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Protein

Cyclin-dependent kinase 1

Gene

Cdk1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle (PubMed:2120044, PubMed:15581871). It is required in higher cells for entry into S-phase and mitosis (PubMed:2120044, PubMed:15581871, PubMed:29746464). In embryos, promotes the release of Rif1 from chromatin during mid-blastula transition (PubMed:29746464). p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II (PubMed:2120044).3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: FlyBase
  • protein kinase activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase
  • RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  • asymmetric neuroblast division Source: FlyBase
  • DNA repair Source: GO_Central
  • G1/S transition of mitotic cell cycle Source: FlyBase
  • G2/M transition of mitotic cell cycle Source: FlyBase
  • germarium-derived cystoblast division Source: FlyBase
  • histone phosphorylation Source: GO_Central
  • male meiotic nuclear division Source: FlyBase
  • microtubule cytoskeleton organization Source: GO_Central
  • mitotic G2/M transition checkpoint Source: FlyBase
  • mitotic G2 DNA damage checkpoint Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of gene expression Source: GO_Central
  • regulation of meiotic cell cycle Source: GO_Central
  • regulation of protein localization Source: FlyBase
  • spermatogonial cell division Source: FlyBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-110056 MAPK3 (ERK1) activation
R-DME-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-DME-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-DME-176408 Regulation of APC/C activators between G1/S and early anaphase
R-DME-176412 Phosphorylation of the APC/C
R-DME-2500257 Resolution of Sister Chromatid Cohesion
R-DME-3301854 Nuclear Pore Complex (NPC) Disassembly
R-DME-4419969 Depolymerisation of the Nuclear Lamina
R-DME-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-DME-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-DME-69478 G2/M DNA replication checkpoint
R-DME-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
R-DME-8878166 Transcriptional regulation by RUNX2
SignaLinkiP23572

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1
Alternative name(s):
Cell division control protein 2 homolog
Cell division protein kinase 1
p34 protein kinase
Gene namesi
Name:Cdk1Imported
Synonyms:cdc2Imported
ORF Names:CG5363Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0004106 Cdk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43G → D in cdc2-E1-4; larval-pupal lethal with some adult escapers. 1 Publication1
Mutagenesisi145A → V in cdc2-216A; larval-pupal lethal. 2 Publications1
Mutagenesisi148G → R in cdc2-D57; embryonic or larval-pupal lethal. 2 Publications1
Mutagenesisi176L → Q in cdc2-E10; larval-pupal lethal. 1 Publication1
Mutagenesisi196E → K in cdc2-E1-24; larval-pupal lethal with some adult escapers. 2 Publications1
Mutagenesisi206G → D in cdc2-E1-23; larval-pupal lethal. 2 Publications1
Mutagenesisi242P → S in cdc2-E1-9; larval-pupal lethal. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857431 – 297Cyclin-dependent kinase 1Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14Phosphothreonine1 Publication1
Modified residuei15Phosphotyrosine1 Publication1
Modified residuei160Phosphotyrosine1 Publication1
Modified residuei161Phosphothreonine; by CAKSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP23572
PRIDEiP23572

PTM databases

iPTMnetiP23572

Expressioni

Developmental stagei

Expressed both maternally and zygotically (PubMed:2120044, PubMed:8405984). High levels of expression when mitosis is elevated; highest levels of expression are in early embryos with levels decreasing during embryogenesis and remaining low throughout most of larval development, and expression levels are also increased in unfertilized eggs and adult females (PubMed:2120044).2 Publications

Gene expression databases

BgeeiFBgn0004106
ExpressionAtlasiP23572 baseline and differential
GenevisibleiP23572 DM

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Component of the Frs-CycA-Cdk1 complex composed of Cdk1, CycA and Z600 (PubMed:17431409).1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi60495, 47 interactors
DIPiDIP-649N
IntActiP23572, 38 interactors
MINTiP23572
STRINGi7227.FBpp0079641

Structurei

3D structure databases

ProteinModelPortaliP23572
SMRiP23572
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 287Protein kinasePROSITE-ProRule annotationAdd BLAST284

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0594 Eukaryota
ENOG410XPP3 LUCA
GeneTreeiENSGT00910000144030
InParanoidiP23572
KOiK02087
OMAiEMMLVYD
OrthoDBiEOG091G0CH0
PhylomeDBiP23572

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P23572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDFEKIEKI GEGTYGVVYK GRNRLTGQIV AMKKIRLESD DEGVPSTAIR
60 70 80 90 100
EISLLKELKH ENIVCLEDVL MEENRIYLIF EFLSMDLKKY MDSLPVDKHM
110 120 130 140 150
ESELVRSYLY QITSAILFCH RRRVLHRDLK PQNLLIDKSG LIKVADFGLG
160 170 180 190 200
RSFGIPVRIY THEIVTLWYR APEVLLGSPR YSCPVDIWSI GCIFAEMATR
210 220 230 240 250
KPLFQGDSEI DQLFRMFRIL KTPTEDIWPG VTSLPDYKNT FPCWSTNQLT
260 270 280 290
NQLKNLDANG IDLIQKMLIY DPVHRISAKD ILEHPYFNGF QSGLVRN
Length:297
Mass (Da):34,439
Last modified:November 1, 1991 - v1
Checksum:i952CF136D8AB64C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57485 mRNA Translation: CAA40723.1
X57496 mRNA Translation: CAA40733.1
S66801 Genomic DNA Translation: AAP13986.1
S66804 Genomic DNA Translation: AAP13987.1
S66805 Genomic DNA Translation: AAP13988.1
S66807 Genomic DNA Translation: AAP13989.1
S66810 Genomic DNA Translation: AAP13990.1
AM294319 Genomic DNA Translation: CAL26249.1
AM294320 Genomic DNA Translation: CAL26250.1
AM294321 Genomic DNA Translation: CAL26251.1
AM294322 Genomic DNA Translation: CAL26252.1
AM294323 Genomic DNA Translation: CAL26253.1
AM294324 Genomic DNA Translation: CAL26254.1
AM294325 Genomic DNA Translation: CAL26255.1
AM294326 Genomic DNA Translation: CAL26256.1
AM294327 Genomic DNA Translation: CAL26257.1
AM294328 Genomic DNA Translation: CAL26258.1
AM294329 Genomic DNA Translation: CAL26259.1
AE014134 Genomic DNA Translation: AAF52932.1
AY061450 mRNA Translation: AAL28998.1
PIRiS12009
RefSeqiNP_476797.1, NM_057449.4
UniGeneiDm.3187

Genome annotation databases

EnsemblMetazoaiFBtr0080051; FBpp0079641; FBgn0004106
GeneIDi34411
KEGGidme:Dmel_CG5363

Similar proteinsi

Entry informationi

Entry nameiCDK1_DROME
AccessioniPrimary (citable) accession number: P23572
Secondary accession number(s): A0ANT1
, Q86FT5, Q86FT6, Q86FT7, Q86FT8, Q86FT9, Q9TX68, Q9TX69, Q9TX70, Q9TX71, Q9TX72, Q9TX73, Q9TX74, Q9VKX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 18, 2018
This is version 196 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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