Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable mediator of RNA polymerase II transcription subunit 37c

Gene

MED37D

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371453 Regulation of HSF1-mediated heat shock response
R-ATH-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-ATH-3371571 HSF1-dependent transactivation
R-ATH-6798695 Neutrophil degranulation
R-ATH-72163 mRNA Splicing - Major Pathway
R-ATH-8876725 Protein methylation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mediator of RNA polymerase II transcription subunit 37c
Alternative name(s):
Heat shock 70 kDa protein 2
Heat shock cognate 70 kDa protein 2
Heat shock cognate protein 70-2
Short name:
AtHsc70-2
Heat shock protein 70-2
Short name:
AtHsp70-2
Gene namesi
Name:MED37D
Synonyms:HSC70-2, HSC70-G8, HSP70-2, MED37_3
Ordered Locus Names:At5g02490
ORF Names:T22P11.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G02490
TAIRilocus:2181818 AT5G02490

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000783451 – 653Probable mediator of RNA polymerase II transcription subunit 37cAdd BLAST653

Proteomic databases

PaxDbiP22954
PRIDEiP22954

PTM databases

iPTMnetiP22954

Expressioni

Developmental stagei

Down-regulated during seed maturation.1 Publication

Inductioni

By heat shock and by cold. Up-regulated by virus infection and by pathogen attack (P.syringae).3 Publications

Gene expression databases

ExpressionAtlasiP22954 baseline and differential
GenevisibleiP22954 AT

Interactioni

Subunit structurei

Component of the Mediator complex.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi17132, 13 interactors
IntActiP22954, 2 interactors
STRINGi3702.AT5G02490.1

Structurei

3D structure databases

ProteinModelPortaliP22954
SMRiP22954
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0101 Eukaryota
COG0443 LUCA
HOGENOMiHOG000228135
InParanoidiP22954
KOiK03283
OMAiXHESERA
OrthoDBiEOG093604KP
PhylomeDBiP22954

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

P22954-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGKGEGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS
60 70 80 90 100
ERLIGDAAKN QVAMNPVNTV FDAKRLIGRR FSDASVQSDR QLWPFTIISG
110 120 130 140 150
TAEKPMIVVE YKGEEKQFAA EEISSMVLIK MREIAEAFLG TTVKNAVVTV
160 170 180 190 200
PAYFNDSQRQ ATKDAGVIAG LNVLRIINEP TAAAIAYGLD KKATSVGEKN
210 220 230 240 250
VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE
260 270 280 290 300
FKRKNKQDIT GQPRALRRLR TACERAKRTL SSTAQTTIEI DSLYGGADFY
310 320 330 340 350
SPITRARFEE MNMDLFRKCM EPVEKCLRDA KMDKSTVHEI VLVGGSTRIP
360 370 380 390 400
KVQQLLQDFF NGKELCKSIN PDEAVAYGAA VQAAILSGEG NEKVQDLLLL
410 420 430 440 450
DVTPLSLGLE TAGGVMTTLI QRNTTIPTKK EQVFSTYSDN QPGVLIQVFE
460 470 480 490 500
GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT
510 520 530 540 550
TGKKNKITIT NDKGRLSKED IEKMVQEAEK YKSEDEEHKK KVEAKNALEN
560 570 580 590 600
YAYNMRNTIR DEKIGEKLPA ADKKKVEDSI EEAIQWLDGN QLGEADEFED
610 620 630 640 650
KMKELESVCN PIIAKMYQGG AGGEAGGPGA SGMDEDEAPP ASGGAGPKIE

EVD
Length:653
Mass (Da):71,387
Last modified:December 1, 2000 - v2
Checksum:iEDEC254DB06D9966
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6E → K in CAA54420 (Ref. 6) Curated1
Sequence conflicti100 – 102GTA → ELQ in AAA32820 (PubMed:16666375).Curated3
Sequence conflicti614A → G in BAD94888 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL162971 Genomic DNA Translation: CAB85986.1
CP002688 Genomic DNA Translation: AED90479.1
AY093152 mRNA Translation: AAM13151.1
BT008411 mRNA Translation: AAP37770.1
AK222068 mRNA Translation: BAD94888.1
AK227090 mRNA Translation: BAE99142.1
AH001335 Genomic DNA Translation: AAA32820.1
X77199 Genomic DNA Translation: CAA54420.1
Y08892 mRNA Translation: CAA70105.1
PIRiJA0170
T48270
RefSeqiNP_195869.1, NM_120327.5
UniGeneiAt.5363
At.74798

Genome annotation databases

EnsemblPlantsiAT5G02490.1; AT5G02490.1; AT5G02490
GeneIDi831856
GrameneiAT5G02490.1; AT5G02490.1; AT5G02490
KEGGiath:AT5G02490

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL162971 Genomic DNA Translation: CAB85986.1
CP002688 Genomic DNA Translation: AED90479.1
AY093152 mRNA Translation: AAM13151.1
BT008411 mRNA Translation: AAP37770.1
AK222068 mRNA Translation: BAD94888.1
AK227090 mRNA Translation: BAE99142.1
AH001335 Genomic DNA Translation: AAA32820.1
X77199 Genomic DNA Translation: CAA54420.1
Y08892 mRNA Translation: CAA70105.1
PIRiJA0170
T48270
RefSeqiNP_195869.1, NM_120327.5
UniGeneiAt.5363
At.74798

3D structure databases

ProteinModelPortaliP22954
SMRiP22954
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17132, 13 interactors
IntActiP22954, 2 interactors
STRINGi3702.AT5G02490.1

PTM databases

iPTMnetiP22954

Proteomic databases

PaxDbiP22954
PRIDEiP22954

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G02490.1; AT5G02490.1; AT5G02490
GeneIDi831856
GrameneiAT5G02490.1; AT5G02490.1; AT5G02490
KEGGiath:AT5G02490

Organism-specific databases

AraportiAT5G02490
TAIRilocus:2181818 AT5G02490

Phylogenomic databases

eggNOGiKOG0101 Eukaryota
COG0443 LUCA
HOGENOMiHOG000228135
InParanoidiP22954
KOiK03283
OMAiXHESERA
OrthoDBiEOG093604KP
PhylomeDBiP22954

Enzyme and pathway databases

ReactomeiR-ATH-3371453 Regulation of HSF1-mediated heat shock response
R-ATH-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-ATH-3371571 HSF1-dependent transactivation
R-ATH-6798695 Neutrophil degranulation
R-ATH-72163 mRNA Splicing - Major Pathway
R-ATH-8876725 Protein methylation

Miscellaneous databases

PROiPR:P22954

Gene expression databases

ExpressionAtlasiP22954 baseline and differential
GenevisibleiP22954 AT

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMD37D_ARATH
AccessioniPrimary (citable) accession number: P22954
Secondary accession number(s): O04293
, Q0WUQ6, Q56WH2, Q9LZ53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 1, 2000
Last modified: November 7, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again