UniProtKB - P22732 (GTR5_HUMAN)
Protein
Solute carrier family 2, facilitated glucose transporter member 5
Gene
SLC2A5
Organism
Homo sapiens (Human)
Status
Functioni
Functions as a fructose transporter that has only low activity with other monosaccharides (PubMed:8333543, PubMed:16186102, PubMed:28083649). Can mediate the uptake of 2-deoxyglucose, but with low efficiency (PubMed:1695905). Essential for fructose uptake in the small intestine (By similarity). Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose (By similarity). Required for the development of high blood pressure in response to high dietary fructose intake (By similarity).By similarity4 Publications
Catalytic activityi
Activity regulationi
The uptake of 2-deoxyglucose is inhibited by cytochalasin B.1 Publication
Kineticsi
- KM=30.1 mM for fructose1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 32 | FructoseBy similarity | 1 | |
Binding sitei | 167 | FructoseBy similarity | 1 | |
Binding sitei | 288 | FructoseBy similarity | 1 | |
Binding sitei | 387 | FructoseBy similarity | 1 |
GO - Molecular functioni
- fructose binding Source: UniProtKB
- fructose transmembrane transporter activity Source: UniProtKB
- glucose transmembrane transporter activity Source: ProtInc
GO - Biological processi
- carbohydrate metabolic process Source: ProtInc
- cellular response to fructose stimulus Source: UniProtKB
- fructose import across plasma membrane Source: UniProtKB
- fructose transmembrane transport Source: UniProtKB
- glucose transmembrane transport Source: ProtInc
- intestinal hexose absorption Source: Reactome
- neutrophil degranulation Source: Reactome
- regulation of systemic arterial blood pressure mediated by a chemical signal Source: UniProtKB
- response to fructose Source: UniProtKB
Keywordsi
Biological process | Sugar transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | P22732 |
Reactomei | R-HSA-6798695, Neutrophil degranulation R-HSA-8981373, Intestinal hexose absorption |
Protein family/group databases
TCDBi | 2.A.1.1.13, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Solute carrier family 2, facilitated glucose transporter member 5CuratedAlternative name(s): Fructose transporterCurated Glucose transporter type 5, small intestine1 Publication Short name: GLUT-51 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000142583.17 |
HGNCi | HGNC:11010, SLC2A5 |
MIMi | 138230, gene |
neXtProti | NX_P22732 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Multi-pass membrane protein By similarity
- Cell membrane 4 Publications; Multi-pass membrane protein By similarity
- sarcolemma By similarity
Note: Localized on the apical membrane of jejunum villi, but also on lateral plasma membranes of the villi. Transport to the cell membrane is dependent on RAB11A.By similarity
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
- sarcolemma Source: UniProtKB
Other locations
- specific granule membrane Source: Reactome
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 18 | CytoplasmicBy similarityAdd BLAST | 18 | |
Transmembranei | 19 – 39 | Helical; Name=1By similarityAdd BLAST | 21 | |
Topological domaini | 40 – 68 | ExtracellularBy similarityAdd BLAST | 29 | |
Transmembranei | 69 – 91 | Helical; Name=2By similarityAdd BLAST | 23 | |
Topological domaini | 92 – 98 | CytoplasmicBy similarity | 7 | |
Transmembranei | 99 – 119 | Helical; Name=3By similarityAdd BLAST | 21 | |
Topological domaini | 120 – 126 | ExtracellularBy similarity | 7 | |
Transmembranei | 127 – 149 | Helical; Name=4By similarityAdd BLAST | 23 | |
Topological domaini | 150 – 161 | CytoplasmicBy similarityAdd BLAST | 12 | |
Transmembranei | 162 – 182 | Helical; Name=5By similarityAdd BLAST | 21 | |
Topological domaini | 183 – 192 | ExtracellularBy similarity | 10 | |
Transmembranei | 193 – 213 | Helical; Name=6By similarityAdd BLAST | 21 | |
Topological domaini | 214 – 277 | CytoplasmicBy similarityAdd BLAST | 64 | |
Transmembranei | 278 – 298 | Helical; Name=7By similarityAdd BLAST | 21 | |
Topological domaini | 299 – 313 | ExtracellularBy similarityAdd BLAST | 15 | |
Transmembranei | 314 – 334 | Helical; Name=8By similarityAdd BLAST | 21 | |
Topological domaini | 335 – 342 | CytoplasmicBy similarity | 8 | |
Transmembranei | 343 – 363 | Helical; Name=9By similarityAdd BLAST | 21 | |
Topological domaini | 364 – 371 | ExtracellularBy similarity | 8 | |
Transmembranei | 372 – 394 | Helical; Name=10By similarityAdd BLAST | 23 | |
Topological domaini | 395 – 412 | CytoplasmicBy similarityAdd BLAST | 18 | |
Transmembranei | 413 – 433 | Helical; Name=11By similarityAdd BLAST | 21 | |
Topological domaini | 434 – 439 | ExtracellularBy similarity | 6 | |
Transmembranei | 440 – 460 | Helical; Name=12By similarityAdd BLAST | 21 | |
Topological domaini | 461 – 501 | CytoplasmicBy similarityAdd BLAST | 41 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 6518 |
OpenTargetsi | ENSG00000142583 |
PharmGKBi | PA35880 |
Miscellaneous databases
Pharosi | P22732, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5875 |
DrugBanki | DB01914, D-glucose DB09502, Fludeoxyglucose (18F) DB09344, Invert sugar |
Polymorphism and mutation databases
BioMutai | SLC2A5 |
DMDMi | 121764 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050369 | 1 – 501 | Solute carrier family 2, facilitated glucose transporter member 5Add BLAST | 501 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Glycosylationi | 51 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Acetylation, GlycoproteinProteomic databases
jPOSTi | P22732 |
MassIVEi | P22732 |
PaxDbi | P22732 |
PeptideAtlasi | P22732 |
PRIDEi | P22732 |
ProteomicsDBi | 54029 [P22732-1] 54030 [P22732-2] |
PTM databases
GlyGeni | P22732, 1 site |
iPTMneti | P22732 |
PhosphoSitePlusi | P22732 |
Expressioni
Tissue specificityi
Detected in skeletal muscle, and in jejunum brush border membrane and basolateral membrane (at protein level) (PubMed:7619085). Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.2 Publications
Inductioni
By forskolin in Caco-2 cells.1 Publication
Gene expression databases
Bgeei | ENSG00000142583, Expressed in jejunal mucosa and 200 other tissues |
ExpressionAtlasi | P22732, baseline and differential |
Genevisiblei | P22732, HS |
Organism-specific databases
HPAi | ENSG00000142583, Tissue enhanced (bone marrow, intestine) |
Interactioni
Binary interactionsi
Hide detailsP22732
Protein-protein interaction databases
BioGRIDi | 112409, 24 interactors |
IntActi | P22732, 23 interactors |
STRINGi | 9606.ENSP00000366641 |
Chemistry databases
BindingDBi | P22732 |
Miscellaneous databases
RNActi | P22732, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 296 – 298 | Fructose bindingBy similarity | 3 | |
Regioni | 419 – 420 | Fructose bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0569, Eukaryota |
GeneTreei | ENSGT00940000156846 |
HOGENOMi | CLU_001265_30_5_1 |
InParanoidi | P22732 |
OMAi | GCIEFTG |
OrthoDBi | 326501at2759 |
PhylomeDBi | P22732 |
TreeFami | TF313762 |
Family and domain databases
InterProi | View protein in InterPro IPR002442, Fru_transpt_5 IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR003663, Sugar/inositol_transpt IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
PRINTSi | PR01194, GLUCTRSPORT5 PR00171, SUGRTRNSPORT |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00879, SP, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 1 hit PS00217, SUGAR_TRANSPORT_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P22732-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEQQDQSMKE GRLTLVLALA TLIAAFGSSF QYGYNVAAVN SPALLMQQFY
60 70 80 90 100
NETYYGRTGE FMEDFPLTLL WSVTVSMFPF GGFIGSLLVG PLVNKFGRKG
110 120 130 140 150
ALLFNNIFSI VPAILMGCSR VATSFELIII SRLLVGICAG VSSNVVPMYL
160 170 180 190 200
GELAPKNLRG ALGVVPQLFI TVGILVAQIF GLRNLLANVD GWPILLGLTG
210 220 230 240 250
VPAALQLLLL PFFPESPRYL LIQKKDEAAA KKALQTLRGW DSVDREVAEI
260 270 280 290 300
RQEDEAEKAA GFISVLKLFR MRSLRWQLLS IIVLMGGQQL SGVNAIYYYA
310 320 330 340 350
DQIYLSAGVP EEHVQYVTAG TGAVNVVMTF CAVFVVELLG RRLLLLLGFS
360 370 380 390 400
ICLIACCVLT AALALQDTVS WMPYISIVCV ISYVIGHALG PSPIPALLIT
410 420 430 440 450
EIFLQSSRPS AFMVGGSVHW LSNFTVGLIF PFIQEGLGPY SFIVFAVICL
460 470 480 490 500
LTTIYIFLIV PETKAKTFIE INQIFTKMNK VSEVYPEKEE LKELPPVTSE
Q
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EJZ0 | K7EJZ0_HUMAN | Fructose transporter | SLC2A5 | 166 | Annotation score: | ||
K7EIT1 | K7EIT1_HUMAN | Fructose transporter | SLC2A5 | 190 | Annotation score: | ||
K7EQI3 | K7EQI3_HUMAN | Solute carrier family 2, facilitate... | SLC2A5 | 139 | Annotation score: | ||
K7EJR1 | K7EJR1_HUMAN | Solute carrier family 2, facilitate... | SLC2A5 | 81 | Annotation score: |
Sequence cautioni
The sequence AAB60641 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 202 | P → G in AAB60641 (PubMed:8037665).Curated | 1 |
Mass spectrometryi
Molecular mass is 54973.98 Da. Determined by MALDI. 1 Publication
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_081827 | 296 | I → V Polymorphism; does not affect fructose transport. 1 PublicationCorresponds to variant dbSNP:rs1451503051Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042031 | 192 – 501 | WPILL…VTSEQ → EFRTSREHPHPFTTTLGPLL VFQSHHHRTGLSADWSLLTG WMSLGGPSCPEPT in isoform 2. 2 PublicationsAdd BLAST | 310 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55531 mRNA Translation: AAA52570.1 U11843 , U05344, U11839, U11840, U11841, U11842 Genomic DNA Translation: AAB60641.1 Sequence problems. AK289849 mRNA Translation: BAF82538.1 AK290398 mRNA Translation: BAF83087.1 AL158048 Genomic DNA No translation available. CH471130 Genomic DNA Translation: EAW71610.1 BC001692 mRNA Translation: AAH01692.1 BC001820 mRNA Translation: AAH01820.1 BC035878 mRNA Translation: AAH35878.1 |
CCDSi | CCDS44054.1 [P22732-2] CCDS99.1 [P22732-1] |
PIRi | A36629 G02864 |
RefSeqi | NP_001129057.1, NM_001135585.1 [P22732-2] NP_001315548.1, NM_001328619.1 [P22732-1] NP_003030.1, NM_003039.2 [P22732-1] XP_005263548.1, XM_005263491.3 XP_016857622.1, XM_017002133.1 XP_016857623.1, XM_017002134.1 XP_016857624.1, XM_017002135.1 XP_016857625.1, XM_017002136.1 XP_016857626.1, XM_017002137.1 XP_016857627.1, XM_017002138.1 XP_016857629.1, XM_017002140.1 XP_016857630.1, XM_017002141.1 |
Genome annotation databases
Ensembli | ENST00000377414; ENSP00000366631; ENSG00000142583 [P22732-2] ENST00000377424; ENSP00000366641; ENSG00000142583 [P22732-1] |
GeneIDi | 6518 |
KEGGi | hsa:6518 |
UCSCi | uc001apo.4, human [P22732-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55531 mRNA Translation: AAA52570.1 U11843 , U05344, U11839, U11840, U11841, U11842 Genomic DNA Translation: AAB60641.1 Sequence problems. AK289849 mRNA Translation: BAF82538.1 AK290398 mRNA Translation: BAF83087.1 AL158048 Genomic DNA No translation available. CH471130 Genomic DNA Translation: EAW71610.1 BC001692 mRNA Translation: AAH01692.1 BC001820 mRNA Translation: AAH01820.1 BC035878 mRNA Translation: AAH35878.1 |
CCDSi | CCDS44054.1 [P22732-2] CCDS99.1 [P22732-1] |
PIRi | A36629 G02864 |
RefSeqi | NP_001129057.1, NM_001135585.1 [P22732-2] NP_001315548.1, NM_001328619.1 [P22732-1] NP_003030.1, NM_003039.2 [P22732-1] XP_005263548.1, XM_005263491.3 XP_016857622.1, XM_017002133.1 XP_016857623.1, XM_017002134.1 XP_016857624.1, XM_017002135.1 XP_016857625.1, XM_017002136.1 XP_016857626.1, XM_017002137.1 XP_016857627.1, XM_017002138.1 XP_016857629.1, XM_017002140.1 XP_016857630.1, XM_017002141.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YG1 | model | - | A | 1-501 | [»] | |
SMRi | P22732 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112409, 24 interactors |
IntActi | P22732, 23 interactors |
STRINGi | 9606.ENSP00000366641 |
Chemistry databases
BindingDBi | P22732 |
ChEMBLi | CHEMBL5875 |
DrugBanki | DB01914, D-glucose DB09502, Fludeoxyglucose (18F) DB09344, Invert sugar |
Protein family/group databases
TCDBi | 2.A.1.1.13, the major facilitator superfamily (mfs) |
PTM databases
GlyGeni | P22732, 1 site |
iPTMneti | P22732 |
PhosphoSitePlusi | P22732 |
Polymorphism and mutation databases
BioMutai | SLC2A5 |
DMDMi | 121764 |
Proteomic databases
jPOSTi | P22732 |
MassIVEi | P22732 |
PaxDbi | P22732 |
PeptideAtlasi | P22732 |
PRIDEi | P22732 |
ProteomicsDBi | 54029 [P22732-1] 54030 [P22732-2] |
Protocols and materials databases
Antibodypediai | 1404, 399 antibodies |
DNASUi | 6518 |
Genome annotation databases
Ensembli | ENST00000377414; ENSP00000366631; ENSG00000142583 [P22732-2] ENST00000377424; ENSP00000366641; ENSG00000142583 [P22732-1] |
GeneIDi | 6518 |
KEGGi | hsa:6518 |
UCSCi | uc001apo.4, human [P22732-1] |
Organism-specific databases
CTDi | 6518 |
DisGeNETi | 6518 |
EuPathDBi | HostDB:ENSG00000142583.17 |
GeneCardsi | SLC2A5 |
HGNCi | HGNC:11010, SLC2A5 |
HPAi | ENSG00000142583, Tissue enhanced (bone marrow, intestine) |
MIMi | 138230, gene |
neXtProti | NX_P22732 |
OpenTargetsi | ENSG00000142583 |
PharmGKBi | PA35880 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0569, Eukaryota |
GeneTreei | ENSGT00940000156846 |
HOGENOMi | CLU_001265_30_5_1 |
InParanoidi | P22732 |
OMAi | GCIEFTG |
OrthoDBi | 326501at2759 |
PhylomeDBi | P22732 |
TreeFami | TF313762 |
Enzyme and pathway databases
PathwayCommonsi | P22732 |
Reactomei | R-HSA-6798695, Neutrophil degranulation R-HSA-8981373, Intestinal hexose absorption |
Miscellaneous databases
BioGRID-ORCSi | 6518, 6 hits in 839 CRISPR screens |
ChiTaRSi | SLC2A5, human |
GenomeRNAii | 6518 |
Pharosi | P22732, Tbio |
PROi | PR:P22732 |
RNActi | P22732, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000142583, Expressed in jejunal mucosa and 200 other tissues |
ExpressionAtlasi | P22732, baseline and differential |
Genevisiblei | P22732, HS |
Family and domain databases
InterProi | View protein in InterPro IPR002442, Fru_transpt_5 IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR003663, Sugar/inositol_transpt IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF00083, Sugar_tr, 1 hit |
PRINTSi | PR01194, GLUCTRSPORT5 PR00171, SUGRTRNSPORT |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00879, SP, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 1 hit PS00217, SUGAR_TRANSPORT_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GTR5_HUMAN | |
Accessioni | P22732Primary (citable) accession number: P22732 Secondary accession number(s): Q14770, Q5T977, Q8IVB3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | August 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 202 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations