UniProtKB - P23611 (IRF8_MOUSE)
Protein
Interferon regulatory factor 8
Gene
Irf8
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor that specifically binds to the upstream regulatory region of type I interferon (IFN) and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) (PubMed:2111015, PubMed:12393690). Can both act as a transcriptional activator or repressor (PubMed:2111015). Plays a negative regulatory role in cells of the immune system (PubMed:2111015). Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (PubMed:12393690, PubMed:22992524). Required for the development of plasmacytoid dendritic cells (pDCs), which produce most of the type I IFN in response to viral infection (PubMed:12461077, PubMed:12393690, PubMed:12538667, PubMed:23382217). Positively regulates macroautophagy in dendritic cells (By similarity).By similarity6 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 7 – 114 | IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST | 108 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- autophagy Source: UniProtKB-KW
- cellular response to interferon-gamma Source: UniProtKB
- cellular response to lipopolysaccharide Source: MGI
- defense response to bacterium Source: MGI
- defense response to protozoan Source: MGI
- dendritic cell differentiation Source: UniProtKB
- follicular B cell differentiation Source: ARUK-UCL
- germinal center B cell differentiation Source: ARUK-UCL
- immune response Source: UniProtKB
- immune system process Source: GO_Central
- myeloid cell differentiation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- phagocytosis Source: MGI
- plasmacytoid dendritic cell differentiation Source: UniProtKB
- positive regulation of interferon-gamma production Source: MGI
- positive regulation of interleukin-12 production Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: ARUK-UCL
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of type I interferon production Source: UniProtKB
- response to bacterium Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Autophagy, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Interferon regulatory factor 81 PublicationShort name: IRF-81 Publication Alternative name(s): Interferon consensus sequence-binding protein1 Publication Short name: ICSBP1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96395, Irf8 |
Subcellular locationi
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Mice display an absence of interferon (IFN)-producing cells and show impaired IFN production in response to viral infection (PubMed:12461077). Complete absence of plasmacytoid dendritic cells (pDCs) and conventional CD8+-expressing dendritic cells (cDCs) (PubMed:12393690, PubMed:12538667, PubMed:23382217). Mice display reduced autoimmunity (PubMed:23382217).4 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154565 | 1 – 424 | Interferon regulatory factor 8Add BLAST | 424 |
Post-translational modificationi
Ubiquitinated (PubMed:17579016). Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes (PubMed:17579016). Ubiquitination leads to its degradation by the proteasome (By similarity).By similarity1 Publication
Sumoylated with SUMO3. Desumoylated by SENP1.By similarity
Keywords - PTMi
Ubl conjugationProteomic databases
EPDi | P23611 |
PaxDbi | P23611 |
PeptideAtlasi | P23611 |
PRIDEi | P23611 |
ProteomicsDBi | 267007 |
PTM databases
iPTMneti | P23611 |
PhosphoSitePlusi | P23611 |
SwissPalmi | P23611 |
Expressioni
Tissue specificityi
Mainly expressed in lymphoid tissues (PubMed:2111015). Predominantly expressed in CD8+-expressing dendritic cells (PubMed:12393690).2 Publications
Inductioni
By interferon gamma.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000041515, Expressed in spleen and 215 other tissues |
ExpressionAtlasi | P23611, baseline and differential |
Genevisiblei | P23611, MM |
Interactioni
Subunit structurei
Interacts with COPS2 (By similarity).
Interacts (via C-terminus) with TRIM21 (via C-terminus).
Interacts with the BATF-JUNB heterodimer.
Interacts with BATF (via bZIP domain); the interaction is direct.
By similarity2 PublicationsProtein-protein interaction databases
BioGRIDi | 200504, 6 interactors |
CORUMi | P23611 |
IntActi | P23611, 1 interactor |
STRINGi | 10090.ENSMUSP00000040245 |
Miscellaneous databases
RNActi | P23611, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the IRF family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | ENOG502QT9P, Eukaryota |
GeneTreei | ENSGT00940000158140 |
HOGENOMi | CLU_031544_1_1_1 |
InParanoidi | P23611 |
OMAi | HSNKQGI |
OrthoDBi | 648909at2759 |
PhylomeDBi | P23611 |
TreeFami | TF328512 |
Family and domain databases
CDDi | cd00103, IRF, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 2.60.200.10, 1 hit |
InterProi | View protein in InterPro IPR019817, Interferon_reg_fac_CS IPR001346, Interferon_reg_fact_DNA-bd_dom IPR019471, Interferon_reg_factor-3 IPR044400, IRF8 IPR017855, SMAD-like_dom_sf IPR008984, SMAD_FHA_dom_sf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11949, PTHR11949, 1 hit PTHR11949:SF7, PTHR11949:SF7, 1 hit |
Pfami | View protein in Pfam PF00605, IRF, 1 hit PF10401, IRF-3, 1 hit |
PRINTSi | PR00267, INTFRNREGFCT |
SMARTi | View protein in SMART SM00348, IRF, 1 hit SM01243, IRF-3, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF49879, SSF49879, 1 hit |
PROSITEi | View protein in PROSITE PS00601, IRF_1, 1 hit PS51507, IRF_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P23611-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCDRNGGRRL RQWLIEQIDS SMYPGLIWEN DEKTMFRIPW KHAGKQDYNQ
60 70 80 90 100
EVDASIFKAW AVFKGKFKEG DKAEPATWKT RLRCALNKSP DFEEVTDRSQ
110 120 130 140 150
LDISEPYKVY RIVPEEEQKC KLGVAPAGCM SEVPEMECGR SEIEELIKEP
160 170 180 190 200
SVDEYMGMTK RSPSPPEACR SQILPDWWVQ QPSAGLPLVT GYAAYDTHHS
210 220 230 240 250
AFSQMVISFY YGGKLVGQAT TTCLEGCRLS LSQPGLPKLY GPDGLEPVCF
260 270 280 290 300
PTADTIPSER QRQVTRKLFG HLERGVLLHS NRKGVFVKRL CQGRVFCSGN
310 320 330 340 350
AVVCKGRPNK LERDEVVQVF DTNQFIRELQ QFYATQSRLP DSRVVLCFGE
360 370 380 390 400
EFPDTVPLRS KLILVQVEQL YARQLVEEAG KSCGAGSLMP ALEEPQPDQA
410 420
FRMFPDICTS HQRPFFRENQ QITV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE0CZE4 | E0CZE4_MOUSE | Interferon regulatory factor 8 | Irf8 | 151 | Annotation score: | ||
F7C1A3 | F7C1A3_MOUSE | Interferon regulatory factor 8 | Irf8 | 333 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32489 mRNA Translation: AAA37878.1 AK018533 mRNA Translation: BAB31258.1 BC005450 mRNA Translation: AAH05450.1 |
CCDSi | CCDS22721.1 |
PIRi | A35861 |
RefSeqi | NP_001288740.1, NM_001301811.1 NP_032346.1, NM_008320.4 |
Genome annotation databases
Ensembli | ENSMUST00000047737; ENSMUSP00000040245; ENSMUSG00000041515 ENSMUST00000162001; ENSMUSP00000125029; ENSMUSG00000041515 |
GeneIDi | 15900 |
KEGGi | mmu:15900 |
UCSCi | uc009nrk.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32489 mRNA Translation: AAA37878.1 AK018533 mRNA Translation: BAB31258.1 BC005450 mRNA Translation: AAH05450.1 |
CCDSi | CCDS22721.1 |
PIRi | A35861 |
RefSeqi | NP_001288740.1, NM_001301811.1 NP_032346.1, NM_008320.4 |
3D structure databases
SMRi | P23611 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200504, 6 interactors |
CORUMi | P23611 |
IntActi | P23611, 1 interactor |
STRINGi | 10090.ENSMUSP00000040245 |
PTM databases
iPTMneti | P23611 |
PhosphoSitePlusi | P23611 |
SwissPalmi | P23611 |
Proteomic databases
EPDi | P23611 |
PaxDbi | P23611 |
PeptideAtlasi | P23611 |
PRIDEi | P23611 |
ProteomicsDBi | 267007 |
Protocols and materials databases
Antibodypediai | 1058, 454 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000047737; ENSMUSP00000040245; ENSMUSG00000041515 ENSMUST00000162001; ENSMUSP00000125029; ENSMUSG00000041515 |
GeneIDi | 15900 |
KEGGi | mmu:15900 |
UCSCi | uc009nrk.2, mouse |
Organism-specific databases
CTDi | 3394 |
MGIi | MGI:96395, Irf8 |
Phylogenomic databases
eggNOGi | ENOG502QT9P, Eukaryota |
GeneTreei | ENSGT00940000158140 |
HOGENOMi | CLU_031544_1_1_1 |
InParanoidi | P23611 |
OMAi | HSNKQGI |
OrthoDBi | 648909at2759 |
PhylomeDBi | P23611 |
TreeFami | TF328512 |
Miscellaneous databases
BioGRID-ORCSi | 15900, 0 hits in 52 CRISPR screens |
ChiTaRSi | Irf8, mouse |
PROi | PR:P23611 |
RNActi | P23611, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041515, Expressed in spleen and 215 other tissues |
ExpressionAtlasi | P23611, baseline and differential |
Genevisiblei | P23611, MM |
Family and domain databases
CDDi | cd00103, IRF, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 2.60.200.10, 1 hit |
InterProi | View protein in InterPro IPR019817, Interferon_reg_fac_CS IPR001346, Interferon_reg_fact_DNA-bd_dom IPR019471, Interferon_reg_factor-3 IPR044400, IRF8 IPR017855, SMAD-like_dom_sf IPR008984, SMAD_FHA_dom_sf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11949, PTHR11949, 1 hit PTHR11949:SF7, PTHR11949:SF7, 1 hit |
Pfami | View protein in Pfam PF00605, IRF, 1 hit PF10401, IRF-3, 1 hit |
PRINTSi | PR00267, INTFRNREGFCT |
SMARTi | View protein in SMART SM00348, IRF, 1 hit SM01243, IRF-3, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF49879, SSF49879, 1 hit |
PROSITEi | View protein in PROSITE PS00601, IRF_1, 1 hit PS51507, IRF_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IRF8_MOUSE | |
Accessioni | P23611Primary (citable) accession number: P23611 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families