UniProtKB - P23478 (ADDA_BACSU)
Protein
ATP-dependent helicase/nuclease subunit A
Gene
addA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
An essential component of the DNA double-stranded break repair machinery, the heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' -> 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddA nuclease domain in particular is required for chi fragment generation; this subunit has 3' -> 5' nuclease and helicase activity. RecA thread formation during DNA double-strand break repair requires RecJ or AddAB.3 Publications
Miscellaneous
This enzyme is a functional homolog of the E.coli RecBCD enzyme; unlike the RecBCD enzyme it degrades both duplex strands symmetrically.
Catalytic activityi
- EC:3.6.4.12
Cofactori
Mg2+1 PublicationNote: At low magnesium concentrations there is no nuclease activity, but helicase activity is unaffected.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 30 – 37 | ATPSequence analysis | 8 |
GO - Molecular functioni
- 3'-5' DNA helicase activity Source: GO_Central
- 3'-5' exonuclease activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-UniRule
- double-stranded DNA binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA repair Source: GO_Central
- double-strand break repair via homologous recombination Source: UniProtKB-UniRule
- recombinational repair Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Exonuclease, Helicase, Hydrolase, Nuclease |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU10630-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:addA Ordered Locus Names:BSU10630 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: GO_Central
- DNA helicase complex Source: GO_Central
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 36 | K → A: Loss of helicase and nuclease activity. DNA-binding is unaltered. 1 Publication | 1 | |
Mutagenesisi | 1172 | D → A: Some loss of nuclease activity, helicase and DNA-binding are unaltered. No production of chi fragments; when associated with A-961 in AddB nearly complete loss of nuclease activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064449 | 1 – 1232 | ATP-dependent helicase/nuclease subunit AAdd BLAST | 1232 |
Proteomic databases
PaxDbi | P23478 |
PRIDEi | P23478 |
Interactioni
Subunit structurei
Heterodimer of AddA and AddB.
2 PublicationsBinary interactionsi
P23478
With | #Exp. | IntAct |
---|---|---|
addB [P23477] | 4 | EBI-16098568,EBI-5247995 |
Protein-protein interaction databases
DIPi | DIP-60825N |
IntActi | P23478, 1 interactor |
STRINGi | 224308.BSU10630 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23478 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 481 | UvrD-like helicase ATP-bindingAdd BLAST | 473 | |
Domaini | 508 – 798 | UvrD-like helicase C-terminalAdd BLAST | 291 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1074, Bacteria |
InParanoidi | P23478 |
OMAi | KQSIYRW |
PhylomeDBi | P23478 |
Family and domain databases
Gene3Di | 3.90.320.10, 1 hit |
HAMAPi | MF_01451, AddA, 1 hit |
InterProi | View protein in InterPro IPR014152, DNA_helicase_suAddA IPR014017, DNA_helicase_UvrD-like_C IPR000212, DNA_helicase_UvrD/REP IPR011604, Exonuc_phg/RecB_C IPR027417, P-loop_NTPase IPR038726, PDDEXK_AddAB-type IPR011335, Restrct_endonuc-II-like IPR014016, UvrD-like_ATP-bd IPR034739, UvrD/AddA_N |
PANTHERi | PTHR11070, PTHR11070, 1 hit PTHR11070:SF48, PTHR11070:SF48, 1 hit |
Pfami | View protein in Pfam PF12705, PDDEXK_1, 1 hit PF00580, UvrD-helicase, 1 hit PF13361, UvrD_C, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF52980, SSF52980, 1 hit |
TIGRFAMsi | TIGR02785, addA_Gpos, 1 hit |
PROSITEi | View protein in PROSITE PS51198, UVRD_HELICASE_ATP_BIND, 1 hit PS51217, UVRD_HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P23478-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNIPKPADST WTDDQWNAIV STGQDILVAA AAGSGKTAVL VERMIRKITA
60 70 80 90 100
EENPIDVDRL LVVTFTNASA AEMKHRIAEA LEKELVQRPG SLHIRRQLSL
110 120 130 140 150
LNRASISTLH SFCLQVLKKY YYLIDLDPGF RIADQTEGEL IGDEVLDELF
160 170 180 190 200
EDEYAKGEKA FFELVDRYTT DRHDLDLQFL VKQVYEYSRS HPNPEAWLES
210 220 230 240 250
FVHLYDVSEK SAIEELPFYQ YVKEDIAMVL NGAKEKLLRA LELTKAPGGP
260 270 280 290 300
APRADNFLDD LAQIDELIQH QDDFSELYKR VPAVSFKRAK AVKGDEFDPA
310 320 330 340 350
LLDEATDLRN GAKKLLEKLK TDYFTRSPEQ HLKSLAEMKP VIETLVQLVI
360 370 380 390 400
SYGKRFEAAK QEKSIIDFSD LEHYCLAILT AENDKGEREP SEAARFYQEQ
410 420 430 440 450
FHEVLVDEYQ DTNLVQESIL QLVTSGPEET GNLFMVGDVK QSIYRFRLAE
460 470 480 490 500
PLLFLSKYKR FTESGEGTGR KIDLNKNFRS RADILDSTNF LFKQLMGGKI
510 520 530 540 550
GEVDYDEQAE LKLGAAYPDN DETETELLLI DNAEDTDASE EAEELETVQF
560 570 580 590 600
EAKAIAKEIR KLISSPFKVY DGKKKTHRNI QYRDIVILLR SMPWAPQIME
610 620 630 640 650
ELRAQGIPVY ANLTSGYFEA VEVAVALSVL KVIDNPYQDI PLASVLRSPI
660 670 680 690 700
VGADENELSL IRLENKKAPY YEAMKDYLAA GDRSDELYQK LNTFYGHLQK
710 720 730 740 750
WRAFSKNHSV SELIWEVYRD TKYMDYVGGM PGGKQRQANL RVLYDRARQY
760 770 780 790 800
ESTAFRGLFR FLRFIERMQE RGDDLGTARA LSEQEDVVRL MTIHSSKGLE
810 820 830 840 850
FPVVFVAGLG RNFNMMDLNK SYLLDKELGF GTKYIHPQLR ISYPTLPLIA
860 870 880 890 900
MKKKMRRELL SEELRVLYVA LTRAKEKLFL IGSCKDHQKQ LAKWQASASQ
910 920 930 940 950
TDWLLPEFDR YQARTYLDFI GPALARHRDL GDLAGVPAHA DISGHPARFA
960 970 980 990 1000
VQMIHSYDLL DDDLEERMEE KSERLEAIRR GEPVPGSFAF DEKAREQLSW
1010 1020 1030 1040 1050
TYPHQEVTQI RTKQSVSEIK RKREYEDEYS GRAPVKPADG SILYRRPAFM
1060 1070 1080 1090 1100
MKKGLTAAEK GTAMHTVMQH IPLSHVPSIE EAEQTVHRLY EKELLTEEQK
1110 1120 1130 1140 1150
DAIDIEEIVQ FFHTEIGGQL IGAKWKDREI PFSLALPAKE IYPDAHEADE
1160 1170 1180 1190 1200
PLLVQGIIDC LYETEDGLYL LDYKSDRIEG KFQHGFEGAA PILKKRYETQ
1210 1220 1230
IQLYTKAVEQ IAKTKVKGCA LYFFDGGHIL TL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 780 | A → G in AAA22201 (PubMed:1646786).Curated | 1 | |
Sequence conflicti | 780 | A → G in CAA74482 (PubMed:9579061).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63489 Genomic DNA Translation: AAA22201.1 Y14081 Genomic DNA Translation: CAA74482.1 AL009126 Genomic DNA Translation: CAB12903.2 Y09476 Genomic DNA Translation: CAA70668.1 |
PIRi | B39432 |
RefSeqi | NP_388944.2, NC_000964.3 WP_003233100.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB12903; CAB12903; BSU10630 |
GeneIDi | 939793 |
KEGGi | bsu:BSU10630 |
PATRICi | fig|224308.179.peg.1143 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63489 Genomic DNA Translation: AAA22201.1 Y14081 Genomic DNA Translation: CAA74482.1 AL009126 Genomic DNA Translation: CAB12903.2 Y09476 Genomic DNA Translation: CAA70668.1 |
PIRi | B39432 |
RefSeqi | NP_388944.2, NC_000964.3 WP_003233100.1, NZ_JNCM01000035.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3U44 | X-ray | 3.20 | A | 1-1232 | [»] | |
3U4Q | X-ray | 2.80 | A | 1-1232 | [»] | |
4CEH | X-ray | 3.24 | A | 1-1232 | [»] | |
4CEI | X-ray | 2.80 | A | 1-1232 | [»] | |
4CEJ | X-ray | 3.00 | A | 1-1232 | [»] | |
SMRi | P23478 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-60825N |
IntActi | P23478, 1 interactor |
STRINGi | 224308.BSU10630 |
Proteomic databases
PaxDbi | P23478 |
PRIDEi | P23478 |
Genome annotation databases
EnsemblBacteriai | CAB12903; CAB12903; BSU10630 |
GeneIDi | 939793 |
KEGGi | bsu:BSU10630 |
PATRICi | fig|224308.179.peg.1143 |
Phylogenomic databases
eggNOGi | COG1074, Bacteria |
InParanoidi | P23478 |
OMAi | KQSIYRW |
PhylomeDBi | P23478 |
Enzyme and pathway databases
BioCyci | BSUB:BSU10630-MONOMER |
Family and domain databases
Gene3Di | 3.90.320.10, 1 hit |
HAMAPi | MF_01451, AddA, 1 hit |
InterProi | View protein in InterPro IPR014152, DNA_helicase_suAddA IPR014017, DNA_helicase_UvrD-like_C IPR000212, DNA_helicase_UvrD/REP IPR011604, Exonuc_phg/RecB_C IPR027417, P-loop_NTPase IPR038726, PDDEXK_AddAB-type IPR011335, Restrct_endonuc-II-like IPR014016, UvrD-like_ATP-bd IPR034739, UvrD/AddA_N |
PANTHERi | PTHR11070, PTHR11070, 1 hit PTHR11070:SF48, PTHR11070:SF48, 1 hit |
Pfami | View protein in Pfam PF12705, PDDEXK_1, 1 hit PF00580, UvrD-helicase, 1 hit PF13361, UvrD_C, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF52980, SSF52980, 1 hit |
TIGRFAMsi | TIGR02785, addA_Gpos, 1 hit |
PROSITEi | View protein in PROSITE PS51198, UVRD_HELICASE_ATP_BIND, 1 hit PS51217, UVRD_HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADDA_BACSU | |
Accessioni | P23478Primary (citable) accession number: P23478 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | June 16, 2009 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families