Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytosolic Fe-S cluster assembly factor NAR1

Gene

NAR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of a cytosolic Fe/S protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. May play a role in the transfer of pre-assembled Fe/S clusters to target apoproteins.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi20Iron-sulfur 1 (4Fe-4S)Curated1
Metal bindingi59Iron-sulfur 1 (4Fe-4S)Curated1
Metal bindingi62Iron-sulfur 1 (4Fe-4S)Curated1
Metal bindingi65Iron-sulfur 1 (4Fe-4S)Curated1
Metal bindingi177Iron-sulfur 2 (4Fe-4S)Curated1
Metal bindingi231Iron-sulfur 2 (4Fe-4S)Curated1
Metal bindingi412Iron-sulfur 2 (4Fe-4S)Curated1
Metal bindingi416Iron-sulfur 2 (4Fe-4S)Curated1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • ferredoxin hydrogenase activity Source: SGD
  • iron-sulfur cluster binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • NADH dehydrogenase activity Source: GO_Central

GO - Biological processi

  • iron-sulfur cluster assembly Source: UniProtKB

Keywordsi

Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33238-MONOMER
ReactomeiR-SCE-2564818 Cytosolic iron-sulfur cluster assembly (yeast)

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NAR1
Alternative name(s):
Nuclear architecture-related protein 1
Gene namesi
Name:NAR1
Ordered Locus Names:YNL240C
ORF Names:N1114
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL240C
SGDiS000005184 NAR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi20C → A: Weakly reduced iron content and impaired maturation of cytosolic Fe/S proteins. Strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-62 or A-65. 1 Publication1
Mutagenesisi59C → A: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins. 1 Publication1
Mutagenesisi62C → A: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins. 1 Publication1
Mutagenesisi65C → A: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins. 1 Publication1
Mutagenesisi177C → A: Impaired maturation of cytosolic Fe/S proteins. Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-416. 1 Publication1
Mutagenesisi177C → S: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with S-412. 1 Publication1
Mutagenesisi231C → A: Impaired maturation of cytosolic Fe/S proteins. Strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-416. 1 Publication1
Mutagenesisi412C → S: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with S-177. 1 Publication1
Mutagenesisi416C → A: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-177 or A-231. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967181 – 491Cytosolic Fe-S cluster assembly factor NAR1Add BLAST491

Proteomic databases

MaxQBiP23503
PaxDbiP23503
PRIDEiP23503

PTM databases

iPTMnetiP23503

Interactioni

Subunit structurei

Interacts with CIA1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CIA1Q055832EBI-11864,EBI-32145

Protein-protein interaction databases

BioGridi35599, 303 interactors
IntActiP23503, 5 interactors
STRINGi4932.YNL240C

Structurei

3D structure databases

ProteinModelPortaliP23503
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NARF family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000017231
HOGENOMiHOG000191744
InParanoidiP23503
OMAiCIKPVET
OrthoDBiEOG092C2MTS

Family and domain databases

InterProiView protein in InterPro
IPR009016 Fe_hydrogenase
IPR004108 Fe_hydrogenase_lsu_C
PfamiView protein in Pfam
PF02906 Fe_hyd_lg_C, 1 hit
SUPFAMiSSF53920 SSF53920, 2 hits

Sequencei

Sequence statusi: Complete.

P23503-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSALLSESDL NDFISPALAC VKPTQVSGGK KDNVNMNGEY EVSTEPDQLE
60 70 80 90 100
KVSITLSDCL ACSGCITSSE EILLSSQSHS VFLKNWGKLS QQQDKFLVVS
110 120 130 140 150
VSPQCRLSLA QYYGLTLEAA DLCLMNFFQK HFQCKYMVGT EMGRIISISK
160 170 180 190 200
TVEKIIAHKK QKENTGADRK PLLSAVCPGF LIYTEKTKPQ LVPMLLNVKS
210 220 230 240 250
PQQITGSLIR ATFESLAIAR ESFYHLSLMP CFDKKLEASR PESLDDGIDC
260 270 280 290 300
VITPREIVTM LQELNLDFKS FLTEDTSLYG RLSPPGWDPR VHWASNLGGT
310 320 330 340 350
CGGYAYQYVT AVQRLHPGSQ MIVLEGRNSD IVEYRLLHDD RIIAAASELS
360 370 380 390 400
GFRNIQNLVR KLTSGSGSER KRNITALRKR RTGPKANSRE MAAATAATAD
410 420 430 440 450
PYHSDYIEVN ACPGACMNGG GLLNGEQNSL KRKQLVQTLN KRHGEELAMV
460 470 480 490
DPLTLGPKLE EAAARPLSLE YVFAPVKQAV EKDLVSVGST W
Length:491
Mass (Da):54,151
Last modified:October 1, 1996 - v2
Checksum:iFFEB2A07A881BBC1
GO

Sequence cautioni

The sequence AAA34618 differs from that shown. Reason: Frameshift at several positions.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69381 Genomic DNA Translation: CAA93358.1
Z71516 Genomic DNA Translation: CAA96145.1
AY692992 Genomic DNA Translation: AAT93011.1
M34709 Genomic DNA Translation: AAA34618.1 Frameshift.
BK006947 Genomic DNA Translation: DAA10319.1
PIRiS63206
RefSeqiNP_014159.1, NM_001183078.1

Genome annotation databases

EnsemblFungiiYNL240C; YNL240C; YNL240C
GeneIDi855481
KEGGisce:YNL240C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69381 Genomic DNA Translation: CAA93358.1
Z71516 Genomic DNA Translation: CAA96145.1
AY692992 Genomic DNA Translation: AAT93011.1
M34709 Genomic DNA Translation: AAA34618.1 Frameshift.
BK006947 Genomic DNA Translation: DAA10319.1
PIRiS63206
RefSeqiNP_014159.1, NM_001183078.1

3D structure databases

ProteinModelPortaliP23503
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35599, 303 interactors
IntActiP23503, 5 interactors
STRINGi4932.YNL240C

PTM databases

iPTMnetiP23503

Proteomic databases

MaxQBiP23503
PaxDbiP23503
PRIDEiP23503

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL240C; YNL240C; YNL240C
GeneIDi855481
KEGGisce:YNL240C

Organism-specific databases

EuPathDBiFungiDB:YNL240C
SGDiS000005184 NAR1

Phylogenomic databases

GeneTreeiENSGT00390000017231
HOGENOMiHOG000191744
InParanoidiP23503
OMAiCIKPVET
OrthoDBiEOG092C2MTS

Enzyme and pathway databases

BioCyciYEAST:G3O-33238-MONOMER
ReactomeiR-SCE-2564818 Cytosolic iron-sulfur cluster assembly (yeast)

Miscellaneous databases

PROiPR:P23503

Family and domain databases

InterProiView protein in InterPro
IPR009016 Fe_hydrogenase
IPR004108 Fe_hydrogenase_lsu_C
PfamiView protein in Pfam
PF02906 Fe_hyd_lg_C, 1 hit
SUPFAMiSSF53920 SSF53920, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNAR1_YEAST
AccessioniPrimary (citable) accession number: P23503
Secondary accession number(s): D6W0V3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again