UniProtKB - P22694 (KAPCB_HUMAN)
cAMP-dependent protein kinase catalytic subunit beta
PRKACB
Functioni
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 73 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 167 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 50 – 58 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- AMP-activated protein kinase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB
- cAMP-dependent protein kinase activity Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- protein serine kinase activity Source: RHEA
- protein threonine kinase activity Source: RHEA
- ubiquitin protein ligase binding Source: UniProtKB
GO - Biological processi
- activation of protein kinase A activity Source: Reactome
- adaptive immune response Source: Reactome
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: UniProtKB
- blood coagulation Source: Reactome
- cellular response to glucagon stimulus Source: Reactome
- chemical synaptic transmission Source: Reactome
- high-density lipoprotein particle assembly Source: Reactome
- negative regulation of inflammatory response to antigenic stimulus Source: Reactome
- negative regulation of meiotic cell cycle Source: Ensembl
- negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning Source: Ensembl
- neural tube closure Source: Ensembl
- protein kinase A signaling Source: GO_Central
- protein phosphorylation Source: UniProtKB
- regulation of protein processing Source: Ensembl
- renal water homeostasis Source: Reactome
- response to clozapine Source: Ensembl
- signal transduction Source: ProtInc
- stimulatory C-type lectin receptor signaling pathway Source: Reactome
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, cAMP, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.11, 2681 |
PathwayCommonsi | P22694 |
Reactomei | R-HSA-111931, PKA-mediated phosphorylation of CREB R-HSA-163358, PKA-mediated phosphorylation of key metabolic factors R-HSA-163560, Triglyceride catabolism R-HSA-163615, PKA activation R-HSA-164378, PKA activation in glucagon signalling R-HSA-180024, DARPP-32 events R-HSA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-HSA-392517, Rap1 signalling R-HSA-422356, Regulation of insulin secretion R-HSA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-HSA-4420097, VEGFA-VEGFR2 Pathway R-HSA-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-HSA-5610780, Degradation of GLI1 by the proteasome R-HSA-5610783, Degradation of GLI2 by the proteasome R-HSA-5610785, GLI3 is processed to GLI3R by the proteasome R-HSA-5610787, Hedgehog 'off' state R-HSA-5621575, CD209 (DC-SIGN) signaling R-HSA-5687128, MAPK6/MAPK4 signaling R-HSA-8853659, RET signaling R-HSA-8963896, HDL assembly R-HSA-9010642, ROBO receptors bind AKAP5 R-HSA-9634600, Regulation of glycolysis by fructose 2,6-bisphosphate metabolism R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production R-HSA-9664323, FCGR3A-mediated IL10 synthesis R-HSA-983231, Factors involved in megakaryocyte development and platelet production |
SABIO-RKi | P22694 |
SignaLinki | P22694 |
SIGNORi | P22694 |
Names & Taxonomyi
Protein namesi | Recommended name: cAMP-dependent protein kinase catalytic subunit beta (EC:2.7.11.11)Short name: PKA C-beta |
Gene namesi | Name:PRKACB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9381, PRKACB |
MIMi | 176892, gene |
neXtProti | NX_P22694 |
VEuPathDBi | HostDB:ENSG00000142875.19 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Nucleus
- Nucleus By similarity
Plasma membrane
- Cell membrane 1 Publication
Other locations
- Membrane Curated; Lipid-anchor Curated
Note: Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm.By similarity
Cytoskeleton
- centrosome Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cAMP-dependent protein kinase complex Source: UniProtKB
- ciliary base Source: Reactome
- perinuclear region of cytoplasm Source: Ensembl
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 5567 |
OpenTargetsi | ENSG00000142875 |
PharmGKBi | PA33749 |
Miscellaneous databases
Pharosi | P22694, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2918 |
DrugBanki | DB12010, Fostamatinib DB02482, Phosphonothreonine |
DrugCentrali | P22694 |
GuidetoPHARMACOLOGYi | 1477 |
Genetic variation databases
BioMutai | PRKACB |
DMDMi | 125210 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000086060 | 2 – 351 | cAMP-dependent protein kinase catalytic subunit betaAdd BLAST | 350 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Modified residuei | 3 | Deamidated asparagineBy similarity | 1 | |
Modified residuei | 11 | PhosphoserineBy similarity | 1 | |
Modified residuei | 69 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 140 | PhosphoserineBy similarity | 1 | |
Modified residuei | 198 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 322 | PhosphoserineBy similarity | 1 | |
Modified residuei | 331 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 339 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
EPDi | P22694 |
jPOSTi | P22694 |
MassIVEi | P22694 |
MaxQBi | P22694 |
PaxDbi | P22694 |
PeptideAtlasi | P22694 |
PRIDEi | P22694 |
ProteomicsDBi | 3296 54019 [P22694-1] 54020 [P22694-2] 54021 [P22694-3] 54022 [P22694-4] 54023 [P22694-5] 54024 [P22694-6] 54025 [P22694-7] 54026 [P22694-8] 54027 [P22694-9] |
PTM databases
iPTMneti | P22694 |
MetOSitei | P22694 |
PhosphoSitePlusi | P22694 |
SwissPalmi | P22694 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000142875, Expressed in caudate nucleus and 254 other tissues |
ExpressionAtlasi | P22694, baseline and differential |
Genevisiblei | P22694, HS |
Organism-specific databases
HPAi | ENSG00000142875, Tissue enhanced (brain) |
Interactioni
Subunit structurei
A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits (By similarity). The cAMP-dependent protein kinase catalytic subunit binds PJA2 (PubMed:21423175).
Interacts with GPKOW (PubMed:21880142).
By similarity2 PublicationsBinary interactionsi
Hide detailsP22694
Isoform 2 [P22694-2]
With | #Exp. | IntAct |
---|---|---|
GPKOW [Q92917] | 4 | EBI-5258763,EBI-746309 |
Isoform 8 [P22694-8]
With | #Exp. | IntAct |
---|---|---|
APP [P05067] | 3 | EBI-25937151,EBI-77613 |
GO - Molecular functioni
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 111554, 105 interactors |
IntActi | P22694, 73 interactors |
MINTi | P22694 |
STRINGi | 9606.ENSP00000359719 |
Chemistry databases
BindingDBi | P22694 |
Miscellaneous databases
RNActi | P22694, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 44 – 298 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 255 | |
Domaini | 299 – 351 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 53 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0616, Eukaryota |
GeneTreei | ENSGT00940000161169 |
HOGENOMi | CLU_000288_63_5_1 |
InParanoidi | P22694 |
OMAi | SSLFDHY |
OrthoDBi | 963519at2759 |
PhylomeDBi | P22694 |
TreeFami | TF313399 |
Family and domain databases
CDDi | cd14209, STKc_PKA, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR044109, STKc_PKA |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (10+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 10 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 10 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGNAATAKKG SEVESVKEFL AKAKEDFLKK WENPTQNNAG LEDFERKKTL
60 70 80 90 100
GTGSFGRVML VKHKATEQYY AMKILDKQKV VKLKQIEHTL NEKRILQAVN
110 120 130 140 150
FPFLVRLEYA FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ
160 170 180 190 200
IVLTFEYLHS LDLIYRDLKP ENLLIDHQGY IQVTDFGFAK RVKGRTWTLC
210 220 230 240 250
GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK
260 270 280 290 300
IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVS DIKTHKWFAT
310 320 330 340 350
TDWIAIYQRK VEAPFIPKFR GSGDTSNFDD YEEEDIRVSI TEKCAKEFGE
F
The sequence of this isoform differs from the canonical sequence as follows:
1-14: MGNAATAKKGSEVE → MSARKSSDASACSSSEI
79-111: Missing.
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WVC4 | A0A087WVC4_HUMAN | cAMP-dependent protein kinase | PRKACB | 338 | Annotation score: | ||
A0A0A0MS54 | A0A0A0MS54_HUMAN | cAMP-dependent protein kinase | PRKACB | 357 | Annotation score: | ||
B1APF9 | B1APF9_HUMAN | cAMP-dependent protein kinase catal... | PRKACB | 245 | Annotation score: | ||
B1APG3 | B1APG3_HUMAN | cAMP-dependent protein kinase catal... | PRKACB | 264 | Annotation score: | ||
B1APF7 | B1APF7_HUMAN | cAMP-dependent protein kinase catal... | PRKACB | 186 | Annotation score: | ||
B1APF8 | B1APF8_HUMAN | cAMP-dependent protein kinase catal... | PRKACB | 214 | Annotation score: | ||
B1APG0 | B1APG0_HUMAN | cAMP-dependent protein kinase catal... | PRKACB | 206 | Annotation score: | ||
B1APG2 | B1APG2_HUMAN | cAMP-dependent protein kinase catal... | PRKACB | 209 | Annotation score: | ||
B1APG1 | B1APG1_HUMAN | cAMP-dependent protein kinase catal... | PRKACB | 197 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 80 | V → G in CAI56774 (Ref. 3) Curated | 1 | |
Sequence conflicti | 159 | H → N in AAH35058 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 163 | L → I in AAH35058 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 233 | A → V in CAI56774 (Ref. 3) Curated | 1 | |
Sequence conflicti | 261 | H → N in AAH35058 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_040594 | 106 | R → Q1 PublicationCorresponds to variant dbSNP:rs36117118Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017364 | 1 – 16 | MGNAA…EVESV → MAAYREPPCNQYTGTTTALQ KLEGFASRLFHRHSKGTAHD QKTALENDSLHFSEHTALWD RSM in isoform 2. 2 PublicationsAdd BLAST | 16 | |
Alternative sequenceiVSP_017365 | 1 – 15 | MGNAA…SEVES → MSARKSSDASACSSSEISDS F in isoform 6. 2 PublicationsAdd BLAST | 15 | |
Alternative sequenceiVSP_043372 | 1 – 15 | MGNAA…SEVES → MGLSRKSSDASACSSSEISD SF in isoform 9. 1 PublicationAdd BLAST | 15 | |
Alternative sequenceiVSP_017366 | 1 – 14 | MGNAA…GSEVE → MSARKSSDASACSSSEI in isoform 5 and isoform 10. 2 PublicationsAdd BLAST | 14 | |
Alternative sequenceiVSP_017367 | 1 – 14 | MGNAA…GSEVE → MGLSRKSSDASACSSSEI in isoform 7. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_017368 | 1 – 13 | Missing in isoform 4. 1 PublicationAdd BLAST | 13 | |
Alternative sequenceiVSP_017369 | 1 – 12 | Missing in isoform 3. 2 PublicationsAdd BLAST | 12 | |
Alternative sequenceiVSP_017370 | 13 – 16 | VESV → MGLL in isoform 3. 2 Publications | 4 | |
Alternative sequenceiVSP_017371 | 14 | E → M in isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_046238 | 79 – 111 | Missing in isoform 10. 1 PublicationAdd BLAST | 33 | |
Alternative sequenceiVSP_036556 | 256 – 257 | VR → NF in isoform 8. 1 Publication | 2 | |
Alternative sequenceiVSP_036557 | 258 – 351 | Missing in isoform 8. 1 PublicationAdd BLAST | 94 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
SeattleSNPs |
Sequence databases
3D structure databases
SMRi | P22694 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111554, 105 interactors |
IntActi | P22694, 73 interactors |
MINTi | P22694 |
STRINGi | 9606.ENSP00000359719 |
Chemistry databases
BindingDBi | P22694 |
ChEMBLi | CHEMBL2918 |
DrugBanki | DB12010, Fostamatinib DB02482, Phosphonothreonine |
DrugCentrali | P22694 |
GuidetoPHARMACOLOGYi | 1477 |
PTM databases
iPTMneti | P22694 |
MetOSitei | P22694 |
PhosphoSitePlusi | P22694 |
SwissPalmi | P22694 |
Genetic variation databases
BioMutai | PRKACB |
DMDMi | 125210 |
Proteomic databases
EPDi | P22694 |
jPOSTi | P22694 |
MassIVEi | P22694 |
MaxQBi | P22694 |
PaxDbi | P22694 |
PeptideAtlasi | P22694 |
PRIDEi | P22694 |
ProteomicsDBi | 3296 54019 [P22694-1] 54020 [P22694-2] 54021 [P22694-3] 54022 [P22694-4] 54023 [P22694-5] 54024 [P22694-6] 54025 [P22694-7] 54026 [P22694-8] 54027 [P22694-9] |
Protocols and materials databases
Antibodypediai | 4160, 398 antibodies |
DNASUi | 5567 |
Genome annotation databases
Organism-specific databases
CTDi | 5567 |
DisGeNETi | 5567 |
GeneCardsi | PRKACB |
HGNCi | HGNC:9381, PRKACB |
HPAi | ENSG00000142875, Tissue enhanced (brain) |
MIMi | 176892, gene |
neXtProti | NX_P22694 |
OpenTargetsi | ENSG00000142875 |
PharmGKBi | PA33749 |
VEuPathDBi | HostDB:ENSG00000142875.19 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0616, Eukaryota |
GeneTreei | ENSGT00940000161169 |
HOGENOMi | CLU_000288_63_5_1 |
InParanoidi | P22694 |
OMAi | SSLFDHY |
OrthoDBi | 963519at2759 |
PhylomeDBi | P22694 |
TreeFami | TF313399 |
Enzyme and pathway databases
BRENDAi | 2.7.11.11, 2681 |
PathwayCommonsi | P22694 |
Reactomei | R-HSA-111931, PKA-mediated phosphorylation of CREB R-HSA-163358, PKA-mediated phosphorylation of key metabolic factors R-HSA-163560, Triglyceride catabolism R-HSA-163615, PKA activation R-HSA-164378, PKA activation in glucagon signalling R-HSA-180024, DARPP-32 events R-HSA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-HSA-392517, Rap1 signalling R-HSA-422356, Regulation of insulin secretion R-HSA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-HSA-4420097, VEGFA-VEGFR2 Pathway R-HSA-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-HSA-5610780, Degradation of GLI1 by the proteasome R-HSA-5610783, Degradation of GLI2 by the proteasome R-HSA-5610785, GLI3 is processed to GLI3R by the proteasome R-HSA-5610787, Hedgehog 'off' state R-HSA-5621575, CD209 (DC-SIGN) signaling R-HSA-5687128, MAPK6/MAPK4 signaling R-HSA-8853659, RET signaling R-HSA-8963896, HDL assembly R-HSA-9010642, ROBO receptors bind AKAP5 R-HSA-9634600, Regulation of glycolysis by fructose 2,6-bisphosphate metabolism R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production R-HSA-9664323, FCGR3A-mediated IL10 synthesis R-HSA-983231, Factors involved in megakaryocyte development and platelet production |
SABIO-RKi | P22694 |
SignaLinki | P22694 |
SIGNORi | P22694 |
Miscellaneous databases
BioGRID-ORCSi | 5567, 8 hits in 1022 CRISPR screens |
ChiTaRSi | PRKACB, human |
GeneWikii | PRKACB |
GenomeRNAii | 5567 |
Pharosi | P22694, Tchem |
PROi | PR:P22694 |
RNActi | P22694, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000142875, Expressed in caudate nucleus and 254 other tissues |
ExpressionAtlasi | P22694, baseline and differential |
Genevisiblei | P22694, HS |
Family and domain databases
CDDi | cd14209, STKc_PKA, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR044109, STKc_PKA |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KAPCB_HUMAN | |
Accessioni | P22694Primary (citable) accession number: P22694 Secondary accession number(s): B1APG4 Q96B09 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 217 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families