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Protein

DNA-(apurinic or apyrimidinic site) lyase 1

Gene

APN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. APN1 accounts for > 97% of both apurinic/ apyrimidinic (AP) lyase and DNA 3'-repair diesterase activities.

Miscellaneous

Present with 7250 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactori

Zn2+Note: Binds 3 Zn2+ ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi83Zinc 1By similarity1
Metal bindingi123Zinc 1By similarity1
Metal bindingi158Zinc 1By similarity1
Metal bindingi158Zinc 2By similarity1
Metal bindingi192Zinc 2By similarity1
Metal bindingi195Zinc 3By similarity1
Metal bindingi229Zinc 2By similarity1
Metal bindingi242Zinc 3By similarity1
Metal bindingi244Zinc 3By similarity1
Metal bindingi274Zinc 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31899-MONOMER
BRENDAi4.2.99.18 984

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-(apurinic or apyrimidinic site) lyase 1 (EC:4.2.99.18)
Alternative name(s):
Apurinic-apyrimidinic endonuclease 1
Short name:
AP endonuclease 1
Gene namesi
Name:APN1
Ordered Locus Names:YKL114C
ORF Names:YKL513
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL114C
SGDiS000001597 APN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001908982 – 367DNA-(apurinic or apyrimidinic site) lyase 1Add BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei356PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP22936
PaxDbiP22936
PRIDEiP22936

PTM databases

iPTMnetiP22936

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi34020, 107 interactors
DIPiDIP-4106N
IntActiP22936, 2 interactors
MINTiP22936
STRINGi4932.YKL114C

Structurei

3D structure databases

ProteinModelPortaliP22936
SMRiP22936
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013698
HOGENOMiHOG000224893
InParanoidiP22936
KOiK10771
OMAiHPGSHLK
OrthoDBiEOG092C2ZL3

Family and domain databases

CDDicd00019 AP2Ec, 1 hit
HAMAPiMF_00152 Nfo, 1 hit
InterProiView protein in InterPro
IPR001719 AP_endonuc_2
IPR018246 AP_endonuc_F2_Zn_BS
IPR036237 Xyl_isomerase-like_sf
IPR013022 Xyl_isomerase-like_TIM-brl
PANTHERiPTHR21445 PTHR21445, 1 hit
PfamiView protein in Pfam
PF01261 AP_endonuc_2, 1 hit
SMARTiView protein in SMART
SM00518 AP2Ec, 1 hit
SUPFAMiSSF51658 SSF51658, 1 hit
TIGRFAMsiTIGR00587 nfo, 1 hit
PROSITEiView protein in PROSITE
PS00729 AP_NUCLEASE_F2_1, 1 hit
PS00730 AP_NUCLEASE_F2_2, 1 hit
PS00731 AP_NUCLEASE_F2_3, 1 hit
PS51432 AP_NUCLEASE_F2_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22936-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPSTPSFVRS AVSKYKFGAH MSGAGGISNS VTNAFNTGCN SFAMFLKSPR
60 70 80 90 100
KWVSPQYTQE EIDKFKKNCA TYNYNPLTDV LPHGQYFINL ANPDREKAEK
110 120 130 140 150
SYESFMDDLN RCEQLGIGLY NLHPGSTLKG DHQLQLKQLA SYLNKAIKET
160 170 180 190 200
KFVKIVLENM AGTGNLVGSS LVDLKEVIGM IEDKSRIGVC IDTCHTFAAG
210 220 230 240 250
YDISTTETFN NFWKEFNDVI GFKYLSAVHL NDSKAPLGAN RDLHERLGQG
260 270 280 290 300
YLGIDVFRMI AHSEYLQGIP IVLETPYEND EGYGNEIKLM EWLESKSESE
310 320 330 340 350
LLEDKEYKEK NDTLQKLGAK SRKEQLDKFE VKQKKRAGGT KRKKATAEPS
360
DNDILSQMTK KRKTKKE
Length:367
Mass (Da):41,439
Last modified:January 23, 2007 - v4
Checksum:i76C14935B760A6D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239A → S in AAA34429 (PubMed:1693433).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33667 Genomic DNA Translation: AAA34429.1
S93804 Genomic DNA Translation: AAB22003.1
Z28114 Genomic DNA Translation: CAA81954.1
BK006944 Genomic DNA Translation: DAA09044.1
PIRiS29871
RefSeqiNP_012808.1, NM_001179680.1

Genome annotation databases

EnsemblFungiiYKL114C; YKL114C; YKL114C
GeneIDi853746
KEGGisce:YKL114C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33667 Genomic DNA Translation: AAA34429.1
S93804 Genomic DNA Translation: AAB22003.1
Z28114 Genomic DNA Translation: CAA81954.1
BK006944 Genomic DNA Translation: DAA09044.1
PIRiS29871
RefSeqiNP_012808.1, NM_001179680.1

3D structure databases

ProteinModelPortaliP22936
SMRiP22936
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34020, 107 interactors
DIPiDIP-4106N
IntActiP22936, 2 interactors
MINTiP22936
STRINGi4932.YKL114C

PTM databases

iPTMnetiP22936

Proteomic databases

MaxQBiP22936
PaxDbiP22936
PRIDEiP22936

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL114C; YKL114C; YKL114C
GeneIDi853746
KEGGisce:YKL114C

Organism-specific databases

EuPathDBiFungiDB:YKL114C
SGDiS000001597 APN1

Phylogenomic databases

GeneTreeiENSGT00390000013698
HOGENOMiHOG000224893
InParanoidiP22936
KOiK10771
OMAiHPGSHLK
OrthoDBiEOG092C2ZL3

Enzyme and pathway databases

BioCyciYEAST:G3O-31899-MONOMER
BRENDAi4.2.99.18 984

Miscellaneous databases

PROiPR:P22936

Family and domain databases

CDDicd00019 AP2Ec, 1 hit
HAMAPiMF_00152 Nfo, 1 hit
InterProiView protein in InterPro
IPR001719 AP_endonuc_2
IPR018246 AP_endonuc_F2_Zn_BS
IPR036237 Xyl_isomerase-like_sf
IPR013022 Xyl_isomerase-like_TIM-brl
PANTHERiPTHR21445 PTHR21445, 1 hit
PfamiView protein in Pfam
PF01261 AP_endonuc_2, 1 hit
SMARTiView protein in SMART
SM00518 AP2Ec, 1 hit
SUPFAMiSSF51658 SSF51658, 1 hit
TIGRFAMsiTIGR00587 nfo, 1 hit
PROSITEiView protein in PROSITE
PS00729 AP_NUCLEASE_F2_1, 1 hit
PS00730 AP_NUCLEASE_F2_2, 1 hit
PS00731 AP_NUCLEASE_F2_3, 1 hit
PS51432 AP_NUCLEASE_F2_4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAPN1_YEAST
AccessioniPrimary (citable) accession number: P22936
Secondary accession number(s): D6VXH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 165 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names
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Main funding by: National Institutes of Health

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