UniProtKB - P23565 (AINX_RAT)
Protein
Alpha-internexin
Gene
Ina
Organism
Rattus norvegicus (Rat)
Status
Functioni
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges (By similarity). May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (By similarity).By similarity
GO - Molecular functioni
- protein-containing complex binding Source: RGD
- structural constituent of postsynaptic actin cytoskeleton Source: RGD
- structural constituent of postsynaptic intermediate filament cytoskeleton Source: RGD
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- cellular response to leukemia inhibitory factor Source: RGD
- intermediate filament cytoskeleton organization Source: RGD
- nervous system development Source: UniProtKB-KW
- neurofilament cytoskeleton organization Source: RGD
- tissue regeneration Source: RGD
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation, Neurogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-internexinShort name: Alpha-Inx |
Gene namesi | Name:Ina Synonyms:Inexa |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2911, Ina |
Subcellular locationi
Cytoskeleton
- neurofilament Source: RGD
- postsynaptic intermediate filament cytoskeleton Source: SynGO
Other locations
- cytoplasmic ribonucleoprotein granule Source: RGD
- glutamatergic synapse Source: SynGO
- postsynapse Source: RGD
- Schaffer collateral - CA1 synapse Source: RGD
Keywords - Cellular componenti
Intermediate filamentPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063785 | 1 – 505 | Alpha-internexinAdd BLAST | 505 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 72 | PhosphoserineBy similarity | 1 | |
Modified residuei | 219 | PhosphoserineBy similarity | 1 | |
Modified residuei | 290 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 335 | PhosphoserineCombined sources | 1 | |
Modified residuei | 474 | PhosphoserineCombined sources | 1 | |
Modified residuei | 502 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
O-glycosylated.By similarity
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | P23565 |
PaxDbi | P23565 |
PRIDEi | P23565 |
2D gel databases
World-2DPAGEi | 0004:P23565 |
PTM databases
iPTMneti | P23565 |
PhosphoSitePlusi | P23565 |
Expressioni
Tissue specificityi
Expressed in the dorsal root ganglion neurons (at protein level).1 Publication
Developmental stagei
Levels of this protein reach a maximum at embryonic day 16 and decline into adulthood.
Interactioni
Subunit structurei
Binary interactionsi
P23565
With | #Exp. | IntAct |
---|---|---|
Nsmf [Q9EPI6] | 5 | EBI-6899875,EBI-6899705 |
Protein-protein interaction databases
BioGRIDi | 246662, 7 interactors |
IntActi | P23565, 4 interactors |
MINTi | P23565 |
STRINGi | 10116.ENSRNOP00000027417 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 94 – 407 | IF rodPROSITE-ProRule annotationAdd BLAST | 314 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 87 | HeadAdd BLAST | 87 | |
Regioni | 88 – 129 | Coil 1AAdd BLAST | 42 | |
Regioni | 130 – 142 | Linker 1Add BLAST | 13 | |
Regioni | 143 – 238 | Coil 1BAdd BLAST | 96 | |
Regioni | 239 – 262 | Linker 2Add BLAST | 24 | |
Regioni | 263 – 408 | Coil 2Add BLAST | 146 | |
Regioni | 409 – 505 | TailAdd BLAST | 97 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 449 – 458 | Poly-Glu | 10 |
Sequence similaritiesi
Belongs to the intermediate filament family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502RAU0, Eukaryota |
InParanoidi | P23565 |
PhylomeDBi | P23565 |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit |
InterProi | View protein in InterPro IPR027703, Alpha-Inx IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR006821, Intermed_filament_DNA-bd |
PANTHERi | PTHR45652:SF18, PTHR45652:SF18, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF04732, Filament_head, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P23565-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFGSEHYLC SASSYRKVFG DGSRLSARLS GPGASGSFRS QSLSRSNVAS
60 70 80 90 100
TAACSSASSL GLGLAYRRLP ASDGLDLSQA AARTNEYKII RTNEKEQLQG
110 120 130 140 150
LNDRFAVFIE KVHQLETQNR ALEAELAALR QRHAEPSRVG ELFQRELREL
160 170 180 190 200
RAQLEEASSA RAQALLERDG LAEEVQRLRA RCEEESRGRE GAERALKAQQ
210 220 230 240 250
RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA TLQASSQAAA
260 270 280 290 300
EVDVAVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR
310 320 330 340 350
STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE
360 370 380 390 400
VAGYQDSIGQ LESDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL
410 420 430 440 450
EGEETRFSTS GLSISGLNPL PNPSYLLPPR ILSSTTSKVS SAGLSLKKEE
460 470 480 490 500
EEEEEEEEGA SKEVTKKTSK VGESFEETLE ETVVSTKKTE KSTIEEITTS
SSQKM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V8Q2 | G3V8Q2_RAT | Alpha-internexin | Ina Inexa, rCG_57469 | 506 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 152 – 153 | AQ → GE in CAA36264 (PubMed:2311576).Curated | 2 | |
Sequence conflicti | 239 – 242 | Missing AA sequence (PubMed:2311576).Curated | 4 | |
Sequence conflicti | 458 | Missing AA sequence (PubMed:2311576).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52017 mRNA Translation: CAA36264.1 M73049 Genomic DNA Translation: AAA41444.1 |
PIRi | A41023 |
Genome annotation databases
UCSCi | RGD:2911, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52017 mRNA Translation: CAA36264.1 M73049 Genomic DNA Translation: AAA41444.1 |
PIRi | A41023 |
3D structure databases
SMRi | P23565 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246662, 7 interactors |
IntActi | P23565, 4 interactors |
MINTi | P23565 |
STRINGi | 10116.ENSRNOP00000027417 |
PTM databases
iPTMneti | P23565 |
PhosphoSitePlusi | P23565 |
2D gel databases
World-2DPAGEi | 0004:P23565 |
Proteomic databases
jPOSTi | P23565 |
PaxDbi | P23565 |
PRIDEi | P23565 |
Genome annotation databases
UCSCi | RGD:2911, rat |
Organism-specific databases
RGDi | 2911, Ina |
Phylogenomic databases
eggNOGi | ENOG502RAU0, Eukaryota |
InParanoidi | P23565 |
PhylomeDBi | P23565 |
Miscellaneous databases
PROi | PR:P23565 |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit |
InterProi | View protein in InterPro IPR027703, Alpha-Inx IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR006821, Intermed_filament_DNA-bd |
PANTHERi | PTHR45652:SF18, PTHR45652:SF18, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF04732, Filament_head, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AINX_RAT | |
Accessioni | P23565Primary (citable) accession number: P23565 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | October 1, 1996 | |
Last modified: | October 7, 2020 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families