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Protein

Troponin I, cardiac muscle

Gene

Tnni3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei81Involved in TNI-TNT interactions1
Sitei98Involved in TNI-TNT interactions1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi138 – 149By similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Muscle protein
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-390522 Striated Muscle Contraction
R-RNO-5578775 Ion homeostasis

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin I, cardiac muscle
Alternative name(s):
Cardiac troponin I
Gene namesi
Name:Tnni3
Synonyms:Ctni, Tni
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi62052 Tnni3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001861552 – 211Troponin I, cardiac muscleAdd BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei5PhosphoserineBy similarity1
Modified residuei6PhosphoserineCombined sources1
Modified residuei23Phosphoserine; by PKA and PKD/PRKD1By similarity1
Modified residuei24Phosphoserine; by PKA and PKD/PRKD1By similarity1
Modified residuei27PhosphotyrosineBy similarity1
Modified residuei32Phosphothreonine; by STK4/MST1By similarity1
Modified residuei43Phosphoserine; by PKC/PRKCEBy similarity1
Modified residuei45Phosphoserine; by PKC/PRKCEBy similarity1
Modified residuei52Phosphothreonine; by STK4/MST1By similarity1
Modified residuei78PhosphoserineBy similarity1
Modified residuei79PhosphothreonineBy similarity1
Modified residuei130Phosphothreonine; by STK4/MST1By similarity1
Modified residuei144Phosphothreonine; by STK4/MST1By similarity1
Modified residuei151Phosphoserine; by PAK3By similarity1
Modified residuei167PhosphoserineBy similarity1
Modified residuei200PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylated at Ser-43 and Ser-45 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP23693
PRIDEiP23693

PTM databases

iPTMnetiP23693
PhosphoSitePlusiP23693

Expressioni

Gene expression databases

BgeeiENSRNOG00000018250 Expressed in 8 organ(s), highest expression level in heart
GenevisibleiP23693 RN

Interactioni

Subunit structurei

Interacts with TRIM63 (By similarity). Binds to actin and tropomyosin. Interacts with STK4/MST1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PDE4DIPQ5VU43-112EBI-10817583,EBI-10769071From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247923, 2 interactors
IntActiP23693, 1 interactor
STRINGi10116.ENSRNOP00000024640

Structurei

3D structure databases

ProteinModelPortaliP23693
SMRiP23693
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 80Involved in binding TNCAdd BLAST48
Regioni130 – 151Involved in binding TNC and actinAdd BLAST22

Sequence similaritiesi

Belongs to the troponin I family.Curated

Phylogenomic databases

eggNOGiKOG3977 Eukaryota
ENOG410Y9IX LUCA
GeneTreeiENSGT00390000002746
HOGENOMiHOG000293300
HOVERGENiHBG052737
InParanoidiP23693
KOiK12044
OMAiHTRVDKV
OrthoDBiEOG091G0NOD
PhylomeDBiP23693
TreeFamiTF313374

Family and domain databases

Gene3Di1.20.5.350, 1 hit
InterProiView protein in InterPro
IPR001978 Troponin
IPR021666 Troponin-I_N
IPR038077 Troponin_sf
PfamiView protein in Pfam
PF00992 Troponin, 1 hit
PF11636 Troponin-I_N, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23693-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADESSDAAG EPQPAPAPVR RRSSANYRAY ATEPHAKKKS KISASRKLQL
60 70 80 90 100
KTLMLQIAKQ EMEREAEERR GEKGRVLSTR CQPLVLDGLG FEELQDLCRQ
110 120 130 140 150
LHARVDKVDE ERYDVEAKVT KNITEIADLT QKIYDLRGKF KRPTLRRVRI
160 170 180 190 200
SADAMMQALL GTRAKESLDL RAHLKQVKKE DIEKENREVG DWRKNIDALS
210
GMEGRKKKFE G
Length:211
Mass (Da):24,160
Last modified:January 23, 2007 - v2
Checksum:i17586B0FDB682B4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8A → S in AAA42294 (PubMed:1886137).Curated1
Sequence conflicti182I → T in AAA42294 (PubMed:1886137).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57679 mRNA Translation: AAA63504.1
X58499 mRNA Translation: CAA41402.1
M92074 mRNA Translation: AAA42294.1
U77354 Genomic DNA Translation: AAB52234.1
DQ062462 mRNA Translation: AAY63993.1
PIRiA60124
I56441
RefSeqiNP_058840.1, NM_017144.2
UniGeneiRn.64141

Genome annotation databases

EnsembliENSRNOT00000024640; ENSRNOP00000024640; ENSRNOG00000018250
GeneIDi29248
KEGGirno:29248
UCSCiRGD:62052 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57679 mRNA Translation: AAA63504.1
X58499 mRNA Translation: CAA41402.1
M92074 mRNA Translation: AAA42294.1
U77354 Genomic DNA Translation: AAB52234.1
DQ062462 mRNA Translation: AAY63993.1
PIRiA60124
I56441
RefSeqiNP_058840.1, NM_017144.2
UniGeneiRn.64141

3D structure databases

ProteinModelPortaliP23693
SMRiP23693
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247923, 2 interactors
IntActiP23693, 1 interactor
STRINGi10116.ENSRNOP00000024640

PTM databases

iPTMnetiP23693
PhosphoSitePlusiP23693

Proteomic databases

PaxDbiP23693
PRIDEiP23693

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024640; ENSRNOP00000024640; ENSRNOG00000018250
GeneIDi29248
KEGGirno:29248
UCSCiRGD:62052 rat

Organism-specific databases

CTDi7137
RGDi62052 Tnni3

Phylogenomic databases

eggNOGiKOG3977 Eukaryota
ENOG410Y9IX LUCA
GeneTreeiENSGT00390000002746
HOGENOMiHOG000293300
HOVERGENiHBG052737
InParanoidiP23693
KOiK12044
OMAiHTRVDKV
OrthoDBiEOG091G0NOD
PhylomeDBiP23693
TreeFamiTF313374

Enzyme and pathway databases

ReactomeiR-RNO-390522 Striated Muscle Contraction
R-RNO-5578775 Ion homeostasis

Miscellaneous databases

PROiPR:P23693

Gene expression databases

BgeeiENSRNOG00000018250 Expressed in 8 organ(s), highest expression level in heart
GenevisibleiP23693 RN

Family and domain databases

Gene3Di1.20.5.350, 1 hit
InterProiView protein in InterPro
IPR001978 Troponin
IPR021666 Troponin-I_N
IPR038077 Troponin_sf
PfamiView protein in Pfam
PF00992 Troponin, 1 hit
PF11636 Troponin-I_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTNNI3_RAT
AccessioniPrimary (citable) accession number: P23693
Secondary accession number(s): Q4PP23
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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