UniProtKB - P22759 (BFR_AZOVI)
Protein
Bacterioferritin
Gene
bfr
Organism
Azotobacter vinelandii
Status
Functioni
Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex.By similarity
Catalytic activityi
- EC:1.16.3.1
Cofactori
Protein has several cofactor binding sites:- heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.By similarity
- Fe cationBy similarityNote: Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 18 | Iron 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 51 | Iron 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 51 | Iron 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 52 | Iron (heme axial ligand); shared with dimeric partnerPROSITE-ProRule annotation | 1 | |
Metal bindingi | 54 | Iron 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 94 | Iron 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 127 | Iron 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 127 | Iron 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 130 | Iron 2PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ferric iron binding Source: InterPro
- ferroxidase activity Source: UniProtKB-EC
GO - Biological processi
- cellular iron ion homeostasis Source: UniProtKB-KW
- iron ion transport Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Iron storage |
Ligand | Heme, Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Bacterioferritin (EC:1.16.3.1)Short name: BFR Alternative name(s): Cytochrome b-557.5 |
Gene namesi | Name:bfr |
Organismi | Azotobacter vinelandii |
Taxonomic identifieri | 354 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192588 | 1 – 156 | BacterioferritinAdd BLAST | 156 |
Interactioni
Subunit structurei
Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited.
By similarityStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P22759 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P22759 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 145 | Ferritin-like diironPROSITE-ProRule annotationAdd BLAST | 145 |
Sequence similaritiesi
Belongs to the bacterioferritin family.Curated
Family and domain databases
CDDi | cd00907, Bacterioferritin, 1 hit |
Gene3Di | 1.20.1260.10, 1 hit |
InterProi | View protein in InterPro IPR002024, Bacterioferritin IPR012347, Ferritin-like IPR009040, Ferritin-like_diiron IPR009078, Ferritin-like_SF IPR008331, Ferritin_DPS_dom |
Pfami | View protein in Pfam PF00210, Ferritin, 1 hit |
PIRSFi | PIRSF002560, Bacterioferritin, 1 hit |
PRINTSi | PR00601, BACFERRITIN |
SUPFAMi | SSF47240, SSF47240, 1 hit |
TIGRFAMsi | TIGR00754, bfr, 1 hit |
PROSITEi | View protein in PROSITE PS00549, BACTERIOFERRITIN, 1 hit PS50905, FERRITIN_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
P22759-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKGDKIVIQH LNKILGNELI AINQYFLHAR MYEDWGLEKL GKHEYHESID
60 70 80 90 100
EMKHADKLIK RILFLEGLPN LQELGKLLIG EHTKEMLECD LKLEQAGLPD
110 120 130 140 150
LKAAIAYCES VGDYASRELL EDILESEEDH IDWLETQLDL IDKIGLENYL
QSQMDE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M83692 Genomic DNA Translation: AAA22121.1 |
PIRi | A41983 |
RefSeqi | WP_012700041.1, NZ_FPKM01000033.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M83692 Genomic DNA Translation: AAA22121.1 |
PIRi | A41983 |
RefSeqi | WP_012700041.1, NZ_FPKM01000033.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SOF | X-ray | 2.60 | A/B/C/D/E/F/G/H | 1-156 | [»] | |
2FKZ | X-ray | 2.00 | A/B/C/D/E/F/G/H | 1-155 | [»] | |
2FL0 | X-ray | 2.70 | A/B/C/D/E/F/G/H | 1-155 | [»] | |
SMRi | P22759 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P22759 |
Family and domain databases
CDDi | cd00907, Bacterioferritin, 1 hit |
Gene3Di | 1.20.1260.10, 1 hit |
InterProi | View protein in InterPro IPR002024, Bacterioferritin IPR012347, Ferritin-like IPR009040, Ferritin-like_diiron IPR009078, Ferritin-like_SF IPR008331, Ferritin_DPS_dom |
Pfami | View protein in Pfam PF00210, Ferritin, 1 hit |
PIRSFi | PIRSF002560, Bacterioferritin, 1 hit |
PRINTSi | PR00601, BACFERRITIN |
SUPFAMi | SSF47240, SSF47240, 1 hit |
TIGRFAMsi | TIGR00754, bfr, 1 hit |
PROSITEi | View protein in PROSITE PS00549, BACTERIOFERRITIN, 1 hit PS50905, FERRITIN_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BFR_AZOVI | |
Accessioni | P22759Primary (citable) accession number: P22759 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | May 1, 1992 | |
Last modified: | August 12, 2020 | |
This is version 104 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families