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UniProtKB - P23503 (NAR1_YEAST)
Protein
Cytosolic Fe-S cluster assembly factor NAR1
Gene
NAR1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Essential component of a cytosolic Fe/S protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. May play a role in the transfer of pre-assembled Fe/S clusters to target apoproteins.
3 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 20 | Iron-sulfur (4Fe-4S) 1Curated | 1 | |
Metal bindingi | 59 | Iron-sulfur (4Fe-4S) 1Curated | 1 | |
Metal bindingi | 62 | Iron-sulfur (4Fe-4S) 1Curated | 1 | |
Metal bindingi | 65 | Iron-sulfur (4Fe-4S) 1Curated | 1 | |
Metal bindingi | 177 | Iron-sulfur (4Fe-4S) 2Curated | 1 | |
Metal bindingi | 231 | Iron-sulfur (4Fe-4S) 2Curated | 1 | |
Metal bindingi | 412 | Iron-sulfur (4Fe-4S) 2Curated | 1 | |
Metal bindingi | 416 | Iron-sulfur (4Fe-4S) 2Curated | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: GO_Central
- ferredoxin hydrogenase activity Source: SGD
- iron-sulfur cluster binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- iron-sulfur cluster assembly Source: UniProtKB
Keywordsi
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic Fe-S cluster assembly factor NAR1Alternative name(s): Nuclear architecture-related protein 1 |
Gene namesi | Name:NAR1 Ordered Locus Names:YNL240C ORF Names:N1114 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005184, NAR1 |
VEuPathDBi | FungiDB:YNL240C |
Subcellular locationi
Cytosol
- CIA complex Source: GO_Central
- cytosol Source: SGD
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- membrane Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 20 | C → A: Weakly reduced iron content and impaired maturation of cytosolic Fe/S proteins. Strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-62 or A-65. 1 Publication | 1 | |
Mutagenesisi | 59 | C → A: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins. 1 Publication | 1 | |
Mutagenesisi | 62 | C → A: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins. 1 Publication | 1 | |
Mutagenesisi | 65 | C → A: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins. 1 Publication | 1 | |
Mutagenesisi | 177 | C → A: Impaired maturation of cytosolic Fe/S proteins. Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-416. 1 Publication | 1 | |
Mutagenesisi | 177 | C → S: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with S-412. 1 Publication | 1 | |
Mutagenesisi | 231 | C → A: Impaired maturation of cytosolic Fe/S proteins. Strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-416. 1 Publication | 1 | |
Mutagenesisi | 412 | C → S: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with S-177. 1 Publication | 1 | |
Mutagenesisi | 416 | C → A: Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-177 or A-231. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096718 | 1 – 491 | Cytosolic Fe-S cluster assembly factor NAR1Add BLAST | 491 |
Proteomic databases
MaxQBi | P23503 |
PaxDbi | P23503 |
PRIDEi | P23503 |
PTM databases
iPTMneti | P23503 |
Interactioni
Subunit structurei
Interacts with CIA1.
1 PublicationBinary interactionsi
P23503
With | #Exp. | IntAct |
---|---|---|
CIA1 [Q05583] | 2 | EBI-11864,EBI-32145 |
Protein-protein interaction databases
BioGRIDi | 35599, 305 interactors |
IntActi | P23503, 5 interactors |
STRINGi | 4932.YNL240C |
Miscellaneous databases
RNActi | P23503, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the NARF family.Curated
Phylogenomic databases
eggNOGi | KOG2439, Eukaryota |
GeneTreei | ENSGT00940000153514 |
HOGENOMi | CLU_018240_0_1_1 |
InParanoidi | P23503 |
OMAi | FTWVVDT |
Family and domain databases
InterProi | View protein in InterPro IPR009016, Fe_hydrogenase IPR004108, Fe_hydrogenase_lsu_C |
Pfami | View protein in Pfam PF02906, Fe_hyd_lg_C, 1 hit |
SUPFAMi | SSF53920, SSF53920, 1 hit |
i Sequence
Sequence statusi: Complete.
P23503-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSALLSESDL NDFISPALAC VKPTQVSGGK KDNVNMNGEY EVSTEPDQLE
60 70 80 90 100
KVSITLSDCL ACSGCITSSE EILLSSQSHS VFLKNWGKLS QQQDKFLVVS
110 120 130 140 150
VSPQCRLSLA QYYGLTLEAA DLCLMNFFQK HFQCKYMVGT EMGRIISISK
160 170 180 190 200
TVEKIIAHKK QKENTGADRK PLLSAVCPGF LIYTEKTKPQ LVPMLLNVKS
210 220 230 240 250
PQQITGSLIR ATFESLAIAR ESFYHLSLMP CFDKKLEASR PESLDDGIDC
260 270 280 290 300
VITPREIVTM LQELNLDFKS FLTEDTSLYG RLSPPGWDPR VHWASNLGGT
310 320 330 340 350
CGGYAYQYVT AVQRLHPGSQ MIVLEGRNSD IVEYRLLHDD RIIAAASELS
360 370 380 390 400
GFRNIQNLVR KLTSGSGSER KRNITALRKR RTGPKANSRE MAAATAATAD
410 420 430 440 450
PYHSDYIEVN ACPGACMNGG GLLNGEQNSL KRKQLVQTLN KRHGEELAMV
460 470 480 490
DPLTLGPKLE EAAARPLSLE YVFAPVKQAV EKDLVSVGST W
Sequence cautioni
The sequence AAA34618 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z69381 Genomic DNA Translation: CAA93358.1 Z71516 Genomic DNA Translation: CAA96145.1 AY692992 Genomic DNA Translation: AAT93011.1 M34709 Genomic DNA Translation: AAA34618.1 Frameshift. BK006947 Genomic DNA Translation: DAA10319.1 |
PIRi | S63206 |
RefSeqi | NP_014159.1, NM_001183078.1 |
Genome annotation databases
EnsemblFungii | YNL240C_mRNA; YNL240C; YNL240C |
GeneIDi | 855481 |
KEGGi | sce:YNL240C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z69381 Genomic DNA Translation: CAA93358.1 Z71516 Genomic DNA Translation: CAA96145.1 AY692992 Genomic DNA Translation: AAT93011.1 M34709 Genomic DNA Translation: AAA34618.1 Frameshift. BK006947 Genomic DNA Translation: DAA10319.1 |
PIRi | S63206 |
RefSeqi | NP_014159.1, NM_001183078.1 |
3D structure databases
SMRi | P23503 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35599, 305 interactors |
IntActi | P23503, 5 interactors |
STRINGi | 4932.YNL240C |
PTM databases
iPTMneti | P23503 |
Proteomic databases
MaxQBi | P23503 |
PaxDbi | P23503 |
PRIDEi | P23503 |
Genome annotation databases
EnsemblFungii | YNL240C_mRNA; YNL240C; YNL240C |
GeneIDi | 855481 |
KEGGi | sce:YNL240C |
Organism-specific databases
SGDi | S000005184, NAR1 |
VEuPathDBi | FungiDB:YNL240C |
Phylogenomic databases
eggNOGi | KOG2439, Eukaryota |
GeneTreei | ENSGT00940000153514 |
HOGENOMi | CLU_018240_0_1_1 |
InParanoidi | P23503 |
OMAi | FTWVVDT |
Miscellaneous databases
PROi | PR:P23503 |
RNActi | P23503, protein |
Family and domain databases
InterProi | View protein in InterPro IPR009016, Fe_hydrogenase IPR004108, Fe_hydrogenase_lsu_C |
Pfami | View protein in Pfam PF02906, Fe_hyd_lg_C, 1 hit |
SUPFAMi | SSF53920, SSF53920, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NAR1_YEAST | |
Accessioni | P23503Primary (citable) accession number: P23503 Secondary accession number(s): D6W0V3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - SIMILARITY comments
Index of protein domains and families