UniProtKB - P23381 (SYWC_HUMAN)
Tryptophan--tRNA ligase, cytoplasmic
WARS1
Functioni
Catalytic activityi
- EC:6.1.1.22 Publications
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kinase inhibitor activity Source: CAFA
- protein domain specific binding Source: CAFA
- protein homodimerization activity Source: UniProtKB
- protein kinase binding Source: CAFA
- tryptophan-tRNA ligase activity Source: UniProtKB
GO - Biological processi
- angiogenesis Source: UniProtKB-KW
- negative regulation of cell population proliferation Source: ProtInc
- negative regulation of protein kinase activity Source: CAFA
- negative regulation of protein phosphorylation Source: CAFA
- positive regulation of gene expression Source: CAFA
- positive regulation of protein-containing complex assembly Source: CAFA
- regulation of angiogenesis Source: UniProtKB
- regulation of protein ADP-ribosylation Source: CAFA
- translation Source: ProtInc
- tRNA aminoacylation for protein translation Source: Reactome
- tryptophanyl-tRNA aminoacylation Source: GO_Central
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Angiogenesis, Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.1.1.2, 2681 |
PathwayCommonsi | P23381 |
Reactomei | R-HSA-379716, Cytosolic tRNA aminoacylation |
Protein family/group databases
MoonProti | P23381 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12729, WARS1 |
MIMi | 191050, gene |
neXtProti | NX_P23381 |
VEuPathDBi | HostDB:ENSG00000140105.17 |
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleus Source: CAFA
Other locations
- cytoplasm Source: GO_Central
- protein-containing complex Source: CAFA
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Involvement in diseasei
Neuronopathy, distal hereditary motor, 9 (HMN9)1 Publication
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_080407 | 257 | H → R in HMN9; decreased tryptophan-tRNA ligase activity; dominant negative effect; decreased general protein synthesis; decreased cell viability; no effect on homodimerization. 1 Publication | 1 |
Keywords - Diseasei
Disease variant, Neurodegeneration, NeuropathyOrganism-specific databases
DisGeNETi | 7453 |
GeneReviewsi | WARS1 |
MalaCardsi | WARS1 |
MIMi | 617721, phenotype |
OpenTargetsi | ENSG00000140105 |
PharmGKBi | PA37340 |
Miscellaneous databases
Pharosi | P23381, Tbio |
Chemistry databases
DrugBanki | DB00150, Tryptophan DB04537, Tryptophanamide DB01831, Tryptophanyl-5'amp |
Genetic variation databases
BioMutai | WARS |
DMDMi | 135191 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources2 Publications | |||
ChainiPRO_0000136738 | 2 – 471 | Tryptophan--tRNA ligase, cytoplasmicAdd BLAST | 470 | |
ChainiPRO_0000386461 | 71 – 471 | T1-TrpRSAdd BLAST | 401 | |
ChainiPRO_0000386462 | 94 – 471 | T2-TrpRSAdd BLAST | 378 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 154 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 351 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P23381 |
jPOSTi | P23381 |
MassIVEi | P23381 |
MaxQBi | P23381 |
PaxDbi | P23381 |
PeptideAtlasi | P23381 |
PRIDEi | P23381 |
ProteomicsDBi | 54085 [P23381-1] 54086 [P23381-2] |
2D gel databases
OGPi | P23381 |
PTM databases
iPTMneti | P23381 |
MetOSitei | P23381 |
PhosphoSitePlusi | P23381 |
SwissPalmi | P23381 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSG00000140105, Expressed in epithelium of bronchus and 248 other tissues |
ExpressionAtlasi | P23381, baseline and differential |
Genevisiblei | P23381, HS |
Organism-specific databases
HPAi | ENSG00000140105, Tissue enhanced (blood, thyroid gland) |
Interactioni
Subunit structurei
Homodimer (PubMed:28369220). Isoform 1 and isoform 2 interact with an oxidized form of GAPDH. GAPDH stimulates the aminoacylation activity of isoform 2 (PubMed:15628863).
2 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- protein domain specific binding Source: CAFA
- protein homodimerization activity Source: UniProtKB
- protein kinase binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 113292, 85 interactors |
DIPi | DIP-29493N |
IntActi | P23381, 18 interactors |
MINTi | P23381 |
STRINGi | 9606.ENSP00000347495 |
Miscellaneous databases
RNActi | P23381, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P23381 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23381 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 64 | WHEP-TRSPROSITE-ProRule annotationAdd BLAST | 57 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 164 – 173 | 'HIGH' region | 10 | |
Motifi | 349 – 353 | 'KMSKS' region | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2145, Eukaryota |
GeneTreei | ENSGT00940000153724 |
HOGENOMi | CLU_032621_0_1_1 |
InParanoidi | P23381 |
OMAi | SIYHRFM |
PhylomeDBi | P23381 |
TreeFami | TF105669 |
Family and domain databases
CDDi | cd00806, TrpRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002305, aa-tRNA-synth_Ic IPR014729, Rossmann-like_a/b/a_fold IPR009068, S15_NS1_RNA-bd IPR002306, Trp-tRNA-ligase IPR000738, WHEP-TRS_dom |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit PF00458, WHEP-TRS, 1 hit |
PRINTSi | PR01039, TRNASYNTHTRP |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit |
TIGRFAMsi | TIGR00233, trpS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 26 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPNSEPASLL ELFNSIATQG ELVRSLKAGN ASKDEIDSAV KMLVSLKMSY
60 70 80 90 100
KAAAGEDYKA DCPPGNPAPT SNHGPDATEA EEDFVDPWTV QTSSAKGIDY
110 120 130 140 150
DKLIVRFGSS KIDKELINRI ERATGQRPHH FLRRGIFFSH RDMNQVLDAY
160 170 180 190 200
ENKKPFYLYT GRGPSSEAMH VGHLIPFIFT KWLQDVFNVP LVIQMTDDEK
210 220 230 240 250
YLWKDLTLDQ AYSYAVENAK DIIACGFDIN KTFIFSDLDY MGMSSGFYKN
260 270 280 290 300
VVKIQKHVTF NQVKGIFGFT DSDCIGKISF PAIQAAPSFS NSFPQIFRDR
310 320 330 340 350
TDIQCLIPCA IDQDPYFRMT RDVAPRIGYP KPALLHSTFF PALQGAQTKM
360 370 380 390 400
SASDPNSSIF LTDTAKQIKT KVNKHAFSGG RDTIEEHRQF GGNCDVDVSF
410 420 430 440 450
MYLTFFLEDD DKLEQIRKDY TSGAMLTGEL KKALIEVLQP LIAEHQARRK
460 470
EVTDEIVKEF MTPRKLSFDF Q
Computationally mapped potential isoform sequencesi
There are 26 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V3H8 | G3V3H8_HUMAN | Tryptophanyl-tRNA synthetase | WARS1 | 223 | Annotation score: | ||
G3V3Y5 | G3V3Y5_HUMAN | Tryptophanyl-tRNA synthetase | WARS1 | 226 | Annotation score: | ||
H0YJP3 | H0YJP3_HUMAN | Tryptophanyl-tRNA synthetase | WARS1 | 185 | Annotation score: | ||
G3V277 | G3V277_HUMAN | Tryptophan--tRNA ligase, cytoplasmi... | WARS1 | 212 | Annotation score: | ||
G3V456 | G3V456_HUMAN | Tryptophan--tRNA ligase, cytoplasmi... | WARS1 | 154 | Annotation score: | ||
G3V5U1 | G3V5U1_HUMAN | Tryptophan--tRNA ligase, cytoplasmi... | WARS1 | 153 | Annotation score: | ||
G3V2Y7 | G3V2Y7_HUMAN | Tryptophan--tRNA ligase, cytoplasmi... | WARS1 | 177 | Annotation score: | ||
G3V227 | G3V227_HUMAN | Tryptophan--tRNA ligase, cytoplasmi... | WARS1 | 152 | Annotation score: | ||
G3V3P2 | G3V3P2_HUMAN | Tryptophan--tRNA ligase, cytoplasmi... | WARS1 | 150 | Annotation score: | ||
G3V3S7 | G3V3S7_HUMAN | Tryptophan--tRNA ligase, cytoplasmi... | WARS1 | 114 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 213 – 214 | SY → GD in AAA61298 (PubMed:1765274).Curated | 2 | |
Sequence conflicti | 310 | A → V in AAH95453 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 424 | A → R in CAA44450 (PubMed:1537332).Curated | 1 | |
Sequence conflicti | 439 | Q → L in AAH95453 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052406 | 54 | A → S. Corresponds to variant dbSNP:rs2234521Ensembl. | 1 | |
Natural variantiVAR_080407 | 257 | H → R in HMN9; decreased tryptophan-tRNA ligase activity; dominant negative effect; decreased general protein synthesis; decreased cell viability; no effect on homodimerization. 1 Publication | 1 | |
Natural variantiVAR_080408 | 443 | A → S1 PublicationCorresponds to variant dbSNP:rs139914390Ensembl. | 1 | |
Natural variantiVAR_036466 | 455 | E → D in a breast cancer sample; somatic mutation. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038221 | 1 – 41 | Missing in isoform 2. 1 PublicationAdd BLAST | 41 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000344102; ENSP00000339485; ENSG00000140105 [P23381-2] ENST00000355338; ENSP00000347495; ENSG00000140105 [P23381-1] ENST00000358655; ENSP00000351481; ENSG00000140105 [P23381-2] ENST00000392882; ENSP00000376620; ENSG00000140105 [P23381-1] ENST00000556645; ENSP00000451887; ENSG00000140105 [P23381-2] ENST00000557135; ENSP00000451460; ENSG00000140105 [P23381-1] |
GeneIDi | 7453 |
KEGGi | hsa:7453 |
UCSCi | uc001yhg.3, human [P23381-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1O5T | X-ray | 2.50 | A | 94-471 | [»] | |
1R6T | X-ray | 2.10 | A/B | 1-466 | [»] | |
1R6U | X-ray | 2.00 | A/B | 48-471 | [»] | |
1ULH | X-ray | 2.31 | A/B | 82-471 | [»] | |
2AKE | X-ray | 3.10 | A | 94-471 | [»] | |
2AZX | X-ray | 2.80 | A/B | 1-471 | [»] | |
2DR2 | X-ray | 3.00 | A | 94-471 | [»] | |
2QUH | X-ray | 2.40 | A/B | 1-471 | [»] | |
2QUI | X-ray | 2.40 | A/B | 1-471 | [»] | |
2QUJ | X-ray | 2.42 | A/B | 1-471 | [»] | |
2QUK | X-ray | 2.80 | A | 1-471 | [»] | |
5UJI | X-ray | 2.79 | A/B | 97-471 | [»] | |
5UJJ | X-ray | 2.10 | A/B | 1-471 | [»] | |
SMRi | P23381 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113292, 85 interactors |
DIPi | DIP-29493N |
IntActi | P23381, 18 interactors |
MINTi | P23381 |
STRINGi | 9606.ENSP00000347495 |
Chemistry databases
DrugBanki | DB00150, Tryptophan DB04537, Tryptophanamide DB01831, Tryptophanyl-5'amp |
Protein family/group databases
MoonProti | P23381 |
PTM databases
iPTMneti | P23381 |
MetOSitei | P23381 |
PhosphoSitePlusi | P23381 |
SwissPalmi | P23381 |
Genetic variation databases
BioMutai | WARS |
DMDMi | 135191 |
2D gel databases
OGPi | P23381 |
Proteomic databases
EPDi | P23381 |
jPOSTi | P23381 |
MassIVEi | P23381 |
MaxQBi | P23381 |
PaxDbi | P23381 |
PeptideAtlasi | P23381 |
PRIDEi | P23381 |
ProteomicsDBi | 54085 [P23381-1] 54086 [P23381-2] |
Protocols and materials databases
ABCDi | P23381, 4 sequenced antibodies |
Antibodypediai | 27522, 250 antibodies |
Genome annotation databases
Ensembli | ENST00000344102; ENSP00000339485; ENSG00000140105 [P23381-2] ENST00000355338; ENSP00000347495; ENSG00000140105 [P23381-1] ENST00000358655; ENSP00000351481; ENSG00000140105 [P23381-2] ENST00000392882; ENSP00000376620; ENSG00000140105 [P23381-1] ENST00000556645; ENSP00000451887; ENSG00000140105 [P23381-2] ENST00000557135; ENSP00000451460; ENSG00000140105 [P23381-1] |
GeneIDi | 7453 |
KEGGi | hsa:7453 |
UCSCi | uc001yhg.3, human [P23381-1] |
Organism-specific databases
CTDi | 7453 |
DisGeNETi | 7453 |
GeneCardsi | WARS1 |
GeneReviewsi | WARS1 |
HGNCi | HGNC:12729, WARS1 |
HPAi | ENSG00000140105, Tissue enhanced (blood, thyroid gland) |
MalaCardsi | WARS1 |
MIMi | 191050, gene 617721, phenotype |
neXtProti | NX_P23381 |
OpenTargetsi | ENSG00000140105 |
PharmGKBi | PA37340 |
VEuPathDBi | HostDB:ENSG00000140105.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2145, Eukaryota |
GeneTreei | ENSGT00940000153724 |
HOGENOMi | CLU_032621_0_1_1 |
InParanoidi | P23381 |
OMAi | SIYHRFM |
PhylomeDBi | P23381 |
TreeFami | TF105669 |
Enzyme and pathway databases
BRENDAi | 6.1.1.2, 2681 |
PathwayCommonsi | P23381 |
Reactomei | R-HSA-379716, Cytosolic tRNA aminoacylation |
Miscellaneous databases
BioGRID-ORCSi | 7453, 749 hits in 1005 CRISPR screens |
ChiTaRSi | WARS, human |
EvolutionaryTracei | P23381 |
GeneWikii | WARS_(gene) |
GenomeRNAii | 7453 |
Pharosi | P23381, Tbio |
PROi | PR:P23381 |
RNActi | P23381, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000140105, Expressed in epithelium of bronchus and 248 other tissues |
ExpressionAtlasi | P23381, baseline and differential |
Genevisiblei | P23381, HS |
Family and domain databases
CDDi | cd00806, TrpRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002305, aa-tRNA-synth_Ic IPR014729, Rossmann-like_a/b/a_fold IPR009068, S15_NS1_RNA-bd IPR002306, Trp-tRNA-ligase IPR000738, WHEP-TRS_dom |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit PF00458, WHEP-TRS, 1 hit |
PRINTSi | PR01039, TRNASYNTHTRP |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit |
TIGRFAMsi | TIGR00233, trpS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYWC_HUMAN | |
Accessioni | P23381Primary (citable) accession number: P23381 Secondary accession number(s): A6NGN1 Q9UDL3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | December 1, 1992 | |
Last modified: | April 7, 2021 | |
This is version 209 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families