UniProtKB - P23708 (NFYA_MOUSE)
Nuclear transcription factor Y subunit alpha
Nfya
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 295 – 320 | NFYA/HAP2-typePROSITE-ProRule annotationAdd BLAST | 26 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- sequence-specific DNA binding Source: MGI
- transcription cofactor binding Source: MGI
- transcription factor binding Source: MGI
GO - Biological processi
- positive regulation of stem cell proliferation Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of transcription from RNA polymerase II promoter in response to iron Source: GO_Central
- regulation of stem cell population maintenance Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- rhythmic process Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear transcription factor Y subunit alphaAlternative name(s): CAAT box DNA-binding protein subunit A Nuclear transcription factor Y subunit A Short name: NF-YA |
Gene namesi | Name:Nfya |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97316, Nfya |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- CCAAT-binding factor complex Source: MGI
- protein-DNA complex Source: MGI
- RNA polymerase II transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198769 | 1 – 346 | Nuclear transcription factor Y subunit alphaAdd BLAST | 346 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 325 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P23708 |
MaxQBi | P23708 |
PaxDbi | P23708 |
PRIDEi | P23708 |
PTM databases
iPTMneti | P23708 |
PhosphoSitePlusi | P23708 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000023994, Expressed in secondary oocyte and 292 other tissues |
ExpressionAtlasi | P23708, baseline and differential |
Genevisiblei | P23708, MM |
Interactioni
Subunit structurei
Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding (By similarity).
Interacts with SP1; the interaction is inhibited by glycosylation of SP1.
Interacts (via N-terminus) with ZHX2 (via homeobox domain).
Interacts with ZFX3.
Interacts with ZHX1 (By similarity).
By similarityBinary interactionsi
P23708
With | #Exp. | IntAct |
---|---|---|
SP1 [P08047] from Homo sapiens. | 18 | EBI-862337,EBI-298336 |
GO - Molecular functioni
- transcription cofactor binding Source: MGI
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201760, 17 interactors |
ComplexPortali | CPX-89, CCAAT-binding factor complex |
CORUMi | P23708 |
DIPi | DIP-2661N |
IntActi | P23708, 16 interactors |
MINTi | P23708 |
STRINGi | 10090.ENSMUSP00000043909 |
Miscellaneous databases
RNActi | P23708, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 265 – 288 | Subunit association domain (SAD)Add BLAST | 24 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 14 – 160 | Gln-richAdd BLAST | 147 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1561, Eukaryota |
GeneTreei | ENSGT00390000015714 |
InParanoidi | P23708 |
OMAi | MITQHIT |
PhylomeDBi | P23708 |
Family and domain databases
InterProi | View protein in InterPro IPR018362, CCAAT-binding_factor_CS IPR001289, NFYA |
PANTHERi | PTHR12632, PTHR12632, 1 hit |
Pfami | View protein in Pfam PF02045, CBFB_NFYA, 1 hit |
PRINTSi | PR00616, CCAATSUBUNTB |
SMARTi | View protein in SMART SM00521, CBF, 1 hit |
PROSITEi | View protein in PROSITE PS00686, NFYA_HAP2_1, 1 hit PS51152, NFYA_HAP2_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEQYTTNSNS STEQIVVQAG QIQQQQGGVT AVQLQTEAQV ASASGQQVQT
60 70 80 90 100
LQVVQGQPLM VQVSGGQLIT STGQPIMVQA VPGGQGQTIM QVPVSGTQGL
110 120 130 140 150
QQIQLVPPGQ IQIQGGQAVQ VQGQQGQTQQ IIIQQPQTAV TAGQTQTQQQ
160 170 180 190 200
IAVQGQQVAQ TAEGQTIVYQ PVNADGTILQ QVTVPVSGMI TIPAASLAGA
210 220 230 240 250
QIVQTGANTN TTSSGQGTVT VTLPVAGNVV NSGGMVMMVP GAGSVPAIQR
260 270 280 290 300
IPLPGAEMLE EEPLYVNAKQ YHRILKRRQA RAKLEAEGKI PKERRKYLHE
310 320 330 340
SRHRHAMARK RGEGGRFFSP KEKDSPHMQD PNQADEEAMT QIIRVS
The sequence of this isoform differs from the canonical sequence as follows:
26-53: Missing.
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ9DBV7 | Q9DBV7_MOUSE | Nfya protein | Nfya | 347 | Annotation score: | ||
E0CYF3 | E0CYF3_MOUSE | Nuclear transcription factor Y subu... | Nfya | 341 | Annotation score: | ||
A0A0R4J1Y8 | A0A0R4J1Y8_MOUSE | Nuclear transcription factor Y subu... | Nfya | 312 | Annotation score: | ||
F6TWX0 | F6TWX0_MOUSE | Nuclear transcription factor Y subu... | Nfya | 208 | Annotation score: | ||
F7DFJ2 | F7DFJ2_MOUSE | Nuclear transcription factor Y subu... | Nfya | 211 | Annotation score: | ||
E0CY40 | E0CY40_MOUSE | Nuclear transcription factor Y subu... | Nfya | 232 | Annotation score: | ||
F6TUY8 | F6TUY8_MOUSE | Nuclear transcription factor Y subu... | Nfya | 214 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 182 – 187 | Missing in AAA39816 (PubMed:1577736).Curated | 6 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000850 | 26 – 53 | Missing in isoform Short. CuratedAdd BLAST | 28 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55315 mRNA Translation: CAA39023.1 M86215 mRNA Translation: AAA39817.1 M86214 mRNA Translation: AAA39816.1 |
CCDSi | CCDS28867.1 [P23708-2] CCDS84321.1 [P23708-1] |
PIRi | A38245 C38245 D38245 E38245 |
RefSeqi | NP_001334330.1, NM_001347401.1 [P23708-1] NP_001334331.1, NM_001347402.1 NP_035043.1, NM_010913.2 [P23708-2] |
Genome annotation databases
Ensembli | ENSMUST00000078800; ENSMUSP00000077853; ENSMUSG00000023994 [P23708-1] ENSMUST00000162460; ENSMUSP00000123785; ENSMUSG00000023994 [P23708-2] ENSMUST00000228981; ENSMUSP00000155687; ENSMUSG00000115906 [P23708-1] ENSMUST00000230861; ENSMUSP00000155458; ENSMUSG00000115906 [P23708-2] |
GeneIDi | 18044 |
KEGGi | mmu:18044 |
UCSCi | uc008cxp.2, mouse [P23708-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55315 mRNA Translation: CAA39023.1 M86215 mRNA Translation: AAA39817.1 M86214 mRNA Translation: AAA39816.1 |
CCDSi | CCDS28867.1 [P23708-2] CCDS84321.1 [P23708-1] |
PIRi | A38245 C38245 D38245 E38245 |
RefSeqi | NP_001334330.1, NM_001347401.1 [P23708-1] NP_001334331.1, NM_001347402.1 NP_035043.1, NM_010913.2 [P23708-2] |
3D structure databases
SMRi | P23708 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201760, 17 interactors |
ComplexPortali | CPX-89, CCAAT-binding factor complex |
CORUMi | P23708 |
DIPi | DIP-2661N |
IntActi | P23708, 16 interactors |
MINTi | P23708 |
STRINGi | 10090.ENSMUSP00000043909 |
PTM databases
iPTMneti | P23708 |
PhosphoSitePlusi | P23708 |
Proteomic databases
jPOSTi | P23708 |
MaxQBi | P23708 |
PaxDbi | P23708 |
PRIDEi | P23708 |
Protocols and materials databases
Antibodypediai | 3982, 212 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000078800; ENSMUSP00000077853; ENSMUSG00000023994 [P23708-1] ENSMUST00000162460; ENSMUSP00000123785; ENSMUSG00000023994 [P23708-2] ENSMUST00000228981; ENSMUSP00000155687; ENSMUSG00000115906 [P23708-1] ENSMUST00000230861; ENSMUSP00000155458; ENSMUSG00000115906 [P23708-2] |
GeneIDi | 18044 |
KEGGi | mmu:18044 |
UCSCi | uc008cxp.2, mouse [P23708-1] |
Organism-specific databases
CTDi | 4800 |
MGIi | MGI:97316, Nfya |
Phylogenomic databases
eggNOGi | KOG1561, Eukaryota |
GeneTreei | ENSGT00390000015714 |
InParanoidi | P23708 |
OMAi | MITQHIT |
PhylomeDBi | P23708 |
Miscellaneous databases
BioGRID-ORCSi | 18044, 5 hits in 17 CRISPR screens |
ChiTaRSi | Nfya, mouse |
PROi | PR:P23708 |
RNActi | P23708, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023994, Expressed in secondary oocyte and 292 other tissues |
ExpressionAtlasi | P23708, baseline and differential |
Genevisiblei | P23708, MM |
Family and domain databases
InterProi | View protein in InterPro IPR018362, CCAAT-binding_factor_CS IPR001289, NFYA |
PANTHERi | PTHR12632, PTHR12632, 1 hit |
Pfami | View protein in Pfam PF02045, CBFB_NFYA, 1 hit |
PRINTSi | PR00616, CCAATSUBUNTB |
SMARTi | View protein in SMART SM00521, CBF, 1 hit |
PROSITEi | View protein in PROSITE PS00686, NFYA_HAP2_1, 1 hit PS51152, NFYA_HAP2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NFYA_MOUSE | |
Accessioni | P23708Primary (citable) accession number: P23708 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | May 1, 1992 | |
Last modified: | February 10, 2021 | |
This is version 181 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families