UniProtKB - P23441 (NKX21_RAT)
Protein
Homeobox protein Nkx-2.1
Gene
Nkx2-1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation (By similarity). Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (PubMed:22356123).By similarity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 161 – 220 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- core promoter sequence-specific DNA binding Source: CAFA
- DNA binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: RGD
- enzyme binding Source: RGD
- identical protein binding Source: CAFA
- intronic transcription regulatory region sequence-specific DNA binding Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: RGD
- TBP-class protein binding Source: CAFA
- transcription regulatory region sequence-specific DNA binding Source: RGD
GO - Biological processi
- anatomical structure formation involved in morphogenesis Source: RGD
- animal organ morphogenesis Source: RGD
- axon guidance Source: RGD
- brain development Source: RGD
- cell differentiation Source: GO_Central
- cellular response to leptin stimulus Source: RGD
- cerebral cortex cell migration Source: RGD
- cerebral cortex GABAergic interneuron differentiation Source: RGD
- cerebral cortex neuron differentiation Source: RGD
- circadian rhythm Source: UniProtKB
- club cell differentiation Source: RGD
- developmental induction Source: RGD
- development of primary female sexual characteristics Source: RGD
- embryonic lung development Source: RGD
- endoderm development Source: RGD
- epithelial tube branching involved in lung morphogenesis Source: RGD
- feeding behavior Source: RGD
- forebrain development Source: RGD
- forebrain dorsal/ventral pattern formation Source: RGD
- forebrain neuron differentiation Source: RGD
- forebrain neuron fate commitment Source: RGD
- globus pallidus development Source: RGD
- hippocampus development Source: RGD
- hyperosmotic salinity response Source: RGD
- Leydig cell differentiation Source: RGD
- locomotory behavior Source: RGD
- lung development Source: RGD
- lung saccule development Source: RGD
- negative regulation of cell migration Source: RGD
- negative regulation of epithelial to mesenchymal transition Source: RGD
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: RGD
- negative regulation of transforming growth factor beta receptor signaling pathway Source: RGD
- neuron fate commitment Source: RGD
- neuron migration Source: RGD
- oligodendrocyte differentiation Source: RGD
- pattern specification process Source: RGD
- phospholipid metabolic process Source: RGD
- pituitary gland development Source: RGD
- positive regulation of circadian rhythm Source: UniProtKB
- positive regulation of gene expression Source: RGD
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of blood volume by renin-angiotensin Source: RGD
- regulation of transcription, DNA-templated Source: RGD
- regulation of transcription by RNA polymerase II Source: RGD
- response to ethanol Source: RGD
- response to hormone Source: RGD
- response to lipopolysaccharide Source: RGD
- telencephalon cell migration Source: RGD
- telencephalon development Source: RGD
- thyroid gland development Source: RGD
- type II pneumocyte differentiation Source: RGD
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Homeobox protein Nkx-2.1Alternative name(s): Thyroid nuclear factor 1 Thyroid transcription factor 1 Short name: TTF-1 |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3866, Nkx2-1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleoplasm Source: UniProtKB
- nucleus Source: CAFA
Other locations
- transcription regulator complex Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049345 | 1 – 372 | Homeobox protein Nkx-2.1Add BLAST | 372 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 255 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated on serine residues by STK3/MST2.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P23441 |
PTM databases
iPTMneti | P23441 |
PhosphoSitePlusi | P23441 |
Expressioni
Tissue specificityi
Thyroid, lung and CNS. Expressed in restricted regions of the developing brain within the diencephalon, in parts of the hypothalamus and neurohypophysis, and in the telencephalon.
Inductioni
Expression oscillates in a diurnal and melatonin-dependent fashion in the preoptic area (POA) region in the hypothalamus, with maximal expression attained during the dark phase of the light/dark cycle.1 Publication
Interactioni
Subunit structurei
Interacts with WWTR1.
By similarityBinary interactionsi
Hide detailsP23441
With | #Exp. | IntAct |
---|---|---|
Pax8 [P51974] | 5 | EBI-1223127,EBI-1223113 |
Wwtr1 [Q9EPK5] from Mus musculus. | 3 | EBI-1223127,EBI-1211920 |
GO - Molecular functioni
- enzyme binding Source: RGD
- identical protein binding Source: CAFA
- TBP-class protein binding Source: CAFA
Protein-protein interaction databases
IntActi | P23441, 2 interactors |
MINTi | P23441 |
STRINGi | 10116.ENSRNOP00000011453 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P23441 |
SMRi | P23441 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23441 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 234 – 244 | Poly-GlyAdd BLAST | 11 | |
Compositional biasi | 247 – 254 | Poly-Gln | 8 | |
Compositional biasi | 295 – 304 | Poly-Ala | 10 |
Sequence similaritiesi
Belongs to the NK-2 homeobox family.Curated
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0842, Eukaryota |
InParanoidi | P23441 |
PhylomeDBi | P23441 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
DisProti | DP00071 |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P23441-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSMSPKHTTP FSVSDILSPL EESYKKVGME GGGLGAPLAA YRQGQAAPPA
60 70 80 90 100
AAMQQHAVGH HGAVTAAYHM TAAGVPQLSH SAVGGYCNGN LGNMSELPPY
110 120 130 140 150
QDTMRNSASG PGWYGANPDP RFPAISRFMG PASGMNMSGM GGLGSLGDVS
160 170 180 190 200
KNMAPLPSAP RRKRRVLFSQ AQVYELERRF KQQKYLSAPE REHLASMIHL
210 220 230 240 250
TPTQVKIWFQ NHRYKMKRQA KDKAAQQQLQ QDSGGGGGGG GGAGCPQQQQ
260 270 280 290 300
AQQQSPRRVA VPVLVKDGKP CQAGAPAPGA ASLQGHAQQQ AQQQAQAAQA
310 320 330 340 350
AAAAISVGSG GAGLGAHPGH QPGSAGQSPD LAHHAASPAA LQGQVSSLSH
360 370
LNSSGSDYGA MSCSTLLYGR TW
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V740 | G3V740_RAT | Homeobox protein Nkx-2.1 | Nkx2-1 rCG_62381 | 372 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53858 mRNA Translation: CAA37851.1 D38035 Genomic DNA Translation: BAA07231.1 Sequence problems. |
PIRi | S12002 |
Genome annotation databases
UCSCi | RGD:3866, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53858 mRNA Translation: CAA37851.1 D38035 Genomic DNA Translation: BAA07231.1 Sequence problems. |
PIRi | S12002 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FTT | NMR | - | A | 161-227 | [»] | |
BMRBi | P23441 | |||||
SMRi | P23441 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P23441, 2 interactors |
MINTi | P23441 |
STRINGi | 10116.ENSRNOP00000011453 |
PTM databases
iPTMneti | P23441 |
PhosphoSitePlusi | P23441 |
Proteomic databases
PaxDbi | P23441 |
Genome annotation databases
UCSCi | RGD:3866, rat |
Organism-specific databases
RGDi | 3866, Nkx2-1 |
Phylogenomic databases
eggNOGi | KOG0842, Eukaryota |
InParanoidi | P23441 |
PhylomeDBi | P23441 |
Miscellaneous databases
EvolutionaryTracei | P23441 |
PROi | PR:P23441 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
DisProti | DP00071 |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NKX21_RAT | |
Accessioni | P23441Primary (citable) accession number: P23441 Secondary accession number(s): O08630, O70121 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | October 7, 2020 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families