Diacylglycerol kinase alphaAdd BLAST735

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei484N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		P23743

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P23743

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P23743

MaxQB - The MaxQuant DataBase

More...MaxQBi		P23743

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P23743

PeptideAtlas

More...PeptideAtlasi		P23743

PRoteomics IDEntifications database

More...PRIDEi		P23743

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		54146 [P23743-1]
54147 [P23743-2]

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		P23743, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P23743

MetOSite database of methionine sulfoxide sites

More...MetOSitei		P23743

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P23743

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in lymphocytes.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000065357, Expressed in small intestine Peyer's patch and 221 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P23743, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P23743, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000065357, Tissue enhanced (esophagus, lymphoid tissue)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

2 Publications

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		107976, 8 interactors

Protein interaction database and analysis system

More...IntActi		P23743, 4 interactors

Molecular INTeraction database

More...MINTi		P23743

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000328405

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		P23743

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P23743, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1735
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...BMRBi		P23743

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P23743

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P23743

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini110 – 145EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini155 – 190EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini372 – 506DAGKcPROSITE-ProRule annotationAdd BLAST135

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the eukaryotic diacylglycerol kinase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri205 – 253Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri269 – 319Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1169, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT01040000241966

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_059202_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P23743

Identification of Orthologs from Complete Genome Data

More...OMAi		MQAPCTI

Database of Orthologous Groups

More...OrthoDBi		633642at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		P23743

TreeFam database of animal gene trees

More...TreeFami		TF313104

Family and domain databases

Conserved Domains Database

More...CDDi		cd00029, C1, 1 hit
cd00051, EFh, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.238.110, 1 hit
3.40.50.10330, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR017438, ATP-NAD_kinase_N
IPR029477, DAG_kinase_typeI_N
IPR037607, DGK
IPR038199, DGK_typeI_N_sf
IPR000756, Diacylglycerol_kin_accessory
IPR001206, Diacylglycerol_kinase_cat_dom
IPR011992, EF-hand-dom_pair
IPR018247, EF_Hand_1_Ca_BS
IPR002048, EF_hand_dom
IPR016064, NAD/diacylglycerol_kinase_sf
IPR002219, PE/DAG-bd

The PANTHER Classification System

More...PANTHERi		PTHR11255, PTHR11255, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00130, C1_1, 2 hits
PF14513, DAG_kinase_N, 2 hits
PF00609, DAGK_acc, 1 hit
PF00781, DAGK_cat, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00109, C1, 2 hits
SM00045, DAGKa, 1 hit
SM00046, DAGKc, 1 hit
SM00054, EFh, 2 hits

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF111331, SSF111331, 1 hit
SSF47473, SSF47473, 2 hits

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50146, DAGK, 1 hit
PS00018, EF_HAND_1, 2 hits
PS50222, EF_HAND_2, 2 hits
PS00479, ZF_DAG_PE_1, 2 hits
PS50081, ZF_DAG_PE_2, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P23743-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA KYVQGDAIGY 
        60         70         80         90        100
EGFQQFLKIY LEVDNVPRHL SLALFQSFET GHCLNETNVT KDVVCLNDVS 
       110        120        130        140        150
CYFSLLEGGR PEDKLEFTFK LYDTDRNGIL DSSEVDKIIL QMMRVAEYLD 
       160        170        180        190        200
WDVSELRPIL QEMMKEIDYD GSGSVSQAEW VRAGATTVPL LVLLGLEMTL 
       210        220        230        240        250
KDDGQHMWRP KRFPRPVYCN LCESSIGLGK QGLSCNLCKY TVHDQCAMKA 
       260        270        280        290        300
LPCEVSTYAK SRKDIGVQSH VWVRGGCESG RCDRCQKKIR IYHSLTGLHC 
       310        320        330        340        350
VWCHLEIHDD CLQAVGHECD CGLLRDHILP PSSIYPSVLA SGPDRKNSKT 
       360        370        380        390        400
SQKTMDDLNL STSEALRIDP VPNTHPLLVF VNPKSGGKQG QRVLWKFQYI 
       410        420        430        440        450
LNPRQVFNLL KDGPEIGLRL FKDVPDSRIL VCGGDGTVGW ILETIDKANL 
       460        470        480        490        500
PVLPPVAVLP LGTGNDLARC LRWGGGYEGQ NLAKILKDLE MSKVVHMDRW 
       510        520        530        540        550
SVEVIPQQTE EKSDPVPFQI INNYFSIGVD ASIAHRFHIM REKYPEKFNS 
       560        570        580        590        600
RMKNKLWYFE FATSESIFST CKKLEESLTV EICGKPLDLS NLSLEGIAVL 
       610        620        630        640        650
NIPSMHGGSN LWGDTRRPHG DIYGINQALG ATAKVITDPD ILKTCVPDLS 
       660        670        680        690        700
DKRLEVVGLE GAIEMGQIYT KLKNAGRRLA KCSEITFHTT KTLPMQIDGE 
       710        720        730 
PWMQTPCTIK ITHKNQMPML MGPPPRSTNF FGFLS
Length:735
Mass (Da):82,630
Last modified:December 15, 2009 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iACAA0AD186EE64A0
GO
Isoform 2 (identifier: P23743-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-118: FT → WS
     119-735: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.Curated
Show »
Length:118
Mass (Da):13,486
Checksum:iDC47336F5D8B584F
GO
Isoform 3 (identifier: P23743-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     237-247: LCKYTVHDQCA → RPITCVGARRL
     248-735: Missing.

Show »
Length:247
Mass (Da):28,094
Checksum:i8B502053A1906FB4
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V4E1G3V4E1_HUMAN
Diacylglycerol kinase
DGKA
545Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0YJH4H0YJH4_HUMAN
Diacylglycerol kinase
DGKA
202Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F8W1H7F8W1H7_HUMAN
Diacylglycerol kinase alpha
DGKA
147Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F8W1M3F8W1M3_HUMAN
Diacylglycerol kinase alpha
DGKA
133Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
G3V327G3V327_HUMAN
Diacylglycerol kinase alpha
DGKA
107Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0YJE7H0YJE7_HUMAN
Diacylglycerol kinase alpha
DGKA
97Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
C9JM35C9JM35_HUMAN
Diacylglycerol kinase alpha
DGKA
116Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F8VNZ9F8VNZ9_HUMAN
Diacylglycerol kinase alpha
DGKA
58Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
G3V312G3V312_HUMAN
Diacylglycerol kinase alpha
DGKA
94Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F8VWX8F8VWX8_HUMAN
Diacylglycerol kinase alpha
DGKA
21Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
There is more potential isoformShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti339L → P in AAC34804 (Ref. 2) Curated1
Sequence conflicti379V → L in AAC34802 (Ref. 2) Curated1
Sequence conflicti385S → W in AAC34802 (Ref. 2) Curated1
Sequence conflicti684E → G in AAC34803 (Ref. 2) Curated1
Sequence conflicti699G → V in CAA44396 (PubMed:2175712).Curated1
Sequence conflicti715N → K in AAC34803 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_031563538H → Y1 PublicationCorresponds to variant dbSNP:rs17852990Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_032212117 – 118FT → WS in isoform 2. 1 Publication2
Alternative sequenceiVSP_032213119 – 735Missing in isoform 2. 1 PublicationAdd BLAST617
Alternative sequenceiVSP_047702237 – 247LCKYTVHDQCA → RPITCVGARRL in isoform 3. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_047703248 – 735Missing in isoform 3. 1 PublicationAdd BLAST488

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X62535 mRNA Translation: CAA44396.1
AF064767 mRNA Translation: AAC34802.1
AF064768 mRNA Translation: AAC34803.1
AF064769 mRNA Translation: AAC34804.1
AF064771 mRNA Translation: AAC34806.1
AY930112 mRNA Translation: AAY20994.1
AC025162 Genomic DNA No translation available.
BC023523 mRNA Translation: AAH23523.1
BC031870 mRNA Translation: AAH31870.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS8896.1 [P23743-1]

Protein sequence database of the Protein Information Resource

More...PIRi		S12969

NCBI Reference Sequences

More...RefSeqi		NP_001336.2, NM_001345.4 [P23743-1]
NP_958852.1, NM_201444.2 [P23743-1]
NP_958853.1, NM_201445.1 [P23743-1]
NP_963848.1, NM_201554.1 [P23743-1]
XP_011536293.1, XM_011537991.2
XP_011536295.1, XM_011537993.2 [P23743-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000331886; ENSP00000328405; ENSG00000065357
ENST00000394147; ENSP00000377703; ENSG00000065357
ENST00000402956; ENSP00000385792; ENSG00000065357 [P23743-3]
ENST00000548549; ENSP00000448565; ENSG00000065357 [P23743-2]
ENST00000551156; ENSP00000450359; ENSG00000065357
ENST00000553084; ENSP00000446605; ENSG00000065357 [P23743-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		1606

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:1606

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000331886.10; ENSP00000328405.5; NM_001345.5; NP_001336.2

UCSC genome browser

More...UCSCi		uc001sij.4, human [P23743-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62535 mRNA Translation: CAA44396.1
AF064767 mRNA Translation: AAC34802.1
AF064768 mRNA Translation: AAC34803.1
AF064769 mRNA Translation: AAC34804.1
AF064771 mRNA Translation: AAC34806.1
AY930112 mRNA Translation: AAY20994.1
AC025162 Genomic DNA No translation available.
BC023523 mRNA Translation: AAH23523.1
BC031870 mRNA Translation: AAH31870.1
CCDSiCCDS8896.1 [P23743-1]
PIRiS12969
RefSeqiNP_001336.2, NM_001345.4 [P23743-1]
NP_958852.1, NM_201444.2 [P23743-1]
NP_958853.1, NM_201445.1 [P23743-1]
NP_963848.1, NM_201554.1 [P23743-1]
XP_011536293.1, XM_011537991.2
XP_011536295.1, XM_011537993.2 [P23743-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TUZNMR-A1-116[»]
6IIEX-ray2.14A107-197[»]
BMRBiP23743
SMRiP23743
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi107976, 8 interactors
IntActiP23743, 4 interactors
MINTiP23743
STRINGi9606.ENSP00000328405

Chemistry databases

BindingDBiP23743
ChEMBLiCHEMBL4105787
DrugBankiDB14001, alpha-Tocopherol succinate
DB00163, Vitamin E
SwissLipidsiSLP:000000555
SLP:000000739

PTM databases

GlyGeniP23743, 1 site, 1 O-linked glycan (1 site)
iPTMnetiP23743
MetOSiteiP23743
PhosphoSitePlusiP23743

Genetic variation databases

BioMutaiDGKA
DMDMi281185505

Proteomic databases

EPDiP23743
jPOSTiP23743
MassIVEiP23743
MaxQBiP23743
PaxDbiP23743
PeptideAtlasiP23743
PRIDEiP23743
ProteomicsDBi54146 [P23743-1]
54147 [P23743-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		27785, 438 antibodies from 31 providers

The DNASU plasmid repository

More...DNASUi		1606

Genome annotation databases

EnsembliENST00000331886; ENSP00000328405; ENSG00000065357
ENST00000394147; ENSP00000377703; ENSG00000065357
ENST00000402956; ENSP00000385792; ENSG00000065357 [P23743-3]
ENST00000548549; ENSP00000448565; ENSG00000065357 [P23743-2]
ENST00000551156; ENSP00000450359; ENSG00000065357
ENST00000553084; ENSP00000446605; ENSG00000065357 [P23743-2]
GeneIDi1606
KEGGihsa:1606
MANE-SelectiENST00000331886.10; ENSP00000328405.5; NM_001345.5; NP_001336.2
UCSCiuc001sij.4, human [P23743-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		1606
DisGeNETi1606

GeneCards: human genes, protein and diseases

More...GeneCardsi		DGKA
HGNCiHGNC:2849, DGKA
HPAiENSG00000065357, Tissue enhanced (esophagus, lymphoid tissue)
MIMi125855, gene
neXtProtiNX_P23743
OpenTargetsiENSG00000065357
PharmGKBiPA27310
VEuPathDBiHostDB:ENSG00000065357

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG1169, Eukaryota
GeneTreeiENSGT01040000241966
HOGENOMiCLU_059202_0_0_1
InParanoidiP23743
OMAiMQAPCTI
OrthoDBi633642at2759
PhylomeDBiP23743
TreeFamiTF313104

Enzyme and pathway databases

UniPathwayiUPA00230
BRENDAi2.7.1.107, 2681
PathwayCommonsiP23743
ReactomeiR-HSA-114508, Effects of PIP2 hydrolysis
SABIO-RKiP23743
SignaLinkiP23743
SIGNORiP23743

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		1606, 10 hits in 1045 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		DGKA, human
EvolutionaryTraceiP23743

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		DGKA

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		1606
PharosiP23743, Tbio

Protein Ontology

More...PROi		PR:P23743
RNActiP23743, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000065357, Expressed in small intestine Peyer's patch and 221 other tissues
ExpressionAtlasiP23743, baseline and differential
GenevisibleiP23743, HS

Family and domain databases

CDDicd00029, C1, 1 hit
cd00051, EFh, 1 hit
Gene3Di1.10.238.110, 1 hit
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438, ATP-NAD_kinase_N
IPR029477, DAG_kinase_typeI_N
IPR037607, DGK
IPR038199, DGK_typeI_N_sf
IPR000756, Diacylglycerol_kin_accessory
IPR001206, Diacylglycerol_kinase_cat_dom
IPR011992, EF-hand-dom_pair
IPR018247, EF_Hand_1_Ca_BS
IPR002048, EF_hand_dom
IPR016064, NAD/diacylglycerol_kinase_sf
IPR002219, PE/DAG-bd
PANTHERiPTHR11255, PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130, C1_1, 2 hits
PF14513, DAG_kinase_N, 2 hits
PF00609, DAGK_acc, 1 hit
PF00781, DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00109, C1, 2 hits
SM00045, DAGKa, 1 hit
SM00046, DAGKc, 1 hit
SM00054, EFh, 2 hits
SUPFAMiSSF111331, SSF111331, 1 hit
SSF47473, SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS50146, DAGK, 1 hit
PS00018, EF_HAND_1, 2 hits
PS50222, EF_HAND_2, 2 hits
PS00479, ZF_DAG_PE_1, 2 hits
PS50081, ZF_DAG_PE_2, 2 hits

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDGKA_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P23743
Secondary accession number(s): O75481
, O75482, O75483, O95217, Q3ZE25, Q8IZ56, Q8N5Q2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: December 15, 2009
Last modified: February 23, 2022
This is version 211 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families
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