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Protein

Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type

Gene

Ca-P60A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi304Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi305Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi307Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi309Calcium 2By similarity1
Active sitei3514-aspartylphosphate intermediateBy similarity1
Metal bindingi703MagnesiumBy similarity1
Metal bindingi707MagnesiumBy similarity1
Metal bindingi768Calcium 1By similarity1
Metal bindingi771Calcium 1By similarity1
Metal bindingi796Calcium 2By similarity1
Metal bindingi799Calcium 1By similarity1
Metal bindingi800Calcium 1By similarity1
Metal bindingi800Calcium 2By similarity1
Metal bindingi908Calcium 1By similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • proton-exporting ATPase activity, phosphorylative mechanism Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-418359 Reduction of cytosolic Ca++ levels
R-DME-5578775 Ion homeostasis
R-DME-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type (EC:3.6.3.8)
Alternative name(s):
Calcium ATPase at 60A
Calcium pump
Gene namesi
Name:Ca-P60A
ORF Names:CG3725
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0263006 Ca-P60A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicBy similarityAdd BLAST48
Transmembranei49 – 69Helical; Name=1By similarityAdd BLAST21
Topological domaini70 – 89LumenalBy similarityAdd BLAST20
Transmembranei90 – 110Helical; Name=2By similarityAdd BLAST21
Topological domaini111 – 253CytoplasmicBy similarityAdd BLAST143
Transmembranei254 – 273Helical; Name=3By similarityAdd BLAST20
Topological domaini274 – 295LumenalBy similarityAdd BLAST22
Transmembranei296 – 313Helical; Name=4By similarityAdd BLAST18
Topological domaini314 – 757CytoplasmicBy similarityAdd BLAST444
Transmembranei758 – 777Helical; Name=5By similarityAdd BLAST20
Topological domaini778 – 787LumenalBy similarity10
Transmembranei788 – 808Helical; Name=6By similarityAdd BLAST21
Topological domaini809 – 828CytoplasmicBy similarityAdd BLAST20
Transmembranei829 – 851Helical; Name=7By similarityAdd BLAST23
Topological domaini852 – 897LumenalBy similarityAdd BLAST46
Transmembranei898 – 917Helical; Name=8By similarityAdd BLAST20
Topological domaini918 – 930CytoplasmicBy similarityAdd BLAST13
Transmembranei931 – 949Helical; Name=9By similarityAdd BLAST19
Topological domaini950 – 964LumenalBy similarityAdd BLAST15
Transmembranei965 – 985Helical; Name=10By similarityAdd BLAST21
Topological domaini986 – 1020CytoplasmicBy similarityAdd BLAST35

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462081 – 1020Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum typeAdd BLAST1020

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei240Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP22700
PRIDEiP22700

PTM databases

iPTMnetiP22700

Expressioni

Gene expression databases

BgeeiFBgn0263006 Expressed in 49 organ(s), highest expression level in head
ExpressionAtlasiP22700 baseline and differential
GenevisibleiP22700 DM

Interactioni

Subunit structurei

Interacts with SclA and SclB.1 Publication

Protein-protein interaction databases

BioGridi72161, 127 interactors
DIPiDIP-20129N
IntActiP22700, 6 interactors
STRINGi7227.FBpp0072125

Structurei

3D structure databases

ProteinModelPortaliP22700
SMRiP22700
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202 Eukaryota
COG0474 LUCA
GeneTreeiENSGT00890000139334
InParanoidiP22700
KOiK05853
OrthoDBiEOG091G01LE
PhylomeDBiP22700

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR005782 P-type_ATPase_IIA
IPR001757 P_typ_ATPase
IPR030332 SERCA1/2
PANTHERiPTHR42861:SF18 PTHR42861:SF18, 1 hit
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01116 ATPase-IIA1_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform B (identifier: P22700-1) [UniParc]FASTAAdd to basket
Also known as: C, D, E, F, G, H

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEDGHSKTVE QSLNFFGTDP ERGLTLDQIK ANQKKYGPNE LPTEEGKSIW
60 70 80 90 100
QLVLEQFDDL LVKILLLAAI ISFVLALFEE HEETFTAFVE PLVILLILIA
110 120 130 140 150
NAVVGVWQER NAESAIEALK EYEPEMGKVV RQDKSGIQKV RAKEIVPGDL
160 170 180 190 200
VEVSVGDKIP ADIRITHIYS TTLRIDQSIL TGESVSVIKH TDAIPDPRAV
210 220 230 240 250
NQDKKNILFS GTNVAAGKAR GVVIGTGLST AIGKIRTEMS ETEEIKTPLQ
260 270 280 290 300
QKLDEFGEQL SKVISVICVA VWAINIGHFN DPAHGGSWIK GAIYYFKIAV
310 320 330 340 350
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS
360 370 380 390 400
DKTGTLTTNQ MSVSRMFIFD KVEGNDSSFL EFEMTGSTYE PIGEVFLNGQ
410 420 430 440 450
RIKAADYDTL QELSTICIMC NDSAIDYNEF KQAFEKVGEA TETALIVLAE
460 470 480 490 500
KLNSFSVNKS GLDRRSAAIA CRGEIETKWK KEFTLEFSRD RKSMSSYCTP
510 520 530 540 550
LKASRLGTGP KLFVKGAPEG VLERCTHARV GTTKVPLTSA LKAKILALTG
560 570 580 590 600
QYGTGRDTLR CLALAVADSP MKPDEMDLGD STKFYQYEVN LTFVGVVGML
610 620 630 640 650
DPPRKEVFDS IVRCRAAGIR VIVITGDNKA TAEAICRRIG VFAEDEDTTG
660 670 680 690 700
KSYSGREFDD LSPTEQKAAV ARSRLFSRVE PQHKSKIVEF LQSMNEISAM
710 720 730 740 750
TGDGVNDAPA LKKAEIGIAM GSGTAVAKSA AEMVLADDNF SSIVSAVEEG
760 770 780 790 800
RAIYNNMKQF IRYLISSNIG EVVSIFLTAA LGLPEALIPV QLLWVNLVTD
810 820 830 840 850
GLPATALGFN PPDLDIMEKP PRKADEGLIS GWLFFRYMAI GFYVGAATVG
860 870 880 890 900
AAAWWFVFSD EGPKLSYWQL THHLSCLGGG DEFKGVDCKI FSDPHAMTMA
910 920 930 940 950
LSVLVTIEML NAMNSLSENQ SLITMPPWCN LWLIGSMALS FTLHFVILYV
960 970 980 990 1000
DVLSTVFQVT PLSAEEWITV MKFSIPVVLL DETLKFVARK IADGESPIYK
1010 1020
MHGIVLMWAV FFGLLYAMML
Note: No experimental confirmation available.
Length:1,020
Mass (Da):111,701
Last modified:December 1, 2000 - v2
Checksum:i6A62D350BF316984
GO
Isoform A (identifier: P22700-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     994-1020: GESPIYKMHGIVLMWAVFFGLLYAMML → VPDVVVDRM

Show »
Length:1,002
Mass (Da):109,611
Checksum:i463400C1967BD7D7
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0B4LGB7A0A0B4LGB7_DROME
Calcium-transporting ATPase
SERCA CG3725, Dmel_CG3725
999Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158K → R in AAL13694 (PubMed:12537569).Curated1
Sequence conflicti302L → V in AAB00735 (PubMed:2148477).Curated1
Sequence conflicti357T → L in CAA35505 (PubMed:2557235).Curated1
Sequence conflicti495S → P in AAL13694 (PubMed:12537569).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010297994 – 1020GESPI…YAMML → VPDVVVDRM in isoform A. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62892 mRNA Translation: AAB00735.1
AE013599 Genomic DNA Translation: AAF47101.1
AE013599 Genomic DNA Translation: AAF47102.1
AE013599 Genomic DNA Translation: AAF47103.1
AE013599 Genomic DNA Translation: AAM68278.1
AE013599 Genomic DNA Translation: AAM68279.1
AE013599 Genomic DNA Translation: AAM68280.1
AE013599 Genomic DNA Translation: AAM68281.1
AY058465 mRNA Translation: AAL13694.1
X17472 mRNA Translation: CAA35505.1
PIRiA36691
RefSeqiNP_476832.1, NM_057484.3 [P22700-2]
NP_726381.1, NM_166633.2 [P22700-1]
NP_726382.1, NM_166634.2 [P22700-1]
NP_726383.1, NM_166635.2 [P22700-1]
NP_726384.1, NM_166636.2 [P22700-1]
NP_726385.1, NM_166637.2 [P22700-1]
NP_726386.1, NM_166638.2 [P22700-1]
NP_726387.1, NM_166639.2 [P22700-1]
UniGeneiDm.3812

Genome annotation databases

EnsemblMetazoaiFBtr0072211; FBpp0072120; FBgn0263006 [P22700-1]
FBtr0072212; FBpp0072121; FBgn0263006 [P22700-1]
FBtr0072213; FBpp0072122; FBgn0263006 [P22700-1]
FBtr0072214; FBpp0072123; FBgn0263006 [P22700-1]
FBtr0072215; FBpp0072124; FBgn0263006 [P22700-2]
FBtr0072216; FBpp0072125; FBgn0263006 [P22700-1]
FBtr0072217; FBpp0072126; FBgn0263006 [P22700-1]
FBtr0072218; FBpp0072127; FBgn0263006 [P22700-1]
GeneIDi49297
KEGGidme:Dmel_CG3725

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62892 mRNA Translation: AAB00735.1
AE013599 Genomic DNA Translation: AAF47101.1
AE013599 Genomic DNA Translation: AAF47102.1
AE013599 Genomic DNA Translation: AAF47103.1
AE013599 Genomic DNA Translation: AAM68278.1
AE013599 Genomic DNA Translation: AAM68279.1
AE013599 Genomic DNA Translation: AAM68280.1
AE013599 Genomic DNA Translation: AAM68281.1
AY058465 mRNA Translation: AAL13694.1
X17472 mRNA Translation: CAA35505.1
PIRiA36691
RefSeqiNP_476832.1, NM_057484.3 [P22700-2]
NP_726381.1, NM_166633.2 [P22700-1]
NP_726382.1, NM_166634.2 [P22700-1]
NP_726383.1, NM_166635.2 [P22700-1]
NP_726384.1, NM_166636.2 [P22700-1]
NP_726385.1, NM_166637.2 [P22700-1]
NP_726386.1, NM_166638.2 [P22700-1]
NP_726387.1, NM_166639.2 [P22700-1]
UniGeneiDm.3812

3D structure databases

ProteinModelPortaliP22700
SMRiP22700
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi72161, 127 interactors
DIPiDIP-20129N
IntActiP22700, 6 interactors
STRINGi7227.FBpp0072125

PTM databases

iPTMnetiP22700

Proteomic databases

PaxDbiP22700
PRIDEiP22700

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072211; FBpp0072120; FBgn0263006 [P22700-1]
FBtr0072212; FBpp0072121; FBgn0263006 [P22700-1]
FBtr0072213; FBpp0072122; FBgn0263006 [P22700-1]
FBtr0072214; FBpp0072123; FBgn0263006 [P22700-1]
FBtr0072215; FBpp0072124; FBgn0263006 [P22700-2]
FBtr0072216; FBpp0072125; FBgn0263006 [P22700-1]
FBtr0072217; FBpp0072126; FBgn0263006 [P22700-1]
FBtr0072218; FBpp0072127; FBgn0263006 [P22700-1]
GeneIDi49297
KEGGidme:Dmel_CG3725

Organism-specific databases

CTDi49297
FlyBaseiFBgn0263006 Ca-P60A

Phylogenomic databases

eggNOGiKOG0202 Eukaryota
COG0474 LUCA
GeneTreeiENSGT00890000139334
InParanoidiP22700
KOiK05853
OrthoDBiEOG091G01LE
PhylomeDBiP22700

Enzyme and pathway databases

ReactomeiR-DME-418359 Reduction of cytosolic Ca++ levels
R-DME-5578775 Ion homeostasis
R-DME-936837 Ion transport by P-type ATPases

Miscellaneous databases

GenomeRNAii49297
PROiPR:P22700

Gene expression databases

BgeeiFBgn0263006 Expressed in 49 organ(s), highest expression level in head
ExpressionAtlasiP22700 baseline and differential
GenevisibleiP22700 DM

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR005782 P-type_ATPase_IIA
IPR001757 P_typ_ATPase
IPR030332 SERCA1/2
PANTHERiPTHR42861:SF18 PTHR42861:SF18, 1 hit
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01116 ATPase-IIA1_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiATC1_DROME
AccessioniPrimary (citable) accession number: P22700
Secondary accession number(s): A4UZU0
, Q2MGN2, Q95TX1, Q9W1G2, Q9W1G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 1, 2000
Last modified: November 7, 2018
This is version 185 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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Main funding by: National Institutes of Health

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