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Protein

Transcription elongation factor SPT6

Gene

SPT6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression. Essential for viability.4 Publications

Miscellaneous

Present with 8890 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA binding Source: InterPro
  • histone binding Source: SGD
  • nucleosome binding Source: SGD
  • transcription antitermination factor activity, DNA binding Source: SGD
  • transcription factor activity, core RNA polymerase II binding Source: SGD

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30823-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor SPT6
Alternative name(s):
Chromatin elongation factor SPT6
Gene namesi
Name:SPT6
Synonyms:CRE2, SSN20
Ordered Locus Names:YGR116W
ORF Names:G6169
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR116W
SGDiS000003348 SPT6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721731 – 1451Transcription elongation factor SPT6Add BLAST1451

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei94PhosphoserineCombined sources1
Modified residuei134PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei148PhosphoserineCombined sources1
Modified residuei155PhosphoserineCombined sources1
Modified residuei206PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23615
PaxDbiP23615
PRIDEiP23615

PTM databases

iPTMnetiP23615

Interactioni

Subunit structurei

Interacts with CTR9.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33361, 159 interactors
DIPiDIP-2630N
IntActiP23615, 21 interactors
MINTiP23615
STRINGi4932.YGR116W

Structurei

Secondary structure

11451
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP23615
SMRiP23615
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23615

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1257 – 1354SH2PROSITE-ProRule annotationAdd BLAST98

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi8 – 12Nuclear localization signalSequence analysis5
Motifi77 – 85Nuclear localization signalSequence analysis9
Motifi120 – 125Nuclear localization signalSequence analysis6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 484Asp/Glu-rich (acidic)Add BLAST484

Sequence similaritiesi

Belongs to the SPT6 family.Curated

Keywords - Domaini

SH2 domain

Phylogenomic databases

GeneTreeiENSGT00510000047446
HOGENOMiHOG000193710
InParanoidiP23615
KOiK11292
OMAiEADWIYR
OrthoDBiEOG092C04AI

Family and domain databases

CDDicd09928 SH2_Cterm_SPT6_like, 1 hit
cd09918 SH2_Nterm_SPT6_like, 1 hit
Gene3Di1.10.10.650, 1 hit
1.10.3500.10, 2 hits
3.30.420.140, 2 hits
3.30.505.10, 2 hits
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR010994 RuvA_2-like
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR028083 Spt6_acidic_N_dom
IPR027999 Spt6_dom
IPR032706 Spt6_HHH
IPR028088 Spt6_HTH_DNA-bd_dom
IPR035420 Spt6_SH2
IPR035018 Spt6_SH2_C
IPR035019 Spt6_SH2_N
IPR028231 Spt6_YqgF
IPR023323 Tex-like_dom_sf
IPR023319 Tex-like_HTH_dom_sf
IPR017072 TF_Spt6
IPR006641 YqgF/RNaseH-like_dom
IPR037027 YqgF/RNaseH-like_dom_sf
PANTHERiPTHR10145 PTHR10145, 1 hit
PfamiView protein in Pfam
PF14878 DLD, 1 hit
PF14635 HHH_7, 1 hit
PF14641 HTH_44, 1 hit
PF14633 SH2_2, 1 hit
PF14632 SPT6_acidic, 1 hit
PF14639 YqgF, 1 hit
PIRSFiPIRSF036947 Spt6, 1 hit
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00732 YqgFc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF53098 SSF53098, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit

Sequencei

Sequence statusi: Complete.

P23615-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEETGDSKLV PRDEEEIVND NDETKAPSEE EEGEDVFDSS EEDEDIDEDE
60 70 80 90 100
DEARKVQEGF IVNDDDENED PGTSISKKRR KHKRREREED DRLSEDDLDL
110 120 130 140 150
LMENAGVERT KASSSSGKFK RLKRVGDEGN AAESESDNVA ASRQDSTSKL
160 170 180 190 200
EDFFSEDEEE EESGLRNGRN NEYGRDEEDH ENRNRTADKG GILDELDDFI
210 220 230 240 250
EDDEFSDEDD ETRQRRIQEK KLLREQSIKQ PTQITGLSSD KIDEMYDIFG
260 270 280 290 300
DGHDYDWALE IENEELENGN DNNEAEEEEI DEETGAIKST KKKISLQDIY
310 320 330 340 350
DLEDLKKNLM TEGDMKIRKT DIPERYQELR AGITDYGNMS SEDQELERNW
360 370 380 390 400
IAEKISVDKN FDANYDLTEF KEAIGNAIKF ITKENLEVPF IYAYRRNYIS
410 420 430 440 450
SREKDGFLLT EDDLWDIVSL DIEFHSLVNK KDYVQRFYAE LHIDDPIVTE
460 470 480 490 500
YFKNQNTASI AELNSLQDIY DYLEFKYANE INEMFINHTG KTGKKHLKNS
510 520 530 540 550
SYEKFKASPL YQAVSDIGIS AEDVGENISS QHQIHPPVDH PSSKPVEVIE
560 570 580 590 600
SILNANSGDL QVFTSNTKLA IDTVQKYYSL ELSKNTKIRE KVRSDFSKYY
610 620 630 640 650
LADVVLTAKG KKEIQKGSLY EDIKYAINRT PMHFRRDPDV FLKMVEAESL
660 670 680 690 700
NLLSVKLHMS SQAQYIEHLF QIALETTNTS DIAIEWNNFR KLAFNQAMDK
710 720 730 740 750
IFQDISQEVK DNLTKNCQKL VAKTVRHKFM TKLDQAPFIP NVRDPKIPKI
760 770 780 790 800
LSLTCGQGRF GADAIIAVYV NRKGDFIRDY KIVDNPFDKT NPEKFEDTLD
810 820 830 840 850
NIIQSCQPNA IGINGPNPKT QKFYKRLQEV LHKKQIVDSR GHTIPIIYVE
860 870 880 890 900
DEVAIRYQNS ERAAQEFPNK PPLVKYCIAL ARYMHSPLLE YANLTSEEVR
910 920 930 940 950
SLSIHPHQNL LSSEQLSWAL ETAFVDIVNL VSVEVNKATD NNYYASALKY
960 970 980 990 1000
ISGFGKRKAI DFLQSLQRLN EPLLARQQLI THNILHKTIF MNSAGFLYIS
1010 1020 1030 1040 1050
WNEKRQKYED LEHDQLDSTR IHPEDYHLAT KVAADALEYD PDTIAEKEEQ
1060 1070 1080 1090 1100
GTMSEFIELL REDPDRRAKL ESLNLESYAE ELEKNTGLRK LNNLNTIVLE
1110 1120 1130 1140 1150
LLDGFEELRN DFHPLQGDEI FQSLTGESEK TFFKGSIIPV RVERFWHNDI
1160 1170 1180 1190 1200
ICTTNSEVEC VVNAQRHAGA QLRRPANEIY EIGKTYPAKV IYIDYANITA
1210 1220 1230 1240 1250
EVSLLDHDVK QQYVPISYSK DPSIWDLKQE LEDAEEERKL MMAEARAKRT
1260 1270 1280 1290 1300
HRVINHPYYF PFNGRQAEDY LRSKERGEFV IRQSSRGDDH LVITWKLDKD
1310 1320 1330 1340 1350
LFQHIDIQEL EKENPLALGK VLIVDNQKYN DLDQIIVEYL QNKVRLLNEM
1360 1370 1380 1390 1400
TSSEKFKSGT KKDVVKFIED YSRVNPNKSV YYFSLNHDNP GWFYLMFKIN
1410 1420 1430 1440 1450
ANSKLYTWNV KLTNTGYFLV NYNYPSVIQL CNGFKTLLKS NSSKNRMNNY

R
Length:1,451
Mass (Da):168,291
Last modified:November 1, 1991 - v1
Checksum:i0BE9922A59BD0483
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34391 Genomic DNA Translation: AAA35086.1
Z72899 Genomic DNA Translation: CAA97124.1
Z72902 Genomic DNA Translation: CAA97127.1
BK006941 Genomic DNA Translation: DAA08208.1
PIRiA36468
RefSeqiNP_011631.1, NM_001181245.1

Genome annotation databases

EnsemblFungiiYGR116W; YGR116W; YGR116W
GeneIDi853011
KEGGisce:YGR116W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34391 Genomic DNA Translation: AAA35086.1
Z72899 Genomic DNA Translation: CAA97124.1
Z72902 Genomic DNA Translation: CAA97127.1
BK006941 Genomic DNA Translation: DAA08208.1
PIRiA36468
RefSeqiNP_011631.1, NM_001181245.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L3TNMR-A1250-1440[»]
3OAKX-ray2.15C/D239-263[»]
3PSFX-ray2.59A235-1259[»]
3PSIX-ray3.30A239-1451[»]
3PSJX-ray2.70A1247-1451[»]
3PSKX-ray2.10A/B/C/D1247-1451[»]
5VKLX-ray2.20A1247-1451[»]
5VKOX-ray1.80A1247-1451[»]
ProteinModelPortaliP23615
SMRiP23615
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33361, 159 interactors
DIPiDIP-2630N
IntActiP23615, 21 interactors
MINTiP23615
STRINGi4932.YGR116W

PTM databases

iPTMnetiP23615

Proteomic databases

MaxQBiP23615
PaxDbiP23615
PRIDEiP23615

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR116W; YGR116W; YGR116W
GeneIDi853011
KEGGisce:YGR116W

Organism-specific databases

EuPathDBiFungiDB:YGR116W
SGDiS000003348 SPT6

Phylogenomic databases

GeneTreeiENSGT00510000047446
HOGENOMiHOG000193710
InParanoidiP23615
KOiK11292
OMAiEADWIYR
OrthoDBiEOG092C04AI

Enzyme and pathway databases

BioCyciYEAST:G3O-30823-MONOMER

Miscellaneous databases

EvolutionaryTraceiP23615
PROiPR:P23615

Family and domain databases

CDDicd09928 SH2_Cterm_SPT6_like, 1 hit
cd09918 SH2_Nterm_SPT6_like, 1 hit
Gene3Di1.10.10.650, 1 hit
1.10.3500.10, 2 hits
3.30.420.140, 2 hits
3.30.505.10, 2 hits
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR010994 RuvA_2-like
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR028083 Spt6_acidic_N_dom
IPR027999 Spt6_dom
IPR032706 Spt6_HHH
IPR028088 Spt6_HTH_DNA-bd_dom
IPR035420 Spt6_SH2
IPR035018 Spt6_SH2_C
IPR035019 Spt6_SH2_N
IPR028231 Spt6_YqgF
IPR023323 Tex-like_dom_sf
IPR023319 Tex-like_HTH_dom_sf
IPR017072 TF_Spt6
IPR006641 YqgF/RNaseH-like_dom
IPR037027 YqgF/RNaseH-like_dom_sf
PANTHERiPTHR10145 PTHR10145, 1 hit
PfamiView protein in Pfam
PF14878 DLD, 1 hit
PF14635 HHH_7, 1 hit
PF14641 HTH_44, 1 hit
PF14633 SH2_2, 1 hit
PF14632 SPT6_acidic, 1 hit
PF14639 YqgF, 1 hit
PIRSFiPIRSF036947 Spt6, 1 hit
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00732 YqgFc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF53098 SSF53098, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPT6_YEAST
AccessioniPrimary (citable) accession number: P23615
Secondary accession number(s): D6VUP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: September 12, 2018
This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
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