UniProtKB - P23542 (AATC_YEAST)
Protein
Aspartate aminotransferase, cytoplasmic
Gene
AAT2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Plays a key role in amino acid metabolism.
Miscellaneous
In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Present with 7700 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.6.1.13 Publications
Cofactori
pyridoxal 5'-phosphate2 Publications
Kineticsi
- KM=0.11 mM for L-aspartate1 Publication
- KM=20 mM for L-glutamate1 Publication
- KM=0.006 mM for oxaloacetate1 Publication
- KM=0.16 mM for 2-oxoglutarate1 Publication
pH dependencei
Optimum pH is 8-9.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 38 | Aspartate; via amide nitrogen1 Publication | 1 | |
Binding sitei | 135 | Aspartate1 Publication | 1 | |
Binding sitei | 188 | Aspartate1 Publication | 1 | |
Binding sitei | 387 | Aspartate1 Publication | 1 |
GO - Molecular functioni
- L-aspartate:2-oxoglutarate aminotransferase activity Source: SGD
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- asparagine biosynthetic process from oxaloacetate Source: SGD
- aspartate biosynthetic process Source: SGD
- aspartate metabolic process Source: UniProtKB
- glutamate metabolic process Source: UniProtKB
Keywordsi
Molecular function | Aminotransferase, Transferase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | MetaCyc:YLR027C-MONOMER |
Reactomei | R-SCE-70263, Gluconeogenesis R-SCE-8963693, Aspartate and asparagine metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate aminotransferase, cytoplasmic (EC:2.6.1.1)Alternative name(s): Transaminase A |
Gene namesi | Name:AAT2 Synonyms:ASP5 Ordered Locus Names:YLR027C ORF Names:L1746 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004017, AAT2 |
VEuPathDBi | FungiDB:YLR027C |
Subcellular locationi
Peroxisome
- Peroxisome 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Targeted to peroxisomes in cells grown in oleate.
Cytosol
- cytosol Source: SGD
Peroxisome
- peroxisome Source: SGD
Keywords - Cellular componenti
Cytoplasm, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources2 Publications | |||
ChainiPRO_0000123875 | 2 – 418 | Aspartate aminotransferase, cytoplasmicAdd BLAST | 417 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources1 Publication | 1 | |
Modified residuei | 255 | N6-(pyridoxal phosphate)lysine1 Publication | 1 | |
Modified residuei | 389 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P23542 |
PaxDbi | P23542 |
PRIDEi | P23542 |
PTM databases
iPTMneti | P23542 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 31301, 201 interactors |
DIPi | DIP-2897N |
IntActi | P23542, 18 interactors |
MINTi | P23542 |
STRINGi | 4932.YLR027C |
Miscellaneous databases
RNActi | P23542, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23542 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23542 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | KOG1412, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P23542 |
OMAi | IRSYHYW |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P23542-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSATLFNNIE LLPPDALFGI KQRYGQDQRA TKVDLGIGAY RDDNGKPWVL
60 70 80 90 100
PSVKAAEKLI HNDSSYNHEY LGITGLPSLT SNAAKIIFGT QSDAFQEDRV
110 120 130 140 150
ISVQSLSGTG ALHISAKFFS KFFPDKLVYL SKPTWANHMA IFENQGLKTA
160 170 180 190 200
TYPYWANETK SLDLNGFLNA IQKAPEGSIF VLHSCAHNPT GLDPTSEQWV
210 220 230 240 250
QIVDAIASKN HIALFDTAYQ GFATGDLDKD AYAVRLGVEK LSTVSPVFVC
260 270 280 290 300
QSFAKNAGMY GERVGCFHLA LTKQAQNKTI KPAVTSQLAK IIRSEVSNPP
310 320 330 340 350
AYGAKIVAKL LETPELTEQW HKDMVTMSSR ITKMRHALRD HLVKLGTPGN
360 370 380 390 400
WDHIVNQCGM FSFTGLTPQM VKRLEETHAV YLVASGRASI AGLNQGNVEY
410
VAKAIDEVVR FYTIEAKL
Sequence cautioni
The sequence CAA97550 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 95 | F → L AA sequence (PubMed:2199266).Curated | 1 | |
Sequence conflicti | 95 | F → L AA sequence (PubMed:1859361).Curated | 1 | |
Sequence conflicti | 413 – 414 | TI → AT AA sequence (PubMed:2199266).Curated | 2 | |
Sequence conflicti | 413 – 414 | TI → AT AA sequence (PubMed:1859361).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z73199 Genomic DNA Translation: CAA97550.1 Different initiation. BK006945 Genomic DNA Translation: DAA09345.1 |
PIRi | S64854 |
RefSeqi | NP_013127.2, NM_001181914.1 |
Genome annotation databases
EnsemblFungii | YLR027C_mRNA; YLR027C; YLR027C |
GeneIDi | 850714 |
KEGGi | sce:YLR027C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z73199 Genomic DNA Translation: CAA97550.1 Different initiation. BK006945 Genomic DNA Translation: DAA09345.1 |
PIRi | S64854 |
RefSeqi | NP_013127.2, NM_001181914.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YAA | X-ray | 2.05 | A/B/C/D | 2-412 | [»] | |
SMRi | P23542 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31301, 201 interactors |
DIPi | DIP-2897N |
IntActi | P23542, 18 interactors |
MINTi | P23542 |
STRINGi | 4932.YLR027C |
PTM databases
iPTMneti | P23542 |
Proteomic databases
MaxQBi | P23542 |
PaxDbi | P23542 |
PRIDEi | P23542 |
Genome annotation databases
EnsemblFungii | YLR027C_mRNA; YLR027C; YLR027C |
GeneIDi | 850714 |
KEGGi | sce:YLR027C |
Organism-specific databases
SGDi | S000004017, AAT2 |
VEuPathDBi | FungiDB:YLR027C |
Phylogenomic databases
eggNOGi | KOG1412, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P23542 |
OMAi | IRSYHYW |
Enzyme and pathway databases
BioCyci | MetaCyc:YLR027C-MONOMER |
Reactomei | R-SCE-70263, Gluconeogenesis R-SCE-8963693, Aspartate and asparagine metabolism |
Miscellaneous databases
EvolutionaryTracei | P23542 |
PROi | PR:P23542 |
RNActi | P23542, protein |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AATC_YEAST | |
Accessioni | P23542Primary (citable) accession number: P23542 Secondary accession number(s): D6VY29 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 200 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families