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Entry version 142 (10 Feb 2021)
Sequence version 2 (11 Jul 2002)
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Protein

PTS system fructose-specific EIIB'BC component

Gene

fruA

Organism
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FruAB PTS system is involved in fructose transport.

By similarity1 Publication1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei126Phosphocysteine intermediate; for EIIB activityBy similarity1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
PTS system fructose-specific EIIB'BC component1 Publication
Alternative name(s):
EIIB'BC-Fru1 Publication
Including the following 2 domains:
PTS system fructose-specific EIIB component1 Publication (EC:2.7.1.202By similarity)
Alternative name(s):
EIII-FruBy similarity
Fructose-specific phosphotransferase enzyme IIB component1 Publication
PTS system fructose-specific EIIC component1 Publication
Alternative name(s):
Fructose permease IIC component1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:fruA1 Publication
Ordered Locus Names:XCC2372
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiXanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri190485 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001010 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei254 – 274HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei289 – 309HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei332 – 352HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei369 – 389HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei410 – 430HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei451 – 471HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei483 – 503HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei509 – 529HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei549 – 571HelicalPROSITE-ProRule annotationAdd BLAST23

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Cells lacking this gene grow normally on glucose but are unable to grow on fructose.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001865151 – 580PTS system fructose-specific EIIB'BC componentAdd BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei126Phosphocysteine; by EIIAPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

By fructose.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
340.xcc-b100_1800

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P23355

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1 – 99PTS EIIB type-2 1By similarity1 PublicationAdd BLAST99
Domaini120 – 215PTS EIIB type-2 2PROSITE-ProRule annotationAdd BLAST96
Domaini243 – 580PTS EIIC type-2PROSITE-ProRule annotationAdd BLAST338

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation
In the N-terminal, the PTS system fructose-specific possesses a duplicated EIIB domain (EIIB' domain) which lacks the active site and functions to facilitate phosphoryl transfer between the EIIA domain of diphosphoryl transfer protein (DTP) and the EIIB domain. The presence of the EIIB' domain is required for normal high affinity recognition of DTP by the PTS system fructose-specific as well as for normal rates of phosphoryl transfer between the EIIA and EIIB domains of DTP and PTS system fructose-specific, respectively.By similarity
The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1299, Bacteria
COG1445, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_013155_4_2_6

Identification of Orthologs from Complete Genome Data

More...
OMAi
EGMKPML

Family and domain databases

Conserved Domains Database

More...
CDDi
cd05569, PTS_IIB_fructose, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR036095, PTS_EIIB-like_sf
IPR013011, PTS_EIIB_2
IPR003501, PTS_EIIB_2/3
IPR013014, PTS_EIIC_2
IPR003353, PTS_IIB_fruc
IPR006327, PTS_IIC_fruc

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02302, PTS_IIB, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52794, SSF52794, 2 hits

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00829, FRU, 1 hit
TIGR01427, PTS_IIC_fructo, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51099, PTS_EIIB_TYPE_2, 1 hit
PS51104, PTS_EIIC_TYPE_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P23355-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSSIVVIAA GERSTEAVLA AEALRRAATA AGRSVTIEIR SDQGVLGALP
60 70 80 90 100
TELTNGAAHV LIVGDADADT ARFGDAQLLH LSLGAVLDDP AAAVSQLAAT
110 120 130 140 150
TAPASTSATT DASGAGGKRI VAITSCPTGI AHTFMAAEGL QQAAKKLGYQ
160 170 180 190 200
MRVETQGSVG AQDALTDEEI RAADVVIIAA DREVDLARFG GKRLFKSGTK
210 220 230 240 250
PAINDGPALI QKALAEAGVH GGAAPVAGAN ATSDAKGNAR TGAYKHLMTG
260 270 280 290 300
VSFMLPFVTA GGLLIALAFA LGGIYAGDDA HQGTLAWSLF QIGAKAGFTL
310 320 330 340 350
MVPALAGYIA YSIADRPGIA PGMIGGLVAA NLNAGFLGGI IAGFIAGYGV
360 370 380 390 400
AALNRYIKLP RNLEGLKPVL ILPVLGTLLV GLAMMYVFGQ PVADLLAWLT
410 420 430 440 450
AWLRGMQGSS ALLLGLLLGG MMAFDMGGPV NKAAYAFSTG LIASQVYTPM
460 470 480 490 500
AAAMVAGMTP PLGIALATWV FRNRFTVEER GSATAAGVLG LAFVTEGAIP
510 520 530 540 550
YAARDPLRTI PALVIGSAVA GAISMTAGAE LKAPHGGIFV LLIPNAVTHL
560 570 580
LNYVLALVVG VVVTAVALRL LKKPVADVIA
Length:580
Mass (Da):58,647
Last modified:July 11, 2002 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE6A3B52A7F45662B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti150Q → H in AAA27602 (PubMed:1655739).Curated1
Sequence conflicti260 – 264AGGLL → LAACW in AAA27602 (PubMed:1655739).Curated5
Sequence conflicti350V → L in AAA27602 (PubMed:1655739).Curated1
Sequence conflicti408 – 409GS → AG in AAA27602 (PubMed:1655739).Curated2
Sequence conflicti494 – 495VT → DS in AAA27602 (PubMed:1655739).Curated2
Sequence conflicti568L → V in AAA27602 (PubMed:1655739).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M69242 Genomic DNA Translation: AAA27602.1
AE008922 Genomic DNA Translation: AAM41650.1

Protein sequence database of the Protein Information Resource

More...
PIRi
B40944

NCBI Reference Sequences

More...
RefSeqi
NP_637726.1, NC_003902.1
WP_011037515.1, NC_003902.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAM41650; AAM41650; XCC2372

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
xcc:XCC2372

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|190485.4.peg.2526

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69242 Genomic DNA Translation: AAA27602.1
AE008922 Genomic DNA Translation: AAM41650.1
PIRiB40944
RefSeqiNP_637726.1, NC_003902.1
WP_011037515.1, NC_003902.1

3D structure databases

SMRiP23355
ModBaseiSearch...

Protein-protein interaction databases

STRINGi340.xcc-b100_1800

Genome annotation databases

EnsemblBacteriaiAAM41650; AAM41650; XCC2372
KEGGixcc:XCC2372
PATRICifig|190485.4.peg.2526

Phylogenomic databases

eggNOGiCOG1299, Bacteria
COG1445, Bacteria
HOGENOMiCLU_013155_4_2_6
OMAiEGMKPML

Family and domain databases

CDDicd05569, PTS_IIB_fructose, 1 hit
InterProiView protein in InterPro
IPR036095, PTS_EIIB-like_sf
IPR013011, PTS_EIIB_2
IPR003501, PTS_EIIB_2/3
IPR013014, PTS_EIIC_2
IPR003353, PTS_IIB_fruc
IPR006327, PTS_IIC_fruc
PfamiView protein in Pfam
PF02302, PTS_IIB, 1 hit
SUPFAMiSSF52794, SSF52794, 2 hits
TIGRFAMsiTIGR00829, FRU, 1 hit
TIGR01427, PTS_IIC_fructo, 1 hit
PROSITEiView protein in PROSITE
PS51099, PTS_EIIB_TYPE_2, 1 hit
PS51104, PTS_EIIC_TYPE_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPTFBC_XANCP
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P23355
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: July 11, 2002
Last modified: February 10, 2021
This is version 142 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome
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