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UniProtKB - P23657 (T2P2_PROHU)
Protein
Type II restriction enzyme PvuII
Gene
pvuIIR
Organism
Proteus hauseri
Status
Functioni
A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-CAGCTG-3' and cleaves after G-3.
1 PublicationCatalytic activityi
- Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. EC:3.1.21.4
Cofactori
Mg2+Note: Binds 2 magnesium ions per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 58 | Magnesium 1By similarity | 1 | |
Metal bindingi | 58 | Magnesium 2By similarity | 1 | |
Metal bindingi | 68 | Magnesium 1By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Endonuclease, Hydrolase, Nuclease |
Biological process | Restriction system |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.1.21.4, 14542 |
Names & Taxonomyi
Protein namesi | Recommended name: Type II restriction enzyme PvuII1 Publication (EC:3.1.21.4)Short name: R.PvuII Alternative name(s): Endonuclease PvuII Type-2 restriction enzyme PvuII |
Gene namesi | Name:pvuIIR |
Organismi | Proteus hauseri |
Taxonomic identifieri | 183417 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Morganellaceae › Proteus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000077356 | 1 – 157 | Type II restriction enzyme PvuIIAdd BLAST | 157 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23657 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23657 |
Family & Domainsi
Family and domain databases
Gene3Di | 3.40.210.10, 1 hit |
InterProi | View protein in InterPro IPR038402, PvuII_sf IPR011335, Restrct_endonuc-II-like IPR015306, Restrct_endonuc_II_PvuII |
Pfami | View protein in Pfam PF09225, Endonuc-PvuII, 1 hit |
SUPFAMi | SSF52980, SSF52980, 1 hit |
i Sequence
Sequence statusi: Complete.
P23657-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSHPDLNKLL ELWPHIQEYQ DLALKHGIND IFQDNGGKLL QVLLITGLTV
60 70 80 90 100
LPGREGNDAV DNAGQEYELK SINIDLTKGF STHHHMNPVI IAKYRQVPWI
110 120 130 140 150
FAIYRGIAIE AIYRLEPKDL EFYYDKWERK WYSDGHKDIN NPKIPVKYVM
EHGTKIY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52681 Genomic DNA Translation: CAA36904.1 AF305615 Genomic DNA Translation: AAA96334.1 |
PIRi | S12163 |
RefSeqi | WP_010904455.1, NZ_PGWU01000024.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52681 Genomic DNA Translation: CAA36904.1 AF305615 Genomic DNA Translation: AAA96334.1 |
PIRi | S12163 |
RefSeqi | WP_010904455.1, NZ_PGWU01000024.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EYU | X-ray | 1.78 | A/B | 1-157 | [»] | |
1F0O | X-ray | 2.50 | A/B | 1-157 | [»] | |
1H56 | X-ray | 3.00 | A/B | 2-157 | [»] | |
1K0Z | X-ray | 2.05 | A/B | 2-157 | [»] | |
1NI0 | X-ray | 2.50 | A/B/C | 1-157 | [»] | |
1PVI | X-ray | 2.60 | A/B | 1-157 | [»] | |
1PVU | X-ray | 2.40 | A/B | 2-157 | [»] | |
2PVI | X-ray | 1.76 | A/B | 1-157 | [»] | |
3KSK | X-ray | 2.35 | A/B | 2-157 | [»] | |
3PVI | X-ray | 1.59 | A/B | 1-157 | [»] | |
SMRi | P23657 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
BRENDAi | 3.1.21.4, 14542 |
Miscellaneous databases
EvolutionaryTracei | P23657 |
PROi | PR:P23657 |
Family and domain databases
Gene3Di | 3.40.210.10, 1 hit |
InterProi | View protein in InterPro IPR038402, PvuII_sf IPR011335, Restrct_endonuc-II-like IPR015306, Restrct_endonuc_II_PvuII |
Pfami | View protein in Pfam PF09225, Endonuc-PvuII, 1 hit |
SUPFAMi | SSF52980, SSF52980, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | T2P2_PROHU | |
Accessioni | P23657Primary (citable) accession number: P23657 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | February 23, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references