UniProtKB - P23545 (PHOR_BACSU)
Protein
Alkaline phosphatase synthesis sensor protein PhoR
Gene
phoR
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Member of the two-component regulatory system PhoP/PhoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates PhoP in response to environmental signals.
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine. EC:2.7.13.3
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- histidine phosphotransfer kinase activity Source: GO_Central
- phosphorelay sensor kinase activity Source: GO_Central
GO - Biological processi
- phosphate ion transport Source: UniProtKB-KW
- protein autophosphorylation Source: GO_Central
- regulation of transcription, DNA-templated Source: InterPro
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphate transport, Transport, Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU29100-MONOMER |
BRENDAi | 2.7.13.3, 658 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:phoR Ordered Locus Names:BSU29100 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 8 – 28 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 29 – 150 | ExtracellularSequence analysisAdd BLAST | 122 | |
Transmembranei | 151 – 171 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 172 – 579 | CytoplasmicSequence analysisAdd BLAST | 408 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074845 | 1 – 579 | Alkaline phosphatase synthesis sensor protein PhoRAdd BLAST | 579 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 360 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P23545 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23545 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23545 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 234 – 300 | PASAdd BLAST | 67 | |
Domaini | 357 – 575 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 219 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG5002, Bacteria |
InParanoidi | P23545 |
OMAi | ASLCTAF |
PhylomeDBi | P23545 |
Family and domain databases
CDDi | cd00082, HisKA, 1 hit cd00130, PAS, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR031967, PhoR_single_Cache-like_dom IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit PF00989, PAS, 1 hit PF16736, sCache_like, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit SM00091, PAS, 1 hit |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55785, SSF55785, 1 hit SSF55874, SSF55874, 1 hit |
TIGRFAMsi | TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P23545-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKYRVRLFS VFVVCMILVF CVLGLFLQQL FETSDQRKAE EHIEKEAKYL
60 70 80 90 100
ASLLDAGNLN NQANEKIIKD AGGALDVSAS VIDTDGKVLY GSNGRSADSQ
110 120 130 140 150
KVQALVSGHE GILSTTDNKL YYGLSLRSEG EKTGYVLLSA SEKSDGLKGE
160 170 180 190 200
LWGMLTASLC TAFIVIVYFY SSMTSRYKRS IESATNVATE LSKGNYDART
210 220 230 240 250
YGGYIRRSDK LGHAMNSLAI DLMEMTRTQE MQRDRLLTVI ENIGSGLIMI
260 270 280 290 300
DGRGFINLVN RSYAKQFHIN PNHMLRRLYH DAFEHEEVIQ LVEDIFMTET
310 320 330 340 350
KKCKLLRLPI KIERRYFEVD GVPIMGPDDE WKGIVLVFHD MTETKKLEQM
360 370 380 390 400
RKDFVANVSH ELKTPITSIK GFTETLLDGA MEDKEALSEF LSIILKESER
410 420 430 440 450
LQSLVQDLLD LSKIEQQNFT LSIETFEPAK MLGEIETLLK HKADEKGISL
460 470 480 490 500
HLNVPKDPQY VSGDPYRLKQ VFLNLVNNAL TYTPEGGSVA INVKPREKDI
510 520 530 540 550
QIEVADSGIG IQKEEIPRIF ERFYRVDKDR SRNSGGTGLG LAIVKHLIEA
560 570
HEGKIDVTSE LGRGTVFTVT LKRAAEKSA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23549 Genomic DNA Translation: AAA22663.1 AF008220 Genomic DNA Translation: AAC00349.1 AL009126 Genomic DNA Translation: CAB14870.1 |
PIRi | A27650 |
RefSeqi | NP_390788.1, NC_000964.3 WP_004398493.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14870; CAB14870; BSU_29100 |
GeneIDi | 937387 |
KEGGi | bsu:BSU29100 |
PATRICi | fig|224308.179.peg.3159 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M23549 Genomic DNA Translation: AAA22663.1 AF008220 Genomic DNA Translation: AAC00349.1 AL009126 Genomic DNA Translation: CAB14870.1 |
PIRi | A27650 |
RefSeqi | NP_390788.1, NC_000964.3 WP_004398493.1, NZ_JNCM01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3CWF | X-ray | 2.20 | A/B | 32-150 | [»] | |
SMRi | P23545 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU29100 |
Proteomic databases
PaxDbi | P23545 |
Genome annotation databases
EnsemblBacteriai | CAB14870; CAB14870; BSU_29100 |
GeneIDi | 937387 |
KEGGi | bsu:BSU29100 |
PATRICi | fig|224308.179.peg.3159 |
Phylogenomic databases
eggNOGi | COG5002, Bacteria |
InParanoidi | P23545 |
OMAi | ASLCTAF |
PhylomeDBi | P23545 |
Enzyme and pathway databases
BioCyci | BSUB:BSU29100-MONOMER |
BRENDAi | 2.7.13.3, 658 |
Miscellaneous databases
EvolutionaryTracei | P23545 |
Family and domain databases
CDDi | cd00082, HisKA, 1 hit cd00130, PAS, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR031967, PhoR_single_Cache-like_dom IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit PF00989, PAS, 1 hit PF16736, sCache_like, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit SM00091, PAS, 1 hit |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55785, SSF55785, 1 hit SSF55874, SSF55874, 1 hit |
TIGRFAMsi | TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHOR_BACSU | |
Accessioni | P23545Primary (citable) accession number: P23545 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | April 7, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references