UniProtKB - P22772 (MTB3_ANEAE)
Protein
Modification methylase BanIII
Gene
banIIIM
Organism
Aneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
Status
Functioni
This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.
Catalytic activityi
- a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H+ + S-adenosyl-L-homocysteineEC:2.1.1.72
GO - Molecular functioni
- DNA binding Source: InterPro
- N-methyltransferase activity Source: InterPro
- site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Restriction system |
Ligand | S-adenosyl-L-methionine |
Protein family/group databases
REBASEi | 3296, M.BanIII |
Names & Taxonomyi
Protein namesi | Recommended name: Modification methylase BanIII (EC:2.1.1.72)Short name: M.BanIII Alternative name(s): Adenine-specific methyltransferase BanIII |
Gene namesi | Name:banIIIM |
Organismi | Aneurinibacillus aneurinilyticus (Bacillus aneurinolyticus) |
Taxonomic identifieri | 1391 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Aneurinibacillus group › Aneurinibacillus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087943 | 1 – 580 | Modification methylase BanIIIAdd BLAST | 580 |
Family & Domainsi
Sequence similaritiesi
Belongs to the N(4)/N(6)-methyltransferase family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR003356, DNA_methylase_A-5 IPR002052, DNA_methylase_N6_adenine_CS IPR029063, SAM-dependent_MTases IPR025931, TaqI_C |
Pfami | View protein in Pfam PF02384, N6_Mtase, 1 hit PF12950, TaqI_C, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P22772-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELTIEEMLI KQKETGAHYT PTDLGDIIAK RLINELKKSG ISGTKKIRGL
60 70 80 90 100
DPSCGDGELL LSLNRIAKFN NIDNIELIGI DEDKEAIKEA DFRLNEMGIN
110 120 130 140 150
DAKLTAGDFL DMVDLEGNLS LFDDDLSKIE PVDLIIANPP YVRTQVLGAD
160 170 180 190 200
RAQKLAKLFN LKGRVDLYHA FLVAMTLQLK PGGLIGVITS NKYLANSSGE
210 220 230 240 250
SIRQFLAENY DIIEIMDLGD TKLFSAAVLQ AIFFGRKKVN KGIRQTAPAN
260 270 280 290 300
FYKIYEETDP SKTEVSIKFE TLFGLLESSN TGVFNVDEKF YSVSCGKLIV
310 320 330 340 350
PDSFKEPWVM ATDEEYNWIT NINNNSYCTI QDLCDVKVGI KTTADKVFIK
360 370 380 390 400
STWEELPDEI KPEVEVLKLL ISTDHASKWR PLERIPNQKI LYTHENVNGK
410 420 430 440 450
KKAIDFTQYP HALAYLETHR ETLEGRKYVI KAKRNWYEIW VPQNPDHWAL
460 470 480 490 500
PKIVFPDISP EPKFFYEDEG CCIDGNCYWI IPKEENNNDI LFLILGISNT
510 520 530 540 550
KYMTNYHDIA FNNKLYPGRR RYLTQYVSKY PLPNPEANYS QEIIDLVREL
560 570 580
VFQNPNDERK IEIENQIENL TALAFGVERL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83417 Genomic DNA Translation: CAA58443.1 Sequence problems. |
PIRi | JH0224 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83417 Genomic DNA Translation: CAA58443.1 Sequence problems. |
PIRi | JH0224 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein family/group databases
REBASEi | 3296, M.BanIII |
Miscellaneous databases
PROi | PR:P22772 |
Family and domain databases
InterProi | View protein in InterPro IPR003356, DNA_methylase_A-5 IPR002052, DNA_methylase_N6_adenine_CS IPR029063, SAM-dependent_MTases IPR025931, TaqI_C |
Pfami | View protein in Pfam PF02384, N6_Mtase, 1 hit PF12950, TaqI_C, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MTB3_ANEAE | |
Accessioni | P22772Primary (citable) accession number: P22772 Secondary accession number(s): Q44656 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | August 1, 1991 | |
Last modified: | December 11, 2019 | |
This is version 73 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- SIMILARITY comments
Index of protein domains and families - Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries