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Protein

Proteasome subunit alpha type-2

Gene

PRE8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.

Miscellaneous

Present with 7060 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • proteasomal ubiquitin-independent protein catabolic process Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciYEAST:G3O-32677-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-187577 SCF(Skp2)-mediated degradation of p27/p21
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-6798695 Neutrophil degranulation
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-2 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit Y7
Multicatalytic endopeptidase complex subunit Y7
Proteasome component Y7
Proteinase YSCE subunit 7
Gene namesi
Name:PRE8
Synonyms:PRS4
Ordered Locus Names:YML092C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML092C
SGDiS000004557 PRE8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001240901 – 250Proteasome subunit alpha type-2Add BLAST250

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP23639
PaxDbiP23639
PRIDEiP23639

PTM databases

iPTMnetiP23639

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35051, 151 interactors
ComplexPortaliCPX-2262 26S Proteasome complex
DIPiDIP-2822N
IntActiP23639, 36 interactors
MINTiP23639
STRINGi4932.YML092C

Chemistry databases

BindingDBiP23639

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 10Combined sources4
Turni14 – 16Combined sources3
Helixi19 – 29Combined sources11
Beta strandi34 – 39Combined sources6
Beta strandi42 – 48Combined sources7
Beta strandi54 – 56Combined sources3
Helixi58 – 60Combined sources3
Beta strandi63 – 68Combined sources6
Beta strandi71 – 77Combined sources7
Helixi79 – 95Combined sources17
Helixi98 – 101Combined sources4
Helixi107 – 120Combined sources14
Turni121 – 123Combined sources3
Beta strandi132 – 140Combined sources9
Turni141 – 143Combined sources3
Beta strandi144 – 150Combined sources7
Beta strandi156 – 165Combined sources10
Helixi168 – 178Combined sources11
Helixi185 – 199Combined sources15
Turni206 – 208Combined sources3
Beta strandi209 – 214Combined sources6
Helixi219 – 221Combined sources3
Beta strandi223 – 226Combined sources4
Beta strandi234 – 237Combined sources4
Helixi240 – 247Combined sources8

3D structure databases

ProteinModelPortaliP23639
SMRiP23639
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23639

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074870
HOGENOMiHOG000091085
InParanoidiP23639
KOiK02726
OMAiFAWKASA
OrthoDBiEOG092C47D8

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR000426 Proteasome_asu_N
IPR001353 Proteasome_sua/b
IPR034644 Proteasome_subunit_alpha2
PANTHERiPTHR11599:SF16 PTHR11599:SF16, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequencei

Sequence statusi: Complete.

P23639-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRYSFSLT TFSPSGKLGQ IDYALTAVKQ GVTSLGIKAT NGVVIATEKK
60 70 80 90 100
SSSPLAMSET LSKVSLLTPD IGAVYSGMGP DYRVLVDKSR KVAHTSYKRI
110 120 130 140 150
YGEYPPTKLL VSEVAKIMQE ATQSGGVRPF GVSLLIAGHD EFNGFSLYQV
160 170 180 190 200
DPSGSYFPWK ATAIGKGSVA AKTFLEKRWN DELELEDAIH IALLTLKESV
210 220 230 240 250
EGEFNGDTIE LAIIGDENPD LLGYTGIPTD KGPRFRKLTS QEINDRLEAL
Length:250
Mass (Da):27,162
Last modified:November 1, 1991 - v1
Checksum:i12D7DFB1AA10E9FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56731 Genomic DNA Translation: CAA40055.1
Z46660 Genomic DNA Translation: CAA86646.1
AY557762 Genomic DNA Translation: AAS56088.1
BK006946 Genomic DNA Translation: DAA09807.1
PIRiS12938 SNBYY7
RefSeqiNP_013618.1, NM_001182451.1

Genome annotation databases

EnsemblFungiiYML092C; YML092C; YML092C
GeneIDi854882
KEGGisce:YML092C

Similar proteinsi

Entry informationi

Entry nameiPSA2_YEAST
AccessioniPrimary (citable) accession number: P23639
Secondary accession number(s): D6W0J3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 18, 2018
This is version 198 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

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