UniProtKB - P23457 (DIDH_RAT)
Protein
3-alpha-hydroxysteroid dehydrogenase
Gene
Akr1c9
Organism
Rattus norvegicus (Rat)
Status
Functioni
Besides being a 3-alpha-hydroxysteroid dehydrogenase, the enzyme can accomplish diverse functions: as quinone reductase, as an aromatic alcohol dehydrogenase, as dihydrodiol dehydrogenase, and as 9-, 11-, and 15-hydroxyprostaglandin dehydrogenase.
Catalytic activityi
Activity regulationi
Potently inhibited by the nonsteroidal anti-inflammatory drugs (NSAID).
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 50 | NADP1 Publication | 1 | |
Active sitei | 55 | Proton donor | 1 | |
Sitei | 84 | Lowers pKa of active site TyrBy similarity | 1 | |
Binding sitei | 117 | Substrate | 1 | |
Binding sitei | 190 | NADP1 Publication | 1 | |
Binding sitei | 227 | Substrate | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 20 – 24 | NADP1 Publication | 5 | |
Nucleotide bindingi | 166 – 167 | NADP1 Publication | 2 | |
Nucleotide bindingi | 216 – 221 | NADP1 Publication | 6 | |
Nucleotide bindingi | 270 – 280 | NADP1 PublicationAdd BLAST | 11 |
GO - Molecular functioni
- alcohol dehydrogenase (NADP+) activity Source: GO_Central
- alditol:NADP+ 1-oxidoreductase activity Source: GO_Central
- androsterone dehydrogenase (B-specific) activity Source: UniProtKB-EC
- androsterone dehydrogenase activity Source: GO_Central
- bile acid binding Source: GO_Central
- ketosteroid monooxygenase activity Source: GO_Central
- oxidoreductase activity Source: GO_Central
- steroid binding Source: GO_Central
- steroid dehydrogenase activity Source: RGD
GO - Biological processi
- C21-steroid hormone metabolic process Source: GO_Central
- daunorubicin metabolic process Source: GO_Central
- doxorubicin metabolic process Source: GO_Central
- hippocampus development Source: RGD
- progesterone metabolic process Source: GO_Central
- prostaglandin metabolic process Source: GO_Central
- steroid metabolic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD, NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-14305 |
BRENDAi | 1.1.1.213, 5301 1.6.5.10, 5301 |
Reactomei | R-RNO-193368, Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-RNO-193775, Synthesis of bile acids and bile salts via 24-hydroxycholesterol R-RNO-193807, Synthesis of bile acids and bile salts via 27-hydroxycholesterol R-RNO-975634, Retinoid metabolism and transport |
SABIO-RKi | P23457 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.50)Short name: 3-alpha-HSD Alternative name(s): Hydroxyprostaglandin dehydrogenase |
Gene namesi | Name:Akr1c9 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 708361, LOC191574 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000124654 | 1 – 322 | 3-alpha-hydroxysteroid dehydrogenaseAdd BLAST | 322 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | Blocked amino end (Met) | 1 |
Proteomic databases
jPOSTi | P23457 |
PaxDbi | P23457 |
PRIDEi | P23457 |
PTM databases
iPTMneti | P23457 |
PhosphoSitePlusi | P23457 |
Expressioni
Tissue specificityi
In brain, highest levels found in olfactory bulb. Moderate levels present in cerebellum, cerebral cortex, hypothalamus and pituitary. Low levels present in amygdala, brain stem, caudate putamen, cingulate cortex, hippocampus, midbrain, and thalamus.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000017672, Expressed in liver and 21 other tissues |
Genevisiblei | P23457, RN |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000023835 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23457 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23457 |
Family & Domainsi
Sequence similaritiesi
Belongs to the aldo/keto reductase family.Curated
Phylogenomic databases
eggNOGi | KOG1577, Eukaryota |
GeneTreei | ENSGT00940000153677 |
HOGENOMi | CLU_023205_0_0_1 |
InParanoidi | P23457 |
OMAi | NNAVMDC |
OrthoDBi | 1016440at2759 |
PhylomeDBi | P23457 |
TreeFami | TF106492 |
Family and domain databases
CDDi | cd06660, Aldo_ket_red, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR018170, Aldo/ket_reductase_CS IPR020471, Aldo/keto_reductase IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR11732, PTHR11732, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit PS00063, ALDOKETO_REDUCTASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P23457-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDSISLRVAL NDGNFIPVLG FGTTVPEKVA KDEVIKATKI AIDNGFRHFD
60 70 80 90 100
SAYLYEVEEE VGQAIRSKIE DGTVKREDIF YTSKLWSTFH RPELVRTCLE
110 120 130 140 150
KTLKSTQLDY VDLYIIHFPM ALQPGDIFFP RDEHGKLLFE TVDICDTWEA
160 170 180 190 200
MEKCKDAGLA KSIGVSNFNC RQLERILNKP GLKYKPVCNQ VECHLYLNQS
210 220 230 240 250
KMLDYCKSKD IILVSYCTLG SSRDKTWVDQ KSPVLLDDPV LCAIAKKYKQ
260 270 280 290 300
TPALVALRYQ LQRGVVPLIR SFNAKRIKEL TQVFEFQLAS EDMKALDGLN
310 320
RNFRYNNAKY FDDHPNHPFT DE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 108 | L → Q in AAB19918 (PubMed:1922097).Curated | 1 | |
Sequence conflicti | 273 – 274 | NA → KP in AAB19918 (PubMed:1922097).Curated | 2 | |
Sequence conflicti | 280 | L → P in AAB19918 (PubMed:1922097).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64393 mRNA Translation: AAA40605.1 M61937 mRNA Translation: AAA41077.1 D17310 mRNA Translation: BAA04132.1 S57790 mRNA Translation: AAB19918.1 AF180334 , AF180326, AF180327, AF180328, AF180329, AF180330, AF180331, AF180332, AF180333 Genomic DNA Translation: AAF25813.1 BC091123 mRNA Translation: AAH91123.1 S35751 mRNA Translation: AAB21512.1 S35752 mRNA Translation: AAB21513.1 |
PIRi | A39350 PC2175 |
RefSeqi | NP_612556.1, NM_138547.3 |
Genome annotation databases
Ensembli | ENSRNOT00000023835; ENSRNOP00000023835; ENSRNOG00000017672 |
GeneIDi | 191574 |
KEGGi | rno:191574 |
UCSCi | RGD:708361, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64393 mRNA Translation: AAA40605.1 M61937 mRNA Translation: AAA41077.1 D17310 mRNA Translation: BAA04132.1 S57790 mRNA Translation: AAB19918.1 AF180334 , AF180326, AF180327, AF180328, AF180329, AF180330, AF180331, AF180332, AF180333 Genomic DNA Translation: AAF25813.1 BC091123 mRNA Translation: AAH91123.1 S35751 mRNA Translation: AAB21512.1 S35752 mRNA Translation: AAB21513.1 |
PIRi | A39350 PC2175 |
RefSeqi | NP_612556.1, NM_138547.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AFS | X-ray | 2.50 | A/B | 1-322 | [»] | |
1LWI | X-ray | 2.70 | A/B | 1-322 | [»] | |
1RAL | X-ray | 3.00 | A | 1-308 | [»] | |
SMRi | P23457 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000023835 |
Chemistry databases
ChEMBLi | CHEMBL1075221 |
PTM databases
iPTMneti | P23457 |
PhosphoSitePlusi | P23457 |
Proteomic databases
jPOSTi | P23457 |
PaxDbi | P23457 |
PRIDEi | P23457 |
Genome annotation databases
Ensembli | ENSRNOT00000023835; ENSRNOP00000023835; ENSRNOG00000017672 |
GeneIDi | 191574 |
KEGGi | rno:191574 |
UCSCi | RGD:708361, rat |
Organism-specific databases
CTDi | 105387 |
RGDi | 708361, LOC191574 |
Phylogenomic databases
eggNOGi | KOG1577, Eukaryota |
GeneTreei | ENSGT00940000153677 |
HOGENOMi | CLU_023205_0_0_1 |
InParanoidi | P23457 |
OMAi | NNAVMDC |
OrthoDBi | 1016440at2759 |
PhylomeDBi | P23457 |
TreeFami | TF106492 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-14305 |
BRENDAi | 1.1.1.213, 5301 1.6.5.10, 5301 |
Reactomei | R-RNO-193368, Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-RNO-193775, Synthesis of bile acids and bile salts via 24-hydroxycholesterol R-RNO-193807, Synthesis of bile acids and bile salts via 27-hydroxycholesterol R-RNO-975634, Retinoid metabolism and transport |
SABIO-RKi | P23457 |
Miscellaneous databases
EvolutionaryTracei | P23457 |
PROi | PR:P23457 |
Gene expression databases
Bgeei | ENSRNOG00000017672, Expressed in liver and 21 other tissues |
Genevisiblei | P23457, RN |
Family and domain databases
CDDi | cd06660, Aldo_ket_red, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR018170, Aldo/ket_reductase_CS IPR020471, Aldo/keto_reductase IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR11732, PTHR11732, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit PS00063, ALDOKETO_REDUCTASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DIDH_RAT | |
Accessioni | P23457Primary (citable) accession number: P23457 Secondary accession number(s): Q5BKC8, Q6LDE6, Q6LDE7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | October 7, 2020 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families