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UniProtKB - P23192 (MTM2_MORBO)
Protein
Type II methyltransferase M1.MboII
Gene
mboIIM
Organism
Moraxella bovis
Status
Functioni
A beta subtype methylase that recognizes the double-stranded sequence 5'-GAAGA-3', methylates A-5 on the top strand, and protects the DNA from cleavage by the MboII endonuclease. It is not known if the cytosine of the complementary sequence TCTTC is also methylated by this enzyme.
1 PublicationCatalytic activityi
- a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H+ + S-adenosyl-L-homocysteineEC:2.1.1.72
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | S-adenosyl-L-methionine; via amide nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 30 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 197 | S-adenosyl-L-methionine; via amide nitrogenCombined sources1 Publication | 1 |
GO - Molecular functioni
- DNA binding Source: InterPro
- N-methyltransferase activity Source: InterPro
- site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Methyltransferase, Transferase |
Biological process | Restriction system |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.72, 3416 |
Protein family/group databases
REBASEi | 3441, M1.MboII |
Names & Taxonomyi
Protein namesi | Recommended name: Type II methyltransferase M1.MboII1 Publication (EC:2.1.1.72)Short name: M1.MboII1 Publication Alternative name(s): Adenine-specific methyltransferase MboII DNA MTase MboIIA Modification methylase MboII Short name: M.MboII1 Publication |
Gene namesi | Name:mboIIM1 Publication |
Organismi | Moraxella bovis |
Taxonomic identifieri | 476 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Moraxellales › Moraxellaceae › Moraxella |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087967 | 1 – 260 | Type II methyltransferase M1.MboIIAdd BLAST | 260 |
Interactioni
Subunit structurei
At low concentration exists as a monomer and homodimer (PubMed:12954781). Probably binds to DNA as a monomer (Probable).
1 Publication1 PublicationProtein-protein interaction databases
STRINGi | 476.B0182_06905 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P23192 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P23192 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 223 – 225 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 3 | |
Regioni | 241 – 242 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 2 |
Sequence similaritiesi
Belongs to the N(4)/N(6)-methyltransferase family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR002941, DNA_methylase_N4/N6 IPR002052, DNA_methylase_N6_adenine_CS IPR001091, RM_Methylase IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF01555, N6_N4_Mtase, 1 hit |
PRINTSi | PR00508, S21N4MTFRASE |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P23192-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLEINKIHQM NCFDFLDQVE NKSVQLAVID PPYNLSKADW DSFDSHNEFL
60 70 80 90 100
PFTYRWIDKV LDKLDKDGSL YIFNTPFNCA FICQYLVSKG MIFQNWITWD
110 120 130 140 150
KRDGMGSAKR GFSTGQETIL FFSKSKNHTF NYDEVRVPYE STDRIKHASE
160 170 180 190 200
KGILKNGKRW FPNPNGRLCG EVWHFSSQRH KEKVNGKTVK LTHITPKPRD
210 220 230 240 250
LIERIIRASS NPNDLVLDCF MGSGTTAIVA KKLGRNFIGC DMNAEYVNQA
260
NFVLNQLEIN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56977 Genomic DNA Translation: CAA40297.1 |
PIRi | S26835 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56977 Genomic DNA Translation: CAA40297.1 |
PIRi | S26835 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G60 | X-ray | 1.74 | A/B | 1-260 | [»] | |
SMRi | P23192 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 476.B0182_06905 |
Protein family/group databases
REBASEi | 3441, M1.MboII |
Enzyme and pathway databases
BRENDAi | 2.1.1.72, 3416 |
Miscellaneous databases
EvolutionaryTracei | P23192 |
PROi | PR:P23192 |
Family and domain databases
InterProi | View protein in InterPro IPR002941, DNA_methylase_N4/N6 IPR002052, DNA_methylase_N6_adenine_CS IPR001091, RM_Methylase IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF01555, N6_N4_Mtase, 1 hit |
PRINTSi | PR00508, S21N4MTFRASE |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MTM2_MORBO | |
Accessioni | P23192Primary (citable) accession number: P23192 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1991 |
Last sequence update: | November 1, 1991 | |
Last modified: | February 23, 2022 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families