UniProtKB - P23562 (B3AT_RAT)
Band 3 anion transport protein
Slc4a1
Functioni
Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
By similarityGO - Molecular functioni
- actin binding Source: RGD
- anion:anion antiporter activity Source: UniProtKB
- ankyrin binding Source: RGD
- bicarbonate transmembrane transporter activity Source: UniProtKB
- chloride transmembrane transporter activity Source: UniProtKB
- enzyme binding Source: RGD
- hemoglobin binding Source: RGD
- inorganic anion exchanger activity Source: UniProtKB
- protein-containing complex binding Source: RGD
- protein C-terminus binding Source: RGD
- protein homodimerization activity Source: RGD
- transmembrane transporter activity Source: GO_Central
GO - Biological processi
- anion transport Source: UniProtKB
- bicarbonate transport Source: UniProtKB
- blood coagulation Source: RGD
- chloride transport Source: UniProtKB
- circadian rhythm Source: RGD
- erythrocyte development Source: RGD
- ion homeostasis Source: GO_Central
- negative regulation of glycolytic process through fructose-6-phosphate Source: RGD
- negative regulation of oxidoreductase activity Source: RGD
- negative regulation of urine volume Source: RGD
- pH elevation Source: RGD
- plasma membrane phospholipid scrambling Source: RGD
- positive regulation of T cell proliferation Source: RGD
- protein localization to plasma membrane Source: RGD
- regulation of intracellular pH Source: GO_Central
- response to acidic pH Source: RGD
- response to activity Source: RGD
- response to alkaline pH Source: RGD
- response to arsenic-containing substance Source: RGD
- response to carbon dioxide Source: RGD
- response to hydrogen peroxide Source: RGD
- response to nutrient levels Source: RGD
- response to water deprivation Source: RGD
- transmembrane transport Source: GO_Central
Keywordsi
Biological process | Anion exchange, Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-1237044, Erythrocytes take up carbon dioxide and release oxygen R-RNO-1247673, Erythrocytes take up oxygen and release carbon dioxide R-RNO-425381, Bicarbonate transporters |
Names & Taxonomyi
Protein namesi | Recommended name: Band 3 anion transport proteinAlternative name(s): Anion exchange protein 1 Short name: AE 1 Short name: Anion exchanger 1 Solute carrier family 4 member 1 CD_antigen: CD233 |
Gene namesi | Name:Slc4a1 Synonyms:Ae1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3710, Slc4a1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
- Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.By similarity
Cytoskeleton
- cortical cytoskeleton Source: UniProtKB
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB
- cytoplasmic side of plasma membrane Source: RGD
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: RGD
Other locations
- cell surface Source: RGD
- intercalated disc Source: RGD
- membrane Source: RGD
- Z disc Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 420 | CytoplasmicBy similarityAdd BLAST | 420 | |
Transmembranei | 421 – 444 | Helical; Name=1By similarityAdd BLAST | 24 | |
Topological domaini | 445 – 452 | ExtracellularBy similarity | 8 | |
Transmembranei | 453 – 473 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 474 – 476 | CytoplasmicBy similarity | 3 | |
Transmembranei | 477 – 493 | Discontinuously helical; Name=3By similarityAdd BLAST | 17 | |
Topological domaini | 494 – 502 | ExtracellularBy similarity | 9 | |
Transmembranei | 503 – 523 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 524 – 535 | CytoplasmicBy similarityAdd BLAST | 12 | |
Transmembranei | 536 – 558 | Helical; Name=5By similarityAdd BLAST | 23 | |
Topological domaini | 559 – 586 | ExtracellularBy similarityAdd BLAST | 28 | |
Transmembranei | 587 – 607 | Helical; Name=6By similarityAdd BLAST | 21 | |
Topological domaini | 608 – 618 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 619 – 639 | Helical; Name=7By similarityAdd BLAST | 21 | |
Topological domaini | 640 – 679 | ExtracellularBy similarityAdd BLAST | 40 | |
Transmembranei | 680 – 700 | Helical; Name=8By similarityAdd BLAST | 21 | |
Topological domaini | 701 – 716 | CytoplasmicBy similarityAdd BLAST | 16 | |
Transmembranei | 717 – 735 | Helical; Name=9By similarityAdd BLAST | 19 | |
Transmembranei | 736 – 753 | Discontinuously helical; Name=10By similarityAdd BLAST | 18 | |
Topological domaini | 754 – 776 | CytoplasmicBy similarityAdd BLAST | 23 | |
Transmembranei | 777 – 797 | Helical; Name=11By similarityAdd BLAST | 21 | |
Transmembranei | 798 – 816 | Helical; Name=12By similarityAdd BLAST | 19 | |
Topological domaini | 817 – 854 | CytoplasmicBy similarityAdd BLAST | 38 | |
Intramembranei | 855 – 885 | Discontinuously helicalBy similarityAdd BLAST | 31 | |
Topological domaini | 886 – 927 | CytoplasmicBy similarityAdd BLAST | 42 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079211 | 1 – 927 | Band 3 anion transport proteinAdd BLAST | 927 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 18 | PhosphoserineCombined sources | 1 | |
Modified residuei | 31 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 56 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 199 | PhosphoserineCombined sources | 1 | |
Modified residuei | 222 | PhosphoserineCombined sources | 1 | |
Modified residuei | 372 | PhosphotyrosineBy similarity | 1 | |
Glycosylationi | 658 | N-linked (GlcNAc...) asparagineCurated | 1 | |
Lipidationi | 859 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 920 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
Acetylation, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | P23562 |
PeptideAtlasi | P23562 |
PTM databases
GlyGeni | P23562, 1 site |
iPTMneti | P23562 |
PhosphoSitePlusi | P23562 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically.
Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association.
Interacts with STOM.
Isoform 2 interacts with TMEM139.
By similarityGO - Molecular functioni
- actin binding Source: RGD
- ankyrin binding Source: RGD
- enzyme binding Source: RGD
- hemoglobin binding Source: RGD
- protein C-terminus binding Source: RGD
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
IntActi | P23562, 1 interactor |
STRINGi | 10116.ENSRNOP00000028445 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 69 – 303 | GlobularBy similarityAdd BLAST | 235 | |
Regioni | 190 – 199 | Interaction with ANK1By similarity | 10 | |
Regioni | 317 – 370 | Dimerization armBy similarityAdd BLAST | 54 | |
Regioni | 367 – 390 | DisorderedSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1172, Eukaryota |
InParanoidi | P23562 |
OrthoDBi | 265068at2759 |
PhylomeDBi | P23562 |
Family and domain databases
Gene3Di | 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR001717, Anion_exchange IPR002977, Anion_exchange_1 IPR018241, Anion_exchange_CS IPR013769, Band3_cytoplasmic_dom IPR011531, HCO3_transpt-like_TM_dom IPR003020, HCO3_transpt_euk IPR016152, PTrfase/Anion_transptr |
PANTHERi | PTHR11453, PTHR11453, 1 hit |
Pfami | View protein in Pfam PF07565, Band_3_cyto, 1 hit PF00955, HCO3_cotransp, 2 hits |
PRINTSi | PR00165, ANIONEXCHNGR PR01187, ANIONEXHNGR1 PR01231, HCO3TRNSPORT |
SUPFAMi | SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00834, ae, 1 hit |
PROSITEi | View protein in PROSITE PS00219, ANION_EXCHANGER_1, 1 hit PS00220, ANION_EXCHANGER_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGDMQDHEKV LEIPDRDSEE ELEHVIEQIA YRDLDIPVTE MQESEALPTE
60 70 80 90 100
QTATDYIPTS TSTSHPSSSQ VYVELQELMM DQRNQELQWV EAAHWIGLEE
110 120 130 140 150
NLREDGVWGR PHLSYLTFWS LLELQKVFSK GTFLLDLAET SLAGVANKLL
160 170 180 190 200
DSFIYEDQIR PQDRDELLRA LLLKRSHAED LKDLEGVKPA VLTRSGAPSE
210 220 230 240 250
PLLPHQPSLE TKLYCAQAEG GSEEPSPSGI LKIPPNSETT LVLVGRASFL
260 270 280 290 300
VKPVLGFVRL KEAVPLEDLV LPEPVSFLLV LLGPEAPHID YTQLGRAAAT
310 320 330 340 350
LMTERVFRVT ASLAQSRGEL LSSLDSFLDC SLVLPPTEAP SEKALLNLVP
360 370 380 390 400
VQKELLRKRY LPRPAKPDPN LYEALDGGKE GPGDEDDPLR RTGRIFGGLI
410 420 430 440 450
RDIRRRYPYY LSDITDALSP QVLAAVIFIY FAALSPAVTF GGLLGEKTRN
460 470 480 490 500
LMGVSELLIS TAVQGILFAL LGAQPLLVLG FSGPLLVFEE AFYSFCESNN
510 520 530 540 550
LEYIVGRAWI GFWLILLVVL VVAFEGSFLV QYISRYTQEI FSFLISLIFI
560 570 580 590 600
YETFSKLIKI FQDYPLQESY APVVMKPKPQ GPVPNTALLS LVLMVGTFLL
610 620 630 640 650
AMMLRKFKNS TYFPGKLRRV IGDFGVPISI LIMVLVDTFI KNTYTQKLSV
660 670 680 690 700
PDGLKVSNSS ARGWVIHPLG LYNHFPKWMM FASVLPALLV FILIFLESQI
710 720 730 740 750
TTLIVSKPER KMIKGSGFHL DLLLVVGMGG VAALFGMPWL SATTVRSVTH
760 770 780 790 800
ANALTVMGKA SGPGAAAQIQ EVKEQRISGL LVSVLVGLSI LMEPILSRIP
810 820 830 840 850
LAVLFGIFLY MGITSLSGIQ LFDRILLLFK PPKYHPDVPF VKRVKTWRMH
860 870 880 890 900
LFTGIQIICL AVLWVVKSTP ASLALPFVLI LTVPLRRLLL PLIFRELELQ
910 920
CLDGDDAKVT FDEAEGLDEY DEVPMPV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K8D8 | A0A0G2K8D8_RAT | Band 3 anion transport protein | Slc4a1 | 923 | Annotation score: | ||
F8WFT7 | F8WFT7_RAT | Anion exchange protein | Slc4a1 | 856 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000455 | 1 – 79 | Missing in isoform 2. CuratedAdd BLAST | 79 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04793 Genomic DNA Translation: AAA40800.1 Different initiation. L02943 mRNA Translation: AAA40801.1 |
PIRi | A33810 A48854 |
RefSeqi | NP_036783.2, NM_012651.2 XP_008766168.1, XM_008767946.2 [P23562-1] |
Genome annotation databases
GeneIDi | 24779 |
KEGGi | rno:24779 |
UCSCi | RGD:3710, rat [P23562-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04793 Genomic DNA Translation: AAA40800.1 Different initiation. L02943 mRNA Translation: AAA40801.1 |
PIRi | A33810 A48854 |
RefSeqi | NP_036783.2, NM_012651.2 XP_008766168.1, XM_008767946.2 [P23562-1] |
3D structure databases
AlphaFoldDBi | P23562 |
SMRi | P23562 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P23562, 1 interactor |
STRINGi | 10116.ENSRNOP00000028445 |
PTM databases
GlyGeni | P23562, 1 site |
iPTMneti | P23562 |
PhosphoSitePlusi | P23562 |
Proteomic databases
PaxDbi | P23562 |
PeptideAtlasi | P23562 |
Genome annotation databases
GeneIDi | 24779 |
KEGGi | rno:24779 |
UCSCi | RGD:3710, rat [P23562-1] |
Organism-specific databases
CTDi | 6521 |
RGDi | 3710, Slc4a1 |
Phylogenomic databases
eggNOGi | KOG1172, Eukaryota |
InParanoidi | P23562 |
OrthoDBi | 265068at2759 |
PhylomeDBi | P23562 |
Enzyme and pathway databases
Reactomei | R-RNO-1237044, Erythrocytes take up carbon dioxide and release oxygen R-RNO-1247673, Erythrocytes take up oxygen and release carbon dioxide R-RNO-425381, Bicarbonate transporters |
Miscellaneous databases
PROi | PR:P23562 |
Family and domain databases
Gene3Di | 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR001717, Anion_exchange IPR002977, Anion_exchange_1 IPR018241, Anion_exchange_CS IPR013769, Band3_cytoplasmic_dom IPR011531, HCO3_transpt-like_TM_dom IPR003020, HCO3_transpt_euk IPR016152, PTrfase/Anion_transptr |
PANTHERi | PTHR11453, PTHR11453, 1 hit |
Pfami | View protein in Pfam PF07565, Band_3_cyto, 1 hit PF00955, HCO3_cotransp, 2 hits |
PRINTSi | PR00165, ANIONEXCHNGR PR01187, ANIONEXHNGR1 PR01231, HCO3TRNSPORT |
SUPFAMi | SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00834, ae, 1 hit |
PROSITEi | View protein in PROSITE PS00219, ANION_EXCHANGER_1, 1 hit PS00220, ANION_EXCHANGER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | B3AT_RAT | |
Accessioni | P23562Primary (citable) accession number: P23562 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | March 27, 2002 | |
Last modified: | May 25, 2022 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families