UniProtKB - P23440 (PDE6B_MOUSE)
Protein
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
Gene
Pde6b
Organism
Mus musculus (Mouse)
Status
Functioni
Necessary for the formation of a functional phosphodiesterase holoenzyme (PubMed:1847109). Involved in retinal circadian rhythm photoentrainment via modulation of UVA and orange light-induced phase-shift of the retina clock (PubMed:30240620). May participate in processes of transmission and amplification of the visual signal (By similarity).By similarity2 Publications
Catalytic activityi
- EC:3.1.4.35
Cofactori
a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 557 | Proton donorBy similarity | 1 | |
Metal bindingi | 561 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 597 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 598 | Divalent metal cation 1By similarity | 1 | |
Metal bindingi | 598 | Divalent metal cation 2By similarity | 1 | |
Metal bindingi | 718 | Divalent metal cation 1By similarity | 1 |
GO - Molecular functioni
- 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
- 3',5'-cyclic-nucleotide phosphodiesterase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- detection of light stimulus Source: MGI
- entrainment of circadian clock by photoperiod Source: UniProtKB
- retina development in camera-type eye Source: MGI
- signal transduction Source: GO_Central
- visual perception Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Sensory transduction, Vision |
Ligand | cGMP, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2485179, Activation of the phototransduction cascade R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-MMU-4086398, Ca2+ pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta (EC:3.1.4.35)Short name: GMP-PDE beta |
Gene namesi | Name:Pde6b Synonyms:Mpb, Pdeb, rd |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97525, Pde6b |
Subcellular locationi
Other locations
Plasma Membrane
- photoreceptor outer segment membrane Source: GO_Central
- plasma membrane Source: Reactome
Other locations
- photoreceptor outer segment Source: MGI
Keywords - Cellular componenti
MembranePathology & Biotechi
Involvement in diseasei
Defects in Pde6b are the cause of retinal degeneration.1 Publication
Disruption phenotypei
Abolishes the retinal photoreceptor layer, outer nuclear layer, and outer plexiform layer in the retinal ultrastructure (PubMed:30240620). Loss of Opn1sw, Opn1mw and Rho expression in the retina (PubMed:30240620). Reduced rate of circadian photoentrainment, UVA and orange light-induced phase-shift response and Fos expression in the suprachiasmatic nuclei (SCN) in the brain (PubMed:30240620). Pde6b and Opn5 double knockout mice also show loss of retinal ultrastructures and a more severe reduction in the rate of circadian photoentrainment, light-induced phase-shift response and Fos expression in the SCN (PubMed:30240620).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000023350 | 2 – 853 | Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit betaAdd BLAST | 852 | |
PropeptideiPRO_0000023351 | 854 – 856 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Lipidationi | 853 | S-geranylgeranyl cysteineBy similarity | 1 |
Keywords - PTMi
Acetylation, Lipoprotein, PrenylationProteomic databases
MaxQBi | P23440 |
PaxDbi | P23440 |
PRIDEi | P23440 |
PTM databases
iPTMneti | P23440 |
PhosphoSitePlusi | P23440 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029491, Expressed in retina and 72 other tissues |
Genevisiblei | P23440, MM |
Interactioni
Subunit structurei
Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
Protein-protein interaction databases
BioGRIDi | 202084, 20 interactors |
STRINGi | 10090.ENSMUSP00000031456 |
Miscellaneous databases
RNActi | P23440, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 71 – 220 | GAF 1Add BLAST | 150 | |
Domaini | 252 – 429 | GAF 2Add BLAST | 178 | |
Domaini | 481 – 814 | PDEasePROSITE-ProRule annotationAdd BLAST | 334 |
Sequence similaritiesi
Belongs to the cyclic nucleotide phosphodiesterase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3689, Eukaryota |
GeneTreei | ENSGT00940000156471 |
HOGENOMi | CLU_006980_2_0_1 |
InParanoidi | P23440 |
OMAi | CDEDELG |
OrthoDBi | 904682at2759 |
TreeFami | TF316499 |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit 3.30.450.40, 2 hits |
InterProi | View protein in InterPro IPR003018, GAF IPR029016, GAF-like_dom_sf IPR003607, HD/PDEase_dom IPR023088, PDEase IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS |
Pfami | View protein in Pfam PF01590, GAF, 2 hits PF00233, PDEase_I, 1 hit |
PRINTSi | PR00387, PDIESTERASE1 |
SMARTi | View protein in SMART SM00065, GAF, 2 hits SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: P23440-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSLSEEQVRS FLDGNPTFAH QYFGKKLSPE NVAGACEDGW LADCGSLREL
60 70 80 90 100
CQVEESAALF ELVQDMQESV NMERVVFKIL RRLCTILHAD RCSLFMYRQR
110 120 130 140 150
NGIAELATRL FSVQPDSLLE DCLVPPDSEI VFPLDIGIVG HVAQTKKMIN
160 170 180 190 200
VQDVAECPHF SSFADELTDY VTKNILSTPI MNGKDVVAVI MAVNKLDGPC
210 220 230 240 250
FTSEDEDVFT KYLNFATLNL KIYHLSYLHN CETRRGQVLL WSANKVFEEL
260 270 280 290 300
TDIERQFHKA FYTVRAYLNC ERYSVGLLDM TKEKEFFDVW PVLMGEAQPY
310 320 330 340 350
SGPRTPDGRE IVFYKVIDYI LHGKEDIKVI PTPPADHWAL ASGLPTYVAE
360 370 380 390 400
SGFICNIMNA SADEMFNFQE GPLDDSGWVI KNVLSMPIVN KKEEIVGVAT
410 420 430 440 450
FYNRKDGKPF DDQDEVLMES LTQFLGWSVL NTDTYDKMNK LENRKDIAQD
460 470 480 490 500
MVLYHVRCDK DEIQEILPTR DRLGKEPADC EEDELGKILK EELPGPTKFD
510 520 530 540 550
IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL VRFLFSVSKA
560 570 580 590 600
YRRITYHNWR HGFNVAQTMF TLLMTGKLKS YYTDLEAFAM VTAGLCHDID
610 620 630 640 650
HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLAEE SLNIYQNLNR
660 670 680 690 700
RQHEHVIHLM DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DKKSWVEYLS
710 720 730 740 750
LETTRKEIVM AMMMTACDLS AITKPWEVQS KVALLVAAEF WEQGDLERTV
760 770 780 790 800
LDQQPIPMMD RNKAAELPKL QVGFIDFVCT FVYKEFSRFH EEILPMFDRL
810 820 830 840 850
QNNRKEWKAL ADEYEAKVKA LEEEKKKEED RVAAKKVGTE VCNGGPAPKS
STCCIL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | E → G in CAA39439 (PubMed:1977087).Curated | 1 | |
Sequence conflicti | 19 | A → S in CAA39439 (PubMed:1977087).Curated | 1 | |
Sequence conflicti | 49 – 50 | EL → DV in CAA39439 (PubMed:1977087).Curated | 2 | |
Sequence conflicti | 158 | P → T in CAA39439 (PubMed:1977087).Curated | 1 | |
Sequence conflicti | 176 | L → C in CAA39439 (PubMed:1977087).Curated | 1 | |
Sequence conflicti | 232 | E → R in CAA39439 (PubMed:1977087).Curated | 1 | |
Sequence conflicti | 236 | G → S in CAA39439 (PubMed:1977087).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004592 | 801 – 856 | Missing in isoform 2. CuratedAdd BLAST | 56 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55968 mRNA Translation: CAA39439.1 X60133 mRNA Translation: CAA42719.1 AK044364 mRNA Translation: BAC31885.1 CH466529 Genomic DNA Translation: EDL20122.1 |
CCDSi | CCDS19510.1 [P23440-1] |
PIRi | S30762 |
RefSeqi | NP_032832.2, NM_008806.2 [P23440-1] |
Genome annotation databases
Ensembli | ENSMUST00000031456; ENSMUSP00000031456; ENSMUSG00000029491 [P23440-1] |
GeneIDi | 18587 |
KEGGi | mmu:18587 |
UCSCi | uc008ynz.1, mouse [P23440-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55968 mRNA Translation: CAA39439.1 X60133 mRNA Translation: CAA42719.1 AK044364 mRNA Translation: BAC31885.1 CH466529 Genomic DNA Translation: EDL20122.1 |
CCDSi | CCDS19510.1 [P23440-1] |
PIRi | S30762 |
RefSeqi | NP_032832.2, NM_008806.2 [P23440-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 202084, 20 interactors |
STRINGi | 10090.ENSMUSP00000031456 |
PTM databases
iPTMneti | P23440 |
PhosphoSitePlusi | P23440 |
Proteomic databases
MaxQBi | P23440 |
PaxDbi | P23440 |
PRIDEi | P23440 |
Protocols and materials databases
Antibodypediai | 8018, 103 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000031456; ENSMUSP00000031456; ENSMUSG00000029491 [P23440-1] |
GeneIDi | 18587 |
KEGGi | mmu:18587 |
UCSCi | uc008ynz.1, mouse [P23440-1] |
Organism-specific databases
CTDi | 5158 |
MGIi | MGI:97525, Pde6b |
Phylogenomic databases
eggNOGi | KOG3689, Eukaryota |
GeneTreei | ENSGT00940000156471 |
HOGENOMi | CLU_006980_2_0_1 |
InParanoidi | P23440 |
OMAi | CDEDELG |
OrthoDBi | 904682at2759 |
TreeFami | TF316499 |
Enzyme and pathway databases
Reactomei | R-MMU-2485179, Activation of the phototransduction cascade R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-MMU-4086398, Ca2+ pathway |
Miscellaneous databases
BioGRID-ORCSi | 18587, 2 hits in 17 CRISPR screens |
ChiTaRSi | Pde6b, mouse |
PROi | PR:P23440 |
RNActi | P23440, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029491, Expressed in retina and 72 other tissues |
Genevisiblei | P23440, MM |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
Gene3Di | 1.10.1300.10, 1 hit 3.30.450.40, 2 hits |
InterProi | View protein in InterPro IPR003018, GAF IPR029016, GAF-like_dom_sf IPR003607, HD/PDEase_dom IPR023088, PDEase IPR002073, PDEase_catalytic_dom IPR036971, PDEase_catalytic_dom_sf IPR023174, PDEase_CS |
Pfami | View protein in Pfam PF01590, GAF, 2 hits PF00233, PDEase_I, 1 hit |
PRINTSi | PR00387, PDIESTERASE1 |
SMARTi | View protein in SMART SM00065, GAF, 2 hits SM00471, HDc, 1 hit |
PROSITEi | View protein in PROSITE PS00126, PDEASE_I_1, 1 hit PS51845, PDEASE_I_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDE6B_MOUSE | |
Accessioni | P23440Primary (citable) accession number: P23440 Secondary accession number(s): Q80UF0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families