UniProtKB - P22892 (AP1G1_MOUSE)
AP-1 complex subunit gamma-1
Ap1g1
Functioni
GO - Molecular functioni
- cargo adaptor activity Source: GO_Central
- clathrin adaptor activity Source: GO_Central
- collagen binding Source: MGI
- GTP-dependent protein binding Source: MGI
- kinesin binding Source: MGI
- protein C-terminus binding Source: MGI
- Rab GTPase binding Source: MGI
GO - Biological processi
- endosome to melanosome transport Source: MGI
- Golgi to lysosome transport Source: MGI
- Golgi to vacuole transport Source: GO_Central
- intracellular protein transport Source: MGI
- microtubule cytoskeleton organization Source: MGI
- positive regulation of natural killer cell degranulation Source: MGI
- positive regulation of natural killer cell mediated cytotoxicity Source: MGI
- receptor-mediated endocytosis Source: GO_Central
- vesicle-mediated transport Source: MGI
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-2132295, MHC class II antigen presentation R-MMU-432720, Lysosome Vesicle Biogenesis R-MMU-432722, Golgi Associated Vesicle Biogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: AP-1 complex subunit gamma-1Alternative name(s): Adaptor protein complex AP-1 subunit gamma-1 Adaptor-related protein complex 1 subunit gamma-1 Clathrin assembly protein complex 1 gamma-1 large chain Gamma-adaptin Gamma1-adaptin Golgi adaptor HA1/AP1 adaptin subunit gamma-1 |
Gene namesi | Name:Ap1g1 Synonyms:Adtg, Clapg1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101919, Ap1g1 |
Subcellular locationi
Golgi apparatus
Other locations
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Cytoskeleton
- microtubule cytoskeleton Source: MGI
Cytosol
- cytosol Source: MGI
Endosome
- recycling endosome Source: MGI
Golgi apparatus
- AP-1 adaptor complex Source: GO_Central
- Golgi apparatus Source: MGI
- trans-Golgi network Source: MGI
Other locations
- clathrin-coated vesicle Source: MGI
- intracellular membrane-bounded organelle Source: MGI
- perinuclear region of cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasmic vesicle, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 753 | A → D: Strongly reduces interaction with EPS15 and SYNRG. 1 Publication | 1 | |
Mutagenesisi | 762 | L → E: Strongly reduces interaction with EPS15 and SYNRG. 1 Publication | 1 | |
Mutagenesisi | 765 | P → N: Reduces interaction with EPS15 and SYNRG. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000193759 | 2 – 822 | AP-1 complex subunit gamma-1Add BLAST | 821 |
Proteomic databases
EPDi | P22892 |
jPOSTi | P22892 |
MaxQBi | P22892 |
PaxDbi | P22892 |
PeptideAtlasi | P22892 |
PRIDEi | P22892 |
PTM databases
iPTMneti | P22892 |
PhosphoSitePlusi | P22892 |
SwissPalmi | P22892 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000031731, Expressed in forelimb bud and 298 other tissues |
ExpressionAtlasi | P22892, baseline and differential |
Genevisiblei | P22892, MM |
Interactioni
Subunit structurei
Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3) (By similarity). Binds RABEP1 (By similarity). Binds EPS15 and SYNRG.
Interacts (via GAE domain) with AP1AR (via coiled-coil domain) (By similarity).
Interacts with CLN3 (via dileucine motif); this interaction facilitates lysosomal targeting (By similarity).
By similarityBinary interactionsi
Hide detailsP22892
With | #Exp. | IntAct |
---|---|---|
Ap1m1 [P35585] | 6 | EBI-1040262,EBI-1040251 |
CLINT1 [Q14677] from Homo sapiens. | 10 | EBI-1040262,EBI-1171113 |
GO - Molecular functioni
- clathrin adaptor activity Source: GO_Central
- GTP-dependent protein binding Source: MGI
- kinesin binding Source: MGI
- protein C-terminus binding Source: MGI
- Rab GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198123, 22 interactors |
ComplexPortali | CPX-5141, Ubiquitous AP-1 Adaptor complex, sigma1a variant CPX-5142, Ubiquitous AP-1 Adaptor complex, sigma1b variant CPX-5143, Ubiquitous AP-1 Adaptor complex, sigma1c variant CPX-5144, Endothelial AP-1 Adaptor complex, sigma1a variant |
CORUMi | P22892 |
IntActi | P22892, 18 interactors |
MINTi | P22892 |
STRINGi | 10090.ENSMUSP00000090844 |
Miscellaneous databases
RNActi | P22892, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P22892 |
SMRi | P22892 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P22892 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 702 – 817 | GAEPROSITE-ProRule annotationAdd BLAST | 116 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1062, Eukaryota |
GeneTreei | ENSGT00950000182838 |
HOGENOMi | CLU_003824_0_0_1 |
InParanoidi | P22892 |
PhylomeDBi | P22892 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR017107, AP1_complex_gsu IPR011989, ARM-like IPR016024, ARM-type_fold IPR002553, Clathrin/coatomer_adapt-like_N IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR008153, GAE_dom |
Pfami | View protein in Pfam PF01602, Adaptin_N, 1 hit PF02883, Alpha_adaptinC2, 1 hit |
PIRSFi | PIRSF037094, AP1_complex_gamma, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49348, SSF49348, 1 hit |
PROSITEi | View protein in PROSITE PS50180, GAE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV
60 70 80 90 100
AKLLYMHMLG YPAHFGQLEC LKLIASQKFT DKRIGYLGAM LLLDERQDVH
110 120 130 140 150
LLMTNCIKND LNHSTQFVQG LALCTLGCMG SSEMCRDLAG EVEKLLKTSN
160 170 180 190 200
SYLRKKAALC AVHVIRKVPE LMEMFLPATK NLLNEKNHGV LHTSVVLLTE
210 220 230 240 250
MCERSPDMLA HFRKLVPQLV RILKNLIMSG YSPEHDVSGI SDPFLQVRIL
260 270 280 290 300
RLLRILGRND DDSSEAMNDI LAQVATNTET SKNVGNAILY ETVLTIMDIK
310 320 330 340 350
SESGLRVLAI NILGRFLLNN DKNIRYVALT SLLKTVQTDH NAVQRHRSTI
360 370 380 390 400
VDCLKDLDVS IKRRAMELSF ALVNGNNIRG MMKELLYFLD SCEPEFKADC
410 420 430 440 450
ASGIFLAAEK YAPSKRWHID TIMRVLTTAG SYVRDDAVPN LIQLITNSVE
460 470 480 490 500
MHAYTVQRLY KAILGDYSQQ PLVQVAAWCI GEYGDLLVSG QCEEEEPIQV
510 520 530 540 550
TEDEVLDILE SVLISNMSTS VTRGYALTAI MKLSTRFTCT VNRIKKVVSI
560 570 580 590 600
YGSSIDVELQ QRAVEYNALF KKYDHMRSAL LERMPVMEKV TTNGPSEIVQ
610 620 630 640 650
TNGETEPAPL ETKPPPSGPQ PTSQANDLLD LLGGNDITPV IPTAPTSKPA
660 670 680 690 700
SAGGELLDLL GDITLTGAPA AAPTPASVPQ ISQPPFLLDG LSSQPLFNDI
710 720 730 740 750
APGIPSITAY SKNGLKIEFT FERSNTNPSV TVITIQASNS TELDMTDFVF
760 770 780 790 800
QAAVPKTFQL QLLSPSSSVV PAFNTGTITQ VIKVLNPQKQ QLRMRIKLTY
810 820
NHKGSAMQDL AEVNNFPPQS WQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ8CBB7 | Q8CBB7_MOUSE | AP-1 complex subunit gamma | Ap1g1 | 825 | Annotation score: | ||
Q3TDN1 | Q3TDN1_MOUSE | AP-1 complex subunit gamma-1 | Ap1g1 | 49 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54424 mRNA Translation: CAA38296.1 |
CCDSi | CCDS80929.1 |
PIRi | A36680 |
RefSeqi | NP_001288140.1, NM_001301211.1 |
Genome annotation databases
Ensembli | ENSMUST00000034171; ENSMUSP00000034171; ENSMUSG00000031731 |
GeneIDi | 11765 |
KEGGi | mmu:11765 |
UCSCi | uc012gkl.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54424 mRNA Translation: CAA38296.1 |
CCDSi | CCDS80929.1 |
PIRi | A36680 |
RefSeqi | NP_001288140.1, NM_001301211.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GYU | X-ray | 1.81 | A | 704-822 | [»] | |
1GYV | X-ray | 1.71 | A | 704-822 | [»] | |
1GYW | X-ray | 2.40 | A/B | 695-822 | [»] | |
1W63 | X-ray | 4.00 | A/C/E/G/I/K | 1-613 | [»] | |
2A7B | X-ray | 1.65 | A | 704-822 | [»] | |
3ZY7 | X-ray | 1.09 | A/B | 704-822 | [»] | |
4HMY | X-ray | 7.00 | A | 1-595 | [»] | |
4P6Z | X-ray | 3.00 | G | 1-613 | [»] | |
6CM9 | electron microscopy | 3.73 | G | 1-595 | [»] | |
6CRI | electron microscopy | 6.80 | G/Q/R | 4-588 | [»] | |
6D83 | electron microscopy | 4.27 | G | 1-595 | [»] | |
6D84 | electron microscopy | 6.72 | G/K | 1-595 | [»] | |
6DFF | electron microscopy | 3.90 | G | 1-595 | [»] | |
BMRBi | P22892 | |||||
SMRi | P22892 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198123, 22 interactors |
ComplexPortali | CPX-5141, Ubiquitous AP-1 Adaptor complex, sigma1a variant CPX-5142, Ubiquitous AP-1 Adaptor complex, sigma1b variant CPX-5143, Ubiquitous AP-1 Adaptor complex, sigma1c variant CPX-5144, Endothelial AP-1 Adaptor complex, sigma1a variant |
CORUMi | P22892 |
IntActi | P22892, 18 interactors |
MINTi | P22892 |
STRINGi | 10090.ENSMUSP00000090844 |
PTM databases
iPTMneti | P22892 |
PhosphoSitePlusi | P22892 |
SwissPalmi | P22892 |
Proteomic databases
EPDi | P22892 |
jPOSTi | P22892 |
MaxQBi | P22892 |
PaxDbi | P22892 |
PeptideAtlasi | P22892 |
PRIDEi | P22892 |
Protocols and materials databases
Antibodypediai | 3960, 236 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034171; ENSMUSP00000034171; ENSMUSG00000031731 |
GeneIDi | 11765 |
KEGGi | mmu:11765 |
UCSCi | uc012gkl.3, mouse |
Organism-specific databases
CTDi | 164 |
MGIi | MGI:101919, Ap1g1 |
Phylogenomic databases
eggNOGi | KOG1062, Eukaryota |
GeneTreei | ENSGT00950000182838 |
HOGENOMi | CLU_003824_0_0_1 |
InParanoidi | P22892 |
PhylomeDBi | P22892 |
Enzyme and pathway databases
Reactomei | R-MMU-2132295, MHC class II antigen presentation R-MMU-432720, Lysosome Vesicle Biogenesis R-MMU-432722, Golgi Associated Vesicle Biogenesis |
Miscellaneous databases
BioGRID-ORCSi | 11765, 3 hits in 12 CRISPR screens |
ChiTaRSi | Ap1g1, mouse |
EvolutionaryTracei | P22892 |
PROi | PR:P22892 |
RNActi | P22892, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031731, Expressed in forelimb bud and 298 other tissues |
ExpressionAtlasi | P22892, baseline and differential |
Genevisiblei | P22892, MM |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR017107, AP1_complex_gsu IPR011989, ARM-like IPR016024, ARM-type_fold IPR002553, Clathrin/coatomer_adapt-like_N IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR008153, GAE_dom |
Pfami | View protein in Pfam PF01602, Adaptin_N, 1 hit PF02883, Alpha_adaptinC2, 1 hit |
PIRSFi | PIRSF037094, AP1_complex_gamma, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49348, SSF49348, 1 hit |
PROSITEi | View protein in PROSITE PS50180, GAE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AP1G1_MOUSE | |
Accessioni | P22892Primary (citable) accession number: P22892 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 180 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families