UniProtKB - P23511 (NFYA_HUMAN)
Nuclear transcription factor Y subunit alpha
NFYA
Functioni
Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 296 – 321 | NFYA/HAP2-typePROSITE-ProRule annotationAdd BLAST | 26 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription factor activity Source: ProtInc
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- histone H3-K4 trimethylation Source: ComplexPortal
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: ComplexPortal
- regulation of histone acetylation Source: ComplexPortal
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- rhythmic process Source: UniProtKB-KW
- transcription by RNA polymerase II Source: ProtInc
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | P23511 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-2426168, Activation of gene expression by SREBF (SREBP) R-HSA-380994, ATF4 activates genes in response to endoplasmic reticulum stress R-HSA-381183, ATF6 (ATF6-alpha) activates chaperone genes R-HSA-9614657, FOXO-mediated transcription of cell death genes |
SignaLinki | P23511 |
SIGNORi | P23511 |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear transcription factor Y subunit alphaAlternative name(s): CAAT box DNA-binding protein subunit A Nuclear transcription factor Y subunit A Short name: NF-YA |
Gene namesi | Name:NFYA |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7804, NFYA |
MIMi | 189903, gene |
neXtProti | NX_P23511 |
VEuPathDBi | HostDB:ENSG00000001167 |
Subcellular locationi
Nucleus
Nucleus
- CCAAT-binding factor complex Source: UniProtKB
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
- RNA polymerase II transcription regulator complex Source: NTNU_SB
Other locations
- chromatin Source: NTNU_SB
- protein-DNA complex Source: ParkinsonsUK-UCL
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 4800 |
OpenTargetsi | ENSG00000001167 |
PharmGKBi | PA31609 |
Miscellaneous databases
Pharosi | P23511, Tbio |
Genetic variation databases
BioMutai | NFYA |
DMDMi | 115844 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198768 | 1 – 347 | Nuclear transcription factor Y subunit alphaAdd BLAST | 347 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 326 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P23511 |
jPOSTi | P23511 |
MassIVEi | P23511 |
MaxQBi | P23511 |
PaxDbi | P23511 |
PeptideAtlasi | P23511 |
PRIDEi | P23511 |
ProteomicsDBi | 54123 [P23511-1] 54124 [P23511-2] |
PTM databases
GlyGeni | P23511, 4 sites, 2 O-linked glycans (4 sites) |
iPTMneti | P23511 |
PhosphoSitePlusi | P23511 |
Expressioni
Gene expression databases
Bgeei | ENSG00000001167, Expressed in cortical plate and 232 other tissues |
ExpressionAtlasi | P23511, baseline and differential |
Genevisiblei | P23511, HS |
Organism-specific databases
HPAi | ENSG00000001167, Low tissue specificity |
Interactioni
Subunit structurei
Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.
Interacts with SP1; the interaction is inhibited by glycosylation of SP1.
Interacts with ZHX1.
Interacts (via N-terminus) with ZHX2 (via homeobox domain).
Interacts with ZFX3.
6 PublicationsBinary interactionsi
P23511
Isoform Short [P23511-2]
Protein-protein interaction databases
BioGRIDi | 110866, 73 interactors |
ComplexPortali | CPX-1956, CCAAT-binding factor complex |
CORUMi | P23511 |
IntActi | P23511, 39 interactors |
MINTi | P23511 |
STRINGi | 9606.ENSP00000345702 |
Miscellaneous databases
RNActi | P23511, protein |
Structurei
Secondary structure
3D structure databases
SASBDBi | P23511 |
SMRi | P23511 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 299 – 347 | DisorderedSequence analysisAdd BLAST | 49 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 266 – 289 | Subunit association domain (SAD)Add BLAST | 24 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 312 – 332 | Basic and acidic residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1561, Eukaryota |
GeneTreei | ENSGT00390000015714 |
HOGENOMi | CLU_071609_1_0_1 |
InParanoidi | P23511 |
OMAi | GNMMNSG |
OrthoDBi | 1269806at2759 |
PhylomeDBi | P23511 |
TreeFami | TF323257 |
Family and domain databases
InterProi | View protein in InterPro IPR018362, CCAAT-binding_factor_CS IPR001289, NFYA |
PANTHERi | PTHR12632, PTHR12632, 1 hit |
Pfami | View protein in Pfam PF02045, CBFB_NFYA, 1 hit |
PRINTSi | PR00616, CCAATSUBUNTB |
SMARTi | View protein in SMART SM00521, CBF, 1 hit |
PROSITEi | View protein in PROSITE PS00686, NFYA_HAP2_1, 1 hit PS51152, NFYA_HAP2_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEQYTANSNS STEQIVVQAG QIQQQQQGGV TAVQLQTEAQ VASASGQQVQ
60 70 80 90 100
TLQVVQGQPL MVQVSGGQLI TSTGQPIMVQ AVPGGQGQTI MQVPVSGTQG
110 120 130 140 150
LQQIQLVPPG QIQIQGGQAV QVQGQQGQTQ QIIIQQPQTA VTAGQTQTQQ
160 170 180 190 200
QIAVQGQQVA QTAEGQTIVY QPVNADGTIL QQVTVPVSGM ITIPAASLAG
210 220 230 240 250
AQIVQTGANT NTTSSGQGTV TVTLPVAGNV VNSGGMVMMV PGAGSVPAIQ
260 270 280 290 300
RIPLPGAEML EEEPLYVNAK QYHRILKRRQ ARAKLEAEGK IPKERRKYLH
310 320 330 340
ESRHRHAMAR KRGEGGRFFS PKEKDSPHMQ DPNQADEEAM TQIIRVS
The sequence of this isoform differs from the canonical sequence as follows:
26-54: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 273 | H → N in AAH39244 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000849 | 26 – 54 | Missing in isoform Short. 1 PublicationAdd BLAST | 29 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59711 mRNA Translation: CAA42231.1 AL031778 Genomic DNA No translation available. BC039244 mRNA Translation: AAH39244.1 M59079 mRNA Translation: AAA35950.1 |
CCDSi | CCDS4849.1 [P23511-1] CCDS4850.1 [P23511-2] |
PIRi | S22816, A39123 |
RefSeqi | NP_002496.1, NM_002505.4 [P23511-1] NP_068351.1, NM_021705.3 [P23511-2] |
Genome annotation databases
Ensembli | ENST00000341376; ENSP00000345702; ENSG00000001167 ENST00000353205; ENSP00000229418; ENSG00000001167 [P23511-2] |
GeneIDi | 4800 |
KEGGi | hsa:4800 |
MANE-Selecti | ENST00000341376.11; ENSP00000345702.6; NM_002505.5; NP_002496.1 |
UCSCi | uc003opo.4, human [P23511-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59711 mRNA Translation: CAA42231.1 AL031778 Genomic DNA No translation available. BC039244 mRNA Translation: AAH39244.1 M59079 mRNA Translation: AAA35950.1 |
CCDSi | CCDS4849.1 [P23511-1] CCDS4850.1 [P23511-2] |
PIRi | S22816, A39123 |
RefSeqi | NP_002496.1, NM_002505.4 [P23511-1] NP_068351.1, NM_021705.3 [P23511-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4AWL | X-ray | 3.08 | A | 262-332 | [»] | |
6QMP | X-ray | 2.00 | A | 267-295 | [»] | |
6QMQ | X-ray | 2.50 | A | 267-285 | [»] | |
6QMS | X-ray | 1.80 | A | 267-285 | [»] | |
SASBDBi | P23511 | |||||
SMRi | P23511 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110866, 73 interactors |
ComplexPortali | CPX-1956, CCAAT-binding factor complex |
CORUMi | P23511 |
IntActi | P23511, 39 interactors |
MINTi | P23511 |
STRINGi | 9606.ENSP00000345702 |
PTM databases
GlyGeni | P23511, 4 sites, 2 O-linked glycans (4 sites) |
iPTMneti | P23511 |
PhosphoSitePlusi | P23511 |
Genetic variation databases
BioMutai | NFYA |
DMDMi | 115844 |
Proteomic databases
EPDi | P23511 |
jPOSTi | P23511 |
MassIVEi | P23511 |
MaxQBi | P23511 |
PaxDbi | P23511 |
PeptideAtlasi | P23511 |
PRIDEi | P23511 |
ProteomicsDBi | 54123 [P23511-1] 54124 [P23511-2] |
Protocols and materials databases
Antibodypediai | 3982, 221 antibodies from 35 providers |
DNASUi | 4800 |
Genome annotation databases
Ensembli | ENST00000341376; ENSP00000345702; ENSG00000001167 ENST00000353205; ENSP00000229418; ENSG00000001167 [P23511-2] |
GeneIDi | 4800 |
KEGGi | hsa:4800 |
MANE-Selecti | ENST00000341376.11; ENSP00000345702.6; NM_002505.5; NP_002496.1 |
UCSCi | uc003opo.4, human [P23511-1] |
Organism-specific databases
CTDi | 4800 |
DisGeNETi | 4800 |
GeneCardsi | NFYA |
HGNCi | HGNC:7804, NFYA |
HPAi | ENSG00000001167, Low tissue specificity |
MIMi | 189903, gene |
neXtProti | NX_P23511 |
OpenTargetsi | ENSG00000001167 |
PharmGKBi | PA31609 |
VEuPathDBi | HostDB:ENSG00000001167 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1561, Eukaryota |
GeneTreei | ENSGT00390000015714 |
HOGENOMi | CLU_071609_1_0_1 |
InParanoidi | P23511 |
OMAi | GNMMNSG |
OrthoDBi | 1269806at2759 |
PhylomeDBi | P23511 |
TreeFami | TF323257 |
Enzyme and pathway databases
PathwayCommonsi | P23511 |
Reactomei | R-HSA-1989781, PPARA activates gene expression R-HSA-2426168, Activation of gene expression by SREBF (SREBP) R-HSA-380994, ATF4 activates genes in response to endoplasmic reticulum stress R-HSA-381183, ATF6 (ATF6-alpha) activates chaperone genes R-HSA-9614657, FOXO-mediated transcription of cell death genes |
SignaLinki | P23511 |
SIGNORi | P23511 |
Miscellaneous databases
BioGRID-ORCSi | 4800, 173 hits in 1067 CRISPR screens |
ChiTaRSi | NFYA, human |
GeneWikii | NFYA |
GenomeRNAii | 4800 |
Pharosi | P23511, Tbio |
PROi | PR:P23511 |
RNActi | P23511, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000001167, Expressed in cortical plate and 232 other tissues |
ExpressionAtlasi | P23511, baseline and differential |
Genevisiblei | P23511, HS |
Family and domain databases
InterProi | View protein in InterPro IPR018362, CCAAT-binding_factor_CS IPR001289, NFYA |
PANTHERi | PTHR12632, PTHR12632, 1 hit |
Pfami | View protein in Pfam PF02045, CBFB_NFYA, 1 hit |
PRINTSi | PR00616, CCAATSUBUNTB |
SMARTi | View protein in SMART SM00521, CBF, 1 hit |
PROSITEi | View protein in PROSITE PS00686, NFYA_HAP2_1, 1 hit PS51152, NFYA_HAP2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NFYA_HUMAN | |
Accessioni | P23511Primary (citable) accession number: P23511 Secondary accession number(s): Q8IXU0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | May 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families