UniProtKB - P23589 (CAH5A_MOUSE)
Protein
Carbonic anhydrase 5A, mitochondrial
Gene
Ca5a
Organism
Mus musculus (Mouse)
Status
Functioni
Reversible hydration of carbon dioxide. Low activity.
Catalytic activityi
- EC:4.2.1.1
Cofactori
Zn2+3 Publications
Activity regulationi
Inhibited by acetazolamide.By similarity
pH dependencei
Optimum pH is 7-8.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 124 | Zinc; catalytic3 Publications | 1 | |
Metal bindingi | 126 | Zinc; catalytic3 Publications | 1 | |
Metal bindingi | 149 | Zinc; catalytic3 Publications | 1 |
GO - Molecular functioni
- carbonate dehydratase activity Source: MGI
- hydro-lyase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- gluconeogenesis Source: MGI
- one-carbon metabolic process Source: GO_Central
Keywordsi
Molecular function | Lyase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-1475029, Reversible hydration of carbon dioxide |
Names & Taxonomyi
Protein namesi | Recommended name: Carbonic anhydrase 5A, mitochondrial (EC:4.2.1.1)Alternative name(s): Carbonate dehydratase VA Short name: CA Y Carbonic anhydrase VA Short name: CA-VA |
Gene namesi | Name:Ca5a Synonyms:Ca5, Car5, Car5a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101946, Car5a |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrion Source: MGI
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 94 | Y → A: Normal activity. 2 Publications | 1 | |
Mutagenesisi | 94 | Y → H: Normal activity. Enhanced proton transfer due to removal of the steric hindrance of F-95; when associated with A-95. 2 Publications | 1 | |
Mutagenesisi | 95 | F → A: Normal activity. Enhanced proton transfer; when associated with H-94 or C-161. 3 Publications | 1 | |
Mutagenesisi | 121 | K → C: Normal activity. 1 Publication | 1 | |
Mutagenesisi | 161 | Y → A: Normal activity. 3 Publications | 1 | |
Mutagenesisi | 161 | Y → C: Normal activity. Enhanced proton transfer; when associated with A-95. 3 Publications | 1 | |
Mutagenesisi | 161 | Y → H: Normal activity. 3 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 29 | Mitochondrion1 PublicationAdd BLAST | 29 | |
ChainiPRO_0000004235 | 30 – 299 | Carbonic anhydrase 5A, mitochondrialAdd BLAST | 270 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 66 | N6-succinyllysineCombined sources | 1 |
Proteomic databases
jPOSTi | P23589 |
MaxQBi | P23589 |
PaxDbi | P23589 |
PeptideAtlasi | P23589 |
PRIDEi | P23589 |
PTM databases
iPTMneti | P23589 |
PhosphoSitePlusi | P23589 |
Expressioni
Tissue specificityi
Liver.
Gene expression databases
Bgeei | ENSMUSG00000025317, Expressed in liver and 64 other tissues |
Genevisiblei | P23589, MM |
Interactioni
Protein-protein interaction databases
IntActi | P23589, 1 interactor |
MINTi | P23589 |
STRINGi | 10090.ENSMUSP00000060457 |
Chemistry databases
BindingDBi | P23589 |
Miscellaneous databases
RNActi | P23589, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23589 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23589 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 290 | Alpha-carbonic anhydrasePROSITE-ProRule annotationAdd BLAST | 261 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 229 – 230 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the alpha-carbonic anhydrase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0382, Eukaryota |
GeneTreei | ENSGT00940000162066 |
HOGENOMi | CLU_039326_2_1_1 |
InParanoidi | P23589 |
OMAi | NAWKTSA |
OrthoDBi | 1377476at2759 |
PhylomeDBi | P23589 |
TreeFami | TF316425 |
Family and domain databases
Gene3Di | 3.10.200.10, 1 hit |
InterProi | View protein in InterPro IPR001148, CA_dom IPR036398, CA_dom_sf IPR023561, Carbonic_anhydrase_a-class IPR018338, Carbonic_anhydrase_a-class_CS |
PANTHERi | PTHR18952, PTHR18952, 1 hit |
Pfami | View protein in Pfam PF00194, Carb_anhydrase, 1 hit |
SMARTi | View protein in SMART SM01057, Carb_anhydrase, 1 hit |
SUPFAMi | SSF51069, SSF51069, 1 hit |
PROSITEi | View protein in PROSITE PS00162, ALPHA_CA_1, 1 hit PS51144, ALPHA_CA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P23589-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRRDPRKPL AILRHVGLLC ATGPQRWRFQ HSCAEEHSNC ARHPLWTGPV
60 70 80 90 100
SSAEGTRQSP INIQWKDSVY DPQLAPLRVS YDAASCRYLW NTGYFFQVEF
110 120 130 140 150
DDSCEDSGIS GGPLGNHYRL KQFHFHWGAT DEWGSEHAVD GHTYPAELHL
160 170 180 190 200
VHWNSTKYEN YKKASVGENG LAVIGVFLKL GAHHQALQKL VDVLPEVRHK
210 220 230 240 250
DTQVAMGPFD PSCLLPACRD YWTYPGSLTT PPLAESVTWI VQKTPVEVSP
260 270 280 290
SQLSTFRTLL FSGRGEEEDV MVNNYRPLQP LRDRKLRSSF RLDRTKMRS
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 151 – 153 | VHW → FM in strain: BIO-HTT. | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X51971 mRNA Translation: CAA36233.1 BC030174 mRNA Translation: AAH30174.1 |
CCDSi | CCDS22731.1 |
PIRi | S12579 |
RefSeqi | NP_031634.2, NM_007608.2 |
Genome annotation databases
Ensembli | ENSMUST00000057653; ENSMUSP00000060457; ENSMUSG00000025317 |
GeneIDi | 12352 |
KEGGi | mmu:12352 |
UCSCi | uc009nsf.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X51971 mRNA Translation: CAA36233.1 BC030174 mRNA Translation: AAH30174.1 |
CCDSi | CCDS22731.1 |
PIRi | S12579 |
RefSeqi | NP_031634.2, NM_007608.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DMX | X-ray | 2.45 | A/B | 53-299 | [»] | |
1DMY | X-ray | 2.45 | A/B | 53-299 | [»] | |
1KEQ | X-ray | 1.88 | A/B | 53-299 | [»] | |
1URT | X-ray | 2.80 | A | 52-299 | [»] | |
SMRi | P23589 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P23589, 1 interactor |
MINTi | P23589 |
STRINGi | 10090.ENSMUSP00000060457 |
Chemistry databases
BindingDBi | P23589 |
PTM databases
iPTMneti | P23589 |
PhosphoSitePlusi | P23589 |
Proteomic databases
jPOSTi | P23589 |
MaxQBi | P23589 |
PaxDbi | P23589 |
PeptideAtlasi | P23589 |
PRIDEi | P23589 |
Protocols and materials databases
Antibodypediai | 55888, 138 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000057653; ENSMUSP00000060457; ENSMUSG00000025317 |
GeneIDi | 12352 |
KEGGi | mmu:12352 |
UCSCi | uc009nsf.2, mouse |
Organism-specific databases
CTDi | 12352 |
MGIi | MGI:101946, Car5a |
Phylogenomic databases
eggNOGi | KOG0382, Eukaryota |
GeneTreei | ENSGT00940000162066 |
HOGENOMi | CLU_039326_2_1_1 |
InParanoidi | P23589 |
OMAi | NAWKTSA |
OrthoDBi | 1377476at2759 |
PhylomeDBi | P23589 |
TreeFami | TF316425 |
Enzyme and pathway databases
Reactomei | R-MMU-1475029, Reversible hydration of carbon dioxide |
Miscellaneous databases
BioGRID-ORCSi | 12352, 0 hits in 17 CRISPR screens |
ChiTaRSi | Car5a, mouse |
EvolutionaryTracei | P23589 |
PROi | PR:P23589 |
RNActi | P23589, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025317, Expressed in liver and 64 other tissues |
Genevisiblei | P23589, MM |
Family and domain databases
Gene3Di | 3.10.200.10, 1 hit |
InterProi | View protein in InterPro IPR001148, CA_dom IPR036398, CA_dom_sf IPR023561, Carbonic_anhydrase_a-class IPR018338, Carbonic_anhydrase_a-class_CS |
PANTHERi | PTHR18952, PTHR18952, 1 hit |
Pfami | View protein in Pfam PF00194, Carb_anhydrase, 1 hit |
SMARTi | View protein in SMART SM01057, Carb_anhydrase, 1 hit |
SUPFAMi | SSF51069, SSF51069, 1 hit |
PROSITEi | View protein in PROSITE PS00162, ALPHA_CA_1, 1 hit PS51144, ALPHA_CA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAH5A_MOUSE | |
Accessioni | P23589Primary (citable) accession number: P23589 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families