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Protein

Granulysin

Gene

GNLY

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

GO - Biological processi

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Fungicide

Enzyme and pathway databases

ReactomeiR-HSA-6803157 Antimicrobial peptides

Protein family/group databases

TCDBi1.C.35.3.2 the amoebapore (amoebapore) family

Names & Taxonomyi

Protein namesi
Recommended name:
Granulysin
Alternative name(s):
Lymphokine LAG-2
Protein NKG5
T-cell activation protein 519
Gene namesi
Name:GNLY
Synonyms:LAG2, NKG5, TLA519
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115523.16
HGNCiHGNC:4414 GNLY
MIMi188855 gene
neXtProtiNX_P22749

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi10578
OpenTargetsiENSG00000115523
PharmGKBiPA28793

Chemistry databases

DrugBankiDB03434 3[N-Morpholino]Propane Sulfonic Acid

Polymorphism and mutation databases

BioMutaiGNLY
DMDMi116242500

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000003165923 – 145GranulysinAdd BLAST123

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi69 ↔ 132
Disulfide bondi96 ↔ 107

Post-translational modificationi

A 9 kDa form is produced by proteolytic processing of a 15 kDa protein.1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP22749
PeptideAtlasiP22749
PRIDEiP22749
ProteomicsDBi54035
54036 [P22749-2]

PTM databases

iPTMnetiP22749
PhosphoSitePlusiP22749

Expressioni

Tissue specificityi

Expressed in natural killer and T-cells.

Inductioni

By T-cell growth factor and IL2/interleukin-2.

Gene expression databases

BgeeiENSG00000115523 Expressed in 129 organ(s), highest expression level in leukocyte
CleanExiHS_GNLY
ExpressionAtlasiP22749 baseline and differential
GenevisibleiP22749 HS

Organism-specific databases

HPAiCAB025186
HPA058021

Interactioni

Protein-protein interaction databases

BioGridi115829, 1 interactor
IntActiP22749, 2 interactors
STRINGi9606.ENSP00000263863

Structurei

Secondary structure

1145
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP22749
SMRiP22749
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22749

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 142Saposin B-typePROSITE-ProRule annotationAdd BLAST81

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J70I Eukaryota
ENOG41117YV LUCA
GeneTreeiENSGT00390000002975
HOGENOMiHOG000112748
HOVERGENiHBG005866
InParanoidiP22749
PhylomeDBiP22749
TreeFamiTF342210

Family and domain databases

InterProiView protein in InterPro
IPR038847 Granulysin-like
IPR011001 Saposin-like
IPR008139 SaposinB_dom
PANTHERiPTHR15541 PTHR15541, 1 hit
SMARTiView protein in SMART
SM00741 SapB, 1 hit
SUPFAMiSSF47862 SSF47862, 1 hit
PROSITEiView protein in PROSITE
PS50015 SAP_B, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform Long (identifier: P22749-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATWALLLLA AMLLGNPGLV FSRLSPEYYD LARAHLRDEE KSCPCLAQEG
60 70 80 90 100
PQGDLLTKTQ ELGRDYRTCL TIVQKLKKMV DKPTQRSVSN AATRVCRTGR
110 120 130 140
SRWRDVCRNF MRRYQSRVTQ GLVAGETAQQ ICEDLRLCIP STGPL
Length:145
Mass (Da):16,374
Last modified:October 17, 2006 - v3
Checksum:iCEC5432FFBDBA1F4
GO
Isoform Short (identifier: P22749-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MATWALLLLAAMLLGNP → ME

Show »
Length:130
Mass (Da):14,853
Checksum:i6F5238713B905CF8
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B4E3H9B4E3H9_HUMAN
cDNA FLJ51776, highly similar to Gr...
GNLY
172Annotation score:
H0YDW8H0YDW8_HUMAN
Granulysin
GNLY
187Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31L → P in CAA28715 (PubMed:2434598).Curated1
Sequence conflicti39E → G in CAA28715 (PubMed:2434598).Curated1
Sequence conflicti46 – 47LA → G in CAA28715 (PubMed:2434598).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027868119T → I2 PublicationsCorresponds to variant dbSNP:rs11127Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0060161 – 17MATWA…LLGNP → ME in isoform Short. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05044 mRNA Translation: CAA28715.1
X54101 mRNA Translation: CAA38035.1
M85276 Genomic DNA Translation: AAA59935.1
CR541859 mRNA Translation: CAG46657.1
BC023576 mRNA Translation: AAH23576.1
CCDSiCCDS1984.1 [P22749-1]
CCDS46354.1 [P22749-2]
PIRiA27562
I54504
RefSeqiNP_001289687.1, NM_001302758.1
NP_006424.2, NM_006433.4 [P22749-1]
NP_036615.2, NM_012483.3 [P22749-2]
UniGeneiHs.105806

Genome annotation databases

EnsembliENST00000263863; ENSP00000263863; ENSG00000115523 [P22749-1]
ENST00000409696; ENSP00000387116; ENSG00000115523 [P22749-2]
GeneIDi10578
KEGGihsa:10578
UCSCiuc002sql.5 human [P22749-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05044 mRNA Translation: CAA28715.1
X54101 mRNA Translation: CAA38035.1
M85276 Genomic DNA Translation: AAA59935.1
CR541859 mRNA Translation: CAG46657.1
BC023576 mRNA Translation: AAH23576.1
CCDSiCCDS1984.1 [P22749-1]
CCDS46354.1 [P22749-2]
PIRiA27562
I54504
RefSeqiNP_001289687.1, NM_001302758.1
NP_006424.2, NM_006433.4 [P22749-1]
NP_036615.2, NM_012483.3 [P22749-2]
UniGeneiHs.105806

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L9LX-ray0.92A63-136[»]
ProteinModelPortaliP22749
SMRiP22749
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115829, 1 interactor
IntActiP22749, 2 interactors
STRINGi9606.ENSP00000263863

Chemistry databases

DrugBankiDB03434 3[N-Morpholino]Propane Sulfonic Acid

Protein family/group databases

TCDBi1.C.35.3.2 the amoebapore (amoebapore) family

PTM databases

iPTMnetiP22749
PhosphoSitePlusiP22749

Polymorphism and mutation databases

BioMutaiGNLY
DMDMi116242500

Proteomic databases

PaxDbiP22749
PeptideAtlasiP22749
PRIDEiP22749
ProteomicsDBi54035
54036 [P22749-2]

Protocols and materials databases

DNASUi10578
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263863; ENSP00000263863; ENSG00000115523 [P22749-1]
ENST00000409696; ENSP00000387116; ENSG00000115523 [P22749-2]
GeneIDi10578
KEGGihsa:10578
UCSCiuc002sql.5 human [P22749-1]

Organism-specific databases

CTDi10578
DisGeNETi10578
EuPathDBiHostDB:ENSG00000115523.16
GeneCardsiGNLY
HGNCiHGNC:4414 GNLY
HPAiCAB025186
HPA058021
MIMi188855 gene
neXtProtiNX_P22749
OpenTargetsiENSG00000115523
PharmGKBiPA28793
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J70I Eukaryota
ENOG41117YV LUCA
GeneTreeiENSGT00390000002975
HOGENOMiHOG000112748
HOVERGENiHBG005866
InParanoidiP22749
PhylomeDBiP22749
TreeFamiTF342210

Enzyme and pathway databases

ReactomeiR-HSA-6803157 Antimicrobial peptides

Miscellaneous databases

ChiTaRSiGNLY human
EvolutionaryTraceiP22749
GeneWikiiGNLY
GenomeRNAii10578
PROiPR:P22749
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115523 Expressed in 129 organ(s), highest expression level in leukocyte
CleanExiHS_GNLY
ExpressionAtlasiP22749 baseline and differential
GenevisibleiP22749 HS

Family and domain databases

InterProiView protein in InterPro
IPR038847 Granulysin-like
IPR011001 Saposin-like
IPR008139 SaposinB_dom
PANTHERiPTHR15541 PTHR15541, 1 hit
SMARTiView protein in SMART
SM00741 SapB, 1 hit
SUPFAMiSSF47862 SSF47862, 1 hit
PROSITEiView protein in PROSITE
PS50015 SAP_B, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGNLY_HUMAN
AccessioniPrimary (citable) accession number: P22749
Secondary accession number(s): P09325, Q6GU08
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 17, 2006
Last modified: September 12, 2018
This is version 169 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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