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Protein

Thymidine kinase

Gene

tdk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Phosphorylates both thymidine and deoxyuridine.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Enzyme regulationi

Allosteric enzyme which is feedback inhibited by dTTP and activated by a number of dNDP and dNTP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Proton acceptorUniRule annotation1
Metal bindingi145ZincUniRule annotation1
Metal bindingi147ZincUniRule annotation1
Metal bindingi182ZincUniRule annotation1
Metal bindingi185ZincUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 16ATPCurated8
Nucleotide bindingi87 – 90ATPUniRule annotation4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • thymidine kinase activity Source: EcoCyc

GO - Biological processi

  • DNA biosynthetic process Source: UniProtKB-KW
  • DNA metabolic process Source: EcoCyc
  • thymidine metabolic process Source: GO_Central

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processDNA synthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:TDK-MONOMER
MetaCyc:TDK-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:tdkUniRule annotation
Ordered Locus Names:b1238, JW1226
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10994 tdk

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001749711 – 205Thymidine kinaseAdd BLAST205

Proteomic databases

PaxDbiP23331
PRIDEiP23331

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

BioGridi4259556, 225 interactors
IntActiP23331, 6 interactors
STRINGi316385.ECDH10B_1298

Structurei

3D structure databases

ProteinModelPortaliP23331
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107104 Bacteria
COG1435 LUCA
HOGENOMiHOG000076391
InParanoidiP23331
KOiK00857
OMAiKEQFGWI
PhylomeDBiP23331

Family and domain databases

HAMAPiMF_00124 Thymidine_kinase, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR001267 Thymidine_kinase
IPR020633 Thymidine_kinase_CS
PANTHERiPTHR11441 PTHR11441, 1 hit
PfamiView protein in Pfam
PF00265 TK, 1 hit
PIRSFiPIRSF035805 TK_cell, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00603 TK_CELLULAR_TYPE, 1 hit

Sequencei

Sequence statusi: Complete.

P23331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLYFYYSA MNAGKSTALL QSSYNYQERG MRTVVYTAEI DDRFGAGKVS
60 70 80 90 100
SRIGLSSPAK LFNQNSSLFD EIRAEHEQQA IHCVLVDECQ FLTRQQVYEL
110 120 130 140 150
SEVVDQLDIP VLCYGLRTDF RGELFIGSQY LLAWSDKLVE LKTICFCGRK
160 170 180 190 200
ASMVLRLDQA GRPYNEGEQV VIGGNERYVS VCRKHYKEAL QVDSLTAIQE

RHRHD
Length:205
Mass (Da):23,457
Last modified:November 1, 1991 - v1
Checksum:i533166D9EAD20E16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51523 Genomic DNA Translation: CAA35907.1
X53733 Genomic DNA Translation: CAA37765.1
X67326 Genomic DNA Translation: CAA47741.1
DQ384607 Genomic DNA Translation: ABD37701.1
U00096 Genomic DNA Translation: AAC74320.1
AP009048 Genomic DNA Translation: BAA36118.1
PIRiJS0519
RefSeqiNP_415754.1, NC_000913.3
WP_000068077.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74320; AAC74320; b1238
BAA36118; BAA36118; BAA36118
GeneIDi945834
KEGGiecj:JW1226
eco:b1238
PATRICifig|1411691.4.peg.1047

Similar proteinsi

Entry informationi

Entry nameiKITH_ECOLI
AccessioniPrimary (citable) accession number: P23331
Secondary accession number(s): Q0H0G7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 25, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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