UniProtKB - P23202 (URE2_YEAST)
Protein
Transcriptional regulator URE2
Gene
URE2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Plays an important role in nitrogen catabolite repression. Down-regulates the expression of many genes involved in nitrogen utilization by inhibiting the GATA transcriptional activators GLN3 and GAT1. Under good nitrogen conditions, binds to the phosphorylated forms of GLN3 and GAT1 and sequesters them in the cytoplasm, preventing transcription of genes expressed upon nitrogen limitation. Is also an atypical glutaredoxin without a catalytical cysteine residue. Has glutathione peroxidase and thiol:disulfide oxidoreductase activities in both native and fibrillar form. Also shows insulin disulfide reductase and dehydroascorbic acid reductase (DHAR) actvites.6 Publications
Miscellaneous
[URE3] is the prion form of URE2. [URE3] is the result of a conformational change of the cellular URE2 protein that becomes self-propagating and infectious. This conformational change generates a form of URE2 that assembles into amyloid fibrils. [URE3]-aggregates sequester soluble URE2, which then fails to retain GLN3 in the cytoplasm, resulting in GLN3 activation and consequently derepression of genes that are required for utilization of poor nirogen sources (PubMed:7909170). [URE3] can be cured by GdnHCl and by deletion of the molecular chaperone HSP104, which is required for [URE3] propagation (PubMed:11073991).2 Publications
Present with 7060 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.11.1.91 Publication
Kineticsi
kcat is 1.2 sec(-1) with bis-(2-hydroxyethyl) disulfide (HEDS) as substrate.
- KM=2.4 mM for bis-(2-hydroxyethyl) disulfide (HEDS)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 124 | Glutathione1 Publication | 1 | |
Binding sitei | 151 | Glutathione1 Publication | 1 |
GO - Molecular functioni
- glutathione peroxidase activity Source: SGD
- phosphoprotein binding Source: SGD
- transcription corepressor activity Source: InterPro
GO - Biological processi
- cytoplasmic sequestering of transcription factor Source: SGD
- glutathione metabolic process Source: InterPro
- nitrate assimilation Source: UniProtKB-KW
- protein urmylation Source: SGD
- regulation of nitrogen utilization Source: SGD
- response to aluminum ion Source: SGD
Keywordsi
Molecular function | Oxidoreductase, Prion |
Biological process | Nitrate assimilation |
Protein family/group databases
MoonProti | P23202 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:URE2 Ordered Locus Names:YNL229C ORF Names:N1165 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005173, URE2 |
VEuPathDBi | FungiDB:YNL229C |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Amyloid, CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 122 | A → S: Reduces glutaredoxin activity. 1 Publication | 1 | |
Mutagenesisi | 124 | N → A or V: Abolishes glutaredoxin activity. 1 Publication | 1 | |
Mutagenesisi | 313 | F → S: Destroys protein function. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000186013 | 2 – 354 | Transcriptional regulator URE2Add BLAST | 353 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylmethionineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | P23202 |
PaxDbi | P23202 |
PRIDEi | P23202 |
PTM databases
iPTMneti | P23202 |
Interactioni
Subunit structurei
Homodimer.
Interacts with NNK1.
2 PublicationsBinary interactionsi
Hide detailsP23202
With | #Exp. | IntAct |
---|---|---|
GLN3 [P18494] | 2 | EBI-20138,EBI-7657 |
NNK1 [P36003] | 4 | EBI-20138,EBI-9796 |
GO - Molecular functioni
- phosphoprotein binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 35609, 347 interactors |
DIPi | DIP-1308N |
IntActi | P23202, 33 interactors |
MINTi | P23202 |
STRINGi | 4932.YNL229C |
Miscellaneous databases
RNActi | P23202, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P23202 |
SMRi | P23202 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23202 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 112 – 196 | GST N-terminalAdd BLAST | 85 | |
Domaini | 205 – 354 | GST C-terminalAdd BLAST | 150 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 89 | Prion domain (PrD)Add BLAST | 88 | |
Regioni | 164 – 165 | Glutathione binding | 2 | |
Regioni | 180 – 181 | Glutathione binding | 2 |
Domaini
The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.2 Publications
Sequence similaritiesi
Belongs to the GST superfamily.Curated
Phylogenomic databases
eggNOGi | KOG0867, Eukaryota |
HOGENOMi | CLU_011226_14_1_1 |
InParanoidi | P23202 |
OMAi | KFFQNQP |
Family and domain databases
DisProti | DP00353 |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR004045, Glutathione_S-Trfase_N IPR004046, GST_C IPR036249, Thioredoxin-like_sf IPR017298, Ure2 |
Pfami | View protein in Pfam PF00043, GST_C, 1 hit PF02798, GST_N, 1 hit |
PIRSFi | PIRSF037861, Prion_URE2, 1 hit |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P23202-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMNNNGNQVS NLSNALRQVN IGNRNSNTTT DQSNINFEFS TGVNNNNNNN
60 70 80 90 100
SSSNNNNVQN NNSGRNGSQN NDNENNIKNT LEQHRQQQQA FSDMSHVEYS
110 120 130 140 150
RITKFFQEQP LEGYTLFSHR SAPNGFKVAI VLSELGFHYN TIFLDFNLGE
160 170 180 190 200
HRAPEFVSVN PNARVPALID HGMDNLSIWE SGAILLHLVN KYYKETGNPL
210 220 230 240 250
LWSDDLADQS QINAWLFFQT SGHAPMIGQA LHFRYFHSQK IASAVERYTD
260 270 280 290 300
EVRRVYGVVE MALAERREAL VMELDTENAA AYSAGTTPMS QSRFFDYPVW
310 320 330 340 350
LVGDKLTIAD LAFVPWNNVV DRIGINIKIE FPEVYKWTKH MMRRPAVIKA
LRGE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M35268 Genomic DNA Translation: AAA35201.1 AF525174 Genomic DNA Translation: AAM93167.1 AF525175 Genomic DNA Translation: AAM93168.1 AF525176 Genomic DNA Translation: AAM93169.1 AF525177 Genomic DNA Translation: AAM93170.1 AF525178 Genomic DNA Translation: AAM93171.1 AF525179 Genomic DNA Translation: AAM93172.1 AF525180 Genomic DNA Translation: AAM93173.1 AF525181 Genomic DNA Translation: AAM93174.1 AF525182 Genomic DNA Translation: AAM93175.1 AF525183 Genomic DNA Translation: AAM93176.1 AF525184 Genomic DNA Translation: AAM93177.1 AF525185 Genomic DNA Translation: AAM93178.1 AF525186 Genomic DNA Translation: AAM93179.1 AF525187 Genomic DNA Translation: AAM93180.1 AF525188 Genomic DNA Translation: AAM93181.1 AF525189 Genomic DNA Translation: AAM93182.1 AF525190 Genomic DNA Translation: AAM93183.1 AF525191 Genomic DNA Translation: AAM93184.1 AF525192 Genomic DNA Translation: AAM93185.1 Z69381 Genomic DNA Translation: CAA93369.1 Z71505 Genomic DNA Translation: CAA96134.1 BK006947 Genomic DNA Translation: DAA10329.1 |
PIRi | A39609 |
RefSeqi | NP_014170.1, NM_001183067.1 |
Genome annotation databases
EnsemblFungii | YNL229C_mRNA; YNL229C; YNL229C |
GeneIDi | 855492 |
KEGGi | sce:YNL229C |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight Mad yeast disease - Issue 47 of June 2004 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M35268 Genomic DNA Translation: AAA35201.1 AF525174 Genomic DNA Translation: AAM93167.1 AF525175 Genomic DNA Translation: AAM93168.1 AF525176 Genomic DNA Translation: AAM93169.1 AF525177 Genomic DNA Translation: AAM93170.1 AF525178 Genomic DNA Translation: AAM93171.1 AF525179 Genomic DNA Translation: AAM93172.1 AF525180 Genomic DNA Translation: AAM93173.1 AF525181 Genomic DNA Translation: AAM93174.1 AF525182 Genomic DNA Translation: AAM93175.1 AF525183 Genomic DNA Translation: AAM93176.1 AF525184 Genomic DNA Translation: AAM93177.1 AF525185 Genomic DNA Translation: AAM93178.1 AF525186 Genomic DNA Translation: AAM93179.1 AF525187 Genomic DNA Translation: AAM93180.1 AF525188 Genomic DNA Translation: AAM93181.1 AF525189 Genomic DNA Translation: AAM93182.1 AF525190 Genomic DNA Translation: AAM93183.1 AF525191 Genomic DNA Translation: AAM93184.1 AF525192 Genomic DNA Translation: AAM93185.1 Z69381 Genomic DNA Translation: CAA93369.1 Z71505 Genomic DNA Translation: CAA96134.1 BK006947 Genomic DNA Translation: DAA10329.1 |
PIRi | A39609 |
RefSeqi | NP_014170.1, NM_001183067.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G6W | X-ray | 2.50 | A/B/C/D | 94-354 | [»] | |
1G6Y | X-ray | 2.80 | A/B | 94-354 | [»] | |
1HQO | X-ray | 2.30 | A/B | 97-354 | [»] | |
1JZR | X-ray | 2.90 | A/B/C/D | 95-354 | [»] | |
1K0A | X-ray | 2.50 | A/B | 95-354 | [»] | |
1K0B | X-ray | 2.50 | A/B/C/D | 95-354 | [»] | |
1K0C | X-ray | 2.50 | A/B/C/D | 95-354 | [»] | |
1K0D | X-ray | 2.20 | A/B/C/D | 95-354 | [»] | |
BMRBi | P23202 | |||||
SMRi | P23202 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35609, 347 interactors |
DIPi | DIP-1308N |
IntActi | P23202, 33 interactors |
MINTi | P23202 |
STRINGi | 4932.YNL229C |
Protein family/group databases
MoonProti | P23202 |
PTM databases
iPTMneti | P23202 |
Proteomic databases
MaxQBi | P23202 |
PaxDbi | P23202 |
PRIDEi | P23202 |
Genome annotation databases
EnsemblFungii | YNL229C_mRNA; YNL229C; YNL229C |
GeneIDi | 855492 |
KEGGi | sce:YNL229C |
Organism-specific databases
SGDi | S000005173, URE2 |
VEuPathDBi | FungiDB:YNL229C |
Phylogenomic databases
eggNOGi | KOG0867, Eukaryota |
HOGENOMi | CLU_011226_14_1_1 |
InParanoidi | P23202 |
OMAi | KFFQNQP |
Miscellaneous databases
EvolutionaryTracei | P23202 |
PROi | PR:P23202 |
RNActi | P23202, protein |
Family and domain databases
DisProti | DP00353 |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR004045, Glutathione_S-Trfase_N IPR004046, GST_C IPR036249, Thioredoxin-like_sf IPR017298, Ure2 |
Pfami | View protein in Pfam PF00043, GST_C, 1 hit PF02798, GST_N, 1 hit |
PIRSFi | PIRSF037861, Prion_URE2, 1 hit |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | URE2_YEAST | |
Accessioni | P23202Primary (citable) accession number: P23202 Secondary accession number(s): D6W0W3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | April 7, 2021 | |
This is version 198 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries