UniProtKB - P23528 (COF1_HUMAN)
Cofilin-1
CFL1
Functioni
GO - Molecular functioni
- actin filament binding Source: UniProtKB
GO - Biological processi
- actin cytoskeleton organization Source: ProtInc
- actin filament depolymerization Source: UniProtKB
- actin filament fragmentation Source: GO_Central
- actin filament severing Source: GO_Central
- cell motility Source: GO_Central
- cytoskeleton organization Source: UniProtKB
- establishment of spindle localization Source: UniProtKB
- interleukin-12-mediated signaling pathway Source: Reactome
- negative regulation of apoptotic process Source: UniProtKB
- positive regulation by host of viral process Source: AgBase
- positive regulation of embryonic development Source: UniProtKB
- regulation of cell morphogenesis Source: UniProtKB
- regulation of dendritic spine morphogenesis Source: ParkinsonsUK-UCL
- response to virus Source: UniProtKB
- Rho protein signal transduction Source: ProtInc
- viral process Source: UniProtKB-KW
Keywordsi
Molecular function | Actin-binding |
Biological process | Host-virus interaction |
Enzyme and pathway databases
PathwayCommonsi | P23528 |
Reactomei | R-HSA-114608, Platelet degranulation R-HSA-2029482, Regulation of actin dynamics for phagocytic cup formation R-HSA-3928662, EPHB-mediated forward signaling R-HSA-399954, Sema3A PAK dependent Axon repulsion R-HSA-5627117, RHO GTPases Activate ROCKs R-HSA-8950505, Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation |
SignaLinki | P23528 |
SIGNORi | P23528 |
Names & Taxonomyi
Protein namesi | Recommended name: Cofilin-1Alternative name(s): 18 kDa phosphoprotein Short name: p18 Cofilin, non-muscle isoform |
Gene namesi | Name:CFL1 Synonyms:CFL |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1874, CFL1 |
MIMi | 601442, gene |
neXtProti | NX_P23528 |
VEuPathDBi | HostDB:ENSG00000172757.12 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Nucleus
- Nucleus matrix 1 Publication
Plasma membrane
- ruffle membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
- lamellipodium membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Other locations
- lamellipodium By similarity
Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
Cytoskeleton
- actin cytoskeleton Source: GO_Central
- cortical actin cytoskeleton Source: GO_Central
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular space Source: UniProtKB
Nucleus
- nuclear matrix Source: UniProtKB-SubCell
- nucleus Source: UniProtKB
Plasma Membrane
- lamellipodium membrane Source: UniProtKB-SubCell
- ruffle membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: AgBase
- focal adhesion Source: MGI
- lamellipodium Source: UniProtKB
- membrane Source: UniProtKB
- vesicle Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 1072 |
OpenTargetsi | ENSG00000172757 |
PharmGKBi | PA26423 |
Miscellaneous databases
Pharosi | P23528, Tbio |
Chemistry databases
ChEMBLi | CHEMBL1075129 |
DrugBanki | DB11638, Artenimol DB09130, Copper DB04147, Dodecyldimethylamine N-oxide |
Genetic variation databases
BioMutai | CFL1 |
DMDMi | 116848 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources2 Publications | |||
ChainiPRO_0000214898 | 2 – 166 | Cofilin-1Add BLAST | 165 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources2 Publications | 1 | |
Modified residuei | 3 | Phosphoserine; by NRKCombined sources2 Publications | 1 | |
Modified residuei | 8 | PhosphoserineBy similarity | 1 | |
Modified residuei | 13 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 25 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 41 | PhosphoserineCombined sources | 1 | |
Modified residuei | 63 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 68 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 73 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 82 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 108 | Phosphoserine1 Publication | 1 | |
Cross-linki | 132 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 140 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 144 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 156 | PhosphoserineCombined sources1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P23528 |
jPOSTi | P23528 |
MassIVEi | P23528 |
PaxDbi | P23528 |
PeptideAtlasi | P23528 |
PRIDEi | P23528 |
ProteomicsDBi | 54128 |
TopDownProteomicsi | P23528 |
2D gel databases
DOSAC-COBS-2DPAGEi | P23528 |
OGPi | P23528 |
REPRODUCTION-2DPAGEi | IPI00012011 |
SWISS-2DPAGEi | P23528 |
UCD-2DPAGEi | P23528 |
PTM databases
CarbonylDBi | P23528 |
iPTMneti | P23528 |
MetOSitei | P23528 |
PhosphoSitePlusi | P23528 |
SwissPalmi | P23528 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000172757, Expressed in cerebellum and 256 other tissues |
ExpressionAtlasi | P23528, baseline and differential |
Genevisiblei | P23528, HS |
Organism-specific databases
HPAi | ENSG00000172757, Low tissue specificity |
Interactioni
Subunit structurei
Can bind G- and F-actin in a 1:1 ratio of cofilin to actin (PubMed:11812157). It is a major component of intranuclear and cytoplasmic actin rods (By similarity).
Interacts with the subcortical maternal complex (SCMC) via interaction with TLE6 isoform 1 and NLRP5 (By similarity).
By similarity1 Publication(Microbial infection) Interacts with human respiratory syncytial virus (HRSV) matrix protein; this interaction probably facilitates viral replication.
1 PublicationBinary interactionsi
Hide detailsP23528
GO - Molecular functioni
- actin filament binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 107499, 233 interactors |
CORUMi | P23528 |
DIPi | DIP-33000N |
IntActi | P23528, 113 interactors |
MINTi | P23528 |
STRINGi | 9606.ENSP00000432660 |
Miscellaneous databases
RNActi | P23528, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P23528 |
SMRi | P23528 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23528 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 153 | ADF-HPROSITE-ProRule annotationAdd BLAST | 150 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 30 – 34 | Nuclear localization signalSequence analysis | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1735, Eukaryota |
GeneTreei | ENSGT00950000183000 |
InParanoidi | P23528 |
OrthoDBi | 1370477at2759 |
PhylomeDBi | P23528 |
TreeFami | TF328601 |
Family and domain databases
CDDi | cd11286, ADF_cofilin_like, 1 hit |
Gene3Di | 3.40.20.10, 1 hit |
IDEALi | IID00627 |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR017904, ADF/Cofilin IPR027234, Cofilin_1 |
PANTHERi | PTHR11913, PTHR11913, 1 hit PTHR11913:SF17, PTHR11913:SF17, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 1 hit |
PRINTSi | PR00006, COFILIN |
SMARTi | View protein in SMART SM00102, ADF, 1 hit |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE
60 70 80 90 100
EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV
110 120 130 140 150
FIFWAPESAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA
160
EKLGGSAVIS LEGKPL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PK25 | E9PK25_HUMAN | Cofilin, non-muscle isoform | CFL1 | 204 | Annotation score: | ||
E9PQB7 | E9PQB7_HUMAN | Cofilin, non-muscle isoform | CFL1 | 122 | Annotation score: | ||
G3V1A4 | G3V1A4_HUMAN | Cofilin, non-muscle isoform | CFL1 hCG_2039457 | 149 | Annotation score: | ||
E9PP50 | E9PP50_HUMAN | Cofilin, non-muscle isoform | CFL1 | 159 | Annotation score: | ||
E9PS23 | E9PS23_HUMAN | Cofilin, non-muscle isoform | CFL1 | 90 | Annotation score: | ||
E9PLJ3 | E9PLJ3_HUMAN | Cofilin, non-muscle isoform | CFL1 | 79 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00682 mRNA Translation: BAA00589.1 U21909 mRNA Translation: AAA64501.1 X95404 mRNA Translation: CAA64685.1 BT006846 mRNA Translation: AAP35492.1 AK097690 mRNA Translation: BAG53513.1 CH471076 Genomic DNA Translation: EAW74449.1 BC011005 mRNA Translation: AAH11005.1 BC012265 mRNA Translation: AAH12265.1 BC012318 mRNA Translation: AAH12318.1 BC018256 mRNA Translation: AAH18256.1 |
CCDSi | CCDS8114.1 |
PIRi | S12632 |
RefSeqi | NP_005498.1, NM_005507.2 |
Genome annotation databases
Ensembli | ENST00000308162; ENSP00000309629; ENSG00000172757 ENST00000525451; ENSP00000432660; ENSG00000172757 |
GeneIDi | 1072 |
KEGGi | hsa:1072 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Cofilin entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00682 mRNA Translation: BAA00589.1 U21909 mRNA Translation: AAA64501.1 X95404 mRNA Translation: CAA64685.1 BT006846 mRNA Translation: AAP35492.1 AK097690 mRNA Translation: BAG53513.1 CH471076 Genomic DNA Translation: EAW74449.1 BC011005 mRNA Translation: AAH11005.1 BC012265 mRNA Translation: AAH12265.1 BC012318 mRNA Translation: AAH12318.1 BC018256 mRNA Translation: AAH18256.1 |
CCDSi | CCDS8114.1 |
PIRi | S12632 |
RefSeqi | NP_005498.1, NM_005507.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Q8G | NMR | - | A | 1-166 | [»] | |
1Q8X | NMR | - | A | 1-166 | [»] | |
3J0S | electron microscopy | 9.00 | M/N/O/P/Q/R/S/T/U/V/W/X | 1-166 | [»] | |
4BEX | X-ray | 2.80 | 1 | 1-166 | [»] | |
5HVK | X-ray | 3.50 | B/D | 2-166 | [»] | |
5L6W | X-ray | 2.53 | C | 1-166 | [»] | |
6UBY | electron microscopy | 7.50 | I | 1-166 | [»] | |
6UC0 | electron microscopy | 7.50 | I | 1-166 | [»] | |
6UC4 | electron microscopy | 9.20 | I/M/N/O/P | 1-166 | [»] | |
6VAO | electron microscopy | 3.40 | F/G/H/I/J | 1-166 | [»] | |
BMRBi | P23528 | |||||
SMRi | P23528 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107499, 233 interactors |
CORUMi | P23528 |
DIPi | DIP-33000N |
IntActi | P23528, 113 interactors |
MINTi | P23528 |
STRINGi | 9606.ENSP00000432660 |
Chemistry databases
ChEMBLi | CHEMBL1075129 |
DrugBanki | DB11638, Artenimol DB09130, Copper DB04147, Dodecyldimethylamine N-oxide |
PTM databases
CarbonylDBi | P23528 |
iPTMneti | P23528 |
MetOSitei | P23528 |
PhosphoSitePlusi | P23528 |
SwissPalmi | P23528 |
Genetic variation databases
BioMutai | CFL1 |
DMDMi | 116848 |
2D gel databases
DOSAC-COBS-2DPAGEi | P23528 |
OGPi | P23528 |
REPRODUCTION-2DPAGEi | IPI00012011 |
SWISS-2DPAGEi | P23528 |
UCD-2DPAGEi | P23528 |
Proteomic databases
EPDi | P23528 |
jPOSTi | P23528 |
MassIVEi | P23528 |
PaxDbi | P23528 |
PeptideAtlasi | P23528 |
PRIDEi | P23528 |
ProteomicsDBi | 54128 |
TopDownProteomicsi | P23528 |
Protocols and materials databases
Antibodypediai | 16010, 1014 antibodies |
DNASUi | 1072 |
Genome annotation databases
Ensembli | ENST00000308162; ENSP00000309629; ENSG00000172757 ENST00000525451; ENSP00000432660; ENSG00000172757 |
GeneIDi | 1072 |
KEGGi | hsa:1072 |
Organism-specific databases
CTDi | 1072 |
DisGeNETi | 1072 |
GeneCardsi | CFL1 |
HGNCi | HGNC:1874, CFL1 |
HPAi | ENSG00000172757, Low tissue specificity |
MIMi | 601442, gene |
neXtProti | NX_P23528 |
OpenTargetsi | ENSG00000172757 |
PharmGKBi | PA26423 |
VEuPathDBi | HostDB:ENSG00000172757.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1735, Eukaryota |
GeneTreei | ENSGT00950000183000 |
InParanoidi | P23528 |
OrthoDBi | 1370477at2759 |
PhylomeDBi | P23528 |
TreeFami | TF328601 |
Enzyme and pathway databases
PathwayCommonsi | P23528 |
Reactomei | R-HSA-114608, Platelet degranulation R-HSA-2029482, Regulation of actin dynamics for phagocytic cup formation R-HSA-3928662, EPHB-mediated forward signaling R-HSA-399954, Sema3A PAK dependent Axon repulsion R-HSA-5627117, RHO GTPases Activate ROCKs R-HSA-8950505, Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation |
SignaLinki | P23528 |
SIGNORi | P23528 |
Miscellaneous databases
BioGRID-ORCSi | 1072, 375 hits in 873 CRISPR screens |
ChiTaRSi | CFL1, human |
EvolutionaryTracei | P23528 |
GeneWikii | Cofilin_1 |
GenomeRNAii | 1072 |
Pharosi | P23528, Tbio |
PROi | PR:P23528 |
RNActi | P23528, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000172757, Expressed in cerebellum and 256 other tissues |
ExpressionAtlasi | P23528, baseline and differential |
Genevisiblei | P23528, HS |
Family and domain databases
CDDi | cd11286, ADF_cofilin_like, 1 hit |
Gene3Di | 3.40.20.10, 1 hit |
IDEALi | IID00627 |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR017904, ADF/Cofilin IPR027234, Cofilin_1 |
PANTHERi | PTHR11913, PTHR11913, 1 hit PTHR11913:SF17, PTHR11913:SF17, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 1 hit |
PRINTSi | PR00006, COFILIN |
SMARTi | View protein in SMART SM00102, ADF, 1 hit |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | COF1_HUMAN | |
Accessioni | P23528Primary (citable) accession number: P23528 Secondary accession number(s): B3KUQ1, Q53Y87, Q9UCA2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 232 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families